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Protein

Tryptophan synthase alpha chain

Gene

trpA

Organism
Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate.UniRule annotation

Catalytic activityi

L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O.UniRule annotation

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes L-tryptophan from chorismate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Anthranilate phosphoribosyltransferase (trpD)
  3. N-(5'-phosphoribosyl)anthranilate isomerase (trpF)
  4. Indole-3-glycerol phosphate synthase (TT_C0800)
  5. Tryptophan synthase beta chain (trpB), Tryptophan synthase alpha chain (trpA)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei47 – 471Proton acceptorUniRule annotation
Active sitei58 – 581Proton acceptorUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

BioCyciTTHE262724:GCAT-743-MONOMER.
BRENDAi4.2.1.20. 2305.
UniPathwayiUPA00035; UER00044.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan synthase alpha chainUniRule annotation (EC:4.2.1.20UniRule annotation)
Gene namesi
Name:trpAUniRule annotation
Ordered Locus Names:TT_C0729
OrganismiThermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
Taxonomic identifieri262724 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000592 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 271271Tryptophan synthase alpha chainPRO_0000098865Add
BLAST

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains.UniRule annotation

Protein-protein interaction databases

STRINGi262724.TTC0729.

Structurei

Secondary structure

1
271
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 1210Combined sources
Beta strandi17 – 237Combined sources
Helixi29 – 3911Combined sources
Helixi40 – 423Combined sources
Beta strandi44 – 496Combined sources
Helixi60 – 7112Combined sources
Helixi76 – 8914Combined sources
Beta strandi94 – 974Combined sources
Helixi100 – 1067Combined sources
Helixi108 – 11811Combined sources
Beta strandi122 – 1243Combined sources
Helixi130 – 1323Combined sources
Helixi134 – 14310Combined sources
Beta strandi146 – 1483Combined sources
Helixi157 – 1648Combined sources
Beta strandi171 – 1744Combined sources
Helixi191 – 1988Combined sources
Beta strandi205 – 2095Combined sources
Helixi214 – 2207Combined sources
Beta strandi223 – 2286Combined sources
Helixi230 – 2378Combined sources
Helixi242 – 25312Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1UJPX-ray1.34A1-270[»]
1WXJX-ray1.70A1-270[»]
ProteinModelPortaliP16608.
SMRiP16608. Positions 1-256.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP16608.

Family & Domainsi

Sequence similaritiesi

Belongs to the TrpA family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105F6H. Bacteria.
COG0159. LUCA.
KOiK01695.
OMAiPVFICPP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00131. Trp_synth_alpha. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR011060. RibuloseP-bd_barrel.
IPR018204. Trp_synthase_alpha_AS.
IPR002028. Trp_synthase_suA.
[Graphical view]
PfamiPF00290. Trp_syntA. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR00262. trpA. 1 hit.
PROSITEiPS00167. TRP_SYNTHASE_ALPHA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P16608-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTLEAFAKA RSEGRAALIP YLTAGFPSRE GFLQAVEEVL PYADLLEIGL
60 70 80 90 100
PYSDPLGDGP VIQRASELAL RKGMSVQGAL ELVREVRALT EKPLFLMTYL
110 120 130 140 150
NPVLAWGPER FFGLFKQAGA TGVILPDLPP DEDPGLVRLA QEIGLETVFL
160 170 180 190 200
LAPTSTDARI ATVVRHATGF VYAVSVTGVT GMRERLPEEV KDLVRRIKAR
210 220 230 240 250
TALPVAVGFG VSGKATAAQA AVADGVVVGS ALVRALEEGR SLAPLLQEIR
260 270
QGLQRLEANP GLKESSKKPL S
Length:271
Mass (Da):28,937
Last modified:May 24, 2004 - v2
Checksum:i88A7B82187223AA5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti171 – 1711V → S in AAA27509 (PubMed:2188962).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32108 Genomic DNA. Translation: AAA27509.1.
AE017221 Genomic DNA. Translation: AAS81077.1.
PIRiB35407.
RefSeqiWP_011173168.1. NC_005835.1.

Genome annotation databases

EnsemblBacteriaiAAS81077; AAS81077; TT_C0729.
KEGGitth:TTC0729.
PATRICi23951863. VBITheThe54392_0726.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32108 Genomic DNA. Translation: AAA27509.1.
AE017221 Genomic DNA. Translation: AAS81077.1.
PIRiB35407.
RefSeqiWP_011173168.1. NC_005835.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1UJPX-ray1.34A1-270[»]
1WXJX-ray1.70A1-270[»]
ProteinModelPortaliP16608.
SMRiP16608. Positions 1-256.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi262724.TTC0729.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAS81077; AAS81077; TT_C0729.
KEGGitth:TTC0729.
PATRICi23951863. VBITheThe54392_0726.

Phylogenomic databases

eggNOGiENOG4105F6H. Bacteria.
COG0159. LUCA.
KOiK01695.
OMAiPVFICPP.

Enzyme and pathway databases

UniPathwayiUPA00035; UER00044.
BioCyciTTHE262724:GCAT-743-MONOMER.
BRENDAi4.2.1.20. 2305.

Miscellaneous databases

EvolutionaryTraceiP16608.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00131. Trp_synth_alpha. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR011060. RibuloseP-bd_barrel.
IPR018204. Trp_synthase_alpha_AS.
IPR002028. Trp_synthase_suA.
[Graphical view]
PfamiPF00290. Trp_syntA. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR00262. trpA. 1 hit.
PROSITEiPS00167. TRP_SYNTHASE_ALPHA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRPA_THET2
AccessioniPrimary (citable) accession number: P16608
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: May 24, 2004
Last modified: September 7, 2016
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.