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P16641

- KTRC_SCHMA

UniProt

P16641 - KTRC_SCHMA

Protein

Taurocyamine kinase

Gene

Smp_194770

Organism
Schistosoma mansoni (Blood fluke)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 3 (13 Jun 2012)
      Previous versions | rss
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    Functioni

    This family of enzymes reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate).1 Publication

    Catalytic activityi

    ATP + taurocyamine = ADP + N-phosphotaurocyamine.1 Publication

    Cofactori

    Magnesium.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei212 – 2121ATPPROSITE-ProRule annotation
    Binding sitei256 – 2561ATPPROSITE-ProRule annotation
    Active sitei298 – 2981PROSITE-ProRule annotation
    Binding sitei575 – 5751ATPPROSITE-ProRule annotation
    Binding sitei619 – 6191ATPPROSITE-ProRule annotation
    Active sitei661 – 6611PROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi149 – 1535ATPPROSITE-ProRule annotation
    Nucleotide bindingi307 – 3115ATPPROSITE-ProRule annotation
    Nucleotide bindingi335 – 3406ATPPROSITE-ProRule annotation
    Nucleotide bindingi512 – 5165ATPPROSITE-ProRule annotation
    Nucleotide bindingi670 – 6745ATPPROSITE-ProRule annotation
    Nucleotide bindingi699 – 7046ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. oxidoreductase activity Source: UniProtKB-KW
    4. taurocyamine kinase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Kinase, Oxidoreductase, Transferase

    Keywords - Ligandi

    ATP-binding, Flavoprotein, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Taurocyamine kinase (EC:2.7.3.4)
    Alternative name(s):
    ATP:guanidino kinase Smc74
    ATP:guanidino phosphotransferase
    Short name:
    SmGK
    Gene namesi
    ORF Names:Smp_194770
    OrganismiSchistosoma mansoni (Blood fluke)
    Taxonomic identifieri6183 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaPlatyhelminthesTrematodaDigeneaStrigeididaSchistosomatoideaSchistosomatidaeSchistosoma
    ProteomesiUP000008854: Chromosome 1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 746746Taurocyamine kinasePRO_0000212009Add
    BLAST

    Expressioni

    Developmental stagei

    Expressed at low levels in 37-day sporocysts, increases 25-fold in the infective cercarial and early schistosomular stages. Levels decrease dramatically in adults, adult males have approximately twice the expression level as in adult females.1 Publication

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati31 – 393363ApproximateAdd
    BLAST
    Domaini35 – 11682Phosphagen kinase N-terminal 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini146 – 382237Phosphagen kinase C-terminal 1PROSITE-ProRule annotationAdd
    BLAST
    Repeati394 – 705312ApproximateAdd
    BLAST
    Domaini398 – 47982Phosphagen kinase N-terminal 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini509 – 746238Phosphagen kinase C-terminal 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ATP:guanido phosphotransferase family.PROSITE-ProRule annotation
    Contains 2 phosphagen kinase C-terminal domains.PROSITE-ProRule annotation
    Contains 2 phosphagen kinase N-terminal domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    HOGENOMiHOG000006584.

    Family and domain databases

    Gene3Di1.10.135.10. 2 hits.
    3.30.590.10. 2 hits.
    InterProiIPR022415. ATP-guanido_PTrfase_AS.
    IPR022414. ATP-guanido_PTrfase_cat.
    IPR022413. ATP-guanido_PTrfase_N.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    [Graphical view]
    PfamiPF00217. ATP-gua_Ptrans. 2 hits.
    PF02807. ATP-gua_PtransN. 2 hits.
    [Graphical view]
    SUPFAMiSSF48034. SSF48034. 2 hits.
    PROSITEiPS00112. PHOSPHAGEN_KINASE. 2 hits.
    PS51510. PHOSPHAGEN_KINASE_C. 2 hits.
    PS51509. PHOSPHAGEN_KINASE_N. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P16641-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTCTSAFIKV VRFIQVVIDT REIRSLCTIR MQVESLQNLQ AKIRNDERNH    50
    SLTKKYLTDD IVKKYQATKT SLGGTLAQCV NTNAYNPGAL LPRSCDLNAY 100
    ETFRDFFDAV IADYHKVPDG KIQHPKSNFG DLKSLSFTDL NTYGNLVVST 150
    RVRLGRTVEG FGFGPTLTKE TRIELENKIS TALHNLSGEY EGTYYPLTGM 200
    SEEDRIKLVN DHFLFRNDDN VLRDAGGYID WPTGRGIFIN KQKNFLVWIN 250
    EEDHIRVISM QKGGDLIAVY KRLADAIQEL SKSLKFAFND RLGFITFCPS 300
    NLGTTLRASV HAKIPMLASL PNFKEICEKH GIQPRGTHGE HTESVGGIYD 350
    LSNKRRLGLT ELDAVTEMHS GVRALLELEV MLQEYNKGAP EGVMPVEPLT 400
    YLAKLLEGAS IEKCYTRKYL TPEIIKKYDG KRTTHGATLA HMIRNGAYNN 450
    RSICPRTGEA ECYSTFIDYL DPLICDYHGV KDSAFKHPAP TFGDLSKLPF 500
    GDLDPTGKFI VSTRVRVGRS VEDFLFPTIM SKTDRIKLEQ VISGALKGLT 550
    GEHAGTYYPL TDMKEEDRKQ LVEDHFLFKN DDPVLRDAGG YRDWPVGRGI 600
    FHNNSKTFLV WVCEEDHMRI ISMQQGGNLA AVYKRLIEGI NAIGKSMKFA 650
    HSDKYGYITC CPSNLGTSMR ASVLLKIPKL SSQPKKLDEI CAKYMLQARG 700
    LYGEHTESPD GTYDISNKRR LGLTELQAAH EMAEGVAKMI EIEKGL 746
    Length:746
    Mass (Da):83,891
    Last modified:June 13, 2012 - v3
    Checksum:i03B55FFD75E979B1
    GO

    Sequence cautioni

    The sequence AAA29927.1 differs from that shown. Reason: Frameshift at positions 416, 434 and 644.
    The sequence CCD76533.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti45 – 451N → S in ABU49846. (PubMed:18083248)Curated
    Sequence conflicti200 – 21112MSEED…KLVND → CQRGQNQTSKRH in AAA29927. (PubMed:2324092)CuratedAdd
    BLAST
    Sequence conflicti244 – 2441N → K in AAA29927. (PubMed:2324092)Curated
    Sequence conflicti264 – 2641G → R in AAA29927. (PubMed:2324092)Curated
    Sequence conflicti282 – 2821K → R in ABU49846. (PubMed:18083248)Curated
    Sequence conflicti523 – 5231D → G in AAA29927. (PubMed:2324092)Curated
    Sequence conflicti523 – 5231D → G no nucleotide entry (PubMed:18765922)Curated
    Sequence conflicti523 – 5231D → G in ABU49845. (PubMed:22253936)Curated
    Sequence conflicti632 – 6321V → A no nucleotide entry (PubMed:18765922)Curated
    Sequence conflicti681 – 74666SSQPK…IEKGL → VLNRKSWMKFVRSDMPSKLE VLLW in AAA29927. (PubMed:2324092)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05410 mRNA. Translation: AAA29927.1. Frameshift.
    HE601624 Genomic DNA. Translation: CCD76533.1. Sequence problems.
    EU042595 mRNA. Translation: ABU49845.1.
    EU042596 mRNA. Translation: ABU49846.1.
    L31768 Genomic DNA. Translation: AAA29912.1.
    L31769 Genomic DNA. Translation: AAA29913.1.
    PIRiA35743.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05410 mRNA. Translation: AAA29927.1 . Frameshift.
    HE601624 Genomic DNA. Translation: CCD76533.1 . Sequence problems.
    EU042595 mRNA. Translation: ABU49845.1 .
    EU042596 mRNA. Translation: ABU49846.1 .
    L31768 Genomic DNA. Translation: AAA29912.1 .
    L31769 Genomic DNA. Translation: AAA29913.1 .
    PIRi A35743.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    HOGENOMi HOG000006584.

    Family and domain databases

    Gene3Di 1.10.135.10. 2 hits.
    3.30.590.10. 2 hits.
    InterProi IPR022415. ATP-guanido_PTrfase_AS.
    IPR022414. ATP-guanido_PTrfase_cat.
    IPR022413. ATP-guanido_PTrfase_N.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    [Graphical view ]
    Pfami PF00217. ATP-gua_Ptrans. 2 hits.
    PF02807. ATP-gua_PtransN. 2 hits.
    [Graphical view ]
    SUPFAMi SSF48034. SSF48034. 2 hits.
    PROSITEi PS00112. PHOSPHAGEN_KINASE. 2 hits.
    PS51510. PHOSPHAGEN_KINASE_C. 2 hits.
    PS51509. PHOSPHAGEN_KINASE_N. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A cloned ATP:guanidino kinase in the trematode Schistosoma mansoni has a novel duplicated structure."
      Stein L.D., Harn D.A., David J.R.
      J. Biol. Chem. 265:6582-6588(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
      Strain: Puerto Rican.
    2. "Crystallization and X-ray analysis of the Schistosoma mansoni guanidino kinase."
      Awama A.M., Paracuellos P., Laurent S., Dissous C., Marcillat O., Gouet P.
      Acta Crystallogr. F 64:854-857(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS), FUNCTION, CATALYTIC ACTIVITY, COFACTOR.
    3. "The genome of the blood fluke Schistosoma mansoni."
      Berriman M., Haas B.J., LoVerde P.T., Wilson R.A., Dillon G.P., Cerqueira G.C., Mashiyama S.T., Al-Lazikani B., Andrade L.F., Ashton P.D., Aslett M.A., Bartholomeu D.C., Blandin G., Caffrey C.R., Coghlan A., Coulson R., Day T.A., Delcher A.
      , DeMarco R., Djikeng A., Eyre T., Gamble J.A., Ghedin E., Gu Y., Hertz-Fowler C., Hirai H., Hirai Y., Houston R., Ivens A., Johnston D.A., Lacerda D., Macedo C.D., McVeigh P., Ning Z., Oliveira G., Overington J.P., Parkhill J., Pertea M., Pierce R.J., Protasio A.V., Quail M.A., Rajandream M.A., Rogers J., Sajid M., Salzberg S.L., Stanke M., Tivey A.R., White O., Williams D.L., Wortman J., Wu W., Zamanian M., Zerlotini A., Fraser-Liggett C.M., Barrell B.G., El-Sayed N.M.
      Nature 460:352-358(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Puerto Rican.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Puerto Rican.
    5. "Molecular determinants of compatibility polymorphism in the Biomphalaria glabrata/Schistosoma mansoni model: New candidates identified by a global comparative proteomics approach."
      Roger E., Mitta G., Mone Y., Bouchut A., Rognon A., Grunau C., Boissier J., Theron A., Gourbal B.E.
      Mol. Biochem. Parasitol. 157:205-216(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-683.
      Strain: C and IC.
    6. "The Schistosoma mansoni phosphagen kinase gene contains two closely apposed transcription initiation sites and arose from a fused gene duplication."
      Shoemaker C.B.
      Mol. Biochem. Parasitol. 68:319-322(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-52 AND 362-415.

    Entry informationi

    Entry nameiKTRC_SCHMA
    AccessioniPrimary (citable) accession number: P16641
    Secondary accession number(s): A7UAX4
    , A7UAX5, C4QUJ5, G4VAV2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: June 13, 2012
    Last modified: October 1, 2014
    This is version 72 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3