Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

General transcriptional corepressor TUP1

Gene

TUP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as component of the CYC8-TUP1 corepressor complex which is involved in the repression of many genes in a wide variety of physiological processes including heme-regulated and catabolite repressed genes. May also be involved in the derepression of at least some target genes. The complex is recruited to target genes by interaction with DNA-bound transcriptional repressors, like MATALPHA2, MIG1, RFX1 and SKO1. The complex recruits histone deacetylases to produce a repressive chromatin structure, interacts with hypoacetylated N-terminal tails of histones H3 and H4 that have been programmed for repression by the action of histone deacetylases and interferes directly with the transcriptional machinery by associating with the RNA polymerase II mediator complex.7 Publications

GO - Molecular functioni

  • histone binding Source: SGD
  • histone deacetylase binding Source: SGD
  • mediator complex binding Source: SGD
  • phosphatidylinositol-3,5-bisphosphate binding Source: SGD
  • transcriptional repressor activity, RNA polymerase II transcription factor binding Source: SGD

GO - Biological processi

  • histone exchange Source: SGD
  • negative regulation of dipeptide transport by negative regulation of transcription from RNA polymerase II promoter Source: SGD
  • negative regulation of mating-type specific transcription from RNA polymerase II promoter Source: SGD
  • negative regulation of transcription from RNA polymerase II promoter Source: SGD
  • negative regulation of transcription from RNA polymerase II promoter by glucose Source: SGD
  • negative regulation of transcription from RNA polymerase II promoter during mitosis Source: SGD
  • nucleosome positioning Source: SGD
  • regulation of fatty acid biosynthetic process by regulation of transcription from RNA polymerase II promoter Source: SGD
  • regulation of invasive growth in response to glucose limitation Source: SGD
  • regulation of response to DNA damage stimulus Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-29380-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
General transcriptional corepressor TUP1
Alternative name(s):
Flocculation suppressor protein
Glucose repression regulatory protein TUP1
Repressor AER2
Gene namesi
Name:TUP1
Synonyms:AAR1, AER2, AMM1, CYC9, FLK1, SFL2, UMR7
Ordered Locus Names:YCR084C
ORF Names:YCR84C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:YCR084C.
SGDiS000000680. TUP1.

Subcellular locationi

GO - Cellular componenti

  • Elongator holoenzyme complex Source: GO_Central
  • histone acetyltransferase complex Source: GO_Central
  • nucleus Source: SGD
  • transcriptional repressor complex Source: SGD
  • transcription elongation factor complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000513121 – 713General transcriptional corepressor TUP1Add BLAST713

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei439PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP16649.
PRIDEiP16649.

PTM databases

iPTMnetiP16649.

Interactioni

Subunit structurei

Associates with CYC8/SSN6 to form the CYC8-TUP1 (or TUP1-SSN6) corepressor complex that is composed of 4 copies of TUP1 and one copy of CYC8. Interacts with histone H3, histone H4, MATALPHA2, RFX1, SKO1, PGD1, HDA1, HDA2, HOS1, HOS2 AND RPD3.9 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CYC8P149225EBI-19654,EBI-18215
PGD1P403563EBI-19654,EBI-13268
RFX1P487432EBI-19654,EBI-15036
RPD3P325612EBI-19654,EBI-15864

GO - Molecular functioni

  • histone binding Source: SGD
  • histone deacetylase binding Source: SGD
  • mediator complex binding Source: SGD

Protein-protein interaction databases

BioGridi31057. 99 interactors.
DIPiDIP-512N.
IntActiP16649. 16 interactors.
MINTiMINT-648551.

Structurei

Secondary structure

1713
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni19 – 26Combined sources8
Helixi27 – 84Combined sources58
Beta strandi290 – 292Combined sources3
Helixi300 – 303Combined sources4
Beta strandi307 – 310Combined sources4
Beta strandi314 – 317Combined sources4
Beta strandi322 – 325Combined sources4
Beta strandi333 – 342Combined sources10
Beta strandi349 – 352Combined sources4
Beta strandi356 – 362Combined sources7
Beta strandi367 – 371Combined sources5
Turni372 – 374Combined sources3
Beta strandi377 – 381Combined sources5
Beta strandi445 – 451Combined sources7
Beta strandi455 – 462Combined sources8
Beta strandi467 – 471Combined sources5
Turni472 – 475Combined sources4
Beta strandi476 – 481Combined sources6
Beta strandi488 – 493Combined sources6
Beta strandi497 – 504Combined sources8
Beta strandi507 – 513Combined sources7
Turni514 – 517Combined sources4
Beta strandi518 – 524Combined sources7
Beta strandi529 – 534Combined sources6
Beta strandi541 – 546Combined sources6
Beta strandi551 – 555Combined sources5
Turni556 – 558Combined sources3
Beta strandi561 – 565Combined sources5
Beta strandi579 – 584Combined sources6
Beta strandi588 – 595Combined sources8
Beta strandi598 – 604Combined sources7
Beta strandi622 – 627Combined sources6
Beta strandi633 – 638Combined sources6
Helixi640 – 642Combined sources3
Beta strandi644 – 649Combined sources6
Beta strandi652 – 658Combined sources7
Turni659 – 661Combined sources3
Beta strandi664 – 669Combined sources6
Beta strandi675 – 680Combined sources6
Beta strandi691 – 697Combined sources7
Beta strandi700 – 709Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ERJX-ray2.30A/B/C282-388[»]
A/B/C432-713[»]
3VP8X-ray1.91A/B/C/D1-92[»]
3VP9X-ray1.80A/B1-92[»]
ProteinModelPortaliP16649.
SMRiP16649.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP16649.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati342 – 371WD 1Add BLAST30
Repeati441 – 471WD 2Add BLAST31
Repeati483 – 513WD 3Add BLAST31
Repeati524 – 555WD 4Add BLAST32
Repeati574 – 604WD 5Add BLAST31
Repeati628 – 658WD 6Add BLAST31
Repeati670 – 706WD 7Add BLAST37

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi97 – 118Gln-richAdd BLAST22
Compositional biasi181 – 198Poly-GlnAdd BLAST18
Compositional biasi399 – 409Thr-richAdd BLAST11

Sequence similaritiesi

Belongs to the WD repeat TUP1 family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

GeneTreeiENSGT00850000132350.
HOGENOMiHOG000200558.
InParanoidiP16649.
KOiK06666.
OMAiGKKSSCD.
OrthoDBiEOG092C21A7.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR013890. Tscrpt_rep_Tup1_N.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF08581. Tup_N. 1 hit.
PF00400. WD40. 7 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
ProDomiPD010558. Transcrip_repressor_Tup1_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P16649-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTASVSNTQN KLNELLDAIR QEFLQVSQEA NTYRLQNQKD YDFKMNQQLA
60 70 80 90 100
EMQQIRNTVY ELELTHRKMK DAYEEEIKHL KLGLEQRDHQ IASLTVQQQR
110 120 130 140 150
QQQQQQQVQQ HLQQQQQQLA AASASVPVAQ QPPATTSATA TPAANTTTGS
160 170 180 190 200
PSAFPVQASR PNLVGSQLPT TTLPVVSSNA QQQLPQQQLQ QQQLQQQQPP
210 220 230 240 250
PQVSVAPLSN TAINGSPTSK ETTTLPSVKA PESTLKETEP ENNNTSKIND
260 270 280 290 300
TGSATTATTT TATETEIKPK EEDATPASLH QDHYLVPYNQ RANHSKPIPP
310 320 330 340 350
FLLDLDSQSV PDALKKQTND YYILYNPALP REIDVELHKS LDHTSVVCCV
360 370 380 390 400
KFSNDGEYLA TGCNKTTQVY RVSDGSLVAR LSDDSAANNH RNSITENNTT
410 420 430 440 450
TSTDNNTMTT TTTTTITTTA MTSAAELAKD VENLNTSSSP SSDLYIRSVC
460 470 480 490 500
FSPDGKFLAT GAEDRLIRIW DIENRKIVMI LQGHEQDIYS LDYFPSGDKL
510 520 530 540 550
VSGSGDRTVR IWDLRTGQCS LTLSIEDGVT TVAVSPGDGK YIAAGSLDRA
560 570 580 590 600
VRVWDSETGF LVERLDSENE SGTGHKDSVY SVVFTRDGQS VVSGSLDRSV
610 620 630 640 650
KLWNLQNANN KSDSKTPNSG TCEVTYIGHK DFVLSVATTQ NDEYILSGSK
660 670 680 690 700
DRGVLFWDKK SGNPLLMLQG HRNSVISVAV ANGSPLGPEY NVFATGSGDC
710
KARIWKYKKI APN
Length:713
Mass (Da):78,308
Last modified:May 1, 1991 - v2
Checksum:i444104AAD63CB944
GO

Sequence cautioni

The sequence CAA34411 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti75E → A in AAA35182 (PubMed:2247069).Curated1
Sequence conflicti100R → Q in AAA35182 (PubMed:2247069).Curated1
Sequence conflicti685P → S in AAA35182 (PubMed:2247069).Curated1
Sequence conflicti685P → S in AAA34413 (PubMed:1900249).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31733 Genomic DNA. Translation: AAA35182.1.
M35861 Genomic DNA. Translation: AAA34413.1.
X16365 Genomic DNA. Translation: CAA34411.1. Different initiation.
X59720 Genomic DNA. Translation: CAA42259.1.
BK006937 Genomic DNA. Translation: DAA07554.1.
PIRiJN0133.
RefSeqiNP_010007.1. NM_001178790.1.

Genome annotation databases

EnsemblFungiiCAA42259; CAA42259; CAA42259.
YCR084C; YCR084C; YCR084C.
GeneIDi850445.
KEGGisce:YCR084C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31733 Genomic DNA. Translation: AAA35182.1.
M35861 Genomic DNA. Translation: AAA34413.1.
X16365 Genomic DNA. Translation: CAA34411.1. Different initiation.
X59720 Genomic DNA. Translation: CAA42259.1.
BK006937 Genomic DNA. Translation: DAA07554.1.
PIRiJN0133.
RefSeqiNP_010007.1. NM_001178790.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ERJX-ray2.30A/B/C282-388[»]
A/B/C432-713[»]
3VP8X-ray1.91A/B/C/D1-92[»]
3VP9X-ray1.80A/B1-92[»]
ProteinModelPortaliP16649.
SMRiP16649.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31057. 99 interactors.
DIPiDIP-512N.
IntActiP16649. 16 interactors.
MINTiMINT-648551.

PTM databases

iPTMnetiP16649.

Proteomic databases

MaxQBiP16649.
PRIDEiP16649.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAA42259; CAA42259; CAA42259.
YCR084C; YCR084C; YCR084C.
GeneIDi850445.
KEGGisce:YCR084C.

Organism-specific databases

EuPathDBiFungiDB:YCR084C.
SGDiS000000680. TUP1.

Phylogenomic databases

GeneTreeiENSGT00850000132350.
HOGENOMiHOG000200558.
InParanoidiP16649.
KOiK06666.
OMAiGKKSSCD.
OrthoDBiEOG092C21A7.

Enzyme and pathway databases

BioCyciYEAST:G3O-29380-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP16649.
PROiP16649.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR013890. Tscrpt_rep_Tup1_N.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF08581. Tup_N. 1 hit.
PF00400. WD40. 7 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
ProDomiPD010558. Transcrip_repressor_Tup1_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTUP1_YEAST
AccessioniPrimary (citable) accession number: P16649
Secondary accession number(s): D6VR85, P17995, P18323
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: May 1, 1991
Last modified: November 30, 2016
This is version 180 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5840 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.