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P16611 (IBP3_PIG) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Insulin-like growth factor-binding protein 3

Short name=IBP-3
Short name=IGF-binding protein 3
Short name=IGFBP-3
Gene names
Name:IGFBP3
OrganismSus scrofa (Pig) [Reference proteome]
Taxonomic identifier9823 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus

Protein attributes

Sequence length293 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R.

Subunit structure

Interacts with XLKD1. Binds IGF2 more than IGF1. Forms a ternary complex of about 140 to 150 kDa with IGF1 or IGF2 and a 85 kDa glycoprotein (ALS). Interacts with HN. Interacts with TMEM219 By similarity.

Subcellular location

Secreted.

Post-translational modification

Phosphorylation sites are present in the extracellular medium By similarity.

Sequence similarities

Contains 1 IGFBP N-terminal domain.

Contains 1 thyroglobulin type-1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Ref.4
Chain28 – 293266Insulin-like growth factor-binding protein 3
PRO_0000045904

Regions

Domain36 – 11984IGFBP N-terminal
Domain212 – 28776Thyroglobulin type-1

Amino acid modifications

Glycosylation1181N-linked (GlcNAc...) Potential
Glycosylation1381N-linked (GlcNAc...) Potential
Glycosylation2011N-linked (GlcNAc...) Potential
Disulfide bond215 ↔ 242 By similarity
Disulfide bond253 ↔ 264 By similarity
Disulfide bond266 ↔ 287 By similarity

Experimental info

Sequence conflict231P → S in AAF23229. Ref.1
Sequence conflict321A → V Ref.1
Sequence conflict321A → V Ref.3
Sequence conflict1701V → I in AAF23229. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P16611 [UniParc].

Last modified February 7, 2006. Version 3.
Checksum: B2ECED337B91DA83

FASTA29331,690
        10         20         30         40         50         60 
MQRARPALWA AALIALALLR GPPAARAGSG AAGTGPVVRC EPCDARALAQ CAPPPAAPPC 

        70         80         90        100        110        120 
AELVREPGCG CCLTCALREG QACGVYTERC GAGLRCQPPP GEPRPLQALL DGRGICANAS 

       130        140        150        160        170        180 
AAGRLRAYLL PAPPAPGNGS ESEEDRSVDS MENQALPSTH RVPDSKLHSV HTKMDVIKKG 

       190        200        210        220        230        240 
HAKDSQRYKV DYESQSTDTQ NFSSESKRET EYGPCRREME DTLNHLKFLN MLSPRGIHIP 

       250        260        270        280        290 
NCDKKGFYKK KQCRPSKGRK RGFCWCVDKY GQPLPGFDVK GKGDVHCYSM ESK 

« Hide

References

[1]"Effect of recombinant porcine IGF-binding protein-3 on proliferation of embryonic porcine myogenic cell cultures in the presence and absence of IGF-I."
Pampusch M.S., Kamanga-Sollo E., White M.E., Hathaway M.R., Dayton W.R.
J. Endocrinol. 176:227-235(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[2]"Insulin-like growth factor-binding protein-3 in porcine ovarian granulosa cells: gene cloning, promoter mapping, and follicle-stimulating hormone regulation."
Ongeri E.M., Zhu Q., Verderame M.F., Hammond J.M.
Endocrinology 145:1776-1785(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Structural characterization of a follicle-stimulating hormone action inhibitor in porcine ovarian follicular fluid. Its identification as the insulin-like growth factor-binding protein."
Shimasaki S., Shimonaka M., Ui M., Inouye S., Shibata F., Ling N.
J. Biol. Chem. 265:2198-2202(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 28-293.
[4]"Identification and NH2-terminal amino acid sequence of three insulin-like growth factor-binding proteins in porcine serum."
Coleman M.E., Pan Y.-C.E., Etherton T.D.
Biochem. Biophys. Res. Commun. 181:1131-1136(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 28-42.
[5]"Study on SNPs of porcine IGFBP-3 gene."
Liu D., Zhang Y., Zhang X., Yang G.
Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 143-293.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF085482 mRNA. Translation: AAF23229.1.
AY464121 Genomic DNA. Translation: AAR87008.1.
J05228 mRNA. Translation: AAA31054.1.
AY422045 Genomic DNA. Translation: AAQ97624.1.
PIRA35037.
JH0516.
RefSeqNP_001005156.1. NM_001005156.1.
UniGeneSsc.42774.

3D structure databases

ProteinModelPortalP16611.
SMRP16611. Positions 38-118.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSI31.952.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID448812.
KEGGssc:448812.

Organism-specific databases

CTD3486.

Phylogenomic databases

HOVERGENHBG002631.
KOK10138.

Family and domain databases

Gene3D4.10.40.20. 1 hit.
4.10.800.10. 1 hit.
InterProIPR009030. Growth_fac_rcpt_N_dom.
IPR012211. IGFBP-3.
IPR000867. IGFBP-like.
IPR009168. IGFBP1-6.
IPR022321. IGFBP_1-6_chordata.
IPR017891. Insulin_GF-bd_Cys-rich_CS.
IPR000716. Thyroglobulin_1.
[Graphical view]
PANTHERPTHR11551. PTHR11551. 1 hit.
PTHR11551:SF3. PTHR11551:SF3. 1 hit.
PfamPF00219. IGFBP. 1 hit.
PF00086. Thyroglobulin_1. 1 hit.
[Graphical view]
PRINTSPR01976. IGFBPFAMILY.
PR01979. IGFBPFAMILY3.
SMARTSM00121. IB. 1 hit.
SM00211. TY. 1 hit.
[Graphical view]
SUPFAMSSF57184. SSF57184. 1 hit.
SSF57610. SSF57610. 1 hit.
PROSITEPS00222. IGFBP_N_1. 1 hit.
PS51323. IGFBP_N_2. 1 hit.
PS00484. THYROGLOBULIN_1_1. 1 hit.
PS51162. THYROGLOBULIN_1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameIBP3_PIG
AccessionPrimary (citable) accession number: P16611
Secondary accession number(s): Q6S6K2, Q9TTI0
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: February 7, 2006
Last modified: April 16, 2014
This is version 111 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families