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Protein

Phosphoglycerate kinase 1

Gene

Pgk1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein). May play a role in sperm motility.By similarity

Catalytic activityi

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.

Pathwayi: glycolysis

This protein is involved in step 2 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (Gapdh), Glyceraldehyde-3-phosphate dehydrogenase, Glyceraldehyde-3-phosphate dehydrogenase, Glyceraldehyde-3-phosphate dehydrogenase, testis-specific (Gapdhs), Glyceraldehyde-3-phosphate dehydrogenase, Glyceraldehyde-3-phosphate dehydrogenase, Glyceraldehyde-3-phosphate dehydrogenase (LOC108351137), Glyceraldehyde-3-phosphate dehydrogenase (Gapdh-ps2), Glyceraldehyde-3-phosphate dehydrogenase (Gapdhs), Glyceraldehyde-3-phosphate dehydrogenase (RGD1562758)
  2. Phosphoglycerate kinase 1 (Pgk1), Phosphoglycerate kinase, Phosphoglycerate kinase (Pgk2)
  3. no protein annotated in this organism
  4. Alpha-enolase (Eno1), Beta-enolase (Eno3), Gamma-enolase (Eno2)
  5. Pyruvate kinase PKLR (Pklr), Pyruvate kinase PKM (Pkm), Pyruvate kinase (Pklr), Pyruvate kinase (Pkm), Pyruvate kinase (Pkm), Pyruvate kinase (Pklr), Pyruvate kinase (Pkm)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei39SubstrateBy similarity1
Binding sitei123SubstrateBy similarity1
Binding sitei171SubstrateBy similarity1
Binding sitei220ATPBy similarity1
Binding sitei313ATP; via carbonyl oxygenBy similarity1
Binding sitei344ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi373 – 376ATPBy similarity4

GO - Molecular functioni

  • ADP binding Source: RGD
  • ATP binding Source: RGD
  • phosphoglycerate kinase activity Source: RGD
  • protein-disulfide reductase activity Source: RGD

GO - Biological processi

  • carbohydrate metabolic process Source: RGD
  • cellular response to hypoxia Source: RGD
  • epithelial cell differentiation Source: RGD
  • gluconeogenesis Source: RGD
  • glycolytic process Source: RGD
  • negative regulation of angiogenesis Source: RGD
  • phosphorylation Source: UniProtKB
  • plasminogen activation Source: RGD

Keywordsi

Molecular functionKinase, Transferase
Biological processGlycolysis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-70171. Glycolysis.
R-RNO-70263. Gluconeogenesis.
SABIO-RKiP16617.
UniPathwayiUPA00109; UER00185.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglycerate kinase 1 (EC:2.7.2.3)
Gene namesi
Name:Pgk1
Synonyms:Pgk-1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome X

Organism-specific databases

RGDi619878. Pgk1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2176815.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001458402 – 417Phosphoglycerate kinase 1Add BLAST416

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei2PhosphoserineBy similarity1
Modified residuei4PhosphoserineBy similarity1
Modified residuei6N6-succinyllysineBy similarity1
Modified residuei11N6-acetyllysineBy similarity1
Modified residuei48N6-acetyllysine; alternateBy similarity1
Modified residuei48N6-succinyllysine; alternateBy similarity1
Modified residuei75N6-acetyllysineBy similarity1
Modified residuei76PhosphotyrosineBy similarity1
Modified residuei86N6-acetyllysineBy similarity1
Modified residuei91N6-acetyllysineBy similarity1
Modified residuei97N6-acetyllysineBy similarity1
Modified residuei131N6-acetyllysine; alternateBy similarity1
Modified residuei131N6-malonyllysine; alternateBy similarity1
Modified residuei146N6-acetyllysineBy similarity1
Modified residuei191N6-succinyllysineBy similarity1
Modified residuei196PhosphotyrosineBy similarity1
Modified residuei199N6-acetyllysineBy similarity1
Modified residuei203PhosphoserineBy similarity1
Modified residuei267N6-acetyllysineBy similarity1
Modified residuei291N6-acetyllysineBy similarity1
Modified residuei354PhosphoserineCombined sources1
Modified residuei361N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP16617.
PRIDEiP16617.

2D gel databases

World-2DPAGEi0004:P16617.

PTM databases

iPTMnetiP16617.
PhosphoSitePlusiP16617.

Expressioni

Gene expression databases

BgeeiENSRNOG00000058249.
ExpressionAtlasiP16617. differential.
GenevisibleiP16617. RN.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi246779. 3 interactors.
IntActiP16617. 2 interactors.
MINTiMINT-4577521.
STRINGi10116.ENSRNOP00000003390.

Structurei

3D structure databases

ProteinModelPortaliP16617.
SMRiP16617.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni24 – 26Substrate bindingBy similarity3
Regioni63 – 66Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the phosphoglycerate kinase family.Curated

Phylogenomic databases

eggNOGiKOG1367. Eukaryota.
COG0126. LUCA.
GeneTreeiENSGT00390000008820.
HOGENOMiHOG000227107.
HOVERGENiHBG008177.
InParanoidiP16617.
KOiK00927.
OMAiDMIFDIG.
OrthoDBiEOG091G08S6.
PhylomeDBiP16617.
TreeFamiTF300489.

Family and domain databases

CDDicd00318. Phosphoglycerate_kinase. 1 hit.
Gene3Di3.40.50.1260. 2 hits.
HAMAPiMF_00145. Phosphoglyc_kinase. 1 hit.
InterProiView protein in InterPro
IPR001576. Phosphoglycerate_kinase.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
IPR036043. Phosphoglycerate_kinase_sf.
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiView protein in Pfam
PF00162. PGK. 1 hit.
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiView protein in PROSITE
PS00111. PGLYCERATE_KINASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P16617-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLSNKLTLD KLDVKGKRVV MRVDFNVPMK NNQITNNQRI KAAVPSIKFC
60 70 80 90 100
LDNGAKSVVL MSHLGRPDGV PMPDKYSLEP VAAELKSLLG KDVLFLKDCV
110 120 130 140 150
GSEVENACAN PAAGTVILLE NLRFHVEEEG KGKDASGNKV KAEPAKIDAF
160 170 180 190 200
RASLSKLGDV YVNDAFGTAH RAHSSMVGVN LPQKAGGFLM KKELNYFAKA
210 220 230 240 250
LESPERPFLA ILGGAKVADK IQLINNMLDK VNEMIIGGGM AFTFLKVLNN
260 270 280 290 300
MEIGTSLYDE EGAKIVKDLM AKAEKNGVKI TLPVDFVTAD KFDENAKTGQ
310 320 330 340 350
ATVASGIPAG WMGLDCGTES SKKYAEAVAR AKQIVWNGPV GVFEWEAFAR
360 370 380 390 400
GTKSLMDEVV KATSRGCITI IGGGDTATCC AKWNTEDKVS HVSTGGGASL
410
ELLEGKVLPG VDALSNV
Length:417
Mass (Da):44,538
Last modified:January 23, 2007 - v2
Checksum:i58E6D9CA85550A20
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti56K → N in AAA41838 (PubMed:2610697).Curated1
Sequence conflicti271A → T in AAA41838 (PubMed:2610697).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31788 mRNA. Translation: AAA41838.1.
BC063161 mRNA. Translation: AAH63161.1.
BC087651 mRNA. Translation: AAH87651.1.
PIRiA33792.
RefSeqiNP_445743.2. NM_053291.3.
UniGeneiRn.108127.

Genome annotation databases

EnsembliENSRNOT00000077604; ENSRNOP00000073523; ENSRNOG00000058249.
GeneIDi24644.
KEGGirno:24644.
UCSCiRGD:619878. rat.

Similar proteinsi

Entry informationi

Entry nameiPGK1_RAT
AccessioniPrimary (citable) accession number: P16617
Secondary accession number(s): Q5M945, Q6P508
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: January 23, 2007
Last modified: November 22, 2017
This is version 140 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families