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P16639 (ATE1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Arginyl-tRNA--protein transferase 1

Short name=Arginyltransferase 1
Short name=R-transferase 1
EC=2.3.2.8
Alternative name(s):
Arginine-tRNA--protein transferase 1
Gene names
Name:ATE1
Ordered Locus Names:YGL017W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length503 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the post-translational conjugation of arginine to the N-terminal aspartate or glutamate of a protein. This arginylation is required for degradation of the protein via the ubiquitin pathway. Does not arginylate cysteine residues By similarity.

Catalytic activity

L-arginyl-tRNA(Arg) + [protein] = tRNA(Arg) + L-arginyl-[protein].

Subcellular location

Cytoplasm.

Miscellaneous

Present with 2030 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the R-transferase family.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   Cellular componentCytoplasm
   Molecular functionAcyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processprotein arginylation

Inferred from direct assay Ref.1. Source: SGD

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionarginyltransferase activity

Inferred from direct assay Ref.1. Source: SGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 503503Arginyl-tRNA--protein transferase 1
PRO_0000195092

Experimental info

Sequence conflict1221T → S in AAA34439. Ref.1
Sequence conflict1221T → S in AAD13904. Ref.2
Sequence conflict4021Y → I in AAA34439. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P16639 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: 4A4A8F1099C0825F

FASTA50357,930
        10         20         30         40         50         60 
MSDRFVIWAP SMHNEPAAKC GYCHGNKGGN MDQLFALDSW AHRYMNKMDV VKIENCTIGS 

        70         80         90        100        110        120 
FVEHMDVATY DRMCNMGFRR SGKFLYKVDP LRNCCRLYTI RTAPQELNMT KELKKCISRF 

       130        140        150        160        170        180 
ATRITSEDYC PAAVASSDFV GKIVNAEMNS KTFYTRFEPA LYSEEKYHLF VKYQEKVHQD 

       190        200        210        220        230        240 
YNNSPKSFKR FLCDTPFGPE AVLGTQESWE QLNNWQRMKP GEKLKHMGPV HECYYYEGKL 

       250        260        270        280        290        300 
IAITVSDILP SGISSVYFIW DPDYSKWSLG KLSALRDLAI IQRTNLQYYY LGYYIEDCPK 

       310        320        330        340        350        360 
MNYKANYGAE VLDVCHSKYI PLKPIQDMIS RGKLFVIGEE ETKVTKELYL VDSETGRGEG 

       370        380        390        400        410        420 
FPTDNVVKYK NIAEEIYGVG GCAFKSANES ALELKELYGI PYEEEDLDTI YHLKEHNGHA 

       430        440        450        460        470        480 
PNGIPNVVPG LLPLWELLDI MQSGKITDLE GRLFLFEIET EGIRPLINFY SEPPNVKKRI 

       490        500 
CDVIRLFGFE TCMKAVILYS EQM 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and functional analysis of the arginyl-tRNA-protein transferase gene ATE1 of Saccharomyces cerevisiae."
Balzi E., Choder M., Chen W., Varshavsky A., Goffeau A.
J. Biol. Chem. 265:7464-7471(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The DNA sequencing of the 17 kb HindIII fragment spanning the LEU1 and ATE1 loci on chromosome VII from Saccharomyces cerevisiae reveals the PDR6 gene, a new member of the genetic network controlling pleiotropic drug resistance."
Chen W., Balzi E., Capieaux E., Choder M., Goffeau A.
Yeast 7:287-299(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 46191 / IL125-2B.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J05404 Genomic DNA. Translation: AAA34439.1.
S58126 Genomic DNA. Translation: AAD13904.1.
Z72539 Genomic DNA. Translation: CAA96717.1.
BK006941 Genomic DNA. Translation: DAA08081.1.
PIRS64019.
RefSeqNP_011498.1. NM_001180882.1.

3D structure databases

ProteinModelPortalP16639.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33229. 14 interactions.
DIPDIP-6572N.
IntActP16639. 2 interactions.
MINTMINT-685454.
STRING4932.YGL017W.

Proteomic databases

MaxQBP16639.
PaxDbP16639.
PeptideAtlasP16639.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGL017W; YGL017W; YGL017W.
GeneID852867.
KEGGsce:YGL017W.

Organism-specific databases

CYGDYGL017w.
SGDS000002985. ATE1.

Phylogenomic databases

eggNOGCOG2935.
GeneTreeENSGT00500000044926.
HOGENOMHOG000034123.
KOK00685.
OMAFLCDTPF.
OrthoDBEOG7FV40Z.

Enzyme and pathway databases

BioCycYEAST:YGL017W-MONOMER.

Gene expression databases

GenevestigatorP16639.

Family and domain databases

InterProIPR016181. Acyl_CoA_acyltransferase.
IPR017137. Arg-tRNA-P_Trfase_1_euk.
IPR007472. Arg-tRNA-P_Trfase_C.
IPR007471. Arg_tRNA_PTrfase_N.
[Graphical view]
PfamPF04377. ATE_C. 1 hit.
PF04376. ATE_N. 1 hit.
[Graphical view]
PIRSFPIRSF037207. ATE1_euk. 1 hit.
SUPFAMSSF55729. SSF55729. 1 hit.
ProtoNetSearch...

Other

NextBio972487.
PROP16639.

Entry information

Entry nameATE1_YEAST
AccessionPrimary (citable) accession number: P16639
Secondary accession number(s): D6VUC0
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: October 1, 1996
Last modified: May 14, 2014
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families