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P16657

- FABI_SALTY

UniProt

P16657 - FABI_SALTY

Protein

Enoyl-[acyl-carrier-protein] reductase [NADH] FabI

Gene

fabI

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Involved in the elongation cycle of fatty acid which are used in the lipid metabolism and in the biotin biosynthesis By similarity.By similarity

    Catalytic activityi

    An acyl-[acyl-carrier protein] + NAD+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei13 – 131NAD; via carbonyl oxygenBy similarity
    Binding sitei40 – 401NADBy similarity
    Binding sitei92 – 921NAD; via carbonyl oxygenBy similarity
    Binding sitei95 – 951Substrate; via amide nitrogen and carbonyl oxygenBy similarity
    Active sitei146 – 1461Proton acceptorBy similarity
    Active sitei156 – 1561Proton acceptorBy similarity
    Binding sitei163 – 1631NADBy similarity
    Sitei201 – 2011Involved in acyl-ACP bindingBy similarity
    Sitei204 – 2041Involved in acyl-ACP bindingBy similarity
    Sitei205 – 2051Involved in acyl-ACP bindingBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi19 – 202NADBy similarity
    Nucleotide bindingi64 – 652NADBy similarity
    Nucleotide bindingi192 – 1965NADBy similarity

    GO - Molecular functioni

    1. enoyl-[acyl-carrier-protein] reductase (NADH) activity Source: UniProtKB

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB
    2. fatty acid elongation Source: UniProtKB
    3. protein homotetramerization Source: UniProtKB
    4. response to antibiotic Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Antibiotic resistance, Biotin biosynthesis, Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciSENT99287:GCTI-1709-MONOMER.
    UniPathwayiUPA00078.
    UPA00094.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Enoyl-[acyl-carrier-protein] reductase [NADH] FabI (EC:1.3.1.9)
    Short name:
    ENR
    Alternative name(s):
    NADH-dependent enoyl-ACP reductase
    Gene namesi
    Name:fabI
    Synonyms:envM
    Ordered Locus Names:STM1700
    OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
    Taxonomic identifieri99287 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
    ProteomesiUP000001014: Chromosome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi93 – 931G → S: Diazaborine resistance. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 262261Enoyl-[acyl-carrier-protein] reductase [NADH] FabIPRO_0000054910Add
    BLAST

    Proteomic databases

    PaxDbiP16657.
    PRIDEiP16657.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi99287.STM1700.

    Structurei

    3D structure databases

    ProteinModelPortaliP16657.
    SMRiP16657. Positions 2-260.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0623.
    KOiK00208.
    OMAiKLSIAWA.
    OrthoDBiEOG6HF644.
    PhylomeDBiP16657.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR014358. Enoyl-ACP_Rdtase_NADH.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PIRSFiPIRSF000094. Enoyl-ACP_rdct. 1 hit.
    PRINTSiPR00081. GDHRDH.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P16657-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGFLSGKRIL VTGVASKLSI AYGIAQAMHR EGAELAFTYQ NDKLKGRVEE    50
    FAAQLGSSIV LPCDVAEDAS IDAMFAELGN VWPKFDGFVH SIGFAPGDQL 100
    DGDYVNAVTR EGFKVAHDIS SYSFVAMAKA CRTMLNPGSA LLTLSYLGAE 150
    RAIPNYNVMG LAKASLEANV RYMANAMGPE GVRVNAISAG PIRTLAASGI 200
    KDFRKMLAHC EAVTPIRRTV TIEDVGNSAA FLCSDLSAGI SGEVVHVDGG 250
    FSIAAMNELE LK 262
    Length:262
    Mass (Da):27,761
    Last modified:January 23, 2007 - v3
    Checksum:i185A199EB359F643
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M31806 Genomic DNA. Translation: AAA27059.1.
    AE006468 Genomic DNA. Translation: AAL20618.1.
    PIRiB43729.
    RefSeqiNP_460659.1. NC_003197.1.

    Genome annotation databases

    EnsemblBacteriaiAAL20618; AAL20618; STM1700.
    GeneIDi1253219.
    KEGGistm:STM1700.
    PATRICi32381909. VBISalEnt20916_1794.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M31806 Genomic DNA. Translation: AAA27059.1 .
    AE006468 Genomic DNA. Translation: AAL20618.1 .
    PIRi B43729.
    RefSeqi NP_460659.1. NC_003197.1.

    3D structure databases

    ProteinModelPortali P16657.
    SMRi P16657. Positions 2-260.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 99287.STM1700.

    Proteomic databases

    PaxDbi P16657.
    PRIDEi P16657.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL20618 ; AAL20618 ; STM1700 .
    GeneIDi 1253219.
    KEGGi stm:STM1700.
    PATRICi 32381909. VBISalEnt20916_1794.

    Phylogenomic databases

    eggNOGi COG0623.
    KOi K00208.
    OMAi KLSIAWA.
    OrthoDBi EOG6HF644.
    PhylomeDBi P16657.

    Enzyme and pathway databases

    UniPathwayi UPA00078 .
    UPA00094 .
    BioCyci SENT99287:GCTI-1709-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR014358. Enoyl-ACP_Rdtase_NADH.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PIRSFi PIRSF000094. Enoyl-ACP_rdct. 1 hit.
    PRINTSi PR00081. GDHRDH.
    ProtoNeti Search...

    Publicationsi

    1. "envM genes of Salmonella typhimurium and Escherichia coli."
      Turnowsky F., Fuchs K., Jeschek C., Hoegenauer G.
      J. Bacteriol. 171:6555-6565(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF GLY-93.
      Strain: AG701.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: LT2 / SGSC1412 / ATCC 700720.
    3. "Salmonella typhimurium responses to a bactericidal protein from human neutrophils."
      Qi S.Y., Li Y., Szyroki A., Giles I.G., Moir A., O'Connor C.D.
      Mol. Microbiol. 17:523-531(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-11.
      Strain: SL1344.

    Entry informationi

    Entry nameiFABI_SALTY
    AccessioniPrimary (citable) accession number: P16657
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 120 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The antibiotic diazaborine interferes with the activity by binding to the protein.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3