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Protein

Acidic endochitinase WIN6

Gene

WIN6

Organism
Populus trichocarpa (Western balsam poplar) (Populus balsamifera subsp. trichocarpa)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Defense against chitin containing fungal pathogens.

Catalytic activityi

Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

GO - Molecular functioni

  1. chitinase activity Source: UniProtKB-EC
  2. chitin binding Source: UniProtKB-KW

GO - Biological processi

  1. cell wall macromolecule catabolic process Source: InterPro
  2. chitin catabolic process Source: UniProtKB-KW
  3. defense response Source: UniProtKB-KW
  4. polysaccharide catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Chitin degradation, Plant defense, Polysaccharide degradation

Keywords - Ligandi

Chitin-binding

Protein family/group databases

CAZyiCBM18. Carbohydrate-Binding Module Family 18.
GH19. Glycoside Hydrolase Family 19.

Names & Taxonomyi

Protein namesi
Recommended name:
Acidic endochitinase WIN6 (EC:3.2.1.14)
Gene namesi
Name:WIN6
OrganismiPopulus trichocarpa (Western balsam poplar) (Populus balsamifera subsp. trichocarpa)
Taxonomic identifieri3694 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsMalpighialesSalicaceaeSaliceaePopulus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222By similarityAdd
BLAST
Chaini23 – 340318Acidic endochitinase WIN6PRO_0000005316Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi25 ↔ 40PROSITE-ProRule annotation
Disulfide bondi34 ↔ 46PROSITE-ProRule annotation
Disulfide bondi39 ↔ 53PROSITE-ProRule annotation
Disulfide bondi57 ↔ 61PROSITE-ProRule annotation
Disulfide bondi110 ↔ 172PROSITE-ProRule annotation
Disulfide bondi183 ↔ 191PROSITE-ProRule annotation
Disulfide bondi290 ↔ 323PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP16579.

Expressioni

Inductioni

By wounding.

Interactioni

Protein-protein interaction databases

STRINGi3694.grail3.0001024001.

Structurei

3D structure databases

ProteinModelPortaliP16579.
SMRiP16579. Positions 22-62, 89-330.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini23 – 6341Chitin-binding type-1PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni64 – 8522SpacerAdd
BLAST
Regioni86 – 340255ChitinaseAdd
BLAST

Sequence similaritiesi

Contains 1 chitin-binding type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3979.
HOGENOMiHOG000231411.

Family and domain databases

Gene3Di3.30.60.10. 1 hit.
InterProiIPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
IPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PfamiPF00187. Chitin_bind_1. 1 hit.
PF00182. Glyco_hydro_19. 1 hit.
[Graphical view]
PIRSFiPIRSF001060. Endochitinase. 1 hit.
PRINTSiPR00451. CHITINBINDNG.
ProDomiPD000609. Chitin_bd_1. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00270. ChtBD1. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF57016. SSF57016. 1 hit.
PROSITEiPS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 1 hit.
PS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P16579-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVWALFAFF SLFLSLSVRG SAEQCGRQAG DALCPGGLCC SSYGWCGTTV
60 70 80 90 100
DYCGIGCQSQ CDGGGGGDGG DDGCDGGDDG GGDGDDGYLS DIIPKSKFDA
110 120 130 140 150
LLKFRNDARC PAAGFYTYNA FISAAKEFPD FGNTGDDLMR KREIAAFLGQ
160 170 180 190 200
TSHETTGGWP DAPCGPYAWG YCYLKEINCQ PYCDPSSNYQ CVAGKQYCGR
210 220 230 240 250
GPIQLSWNYN YGLCGDDLKL PLLQEPELVE TDPVISFKTA IWFWMKPQSP
260 270 280 290 300
KPSCHAVITG NWTPSAADLE AGRVPGYGVI TNIINGGIEC GQGGPNAANE
310 320 330 340
DRIGFYKKYC DSLGTTYGSN LDCYQQRPFG YGLSGLKDTM
Length:340
Mass (Da):36,410
Last modified:September 30, 1996 - v2
Checksum:iC4E96473BEAA55C5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01661 Genomic DNA. Translation: AAA57278.1.
U01660 mRNA. Translation: AAA57277.1.
M25336 mRNA. Translation: AAA96701.1.
PIRiB33985.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01661 Genomic DNA. Translation: AAA57278.1.
U01660 mRNA. Translation: AAA57277.1.
M25336 mRNA. Translation: AAA96701.1.
PIRiB33985.

3D structure databases

ProteinModelPortaliP16579.
SMRiP16579. Positions 22-62, 89-330.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3694.grail3.0001024001.

Protein family/group databases

CAZyiCBM18. Carbohydrate-Binding Module Family 18.
GH19. Glycoside Hydrolase Family 19.

Proteomic databases

PRIDEiP16579.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiCOG3979.
HOGENOMiHOG000231411.

Family and domain databases

Gene3Di3.30.60.10. 1 hit.
InterProiIPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
IPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PfamiPF00187. Chitin_bind_1. 1 hit.
PF00182. Glyco_hydro_19. 1 hit.
[Graphical view]
PIRSFiPIRSF001060. Endochitinase. 1 hit.
PRINTSiPR00451. CHITINBINDNG.
ProDomiPD000609. Chitin_bd_1. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00270. ChtBD1. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF57016. SSF57016. 1 hit.
PROSITEiPS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 1 hit.
PS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Wound-induced and developmental activation of a poplar tree chitinase gene promoter in transgenic tobacco."
    Clarke H.R., Davis J.M., Wilbert S.M., Bradshaw H.D. Jr., Gordon M.P.
    Plant Mol. Biol. 25:799-815(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: H11-11.
    Tissue: Leaf.
  2. "Systemic accumulation of specific mRNAs in response to wounding in poplar trees."
    Parsons T.J., Bradshaw H.D. Jr., Gordon M.P.
    Proc. Natl. Acad. Sci. U.S.A. 86:7895-7899(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 127-340.

Entry informationi

Entry nameiCHI6_POPTR
AccessioniPrimary (citable) accession number: P16579
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 31, 1990
Last sequence update: September 30, 1996
Last modified: September 30, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.