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P16641

- KTRC_SCHMA

UniProt

P16641 - KTRC_SCHMA

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Protein

Taurocyamine kinase

Gene

Smp_194770

Organism
Schistosoma mansoni (Blood fluke)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

This family of enzymes reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate).1 Publication

Catalytic activityi

ATP + taurocyamine = ADP + N-phosphotaurocyamine.1 Publication

Cofactori

Mg2+1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei212 – 2121ATPPROSITE-ProRule annotation
Binding sitei256 – 2561ATPPROSITE-ProRule annotation
Active sitei298 – 2981PROSITE-ProRule annotation
Binding sitei575 – 5751ATPPROSITE-ProRule annotation
Binding sitei619 – 6191ATPPROSITE-ProRule annotation
Active sitei661 – 6611PROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi149 – 1535ATPPROSITE-ProRule annotation
Nucleotide bindingi307 – 3115ATPPROSITE-ProRule annotation
Nucleotide bindingi335 – 3406ATPPROSITE-ProRule annotation
Nucleotide bindingi512 – 5165ATPPROSITE-ProRule annotation
Nucleotide bindingi670 – 6745ATPPROSITE-ProRule annotation
Nucleotide bindingi699 – 7046ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. oxidoreductase activity Source: UniProtKB-KW
  4. taurocyamine kinase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Oxidoreductase, Transferase

Keywords - Ligandi

ATP-binding, Flavoprotein, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Taurocyamine kinase (EC:2.7.3.4)
Alternative name(s):
ATP:guanidino kinase Smc74
ATP:guanidino phosphotransferase
Short name:
SmGK
Gene namesi
ORF Names:Smp_194770
OrganismiSchistosoma mansoni (Blood fluke)
Taxonomic identifieri6183 [NCBI]
Taxonomic lineageiEukaryotaMetazoaPlatyhelminthesTrematodaDigeneaStrigeididaSchistosomatoideaSchistosomatidaeSchistosoma
ProteomesiUP000008854: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 746746Taurocyamine kinasePRO_0000212009Add
BLAST

Expressioni

Developmental stagei

Expressed at low levels in 37-day sporocysts, increases 25-fold in the infective cercarial and early schistosomular stages. Levels decrease dramatically in adults, adult males have approximately twice the expression level as in adult females.1 Publication

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati31 – 393363ApproximateAdd
BLAST
Domaini35 – 11682Phosphagen kinase N-terminal 1PROSITE-ProRule annotationAdd
BLAST
Domaini146 – 382237Phosphagen kinase C-terminal 1PROSITE-ProRule annotationAdd
BLAST
Repeati394 – 705312ApproximateAdd
BLAST
Domaini398 – 47982Phosphagen kinase N-terminal 2PROSITE-ProRule annotationAdd
BLAST
Domaini509 – 746238Phosphagen kinase C-terminal 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ATP:guanido phosphotransferase family.PROSITE-ProRule annotation
Contains 2 phosphagen kinase C-terminal domains.PROSITE-ProRule annotation
Contains 2 phosphagen kinase N-terminal domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000006584.
InParanoidiP16641.

Family and domain databases

Gene3Di1.10.135.10. 2 hits.
3.30.590.10. 2 hits.
InterProiIPR022415. ATP-guanido_PTrfase_AS.
IPR022414. ATP-guanido_PTrfase_cat.
IPR022413. ATP-guanido_PTrfase_N.
IPR014746. Gln_synth/guanido_kin_cat_dom.
[Graphical view]
PfamiPF00217. ATP-gua_Ptrans. 2 hits.
PF02807. ATP-gua_PtransN. 2 hits.
[Graphical view]
SUPFAMiSSF48034. SSF48034. 2 hits.
PROSITEiPS00112. PHOSPHAGEN_KINASE. 2 hits.
PS51510. PHOSPHAGEN_KINASE_C. 2 hits.
PS51509. PHOSPHAGEN_KINASE_N. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P16641-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTCTSAFIKV VRFIQVVIDT REIRSLCTIR MQVESLQNLQ AKIRNDERNH
60 70 80 90 100
SLTKKYLTDD IVKKYQATKT SLGGTLAQCV NTNAYNPGAL LPRSCDLNAY
110 120 130 140 150
ETFRDFFDAV IADYHKVPDG KIQHPKSNFG DLKSLSFTDL NTYGNLVVST
160 170 180 190 200
RVRLGRTVEG FGFGPTLTKE TRIELENKIS TALHNLSGEY EGTYYPLTGM
210 220 230 240 250
SEEDRIKLVN DHFLFRNDDN VLRDAGGYID WPTGRGIFIN KQKNFLVWIN
260 270 280 290 300
EEDHIRVISM QKGGDLIAVY KRLADAIQEL SKSLKFAFND RLGFITFCPS
310 320 330 340 350
NLGTTLRASV HAKIPMLASL PNFKEICEKH GIQPRGTHGE HTESVGGIYD
360 370 380 390 400
LSNKRRLGLT ELDAVTEMHS GVRALLELEV MLQEYNKGAP EGVMPVEPLT
410 420 430 440 450
YLAKLLEGAS IEKCYTRKYL TPEIIKKYDG KRTTHGATLA HMIRNGAYNN
460 470 480 490 500
RSICPRTGEA ECYSTFIDYL DPLICDYHGV KDSAFKHPAP TFGDLSKLPF
510 520 530 540 550
GDLDPTGKFI VSTRVRVGRS VEDFLFPTIM SKTDRIKLEQ VISGALKGLT
560 570 580 590 600
GEHAGTYYPL TDMKEEDRKQ LVEDHFLFKN DDPVLRDAGG YRDWPVGRGI
610 620 630 640 650
FHNNSKTFLV WVCEEDHMRI ISMQQGGNLA AVYKRLIEGI NAIGKSMKFA
660 670 680 690 700
HSDKYGYITC CPSNLGTSMR ASVLLKIPKL SSQPKKLDEI CAKYMLQARG
710 720 730 740
LYGEHTESPD GTYDISNKRR LGLTELQAAH EMAEGVAKMI EIEKGL
Length:746
Mass (Da):83,891
Last modified:June 13, 2012 - v3
Checksum:i03B55FFD75E979B1
GO

Sequence cautioni

The sequence AAA29927.1 differs from that shown. Reason: Frameshift at positions 416, 434 and 644. Curated
The sequence CCD76533.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti45 – 451N → S in ABU49846. (PubMed:18083248)Curated
Sequence conflicti200 – 21112MSEED…KLVND → CQRGQNQTSKRH in AAA29927. (PubMed:2324092)CuratedAdd
BLAST
Sequence conflicti244 – 2441N → K in AAA29927. (PubMed:2324092)Curated
Sequence conflicti264 – 2641G → R in AAA29927. (PubMed:2324092)Curated
Sequence conflicti282 – 2821K → R in ABU49846. (PubMed:18083248)Curated
Sequence conflicti523 – 5231D → G in AAA29927. (PubMed:2324092)Curated
Sequence conflicti523 – 5231D → G no nucleotide entry (PubMed:18765922)Curated
Sequence conflicti523 – 5231D → G in ABU49845. (PubMed:22253936)Curated
Sequence conflicti632 – 6321V → A no nucleotide entry (PubMed:18765922)Curated
Sequence conflicti681 – 74666SSQPK…IEKGL → VLNRKSWMKFVRSDMPSKLE VLLW in AAA29927. (PubMed:2324092)CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05410 mRNA. Translation: AAA29927.1. Frameshift.
HE601624 Genomic DNA. Translation: CCD76533.1. Sequence problems.
EU042595 mRNA. Translation: ABU49845.1.
EU042596 mRNA. Translation: ABU49846.1.
L31768 Genomic DNA. Translation: AAA29912.1.
L31769 Genomic DNA. Translation: AAA29913.1.
PIRiA35743.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05410 mRNA. Translation: AAA29927.1 . Frameshift.
HE601624 Genomic DNA. Translation: CCD76533.1 . Sequence problems.
EU042595 mRNA. Translation: ABU49845.1 .
EU042596 mRNA. Translation: ABU49846.1 .
L31768 Genomic DNA. Translation: AAA29912.1 .
L31769 Genomic DNA. Translation: AAA29913.1 .
PIRi A35743.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

HOGENOMi HOG000006584.
InParanoidi P16641.

Family and domain databases

Gene3Di 1.10.135.10. 2 hits.
3.30.590.10. 2 hits.
InterProi IPR022415. ATP-guanido_PTrfase_AS.
IPR022414. ATP-guanido_PTrfase_cat.
IPR022413. ATP-guanido_PTrfase_N.
IPR014746. Gln_synth/guanido_kin_cat_dom.
[Graphical view ]
Pfami PF00217. ATP-gua_Ptrans. 2 hits.
PF02807. ATP-gua_PtransN. 2 hits.
[Graphical view ]
SUPFAMi SSF48034. SSF48034. 2 hits.
PROSITEi PS00112. PHOSPHAGEN_KINASE. 2 hits.
PS51510. PHOSPHAGEN_KINASE_C. 2 hits.
PS51509. PHOSPHAGEN_KINASE_N. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A cloned ATP:guanidino kinase in the trematode Schistosoma mansoni has a novel duplicated structure."
    Stein L.D., Harn D.A., David J.R.
    J. Biol. Chem. 265:6582-6588(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
    Strain: Puerto Rican.
  2. "Crystallization and X-ray analysis of the Schistosoma mansoni guanidino kinase."
    Awama A.M., Paracuellos P., Laurent S., Dissous C., Marcillat O., Gouet P.
    Acta Crystallogr. F 64:854-857(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS), FUNCTION, CATALYTIC ACTIVITY, COFACTOR.
  3. "The genome of the blood fluke Schistosoma mansoni."
    Berriman M., Haas B.J., LoVerde P.T., Wilson R.A., Dillon G.P., Cerqueira G.C., Mashiyama S.T., Al-Lazikani B., Andrade L.F., Ashton P.D., Aslett M.A., Bartholomeu D.C., Blandin G., Caffrey C.R., Coghlan A., Coulson R., Day T.A., Delcher A.
    , DeMarco R., Djikeng A., Eyre T., Gamble J.A., Ghedin E., Gu Y., Hertz-Fowler C., Hirai H., Hirai Y., Houston R., Ivens A., Johnston D.A., Lacerda D., Macedo C.D., McVeigh P., Ning Z., Oliveira G., Overington J.P., Parkhill J., Pertea M., Pierce R.J., Protasio A.V., Quail M.A., Rajandream M.A., Rogers J., Sajid M., Salzberg S.L., Stanke M., Tivey A.R., White O., Williams D.L., Wortman J., Wu W., Zamanian M., Zerlotini A., Fraser-Liggett C.M., Barrell B.G., El-Sayed N.M.
    Nature 460:352-358(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Puerto Rican.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Puerto Rican.
  5. "Molecular determinants of compatibility polymorphism in the Biomphalaria glabrata/Schistosoma mansoni model: New candidates identified by a global comparative proteomics approach."
    Roger E., Mitta G., Mone Y., Bouchut A., Rognon A., Grunau C., Boissier J., Theron A., Gourbal B.E.
    Mol. Biochem. Parasitol. 157:205-216(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-683.
    Strain: C and IC.
  6. "The Schistosoma mansoni phosphagen kinase gene contains two closely apposed transcription initiation sites and arose from a fused gene duplication."
    Shoemaker C.B.
    Mol. Biochem. Parasitol. 68:319-322(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-52 AND 362-415.

Entry informationi

Entry nameiKTRC_SCHMA
AccessioniPrimary (citable) accession number: P16641
Secondary accession number(s): A7UAX4
, A7UAX5, C4QUJ5, G4VAV2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: June 13, 2012
Last modified: November 26, 2014
This is version 74 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3