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P16617

- PGK1_RAT

UniProt

P16617 - PGK1_RAT

Protein

Phosphoglycerate kinase 1

Gene

Pgk1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei39 – 391SubstrateBy similarity
    Binding sitei123 – 1231SubstrateBy similarity
    Binding sitei171 – 1711SubstrateBy similarity
    Binding sitei220 – 2201ATPBy similarity
    Binding sitei313 – 3131ATP; via carbonyl oxygenBy similarity
    Binding sitei344 – 3441ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi373 – 3764ATPBy similarity

    GO - Molecular functioni

    1. ADP binding Source: RGD
    2. ATP binding Source: UniProtKB
    3. phosphoglycerate kinase activity Source: UniProtKB

    GO - Biological processi

    1. carbohydrate metabolic process Source: RGD
    2. gluconeogenesis Source: RGD
    3. glycolytic process Source: RGD
    4. phosphorylation Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_217998. Gluconeogenesis.
    REACT_225694. Glycolysis.
    SABIO-RKP16617.
    UniPathwayiUPA00109; UER00185.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoglycerate kinase 1 (EC:2.7.2.3)
    Gene namesi
    Name:Pgk1
    Synonyms:Pgk-1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome X

    Organism-specific databases

    RGDi619878. Pgk1.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytosol Source: RGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 417416Phosphoglycerate kinase 1PRO_0000145840Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei6 – 61N6-succinyllysineBy similarity
    Modified residuei11 – 111N6-acetyllysineBy similarity
    Modified residuei48 – 481N6-acetyllysine; alternateBy similarity
    Modified residuei48 – 481N6-succinyllysine; alternateBy similarity
    Modified residuei75 – 751N6-acetyllysineBy similarity
    Modified residuei76 – 761PhosphotyrosineBy similarity
    Modified residuei86 – 861N6-acetyllysineBy similarity
    Modified residuei91 – 911N6-acetyllysineBy similarity
    Modified residuei97 – 971N6-acetyllysineBy similarity
    Modified residuei131 – 1311N6-acetyllysine; alternateBy similarity
    Modified residuei131 – 1311N6-malonyllysine; alternateBy similarity
    Modified residuei146 – 1461N6-acetyllysineBy similarity
    Modified residuei191 – 1911N6-succinyllysineBy similarity
    Modified residuei196 – 1961PhosphotyrosineBy similarity
    Modified residuei199 – 1991N6-acetyllysineBy similarity
    Modified residuei203 – 2031PhosphoserineBy similarity
    Modified residuei267 – 2671N6-acetyllysineBy similarity
    Modified residuei291 – 2911N6-acetyllysineBy similarity
    Modified residuei361 – 3611N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiP16617.
    PRIDEiP16617.

    2D gel databases

    World-2DPAGE0004:P16617.

    PTM databases

    PhosphoSiteiP16617.

    Expressioni

    Gene expression databases

    GenevestigatoriP16617.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    BioGridi246779. 3 interactions.
    IntActiP16617. 2 interactions.
    MINTiMINT-4577521.

    Structurei

    3D structure databases

    ProteinModelPortaliP16617.
    SMRiP16617. Positions 2-417.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni24 – 263Substrate bindingBy similarity
    Regioni63 – 664Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the phosphoglycerate kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0126.
    GeneTreeiENSGT00390000008820.
    HOGENOMiHOG000227107.
    HOVERGENiHBG008177.
    InParanoidiP16617.
    KOiK00927.
    OMAiASCCAKW.
    OrthoDBiEOG74R1QN.
    PhylomeDBiP16617.
    TreeFamiTF300489.

    Family and domain databases

    Gene3Di3.40.50.1260. 1 hit.
    3.40.50.1270. 1 hit.
    HAMAPiMF_00145. Phosphoglyc_kinase.
    InterProiIPR001576. Phosphoglycerate_kinase.
    IPR015901. Phosphoglycerate_kinase_C.
    IPR015911. Phosphoglycerate_kinase_CS.
    IPR015824. Phosphoglycerate_kinase_N.
    [Graphical view]
    PANTHERiPTHR11406. PTHR11406. 1 hit.
    PfamiPF00162. PGK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000724. Pgk. 1 hit.
    PRINTSiPR00477. PHGLYCKINASE.
    SUPFAMiSSF53748. SSF53748. 1 hit.
    PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P16617-1 [UniParc]FASTAAdd to Basket

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    MSLSNKLTLD KLDVKGKRVV MRVDFNVPMK NNQITNNQRI KAAVPSIKFC    50
    LDNGAKSVVL MSHLGRPDGV PMPDKYSLEP VAAELKSLLG KDVLFLKDCV 100
    GSEVENACAN PAAGTVILLE NLRFHVEEEG KGKDASGNKV KAEPAKIDAF 150
    RASLSKLGDV YVNDAFGTAH RAHSSMVGVN LPQKAGGFLM KKELNYFAKA 200
    LESPERPFLA ILGGAKVADK IQLINNMLDK VNEMIIGGGM AFTFLKVLNN 250
    MEIGTSLYDE EGAKIVKDLM AKAEKNGVKI TLPVDFVTAD KFDENAKTGQ 300
    ATVASGIPAG WMGLDCGTES SKKYAEAVAR AKQIVWNGPV GVFEWEAFAR 350
    GTKSLMDEVV KATSRGCITI IGGGDTATCC AKWNTEDKVS HVSTGGGASL 400
    ELLEGKVLPG VDALSNV 417
    Length:417
    Mass (Da):44,538
    Last modified:January 23, 2007 - v2
    Checksum:i58E6D9CA85550A20
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti56 – 561K → N in AAA41838. (PubMed:2610697)Curated
    Sequence conflicti271 – 2711A → T in AAA41838. (PubMed:2610697)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M31788 mRNA. Translation: AAA41838.1.
    BC063161 mRNA. Translation: AAH63161.1.
    BC087651 mRNA. Translation: AAH87651.1.
    PIRiA33792.
    RefSeqiNP_445743.2. NM_053291.3.
    UniGeneiRn.108127.

    Genome annotation databases

    EnsembliENSRNOT00000003390; ENSRNOP00000003390; ENSRNOG00000002467.
    GeneIDi24644.
    KEGGirno:24644.
    UCSCiRGD:619878. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M31788 mRNA. Translation: AAA41838.1 .
    BC063161 mRNA. Translation: AAH63161.1 .
    BC087651 mRNA. Translation: AAH87651.1 .
    PIRi A33792.
    RefSeqi NP_445743.2. NM_053291.3.
    UniGenei Rn.108127.

    3D structure databases

    ProteinModelPortali P16617.
    SMRi P16617. Positions 2-417.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 246779. 3 interactions.
    IntActi P16617. 2 interactions.
    MINTi MINT-4577521.

    Chemistry

    ChEMBLi CHEMBL2176815.

    PTM databases

    PhosphoSitei P16617.

    2D gel databases

    World-2DPAGE 0004:P16617.

    Proteomic databases

    PaxDbi P16617.
    PRIDEi P16617.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000003390 ; ENSRNOP00000003390 ; ENSRNOG00000002467 .
    GeneIDi 24644.
    KEGGi rno:24644.
    UCSCi RGD:619878. rat.

    Organism-specific databases

    CTDi 5230.
    RGDi 619878. Pgk1.

    Phylogenomic databases

    eggNOGi COG0126.
    GeneTreei ENSGT00390000008820.
    HOGENOMi HOG000227107.
    HOVERGENi HBG008177.
    InParanoidi P16617.
    KOi K00927.
    OMAi ASCCAKW.
    OrthoDBi EOG74R1QN.
    PhylomeDBi P16617.
    TreeFami TF300489.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00185 .
    Reactomei REACT_217998. Gluconeogenesis.
    REACT_225694. Glycolysis.
    SABIO-RK P16617.

    Miscellaneous databases

    NextBioi 603950.
    PROi P16617.

    Gene expression databases

    Genevestigatori P16617.

    Family and domain databases

    Gene3Di 3.40.50.1260. 1 hit.
    3.40.50.1270. 1 hit.
    HAMAPi MF_00145. Phosphoglyc_kinase.
    InterProi IPR001576. Phosphoglycerate_kinase.
    IPR015901. Phosphoglycerate_kinase_C.
    IPR015911. Phosphoglycerate_kinase_CS.
    IPR015824. Phosphoglycerate_kinase_N.
    [Graphical view ]
    PANTHERi PTHR11406. PTHR11406. 1 hit.
    Pfami PF00162. PGK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000724. Pgk. 1 hit.
    PRINTSi PR00477. PHGLYCKINASE.
    SUPFAMi SSF53748. SSF53748. 1 hit.
    PROSITEi PS00111. PGLYCERATE_KINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and cDNA sequence of the rat X-chromosome linked phosphoglycerate kinase."
      Ciccarese S., Tommasi S., Vonghia G.
      Biochem. Biophys. Res. Commun. 165:1337-1344(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Ovary and Pituitary.
    3. Lubec G., Afjehi-Sadat L., Diao W.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 98-123; 157-171; 200-216; 280-297 AND 333-350, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Hippocampus and Spinal cord.

    Entry informationi

    Entry nameiPGK1_RAT
    AccessioniPrimary (citable) accession number: P16617
    Secondary accession number(s): Q5M945, Q6P508
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 114 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3