Reviewed, UniProtKB/Swiss-Prot P16639 (ATE1_YEAST)

Names and origin

Protein names

Recommended name:
    Arginyl-tRNA--protein transferase 1
      Short name=Arginyltransferase 1
      Short name=R-transferase 1
    EC=2.3.2.8
Alternative name(s):
    Arginine-tRNA--protein transferase 1

Gene names

Name:	ATE1
Ordered Locus Names:	YGL017W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	503 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the post-translational conjugation of arginine to the N-terminal aspartate or glutamate of a protein. This arginylation is required for degradation of the protein via the ubiquitin pathway. Does not arginylate cysteine residues By similarity.
Catalytic activity	L-arginyl-tRNA + protein = tRNA + L-arginyl-protein.
Subcellular location	Cytoplasm.
Miscellaneous	Present with 2030 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the R-transferase family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein arginylation Ref.1 Inferred from direct assay. Source: SGD
Cellular component	cytoplasm Ref.1 Traceable author statement. Source: SGD
Molecular function	acyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW arginyltransferase activity Ref.1 Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 503	503	Arginyl-tRNA--protein transferase 1		PRO_0000195092
Experimental info
	Sequence conflict	122	1	T → S Ref.1
	Sequence conflict	122	1	T → S Ref.2
	Sequence conflict	402	1	Y → I in AAA34439. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P16639-1 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: 4A4A8F1099C0825F	FASTA	503	57,930
	10 20 30 40 50 60 MSDRFVIWAP SMHNEPAAKC GYCHGNKGGN MDQLFALDSW AHRYMNKMDV VKIENCTIGS 70 80 90 100 110 120 FVEHMDVATY DRMCNMGFRR SGKFLYKVDP LRNCCRLYTI RTAPQELNMT KELKKCISRF 130 140 150 160 170 180 ATRITSEDYC PAAVASSDFV GKIVNAEMNS KTFYTRFEPA LYSEEKYHLF VKYQEKVHQD 190 200 210 220 230 240 YNNSPKSFKR FLCDTPFGPE AVLGTQESWE QLNNWQRMKP GEKLKHMGPV HECYYYEGKL 250 260 270 280 290 300 IAITVSDILP SGISSVYFIW DPDYSKWSLG KLSALRDLAI IQRTNLQYYY LGYYIEDCPK 310 320 330 340 350 360 MNYKANYGAE VLDVCHSKYI PLKPIQDMIS RGKLFVIGEE ETKVTKELYL VDSETGRGEG 370 380 390 400 410 420 FPTDNVVKYK NIAEEIYGVG GCAFKSANES ALELKELYGI PYEEEDLDTI YHLKEHNGHA 430 440 450 460 470 480 PNGIPNVVPG LLPLWELLDI MQSGKITDLE GRLFLFEIET EGIRPLINFY SEPPNVKKRI 490 500 CDVIRLFGFE TCMKAVILYS EQM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and functional analysis of the arginyl-tRNA-protein transferase gene ATE1 of Saccharomyces cerevisiae." Balzi E., Choder M., Chen W., Varshavsky A., Goffeau A. J. Biol. Chem. 265:7464-7471(1990) [PubMed: 2185248] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The DNA sequencing of the 17 kb HindIII fragment spanning the LEU1 and ATE1 loci on chromosome VII from Saccharomyces cerevisiae reveals the PDR6 gene, a new member of the genetic network controlling pleiotropic drug resistance." Chen W., Balzi E., Capieaux E., Choder M., Goffeau A. Yeast 7:287-299(1991) [PubMed: 1882553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 46191 / IL125-2B.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J05404 Genomic DNA. Translation: AAA34439.1. S58126 Genomic DNA. Translation: AAD13904.1. Z72539 Genomic DNA. Translation: CAA96717.1.
PIR	S64019.
RefSeq	NP_011498.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:6572N.
IntAct	P16639. 2 interactions.
STRING	P16639.
Proteomic databases
PeptideAtlas	P16639.
Genome annotation databases
Ensembl	YGL017W; YGL017W; YGL017W; Saccharomyces cerevisiae. [Genome view]
GeneID	852867.
GenomeReviews	Gene locus YGL017W in contig Y13135_GR.
KEGG	sce:YGL017W.
NMPDR	fig|4932.3.peg.2605.
Organism-specific databases
CYGD	YGL017w.
SGD	S000002985. ATE1.
Phylogenomic databases
HOGENOM	P16639.
OMA	FIWDPDY.
Enzyme and pathway databases
BRENDA	2.3.2.8. 250.
Gene expression databases
ArrayExpress	P16639.
Genevestigator	P16639.
GermOnline	YGL017W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR017137. Arg-tRNA-P_Trfase_1_euk. IPR007472. Arg-tRNA-P_Trfase_C. IPR007471. Arg_tRNA_PTrfase_N. [Graphical view]
Pfam	PF04377. ATE_C. 1 hit. PF04376. ATE_N. 1 hit. [Graphical view]
PIRSF	PIRSF037207. ATE1_euk. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	972487.

Entry information

Entry name

ATE1_YEAST

Accession

Primary (citable) accession number: P16639

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	October 1, 1996
Last modified:	November 3, 2009
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)