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Protein

Insulin-like growth factor-binding protein 3

Gene

IGFBP3

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R.

GO - Molecular functioni

  1. protein tyrosine phosphatase activator activity Source: UniProtKB

GO - Biological processi

  1. positive regulation of apoptotic process Source: UniProtKB
  2. positive regulation of catalytic activity Source: GOC
  3. positive regulation of myoblast differentiation Source: UniProtKB
  4. regulation of cell growth Source: InterPro
Complete GO annotation...

Keywords - Ligandi

Growth factor binding

Protein family/group databases

MEROPSiI31.952.

Names & Taxonomyi

Protein namesi
Recommended name:
Insulin-like growth factor-binding protein 3
Short name:
IBP-3
Short name:
IGF-binding protein 3
Short name:
IGFBP-3
Gene namesi
Name:IGFBP3
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 27271 PublicationAdd
BLAST
Chaini28 – 293266Insulin-like growth factor-binding protein 3PRO_0000045904Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi118 – 1181N-linked (GlcNAc...)Sequence Analysis
Glycosylationi138 – 1381N-linked (GlcNAc...)Sequence Analysis
Glycosylationi201 – 2011N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi215 ↔ 242PROSITE-ProRule annotation
Disulfide bondi253 ↔ 264PROSITE-ProRule annotation
Disulfide bondi266 ↔ 287PROSITE-ProRule annotation

Post-translational modificationi

Phosphorylation sites are present in the extracellular medium.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Interactioni

Subunit structurei

Interacts with XLKD1. Binds IGF2 more than IGF1. Forms a ternary complex of about 140 to 150 kDa with IGF1 or IGF2 and a 85 kDa glycoprotein (ALS). Interacts with HN. Interacts with TMEM219 (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP16611.
SMRiP16611. Positions 38-118.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini36 – 11984IGFBP N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini212 – 28776Thyroglobulin type-1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 IGFBP N-terminal domain.PROSITE-ProRule annotation
Contains 1 thyroglobulin type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG002631.
InParanoidiP16611.
KOiK10138.

Family and domain databases

Gene3Di4.10.40.20. 1 hit.
4.10.800.10. 1 hit.
InterProiIPR009030. Growth_fac_rcpt_N_dom.
IPR012211. IGFBP-3.
IPR000867. IGFBP-like.
IPR009168. IGFBP1-6.
IPR022321. IGFBP_1-6_chordata.
IPR017891. Insulin_GF-bd_Cys-rich_CS.
IPR000716. Thyroglobulin_1.
[Graphical view]
PANTHERiPTHR11551. PTHR11551. 1 hit.
PTHR11551:SF3. PTHR11551:SF3. 1 hit.
PfamiPF00219. IGFBP. 1 hit.
PF00086. Thyroglobulin_1. 1 hit.
[Graphical view]
PRINTSiPR01976. IGFBPFAMILY.
PR01979. IGFBPFAMILY3.
SMARTiSM00121. IB. 1 hit.
SM00211. TY. 1 hit.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 1 hit.
SSF57610. SSF57610. 1 hit.
PROSITEiPS00222. IGFBP_N_1. 1 hit.
PS51323. IGFBP_N_2. 1 hit.
PS00484. THYROGLOBULIN_1_1. 1 hit.
PS51162. THYROGLOBULIN_1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P16611-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQRARPALWA AALIALALLR GPPAARAGSG AAGTGPVVRC EPCDARALAQ
60 70 80 90 100
CAPPPAAPPC AELVREPGCG CCLTCALREG QACGVYTERC GAGLRCQPPP
110 120 130 140 150
GEPRPLQALL DGRGICANAS AAGRLRAYLL PAPPAPGNGS ESEEDRSVDS
160 170 180 190 200
MENQALPSTH RVPDSKLHSV HTKMDVIKKG HAKDSQRYKV DYESQSTDTQ
210 220 230 240 250
NFSSESKRET EYGPCRREME DTLNHLKFLN MLSPRGIHIP NCDKKGFYKK
260 270 280 290
KQCRPSKGRK RGFCWCVDKY GQPLPGFDVK GKGDVHCYSM ESK
Length:293
Mass (Da):31,690
Last modified:February 7, 2006 - v3
Checksum:iB2ECED337B91DA83
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti23 – 231P → S in AAF23229. (PubMed:12553871)Curated
Sequence conflicti32 – 321A → V(PubMed:12553871)Curated
Sequence conflicti32 – 321A → V(PubMed:1688850)Curated
Sequence conflicti170 – 1701V → I in AAF23229. (PubMed:12553871)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF085482 mRNA. Translation: AAF23229.1.
AY464121 Genomic DNA. Translation: AAR87008.1.
J05228 mRNA. Translation: AAA31054.1.
AY422045 Genomic DNA. Translation: AAQ97624.1.
PIRiA35037.
JH0516.
RefSeqiNP_001005156.1. NM_001005156.1.
UniGeneiSsc.42774.

Genome annotation databases

GeneIDi448812.
KEGGissc:448812.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF085482 mRNA. Translation: AAF23229.1.
AY464121 Genomic DNA. Translation: AAR87008.1.
J05228 mRNA. Translation: AAA31054.1.
AY422045 Genomic DNA. Translation: AAQ97624.1.
PIRiA35037.
JH0516.
RefSeqiNP_001005156.1. NM_001005156.1.
UniGeneiSsc.42774.

3D structure databases

ProteinModelPortaliP16611.
SMRiP16611. Positions 38-118.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiI31.952.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi448812.
KEGGissc:448812.

Organism-specific databases

CTDi3486.

Phylogenomic databases

HOVERGENiHBG002631.
InParanoidiP16611.
KOiK10138.

Family and domain databases

Gene3Di4.10.40.20. 1 hit.
4.10.800.10. 1 hit.
InterProiIPR009030. Growth_fac_rcpt_N_dom.
IPR012211. IGFBP-3.
IPR000867. IGFBP-like.
IPR009168. IGFBP1-6.
IPR022321. IGFBP_1-6_chordata.
IPR017891. Insulin_GF-bd_Cys-rich_CS.
IPR000716. Thyroglobulin_1.
[Graphical view]
PANTHERiPTHR11551. PTHR11551. 1 hit.
PTHR11551:SF3. PTHR11551:SF3. 1 hit.
PfamiPF00219. IGFBP. 1 hit.
PF00086. Thyroglobulin_1. 1 hit.
[Graphical view]
PRINTSiPR01976. IGFBPFAMILY.
PR01979. IGFBPFAMILY3.
SMARTiSM00121. IB. 1 hit.
SM00211. TY. 1 hit.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 1 hit.
SSF57610. SSF57610. 1 hit.
PROSITEiPS00222. IGFBP_N_1. 1 hit.
PS51323. IGFBP_N_2. 1 hit.
PS00484. THYROGLOBULIN_1_1. 1 hit.
PS51162. THYROGLOBULIN_1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Effect of recombinant porcine IGF-binding protein-3 on proliferation of embryonic porcine myogenic cell cultures in the presence and absence of IGF-I."
    Pampusch M.S., Kamanga-Sollo E., White M.E., Hathaway M.R., Dayton W.R.
    J. Endocrinol. 176:227-235(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. "Insulin-like growth factor-binding protein-3 in porcine ovarian granulosa cells: gene cloning, promoter mapping, and follicle-stimulating hormone regulation."
    Ongeri E.M., Zhu Q., Verderame M.F., Hammond J.M.
    Endocrinology 145:1776-1785(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Structural characterization of a follicle-stimulating hormone action inhibitor in porcine ovarian follicular fluid. Its identification as the insulin-like growth factor-binding protein."
    Shimasaki S., Shimonaka M., Ui M., Inouye S., Shibata F., Ling N.
    J. Biol. Chem. 265:2198-2202(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 28-293.
  4. "Identification and NH2-terminal amino acid sequence of three insulin-like growth factor-binding proteins in porcine serum."
    Coleman M.E., Pan Y.-C.E., Etherton T.D.
    Biochem. Biophys. Res. Commun. 181:1131-1136(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 28-42.
  5. "Study on SNPs of porcine IGFBP-3 gene."
    Liu D., Zhang Y., Zhang X., Yang G.
    Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 143-293.

Entry informationi

Entry nameiIBP3_PIG
AccessioniPrimary (citable) accession number: P16611
Secondary accession number(s): Q6S6K2, Q9TTI0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: February 7, 2006
Last modified: January 7, 2015
This is version 114 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.