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P16617

- PGK1_RAT

UniProt

P16617 - PGK1_RAT

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Protein

Phosphoglycerate kinase 1

Gene

Pgk1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei39 – 391SubstrateBy similarity
Binding sitei123 – 1231SubstrateBy similarity
Binding sitei171 – 1711SubstrateBy similarity
Binding sitei220 – 2201ATPBy similarity
Binding sitei313 – 3131ATP; via carbonyl oxygenBy similarity
Binding sitei344 – 3441ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi373 – 3764ATPBy similarity

GO - Molecular functioni

  1. ADP binding Source: RGD
  2. ATP binding Source: UniProtKB
  3. phosphoglycerate kinase activity Source: UniProtKB

GO - Biological processi

  1. carbohydrate metabolic process Source: RGD
  2. gluconeogenesis Source: RGD
  3. glycolytic process Source: RGD
  4. phosphorylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_217998. Gluconeogenesis.
REACT_225694. Glycolysis.
SABIO-RKP16617.
UniPathwayiUPA00109; UER00185.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglycerate kinase 1 (EC:2.7.2.3)
Gene namesi
Name:Pgk1
Synonyms:Pgk-1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome X

Organism-specific databases

RGDi619878. Pgk1.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytosol Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 417416Phosphoglycerate kinase 1PRO_0000145840Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei6 – 61N6-succinyllysineBy similarity
Modified residuei11 – 111N6-acetyllysineBy similarity
Modified residuei48 – 481N6-acetyllysine; alternateBy similarity
Modified residuei48 – 481N6-succinyllysine; alternateBy similarity
Modified residuei75 – 751N6-acetyllysineBy similarity
Modified residuei76 – 761PhosphotyrosineBy similarity
Modified residuei86 – 861N6-acetyllysineBy similarity
Modified residuei91 – 911N6-acetyllysineBy similarity
Modified residuei97 – 971N6-acetyllysineBy similarity
Modified residuei131 – 1311N6-acetyllysine; alternateBy similarity
Modified residuei131 – 1311N6-malonyllysine; alternateBy similarity
Modified residuei146 – 1461N6-acetyllysineBy similarity
Modified residuei191 – 1911N6-succinyllysineBy similarity
Modified residuei196 – 1961PhosphotyrosineBy similarity
Modified residuei199 – 1991N6-acetyllysineBy similarity
Modified residuei203 – 2031PhosphoserineBy similarity
Modified residuei267 – 2671N6-acetyllysineBy similarity
Modified residuei291 – 2911N6-acetyllysineBy similarity
Modified residuei361 – 3611N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP16617.
PRIDEiP16617.

2D gel databases

World-2DPAGE0004:P16617.

PTM databases

PhosphoSiteiP16617.

Expressioni

Gene expression databases

GenevestigatoriP16617.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi246779. 3 interactions.
IntActiP16617. 2 interactions.
MINTiMINT-4577521.

Structurei

3D structure databases

ProteinModelPortaliP16617.
SMRiP16617. Positions 2-417.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni24 – 263Substrate bindingBy similarity
Regioni63 – 664Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the phosphoglycerate kinase family.Curated

Phylogenomic databases

eggNOGiCOG0126.
GeneTreeiENSGT00390000008820.
HOGENOMiHOG000227107.
HOVERGENiHBG008177.
InParanoidiP16617.
KOiK00927.
OMAiASCCAKW.
OrthoDBiEOG74R1QN.
PhylomeDBiP16617.
TreeFamiTF300489.

Family and domain databases

Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P16617-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSLSNKLTLD KLDVKGKRVV MRVDFNVPMK NNQITNNQRI KAAVPSIKFC
60 70 80 90 100
LDNGAKSVVL MSHLGRPDGV PMPDKYSLEP VAAELKSLLG KDVLFLKDCV
110 120 130 140 150
GSEVENACAN PAAGTVILLE NLRFHVEEEG KGKDASGNKV KAEPAKIDAF
160 170 180 190 200
RASLSKLGDV YVNDAFGTAH RAHSSMVGVN LPQKAGGFLM KKELNYFAKA
210 220 230 240 250
LESPERPFLA ILGGAKVADK IQLINNMLDK VNEMIIGGGM AFTFLKVLNN
260 270 280 290 300
MEIGTSLYDE EGAKIVKDLM AKAEKNGVKI TLPVDFVTAD KFDENAKTGQ
310 320 330 340 350
ATVASGIPAG WMGLDCGTES SKKYAEAVAR AKQIVWNGPV GVFEWEAFAR
360 370 380 390 400
GTKSLMDEVV KATSRGCITI IGGGDTATCC AKWNTEDKVS HVSTGGGASL
410
ELLEGKVLPG VDALSNV
Length:417
Mass (Da):44,538
Last modified:January 23, 2007 - v2
Checksum:i58E6D9CA85550A20
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti56 – 561K → N in AAA41838. (PubMed:2610697)Curated
Sequence conflicti271 – 2711A → T in AAA41838. (PubMed:2610697)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M31788 mRNA. Translation: AAA41838.1.
BC063161 mRNA. Translation: AAH63161.1.
BC087651 mRNA. Translation: AAH87651.1.
PIRiA33792.
RefSeqiNP_445743.2. NM_053291.3.
UniGeneiRn.108127.

Genome annotation databases

EnsembliENSRNOT00000003390; ENSRNOP00000003390; ENSRNOG00000002467.
GeneIDi24644.
KEGGirno:24644.
UCSCiRGD:619878. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M31788 mRNA. Translation: AAA41838.1 .
BC063161 mRNA. Translation: AAH63161.1 .
BC087651 mRNA. Translation: AAH87651.1 .
PIRi A33792.
RefSeqi NP_445743.2. NM_053291.3.
UniGenei Rn.108127.

3D structure databases

ProteinModelPortali P16617.
SMRi P16617. Positions 2-417.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 246779. 3 interactions.
IntActi P16617. 2 interactions.
MINTi MINT-4577521.

Chemistry

ChEMBLi CHEMBL2176815.

PTM databases

PhosphoSitei P16617.

2D gel databases

World-2DPAGE 0004:P16617.

Proteomic databases

PaxDbi P16617.
PRIDEi P16617.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000003390 ; ENSRNOP00000003390 ; ENSRNOG00000002467 .
GeneIDi 24644.
KEGGi rno:24644.
UCSCi RGD:619878. rat.

Organism-specific databases

CTDi 5230.
RGDi 619878. Pgk1.

Phylogenomic databases

eggNOGi COG0126.
GeneTreei ENSGT00390000008820.
HOGENOMi HOG000227107.
HOVERGENi HBG008177.
InParanoidi P16617.
KOi K00927.
OMAi ASCCAKW.
OrthoDBi EOG74R1QN.
PhylomeDBi P16617.
TreeFami TF300489.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00185 .
Reactomei REACT_217998. Gluconeogenesis.
REACT_225694. Glycolysis.
SABIO-RK P16617.

Miscellaneous databases

NextBioi 603950.
PROi P16617.

Gene expression databases

Genevestigatori P16617.

Family and domain databases

Gene3Di 3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPi MF_00145. Phosphoglyc_kinase.
InterProi IPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view ]
PANTHERi PTHR11406. PTHR11406. 1 hit.
Pfami PF00162. PGK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000724. Pgk. 1 hit.
PRINTSi PR00477. PHGLYCKINASE.
SUPFAMi SSF53748. SSF53748. 1 hit.
PROSITEi PS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and cDNA sequence of the rat X-chromosome linked phosphoglycerate kinase."
    Ciccarese S., Tommasi S., Vonghia G.
    Biochem. Biophys. Res. Commun. 165:1337-1344(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Ovary and Pituitary.
  3. Lubec G., Afjehi-Sadat L., Diao W.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 98-123; 157-171; 200-216; 280-297 AND 333-350, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Hippocampus and Spinal cord.

Entry informationi

Entry nameiPGK1_RAT
AccessioniPrimary (citable) accession number: P16617
Secondary accession number(s): Q5M945, Q6P508
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: January 23, 2007
Last modified: October 1, 2014
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3