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Protein

Ubiquitin-conjugating enzyme E2-23 kDa

Gene

UBC4

Organism
Triticum aestivum (Wheat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins.

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei85Glycyl thioester intermediate1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2-23 kDa (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme
Ubiquitin carrier protein
Ubiquitin-protein ligase
Gene namesi
Name:UBC4
OrganismiTriticum aestivum (Wheat)
Taxonomic identifieri4565 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladePooideaeTriticodaeTriticeaeTriticinaeTriticum
Proteomesi
  • UP000019116 Componenti: Unplaced

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi85C → S: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000825811 – 184Ubiquitin-conjugating enzyme E2-23 kDaAdd BLAST184

Structurei

3D structure databases

ProteinModelPortaliP16577.
SMRiP16577.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi141 – 176Asp/Glu-rich (acidic)Add BLAST36

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Family and domain databases

CDDicd00195. UBCc. 1 hit.
Gene3Di3.10.110.10. 1 hit.
InterProiView protein in InterPro
IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
PfamiView protein in Pfam
PF00179. UQ_con. 1 hit.
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiView protein in PROSITE
PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P16577-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSPSKRREM DLMKLMMSDY KVDMINDGMH EFFVHFHGPK DSIYQGGVWK
60 70 80 90 100
VRVELTEAYP YKSPSIGFTN KIYHPNVDEM SGSVCLDVIN QTWSPMFDLV
110 120 130 140 150
NIFEVFLPQL LLYPNPSDPL NGEAASLMMR DKNAYENKVK EYCERYAKPE
160 170 180
DISPEEEEEE SDEELSDAEG YDSGDEAIMG HADP
Length:184
Mass (Da):21,126
Last modified:August 1, 1990 - v1
Checksum:iAA479C4E42C893B7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28059 mRNA. Translation: AAA34309.1.
PIRiA34506.
UniGeneiTa.96.

Genome annotation databases

EnsemblPlantsiTRIAE_CS42_4AL_TGACv1_290774_AA0989180.1; TRIAE_CS42_4AL_TGACv1_290774_AA0989180.1; TRIAE_CS42_4AL_TGACv1_290774_AA0989180.
GrameneiTRIAE_CS42_4AL_TGACv1_290774_AA0989180.1; TRIAE_CS42_4AL_TGACv1_290774_AA0989180.1; TRIAE_CS42_4AL_TGACv1_290774_AA0989180.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28059 mRNA. Translation: AAA34309.1.
PIRiA34506.
UniGeneiTa.96.

3D structure databases

ProteinModelPortaliP16577.
SMRiP16577.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiTRIAE_CS42_4AL_TGACv1_290774_AA0989180.1; TRIAE_CS42_4AL_TGACv1_290774_AA0989180.1; TRIAE_CS42_4AL_TGACv1_290774_AA0989180.
GrameneiTRIAE_CS42_4AL_TGACv1_290774_AA0989180.1; TRIAE_CS42_4AL_TGACv1_290774_AA0989180.1; TRIAE_CS42_4AL_TGACv1_290774_AA0989180.

Enzyme and pathway databases

UniPathwayiUPA00143.

Family and domain databases

CDDicd00195. UBCc. 1 hit.
Gene3Di3.10.110.10. 1 hit.
InterProiView protein in InterPro
IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
PfamiView protein in Pfam
PF00179. UQ_con. 1 hit.
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiView protein in PROSITE
PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiUBC4_WHEAT
AccessioniPrimary (citable) accession number: P16577
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: May 10, 2017
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.