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P16577 (UBC4_WHEAT) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin-conjugating enzyme E2-23 kDa

EC=6.3.2.19
Alternative name(s):
Ubiquitin carrier protein
Ubiquitin-protein ligase
Gene names
Name:UBC4
OrganismTriticum aestivum (Wheat)
Taxonomic identifier4565 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeTriticum

Protein attributes

Sequence length184 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the covalent attachment of ubiquitin to other proteins.

Catalytic activity

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathway

Protein modification; protein ubiquitination.

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
Gene Ontology (GO)
   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ubiquitin-protein ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 184184Ubiquitin-conjugating enzyme E2-23 kDa
PRO_0000082581

Regions

Compositional bias141 – 17636Asp/Glu-rich (acidic)

Sites

Active site851Glycyl thioester intermediate

Experimental info

Mutagenesis851C → S: Loss of activity. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P16577 [UniParc].

Last modified August 1, 1990. Version 1.
Checksum: AA479C4E42C893B7

FASTA18421,126
        10         20         30         40         50         60 
MSSPSKRREM DLMKLMMSDY KVDMINDGMH EFFVHFHGPK DSIYQGGVWK VRVELTEAYP 

        70         80         90        100        110        120 
YKSPSIGFTN KIYHPNVDEM SGSVCLDVIN QTWSPMFDLV NIFEVFLPQL LLYPNPSDPL 

       130        140        150        160        170        180 
NGEAASLMMR DKNAYENKVK EYCERYAKPE DISPEEEEEE SDEELSDAEG YDSGDEAIMG 


HADP 

« Hide

References

[1]"A ubiquitin carrier protein from wheat germ is structurally and functionally similar to the yeast DNA repair enzyme encoded by RAD6."
Sullivan M.L., Vierstra R.D.
Proc. Natl. Acad. Sci. U.S.A. 86:9861-9865(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Augusta.
[2]"Cloning of a 16-kDa ubiquitin carrier protein from wheat and Arabidopsis thaliana. Identification of functional domains by in vitro mutagenesis."
Sullivan M.L., Vierstra R.D.
J. Biol. Chem. 266:23878-23885(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF CYS-85.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M28059 mRNA. Translation: AAA34309.1.
PIRA34506.
UniGeneTa.96.

3D structure databases

ProteinModelPortalP16577.
SMRP16577. Positions 7-150.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

GrameneP16577.

Enzyme and pathway databases

UniPathwayUPA00143.

Family and domain databases

Gene3D3.10.110.10. 1 hit.
InterProIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMSSF54495. SSF54495. 1 hit.
PROSITEPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUBC4_WHEAT
AccessionPrimary (citable) accession number: P16577
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: October 16, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways