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P16586

- PDE6C_BOVIN

UniProt

P16586 - PDE6C_BOVIN

Protein

Cone cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha'

Gene

PDE6C

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 1 (01 Aug 1990)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.

    Cofactori

    Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei92 – 921cGMPBy similarity
    Binding sitei116 – 1161cGMPBy similarity
    Binding sitei171 – 1711cGMPBy similarity
    Active sitei557 – 5571Proton donorBy similarity
    Metal bindingi561 – 5611Divalent metal cation 1By similarity
    Metal bindingi597 – 5971Divalent metal cation 1By similarity
    Metal bindingi598 – 5981Divalent metal cation 1By similarity
    Metal bindingi598 – 5981Divalent metal cation 2By similarity
    Metal bindingi718 – 7181Divalent metal cation 1By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi164 – 1674cGMPBy similarity

    GO - Molecular functioni

    1. 3',5'-cyclic-GMP phosphodiesterase activity Source: UniProtKB-EC
    2. cGMP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. signal transduction Source: InterPro
    2. visual perception Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Sensory transduction, Vision

    Keywords - Ligandi

    cGMP, cGMP-binding, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cone cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha' (EC:3.1.4.35)
    Alternative name(s):
    PDE V-C1
    cGMP phosphodiesterase 6C
    Gene namesi
    Name:PDE6C
    Synonyms:PDEA2
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Unplaced

    Subcellular locationi

    Cell membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 852852Cone cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha'PRO_0000198830Add
    BLAST
    Propeptidei853 – 8553Removed in mature formSequence AnalysisPRO_0000370787

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei852 – 8521Cysteine methyl esterSequence Analysis
    Lipidationi852 – 8521S-geranylgeranyl cysteineBy similarity

    Keywords - PTMi

    Lipoprotein, Methylation, Prenylation

    Proteomic databases

    PRIDEiP16586.

    Interactioni

    Subunit structurei

    Composed of two alpha' subunits that are associated with 3 smaller proteins of 11, 13, and 15 kDa.

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000049725.

    Structurei

    3D structure databases

    ProteinModelPortaliP16586.
    SMRiP16586. Positions 52-220.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini70 – 219150GAF 1Add
    BLAST
    Domaini251 – 428178GAF 2Add
    BLAST

    Sequence similaritiesi

    Contains 2 GAF domains.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG290744.
    HOGENOMiHOG000007069.
    HOVERGENiHBG053539.
    KOiK13757.

    Family and domain databases

    Gene3Di1.10.1300.10. 1 hit.
    3.30.450.40. 3 hits.
    InterProiIPR003018. GAF.
    IPR029016. GAF_dom_like.
    IPR003607. HD/PDEase_dom.
    IPR023088. PDEase.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    [Graphical view]
    PfamiPF01590. GAF. 2 hits.
    PF00233. PDEase_I. 1 hit.
    [Graphical view]
    PRINTSiPR00387. PDIESTERASE1.
    SMARTiSM00065. GAF. 2 hits.
    SM00471. HDc. 1 hit.
    [Graphical view]
    SUPFAMiSSF55781. SSF55781. 3 hits.
    PROSITEiPS00126. PDEASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P16586-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGEISQETVE KYLEANPQFA KEYFNRKLQV EVPSGGAQAP ASASFPGRTL    50
    AEEAALYLEL LEVLLEEAGS VELAAHRALQ RLAQLLQADR CSMFLCRARN 100
    GTPEVASKLL DVTPTSKFED NLVVPDREAV FPLDVGIVGW VAHTKKTFNV 150
    PDVKKNSHFS DFMDKQTGYV TRNLLATPIV MGKEVLAVFM AVNKVDASEF 200
    SKQDEEVFSK YLSFVSIILK LHHTNYLYNI ESRRSQILMW SANKVFEELT 250
    DVERQFHKAL YTVRTYLNCE RYSIGLLDMT KEKEFYDEWP VKLGEVEPYK 300
    GPKTPDGREV IFYKIIDYIL HGKEEIKVIP TPPMDHWTLI SGLPTYVAEN 350
    GFICNMLNAP ADEYFTFQKG PVDETGWVIK NVLSLPIVNK KEDIVGVATF 400
    YNRKDGKPFD EYDEHIAETL TQFLGWSLLN TDTYEKMNKL ENRKDIAQEM 450
    LMNHTKATPD EIKSILKFKE KLNIDVIEDC EEKQLVTILK EDLPDPRTAD 500
    LYEFRFRHLP ITEHELIKCG LRLFFEINVV EKFKVPVEVL TRWMYTVRKG 550
    YRAVTYHNWR HGFNVGQTMF TLLMTGRLKK YYTDLEAFAM LAAAFCHDID 600
    HRGTNNLYQM KSTSPLARLH GSSILERHHL EYSKTLLQDE SLNIFQNLNK 650
    RQYETVIHLF EVAIIATDLA LYFKKRTMFQ KIVDACEKME TEEEAIKYVT 700
    IDPTKKEIIM AMMMTACDLS AITKPWEVQS QVALLVANEF WEQGDLERTV 750
    LQQQPIPMMD RNKKDELPKL QVGFIDFVCT FVYKEFSRFH KEITPMLNGL 800
    QNNRVEWKSL ADEYDEKMKV IEEMKKQEEG NTTEKAVEDS GGGGDDKKSK 850
    TCLML 855
    Length:855
    Mass (Da):98,798
    Last modified:August 1, 1990 - v1
    Checksum:i1FCFFFD045686D65
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M37838 mRNA. Translation: AAA30687.1.
    M33140 mRNA. Translation: AAA30688.1.
    PIRiA34810.
    RefSeqiNP_776844.1. NM_174419.1.
    UniGeneiBt.444.

    Genome annotation databases

    GeneIDi281975.
    KEGGibta:281975.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M37838 mRNA. Translation: AAA30687.1 .
    M33140 mRNA. Translation: AAA30688.1 .
    PIRi A34810.
    RefSeqi NP_776844.1. NM_174419.1.
    UniGenei Bt.444.

    3D structure databases

    ProteinModelPortali P16586.
    SMRi P16586. Positions 52-220.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000049725.

    Chemistry

    BindingDBi P16586.
    ChEMBLi CHEMBL2096979.

    Proteomic databases

    PRIDEi P16586.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 281975.
    KEGGi bta:281975.

    Organism-specific databases

    CTDi 5146.

    Phylogenomic databases

    eggNOGi NOG290744.
    HOGENOMi HOG000007069.
    HOVERGENi HBG053539.
    KOi K13757.

    Miscellaneous databases

    NextBioi 20805846.

    Family and domain databases

    Gene3Di 1.10.1300.10. 1 hit.
    3.30.450.40. 3 hits.
    InterProi IPR003018. GAF.
    IPR029016. GAF_dom_like.
    IPR003607. HD/PDEase_dom.
    IPR023088. PDEase.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    [Graphical view ]
    Pfami PF01590. GAF. 2 hits.
    PF00233. PDEase_I. 1 hit.
    [Graphical view ]
    PRINTSi PR00387. PDIESTERASE1.
    SMARTi SM00065. GAF. 2 hits.
    SM00471. HDc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55781. SSF55781. 3 hits.
    PROSITEi PS00126. PDEASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Bovine cone photoreceptor cGMP phosphodiesterase structure deduced from a cDNA clone."
      Li T., Volpp K., Applebury M.L.
      Proc. Natl. Acad. Sci. U.S.A. 87:293-297(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Identification of a noncatalytic cGMP-binding domain conserved in both the cGMP-stimulated and photoreceptor cyclic nucleotide phosphodiesterases."
      Charbonneau H., Prusti R.K., Letrong H., Sonnenburg W.K., Mullaney P.J., Walsh K., Beavo J.A.
      Proc. Natl. Acad. Sci. U.S.A. 87:288-292(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 308-502, PARTIAL PROTEIN SEQUENCE.

    Entry informationi

    Entry nameiPDE6C_BOVIN
    AccessioniPrimary (citable) accession number: P16586
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: August 1, 1990
    Last modified: October 1, 2014
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3