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P16579 (CHI6_POPTR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Acidic endochitinase WIN6

EC=3.2.1.14
Gene names
Name:WIN6
OrganismPopulus trichocarpa (Western balsam poplar) (Populus balsamifera subsp. trichocarpa)
Taxonomic identifier3694 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsMalpighialesSalicaceaeSaliceaePopulus

Protein attributes

Sequence length340 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Defense against chitin containing fungal pathogens.

Catalytic activity

Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

Induction

By wounding.

Sequence similarities

Belongs to the glycosyl hydrolase 19 family. Chitinase class I subfamily.

Contains 1 chitin-binding type-1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 By similarity
Chain23 – 340318Acidic endochitinase WIN6
PRO_0000005316

Regions

Domain23 – 6341Chitin-binding type-1
Region64 – 8522Spacer
Region86 – 340255Chitinase

Amino acid modifications

Disulfide bond25 ↔ 40 By similarity
Disulfide bond34 ↔ 46 By similarity
Disulfide bond39 ↔ 53 By similarity
Disulfide bond57 ↔ 61 By similarity
Disulfide bond110 ↔ 172 By similarity
Disulfide bond183 ↔ 191 By similarity
Disulfide bond290 ↔ 323 By similarity

Sequences

Sequence LengthMass (Da)Tools
P16579 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: C4E96473BEAA55C5

FASTA34036,410
        10         20         30         40         50         60 
MSVWALFAFF SLFLSLSVRG SAEQCGRQAG DALCPGGLCC SSYGWCGTTV DYCGIGCQSQ 

        70         80         90        100        110        120 
CDGGGGGDGG DDGCDGGDDG GGDGDDGYLS DIIPKSKFDA LLKFRNDARC PAAGFYTYNA 

       130        140        150        160        170        180 
FISAAKEFPD FGNTGDDLMR KREIAAFLGQ TSHETTGGWP DAPCGPYAWG YCYLKEINCQ 

       190        200        210        220        230        240 
PYCDPSSNYQ CVAGKQYCGR GPIQLSWNYN YGLCGDDLKL PLLQEPELVE TDPVISFKTA 

       250        260        270        280        290        300 
IWFWMKPQSP KPSCHAVITG NWTPSAADLE AGRVPGYGVI TNIINGGIEC GQGGPNAANE 

       310        320        330        340 
DRIGFYKKYC DSLGTTYGSN LDCYQQRPFG YGLSGLKDTM 

« Hide

References

[1]"Wound-induced and developmental activation of a poplar tree chitinase gene promoter in transgenic tobacco."
Clarke H.R., Davis J.M., Wilbert S.M., Bradshaw H.D. Jr., Gordon M.P.
Plant Mol. Biol. 25:799-815(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Strain: H11-11.
Tissue: Leaf.
[2]"Systemic accumulation of specific mRNAs in response to wounding in poplar trees."
Parsons T.J., Bradshaw H.D. Jr., Gordon M.P.
Proc. Natl. Acad. Sci. U.S.A. 86:7895-7899(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 127-340.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U01661 Genomic DNA. Translation: AAA57278.1.
U01660 mRNA. Translation: AAA57277.1.
M25336 mRNA. Translation: AAA96701.1.
PIRB33985.

3D structure databases

ProteinModelPortalP16579.
SMRP16579. Positions 22-62, 89-330.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3694.grail3.0001024001.

Protein family/group databases

CAZyCBM18. Carbohydrate-Binding Module Family 18.
GH19. Glycoside Hydrolase Family 19.

Proteomic databases

PRIDEP16579.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGCOG3979.
HOGENOMHOG000231411.

Family and domain databases

Gene3D3.30.60.10. 1 hit.
InterProIPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
IPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PfamPF00187. Chitin_bind_1. 1 hit.
PF00182. Glyco_hydro_19. 1 hit.
[Graphical view]
PIRSFPIRSF001060. Endochitinase. 1 hit.
PRINTSPR00451. CHITINBINDNG.
ProDomPD000609. Chitin_bd_1. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00270. ChtBD1. 1 hit.
[Graphical view]
SUPFAMSSF53955. SSF53955. 1 hit.
SSF57016. SSF57016. 1 hit.
PROSITEPS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 1 hit.
PS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCHI6_POPTR
AccessionPrimary (citable) accession number: P16579
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries