P16611 (IBP3_PIG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Insulin-like growth factor-binding protein 3
Short name=IBP-3
Short name=IGF-binding protein 3
Short name=IGFBP-3

Gene names

Name:

IGFBP3

Organism

Sus scrofa (Pig) [Reference proteome]

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	293 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R.
Subunit structure	Interacts with XLKD1. Binds IGF2 more than IGF1. Forms a ternary complex of about 140 to 150 kDa with IGF1 or IGF2 and a 85 kDa glycoprotein (ALS). Interacts with HN. Interacts with TMEM219 By similarity.
Subcellular location	Secreted.
Post-translational modification	Phosphorylation sites are present in the extracellular medium By similarity.
Sequence similarities	Contains 1 IGFBP N-terminal domain. Contains 1 thyroglobulin type-1 domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Growth factor binding
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of apoptotic process Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of myoblast differentiation Inferred from sequence or structural similarity. Source: UniProtKB regulation of cell growth Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein tyrosine phosphatase activator activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Ref.4
	Chain	28 – 293	266	Insulin-like growth factor-binding protein 3		PRO_0000045904
Regions
	Domain	36 – 119	84	IGFBP N-terminal
	Domain	212 – 287	76	Thyroglobulin type-1
Amino acid modifications
	Glycosylation	118	1	N-linked (GlcNAc...) Potential
	Glycosylation	138	1	N-linked (GlcNAc...) Potential
	Glycosylation	201	1	N-linked (GlcNAc...) Potential
	Disulfide bond	215 ↔ 242		By similarity
	Disulfide bond	253 ↔ 264		By similarity
	Disulfide bond	266 ↔ 287		By similarity
Experimental info
	Sequence conflict	23	1	P → S in AAF23229. Ref.1
	Sequence conflict	32	1	A → V Ref.1
	Sequence conflict	32	1	A → V Ref.3
	Sequence conflict	170	1	V → I in AAF23229. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P16611 [UniParc]. Last modified February 7, 2006. Version 3. Checksum: B2ECED337B91DA83	FASTA	293	31,690
	10 20 30 40 50 60 MQRARPALWA AALIALALLR GPPAARAGSG AAGTGPVVRC EPCDARALAQ CAPPPAAPPC 70 80 90 100 110 120 AELVREPGCG CCLTCALREG QACGVYTERC GAGLRCQPPP GEPRPLQALL DGRGICANAS 130 140 150 160 170 180 AAGRLRAYLL PAPPAPGNGS ESEEDRSVDS MENQALPSTH RVPDSKLHSV HTKMDVIKKG 190 200 210 220 230 240 HAKDSQRYKV DYESQSTDTQ NFSSESKRET EYGPCRREME DTLNHLKFLN MLSPRGIHIP 250 260 270 280 290 NCDKKGFYKK KQCRPSKGRK RGFCWCVDKY GQPLPGFDVK GKGDVHCYSM ESK

References

[1]	"Effect of recombinant porcine IGF-binding protein-3 on proliferation of embryonic porcine myogenic cell cultures in the presence and absence of IGF-I." Pampusch M.S., Kamanga-Sollo E., White M.E., Hathaway M.R., Dayton W.R. J. Endocrinol. 176:227-235(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"Insulin-like growth factor-binding protein-3 in porcine ovarian granulosa cells: gene cloning, promoter mapping, and follicle-stimulating hormone regulation." Ongeri E.M., Zhu Q., Verderame M.F., Hammond J.M. Endocrinology 145:1776-1785(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Structural characterization of a follicle-stimulating hormone action inhibitor in porcine ovarian follicular fluid. Its identification as the insulin-like growth factor-binding protein." Shimasaki S., Shimonaka M., Ui M., Inouye S., Shibata F., Ling N. J. Biol. Chem. 265:2198-2202(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 28-293.
[4]	"Identification and NH2-terminal amino acid sequence of three insulin-like growth factor-binding proteins in porcine serum." Coleman M.E., Pan Y.-C.E., Etherton T.D. Biochem. Biophys. Res. Commun. 181:1131-1136(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 28-42.
[5]	"Study on SNPs of porcine IGFBP-3 gene." Liu D., Zhang Y., Zhang X., Yang G. Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 143-293.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF085482 mRNA. Translation: AAF23229.1. AY464121 Genomic DNA. Translation: AAR87008.1. J05228 mRNA. Translation: AAA31054.1. AY422045 Genomic DNA. Translation: AAQ97624.1.
PIR	A35037. JH0516.
RefSeq	NP_001005156.1. NM_001005156.1.
UniGene	Ssc.42774.
3D structure databases
ProteinModelPortal	P16611.
SMR	P16611. Positions 38-118.
ModBase	Search...
Protein family/group databases
MEROPS	I31.952.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	448812.
KEGG	ssc:448812.
Organism-specific databases
CTD	3486.
Phylogenomic databases
HOVERGEN	HBG002631.
KO	K10138.
Family and domain databases
Gene3D	4.10.800.10. 1 hit.
InterPro	IPR009030. Growth_fac_rcpt. IPR012211. IGFBP-3. IPR000867. IGFBP-like. IPR009168. IGFBP1-6. IPR022321. IGFBP_1-6_chordata. IPR017891. Insulin_GF-bd_Cys-rich_CS. IPR000716. Thyroglobulin_1. [Graphical view]
PANTHER	PTHR11551. PTHR11551. 1 hit. PTHR11551:SF3. PTHR11551:SF3. 1 hit.
Pfam	PF00219. IGFBP. 1 hit. PF00086. Thyroglobulin_1. 1 hit. [Graphical view]
PRINTS	PR01976. IGFBPFAMILY. PR01979. IGFBPFAMILY3.
SMART	SM00121. IB. 1 hit. SM00211. TY. 1 hit. [Graphical view]
SUPFAM	SSF57184. Grow_fac_recept. 1 hit. SSF57610. Thyroglobulin_1. 1 hit.
PROSITE	PS00222. IGFBP_N_1. 1 hit. PS51323. IGFBP_N_2. 1 hit. PS00484. THYROGLOBULIN_1_1. 1 hit. PS51162. THYROGLOBULIN_1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IBP3_PIG

Accession

Primary (citable) accession number: P16611
Secondary accession number(s): Q6S6K2, Q9TTI0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	February 7, 2006
Last modified:	April 3, 2013
This is version 108 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program