P16619 (CL3L1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
C-C motif chemokine 3-like 1

Alternative name(s):
G0/G1 switch regulatory protein 19-2
LD78-beta(1-70)
PAT 464.2
Small-inducible cytokine A3-like 1
Tonsillar lymphocyte LD78 beta protein

Cleaved into the following 2 chains:

1. LD78-beta(3-70)
2. LD78-beta(5-70)

Gene names

Name:	CCL3L1
Synonyms:	D17S1718, G0S19-2, SCYA3L1
AND
Name:	CCL3L3

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	93 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Chemotactic for lymphocytes and monocytes. Is a ligand for CCR1, CCR3 and CCR5. Is an inhibitor of HIV-1-infection. The processed form LD78-beta(3-70) shows a 20-fold to 30-fold higher chemotactic activity and is a very potent inhibitor of HIV-1-infection. LD78-beta(3-70) is also a ligand for CCR1, CCR3 and CCR5. Ref.7 Ref.8
Subcellular location	Secreted.
Post-translational modification	The N-terminal processed forms LD78-beta(3-70) and LD78-beta(5-70) are produced by proteolytic cleavage after secretion from peripheral blood monocytes. The cleavage to yield LD78-beta(3-70) is probably achieved by DPP4.
Polymorphism	The copy number of the CCL3L1 gene varies among individuals; most individuals have 1-6 copies in the diploid genome, although rare individuals have zero or more than 6 copies.
Sequence similarities	Belongs to the intercrine beta (chemokine CC) family.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Secreted
Domain	Signal
Molecular function	Cytokine
PTM	Disulfide bond
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	chemotaxis Non-traceable author statement. Source: UniProtKB immune response Inferred from electronic annotation. Source: InterPro inflammatory response Traceable author statement Ref.3. Source: UniProtKB negative regulation of cell proliferation Traceable author statement Ref.2. Source: UniProtKB
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chemokine activity Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Ref.6
	Chain	24 – 93	70	C-C motif chemokine 3-like 1		PRO_0000005161
	Chain	26 – 93	68	LD78-beta(3-70)		PRO_0000005162
	Chain	28 – 93	66	LD78-beta(5-70)		PRO_0000005163
Sites
	Site	25 – 26	2	Cleavage; by DPP4 Probable
Amino acid modifications
	Disulfide bond	34 ↔ 58		By similarity
	Disulfide bond	35 ↔ 74		By similarity
Experimental info
	Sequence conflict	91	1	L → P in AAP35429. Ref.4
	Sequence conflict	91	1	L → P in AAH07783. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P16619 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: A7A79E774006D61E	FASTA	93	10,161
	10 20 30 40 50 60 MQVSTAALAV LLCTMALCNQ VLSAPLAADT PTACCFSYTS RQIPQNFIAD YFETSSQCSK 70 80 90 PSVIFLTKRG RQVCADPSEE WVQKYVSDLE LSA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two inflammatory mediator cytokine genes are closely linked and variably amplified on chromosome 17q." Irving S.G., Zipfel P.F., Balke J., McBride O.W., Morton C.C., Burd P.R., Siebenlist U., Kelly K. Nucleic Acids Res. 18:3261-3270(1990) [PubMed: 1972563] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: T-cell.
[2]	"Three human homologs of a murine gene encoding an inhibitor of stem cell proliferation." Blum S., Forsdyke R.E., Forsdyke D.R. DNA Cell Biol. 9:589-602(1990) [PubMed: 2271120] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Structures of human genes coding for cytokine LD78 and their expression." Nakao M., Nomiyama H., Shimada K. Mol. Cell. Biol. 10:3646-3658(1990) [PubMed: 1694014] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: B-cell, Brain, Pancreas and Spleen.
[6]	"Signal peptide prediction based on analysis of experimentally verified cleavage sites." Zhang Z., Henzel W.J. Protein Sci. 13:2819-2824(2004) [PubMed: 15340161] [Abstract] Cited for: PROTEIN SEQUENCE OF 24-38.
[7]	"Cleavage by CD26/dipeptidyl peptidase IV converts the chemokine LD78beta into a most efficient monocyte attractant and CCR1 agonist." Proost P., Menten P., Struyf S., Schutyser E., De Meester I., Van Damme J. Blood 96:1674-1680(2000) [PubMed: 10961862] [Abstract] Cited for: IDENTIFICATION OF LD78-BETA(3-70) AND LD78-BETA(5-70), PROTEOLYTIC PROCESSING OF N-TERMINAL, FUNCTION.
[8]	"Diverging binding capacities of natural LD78beta isoforms of macrophage inflammatory protein-1alpha to the CC chemokine receptors 1, 3 and 5 affect their anti-HIV-1 activity and chemotactic potencies for neutrophils and eosinophils." Struyf S., Menten P., Lenaerts J.-P., Put W., D'Haese A., De Clercq E., Schols D., Proost P., Van Damme J. Eur. J. Immunol. 31:2170-2178(2001) [PubMed: 11449371] [Abstract] Cited for: FUNCTION.
[9]	"CCL3L1 and CCL4L1: variable gene copy number in adolescents with and without human immunodeficiency virus type 1 (HIV-1) infection." Shao W., Tang J., Song W., Wang C., Li Y., Wilson C.M., Kaslow R.A. Genes Immun. 8:224-231(2007) [PubMed: 17330138] [Abstract] Cited for: POLYMORPHISM OF GENE COPY NUMBER.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X52149 mRNA. Translation: CAA36397.1. M24110 Genomic DNA. Translation: AAA35859.1. D90145 Genomic DNA. Translation: BAA14173.1. BT006783 mRNA. Translation: AAP35429.1. BC007783 mRNA. Translation: AAH07783.1. BC027888 mRNA. Translation: AAH27888.1. BC107710 mRNA. Translation: AAI07711.1. BC146914 mRNA. Translation: AAI46915.1. BC146919 mRNA. Translation: AAI46920.1. BC147008 mRNA. Translation: AAI47009.1. BC147013 mRNA. Translation: AAI47014.1.
IPI	IPI00029732.
PIR	B35673.
RefSeq	NP_001001437.2. NM_001001437.3. NP_066286.1. NM_021006.4.
UniGene	Hs.512304. Hs.512683.
3D structure databases
ProteinModelPortal	P16619.
SMR	P16619. Positions 27-92.
ModBase	Search...
Protein-protein interaction databases
STRING	P16619.
Proteomic databases
PRIDE	P16619.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000378354; ENSP00000367605; ENSG00000205021. ENST00000422211; ENSP00000408984; ENSG00000205021. ENST00000425833; ENSP00000396037; ENSG00000205021.
GeneID	414062. 6349.
KEGG	hsa:414062. hsa:6349.
UCSC	uc002hlf.1. human.
Organism-specific databases
CTD	414062. 6349.
GeneCards	GC17M034523. GC17M034623.
HGNC	HGNC:10628. CCL3L1. HGNC:30554. CCL3L3.
MIM	601395. gene. 609468. gene.
neXtProt	NX_P16619.
PharmGKB	PA35560.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG21306.
GeneTree	ENSGT00560000077010.
HOGENOM	HBG505826.
HOVERGEN	HBG017871.
InParanoid	P16619.
OMA	QIPRKFI.
OrthoDB	EOG42Z4RR.
PhylomeDB	P16619.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
CleanEx	HS_CCL3L1. HS_CCL3L3.
Genevestigator	P16619.
GermOnline	ENSG00000205017. Homo sapiens. ENSG00000205021. Homo sapiens.
Family and domain databases
InterPro	IPR000827. Chemokine_CC_CS. IPR001811. Chemokine_IL8-like_dom. [Graphical view]
KO	K05408.
Pfam	PF00048. IL8. 1 hit. [Graphical view]
SMART	SM00199. SCY. 1 hit. [Graphical view]
SUPFAM	SSF54117. Chemokine_IL8. 1 hit.
PROSITE	PS00472. SMALL_CYTOKINES_CC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	108388.
PMAP-CutDB	P16619.
SOURCE	Search...

Entry information

Entry name

CL3L1_HUMAN

Accession

Primary (citable) accession number: P16619
Secondary accession number(s): A6NGK6 , B2RUX4, Q3B7A8, Q53YA5, Q96I68

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	January 25, 2012
This is version 122 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.