Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Vascular endothelial growth factor A

Gene

Vegfa

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. May play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Growth factor, Heparin-binding, Mitogen
Biological processAngiogenesis, Differentiation

Names & Taxonomyi

Protein namesi
Recommended name:
Vascular endothelial growth factor A
Short name:
VEGF-A
Alternative name(s):
Vascular permeability factor
Short name:
VPF
Gene namesi
Name:Vegfa
Synonyms:Vegf
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi619991. Vegfa.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 262 PublicationsAdd BLAST26
ChainiPRO_000002339027 – 214Vascular endothelial growth factor AAdd BLAST188

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi51 ↔ 93By similarity
Disulfide bondi76InterchainBy similarity
Disulfide bondi82 ↔ 127By similarity
Disulfide bondi85InterchainBy similarity
Disulfide bondi86 ↔ 129By similarity
Glycosylationi100N-linked (GlcNAc...) asparagine1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP16612.
PRIDEiP16612.

PTM databases

PhosphoSitePlusiP16612.

Expressioni

Tissue specificityi

Expressed in the pituitary, in brain, in particularly in supraoptic and paraventricular nuclei and the choroid plexus. Also found abundantly in the corpus luteum of the ovary and in kidney glomeruli. Expressed in the ductal epithelial cells of post-pubertal mammary glands. Expressed in the ductal and alveolar epithelial cells throughout the whole period of gestational evolution, lactation and involution.1 Publication

Developmental stagei

Increases during pregnancy (5.0-fold increase on day 12 with a subsequent decrease on day 18) and during lactation (18.5-fold increase on day 7). Levels appear to be minimally altered during involution.1 Publication

Gene expression databases

GenevisibleiP16612. RN.

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Also found as heterodimer with PGF. Interacts with NRP1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi249830. 1 interactor.
STRINGi10116.ENSRNOP00000026637.

Structurei

3D structure databases

ProteinModelPortaliP16612.
SMRiP16612.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PDGF/VEGF growth factor family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IGCM. Eukaryota.
ENOG410ZWYU. LUCA.
HOGENOMiHOG000072704.
HOVERGENiHBG000105.
InParanoidiP16612.
KOiK05448.
PhylomeDBiP16612.

Family and domain databases

CDDicd00135. PDGF. 1 hit.
Gene3Di2.10.160.10. 1 hit.
2.10.90.10. 1 hit.
InterProiView protein in InterPro
IPR029034. Cystine-knot_cytokine.
IPR023581. PD_growth_factor_CS.
IPR000072. PDGF/VEGF_dom.
IPR027928. VEGF_C.
IPR036841. VEGF_C_sf.
PfamiView protein in Pfam
PF00341. PDGF. 1 hit.
PF14554. VEGF_C. 1 hit.
SMARTiView protein in SMART
SM00141. PDGF. 1 hit.
SUPFAMiSSF57501. SSF57501. 1 hit.
SSF57593. SSF57593. 1 hit.
PROSITEiView protein in PROSITE
PS00249. PDGF_1. 1 hit.
PS50278. PDGF_2. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform VEGF-A188 (identifier: P16612-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNFLLSWVHW TLALLLYLHH AKWSQAAPTT EGEQKAHEVV KFMDVYQRSY
60 70 80 90 100
CRPIETLVDI FQEYPDEIEY IFKPSCVPLM RCAGCCNDEA LECVPTSESN
110 120 130 140 150
VTMQIMRIKP HQSQHIGEMS FLQHSRCECR PKKDRTKPEK KSVRGKGKGQ
160 170 180 190 200
KRKRKKSRFK SWSVHCEPCS ERRKHLFVQD PQTCKCSCKN TDSRCKARQL
210
ELNERTCRCD KPRR
Length:214
Mass (Da):25,239
Last modified:November 16, 2001 - v2
Checksum:i60FBB876F5304946
GO
Isoform VEGF-A164 (identifier: P16612-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     140-140: K → N
     141-164: Missing.

Show »
Length:190
Mass (Da):22,396
Checksum:i589374010441F377
GO
Isoform VEGF-A144 (identifier: P16612-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     165-208: Missing.

Show »
Length:170
Mass (Da):20,018
Checksum:iB371BF4A67E3231B
GO
Isoform VEGF-A120 (identifier: P16612-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     141-208: Missing.

Show »
Length:146
Mass (Da):17,189
Checksum:i8DC2979C38FCF1A0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti101V → A in AAF19212 (PubMed:11163598).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_004629140K → N in isoform VEGF-A164. 3 Publications1
Alternative sequenceiVSP_004631141 – 208Missing in isoform VEGF-A120. 1 PublicationAdd BLAST68
Alternative sequenceiVSP_004630141 – 164Missing in isoform VEGF-A164. 3 PublicationsAdd BLAST24
Alternative sequenceiVSP_004632165 – 208Missing in isoform VEGF-A144. 1 PublicationAdd BLAST44

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32167 mRNA. Translation: AAA41211.1.
AF215725 mRNA. Translation: AAF19211.1.
AF215726 mRNA. Translation: AAF19212.1.
AF222779 mRNA. Translation: AAF25958.1.
AY033506 mRNA. Translation: AAL07526.1.
AY033508 mRNA. Translation: AAL07528.1.
PIRiA35987.
RefSeqiNP_001103804.1. NM_001110334.2.
NP_001274036.1. NM_001287107.1. [P16612-1]
NP_001274037.1. NM_001287108.1. [P16612-2]
NP_001274039.1. NM_001287110.1. [P16612-4]
NP_001274040.1. NM_001287111.1.
NP_001274041.1. NM_001287112.1. [P16612-3]
NP_001274043.1. NM_001287114.1.
NP_114024.2. NM_031836.3.
UniGeneiRn.1923.

Genome annotation databases

GeneIDi83785.
KEGGirno:83785.
UCSCiRGD:619991. rat. [P16612-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiVEGFA_RAT
AccessioniPrimary (citable) accession number: P16612
Secondary accession number(s): Q541S6
, Q91ZE1, Q9JKX7, Q9QXG6, Q9QXG7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: November 16, 2001
Last modified: November 22, 2017
This is version 159 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families