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Protein

Tryptophan synthase alpha chain

Gene

trpA

Organism
Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate.UniRule annotation

Catalytic activityi

L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O.UniRule annotation

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes L-tryptophan from chorismate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Anthranilate phosphoribosyltransferase (trpD)
  3. N-(5'-phosphoribosyl)anthranilate isomerase (trpF)
  4. Indole-3-glycerol phosphate synthase (TT_C0800)
  5. Tryptophan synthase beta chain (trpB), Tryptophan synthase alpha chain (trpA)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei47Proton acceptorUniRule annotation1
Active sitei58Proton acceptorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

BRENDAi4.2.1.20. 2305.
UniPathwayiUPA00035; UER00044.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan synthase alpha chainUniRule annotation (EC:4.2.1.20UniRule annotation)
Gene namesi
Name:trpAUniRule annotation
Ordered Locus Names:TT_C0729
OrganismiThermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
Taxonomic identifieri262724 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000592 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000988651 – 271Tryptophan synthase alpha chainAdd BLAST271

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains.UniRule annotation

Protein-protein interaction databases

STRINGi262724.TTC0729.

Structurei

Secondary structure

1271
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 12Combined sources10
Beta strandi17 – 23Combined sources7
Helixi29 – 39Combined sources11
Helixi40 – 42Combined sources3
Beta strandi44 – 49Combined sources6
Helixi60 – 71Combined sources12
Helixi76 – 89Combined sources14
Beta strandi94 – 97Combined sources4
Helixi100 – 106Combined sources7
Helixi108 – 118Combined sources11
Beta strandi122 – 124Combined sources3
Helixi130 – 132Combined sources3
Helixi134 – 143Combined sources10
Beta strandi146 – 148Combined sources3
Helixi157 – 164Combined sources8
Beta strandi171 – 174Combined sources4
Helixi191 – 198Combined sources8
Beta strandi205 – 209Combined sources5
Helixi214 – 220Combined sources7
Beta strandi223 – 228Combined sources6
Helixi230 – 237Combined sources8
Helixi242 – 253Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UJPX-ray1.34A1-270[»]
1WXJX-ray1.70A1-270[»]
ProteinModelPortaliP16608.
SMRiP16608.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP16608.

Family & Domainsi

Sequence similaritiesi

Belongs to the TrpA family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105F6H. Bacteria.
COG0159. LUCA.
KOiK01695.
OMAiPVFICPP.

Family and domain databases

CDDicd04724. Tryptophan_synthase_alpha. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00131. Trp_synth_alpha. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR011060. RibuloseP-bd_barrel.
IPR018204. Trp_synthase_alpha_AS.
IPR002028. Trp_synthase_suA.
[Graphical view]
PfamiPF00290. Trp_syntA. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR00262. trpA. 1 hit.
PROSITEiPS00167. TRP_SYNTHASE_ALPHA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P16608-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTLEAFAKA RSEGRAALIP YLTAGFPSRE GFLQAVEEVL PYADLLEIGL
60 70 80 90 100
PYSDPLGDGP VIQRASELAL RKGMSVQGAL ELVREVRALT EKPLFLMTYL
110 120 130 140 150
NPVLAWGPER FFGLFKQAGA TGVILPDLPP DEDPGLVRLA QEIGLETVFL
160 170 180 190 200
LAPTSTDARI ATVVRHATGF VYAVSVTGVT GMRERLPEEV KDLVRRIKAR
210 220 230 240 250
TALPVAVGFG VSGKATAAQA AVADGVVVGS ALVRALEEGR SLAPLLQEIR
260 270
QGLQRLEANP GLKESSKKPL S
Length:271
Mass (Da):28,937
Last modified:May 24, 2004 - v2
Checksum:i88A7B82187223AA5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti171V → S in AAA27509 (PubMed:2188962).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32108 Genomic DNA. Translation: AAA27509.1.
AE017221 Genomic DNA. Translation: AAS81077.1.
PIRiB35407.
RefSeqiWP_011173168.1. NC_005835.1.

Genome annotation databases

EnsemblBacteriaiAAS81077; AAS81077; TT_C0729.
KEGGitth:TT_C0729.
PATRICi23951863. VBITheThe54392_0726.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32108 Genomic DNA. Translation: AAA27509.1.
AE017221 Genomic DNA. Translation: AAS81077.1.
PIRiB35407.
RefSeqiWP_011173168.1. NC_005835.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UJPX-ray1.34A1-270[»]
1WXJX-ray1.70A1-270[»]
ProteinModelPortaliP16608.
SMRiP16608.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi262724.TTC0729.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAS81077; AAS81077; TT_C0729.
KEGGitth:TT_C0729.
PATRICi23951863. VBITheThe54392_0726.

Phylogenomic databases

eggNOGiENOG4105F6H. Bacteria.
COG0159. LUCA.
KOiK01695.
OMAiPVFICPP.

Enzyme and pathway databases

UniPathwayiUPA00035; UER00044.
BRENDAi4.2.1.20. 2305.

Miscellaneous databases

EvolutionaryTraceiP16608.

Family and domain databases

CDDicd04724. Tryptophan_synthase_alpha. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00131. Trp_synth_alpha. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR011060. RibuloseP-bd_barrel.
IPR018204. Trp_synthase_alpha_AS.
IPR002028. Trp_synthase_suA.
[Graphical view]
PfamiPF00290. Trp_syntA. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR00262. trpA. 1 hit.
PROSITEiPS00167. TRP_SYNTHASE_ALPHA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRPA_THET2
AccessioniPrimary (citable) accession number: P16608
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: May 24, 2004
Last modified: November 2, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.