ID UBC4_WHEAT Reviewed; 184 AA. AC P16577; DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot. DT 01-AUG-1990, sequence version 1. DT 27-MAR-2024, entry version 126. DE RecName: Full=Ubiquitin-conjugating enzyme E2-23 kDa; DE EC=2.3.2.23; DE AltName: Full=E2 ubiquitin-conjugating enzyme; DE AltName: Full=Ubiquitin carrier protein; DE AltName: Full=Ubiquitin-protein ligase; GN Name=UBC4; OS Triticum aestivum (Wheat). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade; OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum. OX NCBI_TaxID=4565; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RC STRAIN=cv. Augusta; RX PubMed=2557633; DOI=10.1073/pnas.86.24.9861; RA Sullivan M.L., Vierstra R.D.; RT "A ubiquitin carrier protein from wheat germ is structurally and RT functionally similar to the yeast DNA repair enzyme encoded by RAD6."; RL Proc. Natl. Acad. Sci. U.S.A. 86:9861-9865(1989). RN [2] RP MUTAGENESIS OF CYS-85. RX PubMed=1660887; DOI=10.1016/s0021-9258(18)54365-9; RA Sullivan M.L., Vierstra R.D.; RT "Cloning of a 16-kDa ubiquitin carrier protein from wheat and Arabidopsis RT thaliana. Identification of functional domains by in vitro mutagenesis."; RL J. Biol. Chem. 266:23878-23885(1991). CC -!- FUNCTION: Catalyzes the covalent attachment of ubiquitin to other CC proteins. CC -!- CATALYTIC ACTIVITY: CC Reaction=S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + CC [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin- CC activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin- CC conjugating enzyme]-L-cysteine.; EC=2.3.2.23; CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00388, ECO:0000255|PROSITE- CC ProRule:PRU10133}; CC -!- PATHWAY: Protein modification; protein ubiquitination. CC {ECO:0000255|PROSITE-ProRule:PRU00388}. CC -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family. CC {ECO:0000255|PROSITE-ProRule:PRU00388}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; M28059; AAA34309.1; -; mRNA. DR PIR; A34506; A34506. DR AlphaFoldDB; P16577; -. DR SMR; P16577; -. DR STRING; 4565.P16577; -. DR PaxDb; 4565-Traes_5DL_51681402F-1; -. DR EnsemblPlants; TraesCAD_scaffold_095608_01G000100.1; TraesCAD_scaffold_095608_01G000100.1; TraesCAD_scaffold_095608_01G000100. DR EnsemblPlants; TraesCLE_scaffold_012902_01G000500.1; TraesCLE_scaffold_012902_01G000500.1; TraesCLE_scaffold_012902_01G000500. DR EnsemblPlants; TraesCS4A02G313600.1; TraesCS4A02G313600.1; TraesCS4A02G313600. DR EnsemblPlants; TraesCS4A03G0783100.1; TraesCS4A03G0783100.1.CDS; TraesCS4A03G0783100. DR EnsemblPlants; TraesKAR4A01G0371660.1; cds.TraesKAR4A01G0371660.1; TraesKAR4A01G0371660. DR EnsemblPlants; TraesPAR_scaffold_128789_01G000300.1; TraesPAR_scaffold_128789_01G000300.1; TraesPAR_scaffold_128789_01G000300. DR EnsemblPlants; TraesROB_scaffold_016095_01G000100.1; TraesROB_scaffold_016095_01G000100.1; TraesROB_scaffold_016095_01G000100. DR EnsemblPlants; TraesWEE_scaffold_010029_01G000500.1; TraesWEE_scaffold_010029_01G000500.1; TraesWEE_scaffold_010029_01G000500. DR Gramene; TraesCAD_scaffold_095608_01G000100.1; TraesCAD_scaffold_095608_01G000100.1; TraesCAD_scaffold_095608_01G000100. DR Gramene; TraesCLE_scaffold_012902_01G000500.1; TraesCLE_scaffold_012902_01G000500.1; TraesCLE_scaffold_012902_01G000500. DR Gramene; TraesCS4A02G313600.1; TraesCS4A02G313600.1; TraesCS4A02G313600. DR Gramene; TraesCS4A03G0783100.1; TraesCS4A03G0783100.1.CDS; TraesCS4A03G0783100. DR Gramene; TraesKAR4A01G0371660.1; cds.TraesKAR4A01G0371660.1; TraesKAR4A01G0371660. DR Gramene; TraesPAR_scaffold_128789_01G000300.1; TraesPAR_scaffold_128789_01G000300.1; TraesPAR_scaffold_128789_01G000300. DR Gramene; TraesROB_scaffold_016095_01G000100.1; TraesROB_scaffold_016095_01G000100.1; TraesROB_scaffold_016095_01G000100. DR Gramene; TraesWEE_scaffold_010029_01G000500.1; TraesWEE_scaffold_010029_01G000500.1; TraesWEE_scaffold_010029_01G000500. DR eggNOG; KOG0416; Eukaryota. DR OMA; LMMSDWK; -. DR OrthoDB; 619890at2759; -. DR UniPathway; UPA00143; -. DR Proteomes; UP000019116; Chromosome 4A. DR GO; GO:0005634; C:nucleus; IBA:GO_Central. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0061631; F:ubiquitin conjugating enzyme activity; IBA:GO_Central. DR GO; GO:0000209; P:protein polyubiquitination; IBA:GO_Central. DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IBA:GO_Central. DR CDD; cd00195; UBCc; 1. DR Gene3D; 3.10.110.10; Ubiquitin Conjugating Enzyme; 1. DR InterPro; IPR000608; UBQ-conjugat_E2. DR InterPro; IPR023313; UBQ-conjugating_AS. DR InterPro; IPR016135; UBQ-conjugating_enzyme/RWD. DR PANTHER; PTHR24068; UBIQUITIN-CONJUGATING ENZYME E2; 1. DR PANTHER; PTHR24068:SF518; UBIQUITIN-CONJUGATING ENZYME E2-23 KDA; 1. DR Pfam; PF00179; UQ_con; 1. DR SMART; SM00212; UBCc; 1. DR SUPFAM; SSF54495; UBC-like; 1. DR PROSITE; PS00183; UBC_1; 1. DR PROSITE; PS50127; UBC_2; 1. PE 1: Evidence at protein level; KW ATP-binding; Nucleotide-binding; Reference proteome; Transferase; KW Ubl conjugation pathway. FT CHAIN 1..184 FT /note="Ubiquitin-conjugating enzyme E2-23 kDa" FT /id="PRO_0000082581" FT DOMAIN 1..148 FT /note="UBC core" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00388" FT REGION 146..184 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 153..171 FT /note="Acidic residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT ACT_SITE 85 FT /note="Glycyl thioester intermediate" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00388" FT MUTAGEN 85 FT /note="C->S: Loss of activity." FT /evidence="ECO:0000269|PubMed:1660887" SQ SEQUENCE 184 AA; 21126 MW; AA479C4E42C893B7 CRC64; MSSPSKRREM DLMKLMMSDY KVDMINDGMH EFFVHFHGPK DSIYQGGVWK VRVELTEAYP YKSPSIGFTN KIYHPNVDEM SGSVCLDVIN QTWSPMFDLV NIFEVFLPQL LLYPNPSDPL NGEAASLMMR DKNAYENKVK EYCERYAKPE DISPEEEEEE SDEELSDAEG YDSGDEAIMG HADP //