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Protein

Porphobilinogen deaminase

Gene

hemC

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.By similarity

Catalytic activityi

4 porphobilinogen + H2O = hydroxymethylbilane + 4 NH3.

Cofactori

dipyrromethaneNote: Binds 1 dipyrromethane group covalently.

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Delta-aminolevulinic acid dehydratase (hemB)
  2. Porphobilinogen deaminase (hemC)
  3. Uroporphyrinogen-III synthase (hemD)
  4. Uroporphyrinogen decarboxylase (hemE)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processPorphyrin biosynthesis

Enzyme and pathway databases

BioCyciBSUB:BSU28150-MONOMER.
UniPathwayiUPA00251; UER00319.

Names & Taxonomyi

Protein namesi
Recommended name:
Porphobilinogen deaminase (EC:2.5.1.61)
Short name:
PBG
Alternative name(s):
Hydroxymethylbilane synthase
Short name:
HMBS
Pre-uroporphyrinogen synthase
Gene namesi
Name:hemC
Ordered Locus Names:BSU28150
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001429101 – 313Porphobilinogen deaminaseAdd BLAST313

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei242S-(dipyrrolylmethanemethyl)cysteineBy similarity1

Proteomic databases

PaxDbiP16616.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100015381.

Structurei

3D structure databases

ProteinModelPortaliP16616.
SMRiP16616.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HMBS family.Curated

Phylogenomic databases

eggNOGiENOG4105D6W. Bacteria.
COG0181. LUCA.
HOGENOMiHOG000228587.
InParanoidiP16616.
KOiK01749.
OMAiGAICIES.
PhylomeDBiP16616.

Family and domain databases

Gene3Di3.30.160.40. 1 hit.
HAMAPiMF_00260. Porphobil_deam. 1 hit.
InterProiView protein in InterPro
IPR000860. HemC.
IPR022419. Porphobilin_deaminase_cofac_BS.
IPR022417. Porphobilin_deaminase_N.
IPR022418. Porphobilinogen_deaminase_C.
PANTHERiPTHR11557. PTHR11557. 1 hit.
PfamiView protein in Pfam
PF01379. Porphobil_deam. 1 hit.
PF03900. Porphobil_deamC. 1 hit.
PIRSFiPIRSF001438. 4pyrrol_synth_OHMeBilane_synth. 1 hit.
PRINTSiPR00151. PORPHBDMNASE.
SUPFAMiSSF54782. SSF54782. 1 hit.
TIGRFAMsiTIGR00212. hemC. 1 hit.
PROSITEiView protein in PROSITE
PS00533. PORPHOBILINOGEN_DEAM. 1 hit.

Sequencei

Sequence statusi: Complete.

P16616-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMRTIKVGSR RSKLAMTQTK WVIQKLKEIN PSFAFEIKEI VTKGDRIVDV
60 70 80 90 100
TLSKVGGKGL FVKEIEQALL NEEIDMAVHS MKDMPAVLPE GLVIGCIPER
110 120 130 140 150
EDPRDALISK NRVKLSEMKK GAVIGTSSLR RSAQLLIERP DLTIKWIRGN
160 170 180 190 200
IDTRLQKLET EDYDAIILAA AGLSRMGWKQ DVVTEFLEPE RCLPAVGQGA
210 220 230 240 250
LAIECRESDE ELLALFSQFT DEYTKRTVLA ERAFLNAMEG GCQVPIAGYS
260 270 280 290 300
VLNGQDEIEM TGLVASPDGK IIFKETVTGN DPEEVGKRCA ALMADKGAKD
310
LIDRVKRELD EDGK
Length:314
Mass (Da):34,838
Last modified:May 26, 2009 - v3
Checksum:iA4F13170243E874D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57676 Genomic DNA. Translation: AAA22512.1.
AL009126 Genomic DNA. Translation: CAB14775.1.
PIRiC35252. IBBS.
RefSeqiNP_390693.1. NC_000964.3.
WP_010886587.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB14775; CAB14775; BSU28150.
GeneIDi937488.
KEGGibsu:BSU28150.
PATRICi18977506. VBIBacSub10457_2942.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57676 Genomic DNA. Translation: AAA22512.1.
AL009126 Genomic DNA. Translation: CAB14775.1.
PIRiC35252. IBBS.
RefSeqiNP_390693.1. NC_000964.3.
WP_010886587.1. NC_000964.3.

3D structure databases

ProteinModelPortaliP16616.
SMRiP16616.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100015381.

Proteomic databases

PaxDbiP16616.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB14775; CAB14775; BSU28150.
GeneIDi937488.
KEGGibsu:BSU28150.
PATRICi18977506. VBIBacSub10457_2942.

Phylogenomic databases

eggNOGiENOG4105D6W. Bacteria.
COG0181. LUCA.
HOGENOMiHOG000228587.
InParanoidiP16616.
KOiK01749.
OMAiGAICIES.
PhylomeDBiP16616.

Enzyme and pathway databases

UniPathwayiUPA00251; UER00319.
BioCyciBSUB:BSU28150-MONOMER.

Family and domain databases

Gene3Di3.30.160.40. 1 hit.
HAMAPiMF_00260. Porphobil_deam. 1 hit.
InterProiView protein in InterPro
IPR000860. HemC.
IPR022419. Porphobilin_deaminase_cofac_BS.
IPR022417. Porphobilin_deaminase_N.
IPR022418. Porphobilinogen_deaminase_C.
PANTHERiPTHR11557. PTHR11557. 1 hit.
PfamiView protein in Pfam
PF01379. Porphobil_deam. 1 hit.
PF03900. Porphobil_deamC. 1 hit.
PIRSFiPIRSF001438. 4pyrrol_synth_OHMeBilane_synth. 1 hit.
PRINTSiPR00151. PORPHBDMNASE.
SUPFAMiSSF54782. SSF54782. 1 hit.
TIGRFAMsiTIGR00212. hemC. 1 hit.
PROSITEiView protein in PROSITE
PS00533. PORPHOBILINOGEN_DEAM. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHEM3_BACSU
AccessioniPrimary (citable) accession number: P16616
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: May 26, 2009
Last modified: March 15, 2017
This is version 139 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The porphobilinogen subunits are added to the dipyrromethane group.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.