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P16586 (PDE6C_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cone cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha'

EC=3.1.4.35
Alternative name(s):
PDE V-C1
cGMP phosphodiesterase 6C
Gene names
Name:PDE6C
Synonyms:PDEA2
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length855 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.

Cofactor

Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.

Subunit structure

Composed of two alpha' subunits that are associated with 3 smaller proteins of 11, 13, and 15 kDa.

Subcellular location

Cell membrane; Lipid-anchor; Cytoplasmic side Potential.

Sequence similarities

Belongs to the cyclic nucleotide phosphodiesterase family.

Contains 2 GAF domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 852852Cone cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha'
PRO_0000198830
Propeptide853 – 8553Removed in mature form Potential
PRO_0000370787

Regions

Domain70 – 219150GAF 1
Domain251 – 428178GAF 2
Nucleotide binding164 – 1674cGMP By similarity

Sites

Active site5571Proton donor By similarity
Metal binding5611Divalent metal cation 1 By similarity
Metal binding5971Divalent metal cation 1 By similarity
Metal binding5981Divalent metal cation 1 By similarity
Metal binding5981Divalent metal cation 2 By similarity
Metal binding7181Divalent metal cation 1 By similarity
Binding site921cGMP By similarity
Binding site1161cGMP By similarity
Binding site1711cGMP By similarity

Amino acid modifications

Modified residue8521Cysteine methyl ester Potential
Lipidation8521S-geranylgeranyl cysteine By similarity

Sequences

Sequence LengthMass (Da)Tools
P16586 [UniParc].

Last modified August 1, 1990. Version 1.
Checksum: 1FCFFFD045686D65

FASTA85598,798
        10         20         30         40         50         60 
MGEISQETVE KYLEANPQFA KEYFNRKLQV EVPSGGAQAP ASASFPGRTL AEEAALYLEL 

        70         80         90        100        110        120 
LEVLLEEAGS VELAAHRALQ RLAQLLQADR CSMFLCRARN GTPEVASKLL DVTPTSKFED 

       130        140        150        160        170        180 
NLVVPDREAV FPLDVGIVGW VAHTKKTFNV PDVKKNSHFS DFMDKQTGYV TRNLLATPIV 

       190        200        210        220        230        240 
MGKEVLAVFM AVNKVDASEF SKQDEEVFSK YLSFVSIILK LHHTNYLYNI ESRRSQILMW 

       250        260        270        280        290        300 
SANKVFEELT DVERQFHKAL YTVRTYLNCE RYSIGLLDMT KEKEFYDEWP VKLGEVEPYK 

       310        320        330        340        350        360 
GPKTPDGREV IFYKIIDYIL HGKEEIKVIP TPPMDHWTLI SGLPTYVAEN GFICNMLNAP 

       370        380        390        400        410        420 
ADEYFTFQKG PVDETGWVIK NVLSLPIVNK KEDIVGVATF YNRKDGKPFD EYDEHIAETL 

       430        440        450        460        470        480 
TQFLGWSLLN TDTYEKMNKL ENRKDIAQEM LMNHTKATPD EIKSILKFKE KLNIDVIEDC 

       490        500        510        520        530        540 
EEKQLVTILK EDLPDPRTAD LYEFRFRHLP ITEHELIKCG LRLFFEINVV EKFKVPVEVL 

       550        560        570        580        590        600 
TRWMYTVRKG YRAVTYHNWR HGFNVGQTMF TLLMTGRLKK YYTDLEAFAM LAAAFCHDID 

       610        620        630        640        650        660 
HRGTNNLYQM KSTSPLARLH GSSILERHHL EYSKTLLQDE SLNIFQNLNK RQYETVIHLF 

       670        680        690        700        710        720 
EVAIIATDLA LYFKKRTMFQ KIVDACEKME TEEEAIKYVT IDPTKKEIIM AMMMTACDLS 

       730        740        750        760        770        780 
AITKPWEVQS QVALLVANEF WEQGDLERTV LQQQPIPMMD RNKKDELPKL QVGFIDFVCT 

       790        800        810        820        830        840 
FVYKEFSRFH KEITPMLNGL QNNRVEWKSL ADEYDEKMKV IEEMKKQEEG NTTEKAVEDS 

       850 
GGGGDDKKSK TCLML 

« Hide

References

[1]"Bovine cone photoreceptor cGMP phosphodiesterase structure deduced from a cDNA clone."
Li T., Volpp K., Applebury M.L.
Proc. Natl. Acad. Sci. U.S.A. 87:293-297(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Identification of a noncatalytic cGMP-binding domain conserved in both the cGMP-stimulated and photoreceptor cyclic nucleotide phosphodiesterases."
Charbonneau H., Prusti R.K., Letrong H., Sonnenburg W.K., Mullaney P.J., Walsh K., Beavo J.A.
Proc. Natl. Acad. Sci. U.S.A. 87:288-292(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 308-502, PARTIAL PROTEIN SEQUENCE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M37838 mRNA. Translation: AAA30687.1.
M33140 mRNA. Translation: AAA30688.1.
PIRA34810.
RefSeqNP_776844.1. NM_174419.1.
UniGeneBt.444.

3D structure databases

ProteinModelPortalP16586.
SMRP16586. Positions 52-220.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000049725.

Chemistry

BindingDBP16586.
ChEMBLCHEMBL2096979.

Proteomic databases

PRIDEP16586.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID281975.
KEGGbta:281975.

Organism-specific databases

CTD5146.

Phylogenomic databases

eggNOGNOG290744.
HOGENOMHOG000007069.
HOVERGENHBG053539.
KOK13757.

Family and domain databases

Gene3D1.10.1300.10. 1 hit.
3.30.450.40. 3 hits.
InterProIPR003018. GAF.
IPR029016. GAF_dom_like.
IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamPF01590. GAF. 2 hits.
PF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSPR00387. PDIESTERASE1.
SMARTSM00065. GAF. 2 hits.
SM00471. HDc. 1 hit.
[Graphical view]
SUPFAMSSF55781. SSF55781. 3 hits.
PROSITEPS00126. PDEASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20805846.

Entry information

Entry namePDE6C_BOVIN
AccessionPrimary (citable) accession number: P16586
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: June 11, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families