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Protein

Putidaredoxin reductase

Gene

camA

Organism
Pseudomonas putida (Arthrobacter siderocapsulatus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The oxidation of camphor by cytochrome P450-CAM requires the participation of a flavoprotein, putidaredoxin reductase, and an iron-sulfur protein, putidaredoxin, to mediate the transfer of electrons from NADH to P450 for oxygen activation.1 Publication

Catalytic activityi

Reduced putidaredoxin + NAD+ = oxidized putidaredoxin + NADH.1 Publication

Cofactori

FAD2 Publications

Pathwayi: (R)-camphor degradation

This protein is involved in the pathway (R)-camphor degradation, which is part of Terpene metabolism.
View all proteins of this organism that are known to be involved in the pathway (R)-camphor degradation and in Terpene metabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi6 – 37FADSequence analysisAdd BLAST32
Nucleotide bindingi156 – 165NADSequence analysis10

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, NAD

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-3501.
BRENDAi1.18.1.5. 5092.
UniPathwayiUPA00719.

Names & Taxonomyi

Protein namesi
Recommended name:
Putidaredoxin reductase (EC:1.18.1.5)
Alternative name(s):
Putidaredoxin--NAD+ reductase
Gene namesi
Name:camA
OrganismiPseudomonas putida (Arthrobacter siderocapsulatus)
Taxonomic identifieri303 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Chemistry databases

DrugBankiDB03147. Flavin adenine dinucleotide.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001676461 – 422Putidaredoxin reductaseAdd BLAST422

Expressioni

Inductioni

By camphor.

Structurei

Secondary structure

1422
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 10Combined sources5
Helixi14 – 25Combined sources12
Beta strandi30 – 35Combined sources6
Helixi46 – 48Combined sources3
Turni49 – 55Combined sources7
Helixi60 – 62Combined sources3
Beta strandi63 – 65Combined sources3
Helixi67 – 72Combined sources6
Beta strandi75 – 78Combined sources4
Beta strandi83 – 87Combined sources5
Turni88 – 91Combined sources4
Beta strandi92 – 95Combined sources4
Beta strandi100 – 102Combined sources3
Beta strandi104 – 108Combined sources5
Beta strandi112 – 114Combined sources3
Helixi118 – 120Combined sources3
Helixi123 – 126Combined sources4
Beta strandi130 – 135Combined sources6
Helixi136 – 144Combined sources9
Beta strandi151 – 155Combined sources5
Helixi159 – 170Combined sources12
Beta strandi174 – 178Combined sources5
Beta strandi180 – 183Combined sources4
Turni184 – 188Combined sources5
Helixi191 – 204Combined sources14
Beta strandi207 – 209Combined sources3
Beta strandi214 – 219Combined sources6
Turni221 – 223Combined sources3
Beta strandi226 – 231Combined sources6
Beta strandi236 – 238Combined sources3
Beta strandi240 – 244Combined sources5
Beta strandi248 – 250Combined sources3
Helixi253 – 257Combined sources5
Beta strandi262 – 267Combined sources6
Beta strandi279 – 281Combined sources3
Helixi283 – 285Combined sources3
Beta strandi286 – 290Combined sources5
Turni291 – 294Combined sources4
Beta strandi295 – 298Combined sources4
Helixi302 – 316Combined sources15
Beta strandi328 – 333Combined sources6
Beta strandi336 – 342Combined sources7
Beta strandi348 – 355Combined sources8
Turni356 – 359Combined sources4
Beta strandi360 – 367Combined sources8
Beta strandi370 – 378Combined sources9
Helixi380 – 391Combined sources12
Helixi398 – 401Combined sources4
Helixi408 – 421Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Q1RX-ray1.91A/B1-422[»]
1Q1WX-ray2.60A/B1-422[»]
3LB8X-ray2.60A/B2-422[»]
ProteinModelPortaliP16640.
SMRiP16640.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP16640.

Family & Domainsi

Family and domain databases

Gene3Di3.30.390.30. 1 hit.
3.50.50.60. 2 hits.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR028202. Reductase_C.
[Graphical view]
PfamiPF07992. Pyr_redox_2. 1 hit.
PF14759. Reductase_C. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
SSF55424. SSF55424. 1 hit.

Sequencei

Sequence statusi: Complete.

P16640-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNANDNVVIV GTGLAGVEVA FGLRASGWEG NIRLVGDATV IPHHLPPLSK
60 70 80 90 100
AYLAGKATAE SLYLRTPDAY AAQNIQLLGG TQVTAINRDR QQVILSDGRA
110 120 130 140 150
LDYDRLVLAT GGRPRPLPVA SGAVGKANNF RYLRTLEDAE CIRRQLIADN
160 170 180 190 200
RLVVIGGGYI GLEVAATAIK ANMHVTLLDT AARVLERVTA PPVSAFYEHL
210 220 230 240 250
HREAGVDIRT GTQVCGFEMS TDQQKVTAVL CEDGTRLPAD LVIAGIGLIP
260 270 280 290 300
NCELASAAGL QVDNGIVINE HMQTSDPLIM AVGDCARFHS QLYDRWVRIE
310 320 330 340 350
SVPNALEQAR KIAAILCGKV PRDEAAPWFW SDQYEIGLKM VGLSEGYDRI
360 370 380 390 400
IVRGSLAQPD FSVFYLQGDR VLAVDTVNRP VEFNQSKQII TDRLPVEPNL
410 420
LGDESVPLKE IIAAAKAELS SA
Length:422
Mass (Da):45,579
Last modified:August 1, 1990 - v1
Checksum:i704BF52E47491AD1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05406 Genomic DNA. Translation: AAA25758.1.
D00528 Genomic DNA. Translation: BAA00413.1.
M12546 Genomic DNA. Translation: AAA25761.1.
PIRiJX0078.

Genome annotation databases

KEGGiag:AAA25758.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05406 Genomic DNA. Translation: AAA25758.1.
D00528 Genomic DNA. Translation: BAA00413.1.
M12546 Genomic DNA. Translation: AAA25761.1.
PIRiJX0078.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Q1RX-ray1.91A/B1-422[»]
1Q1WX-ray2.60A/B1-422[»]
3LB8X-ray2.60A/B2-422[»]
ProteinModelPortaliP16640.
SMRiP16640.
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

DrugBankiDB03147. Flavin adenine dinucleotide.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:AAA25758.

Enzyme and pathway databases

UniPathwayiUPA00719.
BioCyciMetaCyc:MONOMER-3501.
BRENDAi1.18.1.5. 5092.

Miscellaneous databases

EvolutionaryTraceiP16640.

Family and domain databases

Gene3Di3.30.390.30. 1 hit.
3.50.50.60. 2 hits.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR028202. Reductase_C.
[Graphical view]
PfamiPF07992. Pyr_redox_2. 1 hit.
PF14759. Reductase_C. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
SSF55424. SSF55424. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCAMA_PSEPU
AccessioniPrimary (citable) accession number: P16640
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: November 2, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.