P16579 (CHI6_POPTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acidic endochitinase WIN6
EC=3.2.1.14

Gene names

Name:

WIN6

Organism

Populus trichocarpa (Western balsam poplar) (Populus balsamifera subsp. trichocarpa)

Taxonomic identifier

3694 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Malpighiales › Salicaceae › Saliceae › Populus

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Defense against chitin containing fungal pathogens.
Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Induction	By wounding.
Sequence similarities	Belongs to the glycosyl hydrolase 19 family. Chitinase class I subfamily. Contains 1 chitin-binding type-1 domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Plant defense Polysaccharide degradation
Domain	Signal
Ligand	Chitin-binding
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chitin binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	By similarity
	Chain	23 – 340	318	Acidic endochitinase WIN6		PRO_0000005316
Regions
	Domain	23 – 63	41	Chitin-binding type-1
	Region	64 – 85	22	Spacer
	Region	86 – 340	255	Chitinase
Amino acid modifications
	Disulfide bond	25 ↔ 40		By similarity
	Disulfide bond	34 ↔ 46		By similarity
	Disulfide bond	39 ↔ 53		By similarity
	Disulfide bond	57 ↔ 61		By similarity
	Disulfide bond	110 ↔ 172		By similarity
	Disulfide bond	183 ↔ 191		By similarity
	Disulfide bond	290 ↔ 323		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P16579 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: C4E96473BEAA55C5	FASTA	340	36,410
	10 20 30 40 50 60 MSVWALFAFF SLFLSLSVRG SAEQCGRQAG DALCPGGLCC SSYGWCGTTV DYCGIGCQSQ 70 80 90 100 110 120 CDGGGGGDGG DDGCDGGDDG GGDGDDGYLS DIIPKSKFDA LLKFRNDARC PAAGFYTYNA 130 140 150 160 170 180 FISAAKEFPD FGNTGDDLMR KREIAAFLGQ TSHETTGGWP DAPCGPYAWG YCYLKEINCQ 190 200 210 220 230 240 PYCDPSSNYQ CVAGKQYCGR GPIQLSWNYN YGLCGDDLKL PLLQEPELVE TDPVISFKTA 250 260 270 280 290 300 IWFWMKPQSP KPSCHAVITG NWTPSAADLE AGRVPGYGVI TNIINGGIEC GQGGPNAANE 310 320 330 340 DRIGFYKKYC DSLGTTYGSN LDCYQQRPFG YGLSGLKDTM

References

[1]	"Wound-induced and developmental activation of a poplar tree chitinase gene promoter in transgenic tobacco." Clarke H.R., Davis J.M., Wilbert S.M., Bradshaw H.D. Jr., Gordon M.P. Plant Mol. Biol. 25:799-815(1994) [PubMed: 8075397] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: H11-11. Tissue: Leaf.
[2]	"Systemic accumulation of specific mRNAs in response to wounding in poplar trees." Parsons T.J., Bradshaw H.D. Jr., Gordon M.P. Proc. Natl. Acad. Sci. U.S.A. 86:7895-7899(1989) [PubMed: 2813366] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 127-340.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U01661 Genomic DNA. Translation: AAA57278.1. U01660 mRNA. Translation: AAA57277.1. M25336 mRNA. Translation: AAA96701.1.
PIR	B33985.
3D structure databases
ProteinModelPortal	P16579.
SMR	P16579. Positions 22-62, 89-330.
ModBase	Search...
Protein family/group databases
CAZy	CBM18. Carbohydrate-Binding Module Family 18. GH19. Glycoside Hydrolase Family 19.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001002. Chitin-bd_1. IPR018371. Chitin-binding_1_CS. IPR016283. Glyco_hydro_19. IPR000726. Glyco_hydro_19_cat. IPR023346. Lysozyme-like_dom. [Graphical view]
Gene3D	G3DSA:3.30.60.10. Chitin_bd_1. 1 hit.
Pfam	PF00187. Chitin_bind_1. 1 hit. PF00182. Glyco_hydro_19. 1 hit. [Graphical view]
PIRSF	PIRSF001060. Endochitinase. 1 hit.
PRINTS	PR00451. CHITINBINDNG.
ProDom	PD000609. Chitin_bd_1. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00270. ChtBD1. 1 hit. [Graphical view]
SUPFAM	SSF57016. Chitin_bd_1. 1 hit. SSF53955. SSF53955. 1 hit.
PROSITE	PS00026. CHIT_BIND_I_1. 1 hit. PS50941. CHIT_BIND_I_2. 1 hit. PS00773. CHITINASE_19_1. 1 hit. PS00774. CHITINASE_19_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHI6_POPTR

Accession

Primary (citable) accession number: P16579

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	October 1, 1996
Last modified:	November 16, 2011
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program