Reviewed, UniProtKB/Swiss-Prot P16586 (PDE6C_BOVIN)

Names and origin

Protein names

Recommended name:
    Cone cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha'
    EC=3.1.4.35
Alternative name(s):
    cGMP phosphodiesterase 6C
    PDE V-C1

Gene names

Name:	PDE6C
Synonyms:	PDEA2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	855 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.
Subunit structure	Composed of two alpha' subunits that are associated with 3 smaller proteins of 11, 13, and 15 kDa.
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side Potential.
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family. Contains 2 GAF domains.

Ontologies

Keywords
Biological process	Sensory transduction Vision
Cellular component	Cell membrane Membrane
Domain	Repeat
Ligand	cGMP
Molecular function	Hydrolase
PTM	Lipoprotein Methylation Prenylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	internal side of plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3',5'-cyclic-GMP phosphodiesterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 852	852	Cone cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha'		PRO_0000198830
	Propeptide	853 – 855	3	Removed in mature form Potential		PRO_0000370787
Regions
	Domain	70 – 219	150	GAF 1
	Domain	251 – 428	178	GAF 2
Amino acid modifications
	Modified residue	852	1	Cysteine methyl ester Potential
	Lipidation	852	1	S-geranylgeranyl cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P16586-1 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: 1FCFFFD045686D65	FASTA	855	98,798
	10 20 30 40 50 60 MGEISQETVE KYLEANPQFA KEYFNRKLQV EVPSGGAQAP ASASFPGRTL AEEAALYLEL 70 80 90 100 110 120 LEVLLEEAGS VELAAHRALQ RLAQLLQADR CSMFLCRARN GTPEVASKLL DVTPTSKFED 130 140 150 160 170 180 NLVVPDREAV FPLDVGIVGW VAHTKKTFNV PDVKKNSHFS DFMDKQTGYV TRNLLATPIV 190 200 210 220 230 240 MGKEVLAVFM AVNKVDASEF SKQDEEVFSK YLSFVSIILK LHHTNYLYNI ESRRSQILMW 250 260 270 280 290 300 SANKVFEELT DVERQFHKAL YTVRTYLNCE RYSIGLLDMT KEKEFYDEWP VKLGEVEPYK 310 320 330 340 350 360 GPKTPDGREV IFYKIIDYIL HGKEEIKVIP TPPMDHWTLI SGLPTYVAEN GFICNMLNAP 370 380 390 400 410 420 ADEYFTFQKG PVDETGWVIK NVLSLPIVNK KEDIVGVATF YNRKDGKPFD EYDEHIAETL 430 440 450 460 470 480 TQFLGWSLLN TDTYEKMNKL ENRKDIAQEM LMNHTKATPD EIKSILKFKE KLNIDVIEDC 490 500 510 520 530 540 EEKQLVTILK EDLPDPRTAD LYEFRFRHLP ITEHELIKCG LRLFFEINVV EKFKVPVEVL 550 560 570 580 590 600 TRWMYTVRKG YRAVTYHNWR HGFNVGQTMF TLLMTGRLKK YYTDLEAFAM LAAAFCHDID 610 620 630 640 650 660 HRGTNNLYQM KSTSPLARLH GSSILERHHL EYSKTLLQDE SLNIFQNLNK RQYETVIHLF 670 680 690 700 710 720 EVAIIATDLA LYFKKRTMFQ KIVDACEKME TEEEAIKYVT IDPTKKEIIM AMMMTACDLS 730 740 750 760 770 780 AITKPWEVQS QVALLVANEF WEQGDLERTV LQQQPIPMMD RNKKDELPKL QVGFIDFVCT 790 800 810 820 830 840 FVYKEFSRFH KEITPMLNGL QNNRVEWKSL ADEYDEKMKV IEEMKKQEEG NTTEKAVEDS 850 GGGGDDKKSK TCLML

References

[1]	"Bovine cone photoreceptor cGMP phosphodiesterase structure deduced from a cDNA clone." Li T., Volpp K., Applebury M.L. Proc. Natl. Acad. Sci. U.S.A. 87:293-297(1990) [PubMed: 2153291] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Identification of a noncatalytic cGMP-binding domain conserved in both the cGMP-stimulated and photoreceptor cyclic nucleotide phosphodiesterases." Charbonneau H., Prusti R.K., Letrong H., Sonnenburg W.K., Mullaney P.J., Walsh K., Beavo J.A. Proc. Natl. Acad. Sci. U.S.A. 87:288-292(1990) [PubMed: 2153290] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 308-502, PARTIAL PROTEIN SEQUENCE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M37838 mRNA. Translation: AAA30687.1. M33140 mRNA. Translation: AAA30688.1.
IPI	IPI00907998.
PIR	A34810.
RefSeq	NP_776844.1.
UniGene	Bt.444
3D structure databases
HSSP	HSSP built from PDB template 1OYN based on UniProtKB Q08499.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000055943; ENSBTAP00000048045; ENSBTAG00000000445; Bos taurus. [Genome view] ENSBTAT00000057547; ENSBTAP00000049725; ENSBTAG00000000445; Bos taurus. [Genome view]
GeneID	281975.
KEGG	bta:281975.
Organism-specific databases
CTD	281975.
Phylogenomic databases
HOVERGEN	P16586.
Enzyme and pathway databases
BRENDA	3.1.4.35. 251.
Family and domain databases
InterPro	IPR003018. GAF. IPR003607. Met-dep_phosphohydro_HD. IPR002073. PDEase. [Graphical view]
Pfam	PF01590. GAF. 2 hits. PF00233. PDEase_I. 1 hit. [Graphical view]
PRINTS	PR00387. PDIESTERASE1.
SMART	SM00065. GAF. 2 hits. SM00471. HDc. 1 hit. [Graphical view]
PROSITE	PS00126. PDEASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PDE6C_BOVIN

Accession

Primary (citable) accession number: P16586

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	October 13, 2009
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)