Reviewed, UniProtKB/Swiss-Prot P16657 (FABI_SALTY)

Names and origin

Protein names

Recommended name:
    Enoyl-[acyl-carrier-protein] reductase [NADH]
    EC=1.3.1.9
Alternative name(s):
    NADH-dependent enoyl-ACP reductase

Gene names

Name:	fabI
Synonyms:	envM
Ordered Locus Names:	STM1700

Organism

Salmonella typhimurium [Complete proteome] [HAMAP]

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	262 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Acyl-[acyl-carrier-protein] + NAD+ = trans-2,3-dehydroacyl-[acyl-carrier-protein] + NADH.
Pathway	Lipid metabolism; fatty acid biosynthesis.
Subcellular location	Cell inner membrane; Peripheral membrane protein.
Miscellaneous	The antibiotic diazaborine interferes with the activity by binding to the protein.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily.

Ontologies

Keywords
Biological process	Antibiotic resistance Fatty acid biosynthesis Lipid synthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro enoyl-[acyl-carrier-protein] reductase (NADH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.3
	Chain	2 – 262	261	Enoyl-[acyl-carrier-protein] reductase [NADH]		PRO_0000054910
Regions
	Nucleotide binding	10 – 36	27	NAD By similarity
Sites
	Active site	156	1	Proton acceptor By similarity
Experimental info
	Mutagenesis	93	1	G → S: Diazaborine resistance.

Sequences

	Sequence		Length	Mass (Da)
	P16657-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 185A199EB359F643	FASTA	262	27,761
	10 20 30 40 50 60 MGFLSGKRIL VTGVASKLSI AYGIAQAMHR EGAELAFTYQ NDKLKGRVEE FAAQLGSSIV 70 80 90 100 110 120 LPCDVAEDAS IDAMFAELGN VWPKFDGFVH SIGFAPGDQL DGDYVNAVTR EGFKVAHDIS 130 140 150 160 170 180 SYSFVAMAKA CRTMLNPGSA LLTLSYLGAE RAIPNYNVMG LAKASLEANV RYMANAMGPE 190 200 210 220 230 240 GVRVNAISAG PIRTLAASGI KDFRKMLAHC EAVTPIRRTV TIEDVGNSAA FLCSDLSAGI 250 260 SGEVVHVDGG FSIAAMNELE LK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"envM genes of Salmonella typhimurium and Escherichia coli." Turnowsky F., Fuchs K., Jeschek C., Hoegenauer G. J. Bacteriol. 171:6555-6565(1989) [PubMed: 2687243] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: AG701.
[2]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.
[3]	"Salmonella typhimurium responses to a bactericidal protein from human neutrophils." Qi S.Y., Li Y., Szyroki A., Giles I.G., Moir A., O'Connor C.D. Mol. Microbiol. 17:523-531(1995) [PubMed: 8559071] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-11. Strain: SL1344.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M31806 Genomic DNA. Translation: AAA27059.1. AE006468 Genomic DNA. Translation: AAL20618.1.
PIR	B43729.
RefSeq	NP_460659.1.
3D structure databases
SMR	P16657. Positions 2-260.
ModBase	Search...
Proteomic databases
PRIDE	P16657.
Genome annotation databases
GeneID	1253219.
GenomeReviews	Gene locus STM1700 in contig AE006468_GR.
KEGG	stm:STM1700.
NMPDR	fig|99287.1.peg.1646.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG750976.
OMA	LAKVWPK.
Enzyme and pathway databases
BioCyc	STYP99287:STM1700-MONOMER.
BRENDA	1.3.1.9. 2.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR014358. Enoyl-ACP_Rdtase_NADH. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit. PTHR19410:SF12. Enoyl-ACP_rdct. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH.
ProtoNet	Search...

Entry information

Entry name

FABI_SALTY

Accession

Primary (citable) accession number: P16657

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	January 23, 2007
Last modified:	January 19, 2010
This is version 88 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)