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Protein

Virulence sensor protein BvgS

Gene

bvgS

Organism
Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system BvgS/BvgA. Phosphorylates BvgA via a four-step phosphorelay in response to environmental signals.

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processTwo-component regulatory system, Virulence
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.13.3. 899.

Names & Taxonomyi

Protein namesi
Recommended name:
Virulence sensor protein BvgS (EC:2.7.13.3)
Gene namesi
Name:bvgS
Ordered Locus Names:BP1877
OrganismiBordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251)
Taxonomic identifieri257313 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesAlcaligenaceaeBordetella
Proteomesi
  • UP000002676 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini33 – 307CytoplasmicSequence analysisAdd BLAST275
Transmembranei308 – 331HelicalSequence analysisAdd BLAST24
Topological domaini332 – 541PeriplasmicSequence analysisAdd BLAST210
Transmembranei542 – 563HelicalSequence analysisAdd BLAST22
Topological domaini564 – 1238CytoplasmicSequence analysisAdd BLAST675

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi729H → Q: Loss of autophosphorylation. 1 Publication1
Mutagenesisi979D → G: Loss of activity; when associated with G-980. 1 Publication1
Mutagenesisi980D → G: Loss of activity. 1 Publication1
Mutagenesisi1023D → G or N: Loss of activity. 2 Publications1
Mutagenesisi1080K → L: Loss of activity. 1 Publication1
Mutagenesisi1146 – 1147Missing : Loss of activity. 2
Mutagenesisi1172H → Q: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 32Sequence analysisAdd BLAST32
ChainiPRO_000003237033 – 1238Virulence sensor protein BvgSAdd BLAST1206

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei729Phosphohistidine; by autocatalysisCurated1
Modified residuei10234-aspartylphosphateCurated1
Modified residuei1172PhosphohistidineCurated1

Post-translational modificationi

Activation requires a sequential transfer of a phosphate group from a His in the primary transmitter domain, to an Asp in the receiver domain and to a His in the secondary transmitter domain.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP16575.

Interactioni

Protein-protein interaction databases

STRINGi257313.BP1877.

Structurei

Secondary structure

11238
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi34 – 38Combined sources5
Helixi54 – 62Combined sources9
Beta strandi64 – 70Combined sources7
Turni75 – 77Combined sources3
Beta strandi79 – 81Combined sources3
Beta strandi84 – 87Combined sources4
Helixi88 – 99Combined sources12
Beta strandi103 – 111Combined sources9
Helixi112 – 120Combined sources9
Beta strandi125 – 130Combined sources6
Beta strandi134 – 137Combined sources4
Beta strandi139 – 142Combined sources4
Beta strandi151 – 156Combined sources6
Helixi164 – 167Combined sources4
Beta strandi170 – 175Combined sources6
Turni176 – 178Combined sources3
Helixi181 – 187Combined sources7
Beta strandi191 – 198Combined sources8
Helixi199 – 207Combined sources9
Beta strandi212 – 217Combined sources6
Helixi218 – 228Combined sources11
Turni229 – 232Combined sources4
Beta strandi233 – 238Combined sources6
Beta strandi246 – 251Combined sources6
Helixi255 – 267Combined sources13
Helixi270 – 279Combined sources10
Helixi283 – 285Combined sources3
Turni286 – 288Combined sources3
Helixi297 – 305Combined sources9
Beta strandi307 – 314Combined sources8
Turni318 – 320Combined sources3
Beta strandi321 – 323Combined sources3
Beta strandi329 – 331Combined sources3
Helixi332 – 344Combined sources13
Beta strandi347 – 355Combined sources9
Helixi356 – 365Combined sources10
Beta strandi369 – 375Combined sources7
Helixi378 – 380Combined sources3
Turni381 – 383Combined sources3
Beta strandi384 – 386Combined sources3
Beta strandi390 – 393Combined sources4
Beta strandi395 – 402Combined sources8
Helixi409 – 411Combined sources3
Beta strandi416 – 420Combined sources5
Helixi425 – 432Combined sources8
Beta strandi436 – 443Combined sources8
Helixi444 – 452Combined sources9
Beta strandi457 – 462Combined sources6
Helixi463 – 473Combined sources11
Turni475 – 477Combined sources3
Beta strandi478 – 483Combined sources6
Beta strandi489 – 496Combined sources8
Helixi500 – 511Combined sources12
Helixi515 – 523Combined sources9
Helixi532 – 544Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3MPKX-ray2.04A287-542[»]
3MPLX-ray2.10A287-542[»]
4Q0CX-ray3.10A/B/C/D30-544[»]
ProteinModelPortaliP16575.
SMRiP16575.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP16575.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini580 – 651PASPROSITE-ProRule annotationAdd BLAST72
Domaini652 – 708PACPROSITE-ProRule annotationAdd BLAST57
Domaini726 – 948Histidine kinasePROSITE-ProRule annotationAdd BLAST223
Domaini974 – 1095Response regulatoryPROSITE-ProRule annotationAdd BLAST122
Domaini1133 – 1228HPtPROSITE-ProRule annotationAdd BLAST96

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.
HOGENOMiHOG000269369.
KOiK07679.
OMAiHWILPYR.

Family and domain databases

CDDicd00130. PAS. 1 hit.
Gene3Di3.30.565.10. 1 hit.
InterProiView protein in InterPro
IPR011006. CheY-like_superfamily.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR013767. PAS_fold.
IPR004358. Sig_transdc_His_kin-like_C.
IPR008207. Sig_transdc_His_kin_Hpt_dom.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR001638. Solute-binding_3/MltF_N.
PfamiView protein in Pfam
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF01627. Hpt. 1 hit.
PF00989. PAS. 1 hit.
PF00072. Response_reg. 1 hit.
PF00497. SBP_bac_3. 2 hits.
PRINTSiPR00344. BCTRLSENSOR.
SMARTiView protein in SMART
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00073. HPT. 1 hit.
SM00091. PAS. 1 hit.
SM00062. PBPb. 2 hits.
SM00448. REC. 1 hit.
SUPFAMiSSF47226. SSF47226. 2 hits.
SSF47384. SSF47384. 1 hit.
SSF52172. SSF52172. 1 hit.
SSF55785. SSF55785. 1 hit.
SSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR00229. sensory_box. 1 hit.
PROSITEiView protein in PROSITE
PS50109. HIS_KIN. 1 hit.
PS50894. HPT. 1 hit.
PS50113. PAC. 1 hit.
PS50112. PAS. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P16575-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPAPHRLYPR SLICLAQALL AWALLAWAPA QASQELTLVG KAAVPDVEVA
60 70 80 90 100
LDGDDWRWLA RKRVLTLGVY APDIPPFDVT YGERYEGLTA DYMAIIAHNL
110 120 130 140 150
GMQAKVLRYP TREQALSALE SGQIDLIGTV NGTDGRQQSL RLSVPYAADH
160 170 180 190 200
PVIVMPIGAR HVPASNLAGQ RLAVDINYLP KETLARAYPQ ATLHYFPSSE
210 220 230 240 250
QALAAVAYGQ ADVFIGDALT TSHLVSQSYF NDVRVVAPAH IATGGESFGV
260 270 280 290 300
RADNTRLLRV VNAVLEAIPP SEHRSLIYRW GLGSSISLDF AHPAYSAREQ
310 320 330 340 350
QWMADHPVVK VAVLNLFAPF TLFRTDEQFG GISAAVLQLL QLRTGLDFEI
360 370 380 390 400
IGVDTVEELI AKLRSGEADM AGALFVNSAR ESFLSFSRPY VRNGMVIVTR
410 420 430 440 450
QDPDAPVDAD HLDGRTVALV RNSAAIPLLQ RRYPQAKVVT ADNPSEAMLM
460 470 480 490 500
VANGQADAVV QTQISASYYV NRYFAGKLRI ASALDLPPAE IALATTRGQT
510 520 530 540 550
ELMSILNKAL YSISNDELAS IISRWRGSDG DPRTWYAYRN EIYLLIGLGL
560 570 580 590 600
LSALLFLSWI VYLRRQIRQR KRAERALNDQ LEFMRVLIDG TPNPIYVRDK
610 620 630 640 650
EGRMLLCNDA YLDTFGVTAD AVLGKTIPEA NVVGDPALAR EMHEFLLTRV
660 670 680 690 700
AAEREPRFED RDVTLHGRTR HVYQWTIPYG DSLGELKGII GGWIDITERA
710 720 730 740 750
ELLRKLHDAK ESADAANRAK TTFLATMSHE IRTPMNAIIG MLELALLRPT
760 770 780 790 800
DQEPDRQSIQ VAYDSARSLL ELIGDILDIA KIEAGKFDLA PVRTALRVLP
810 820 830 840 850
EGAIRVFDGL ARQKGIELVL KTDIVGVDDV LIDPLRMKQV LSNLVGNAIK
860 870 880 890 900
FTTEGQVVLA VTARPDGDAA HVQFSVSDTG CGISEADQRQ LFKPFSQVGG
910 920 930 940 950
SAEAGPAPGT GLGLSISRRL VELMGGTLVM RSAPGVGTTV SVDLRLTMVE
960 970 980 990 1000
KSVQAAPPAA ATAATPSKPQ VSLRVLVVDD HKPNLMLLRQ QLDYLGQRVI
1010 1020 1030 1040 1050
AADSGEAALA LWREHAFDVV ITDCNMPGIS GYELARRIRA AEAAPGYGRT
1060 1070 1080 1090 1100
RCILFGFTAS AQMDEAQRCR AAGMDDCLFK PIGVDALRQR LNEAVARAAL
1110 1120 1130 1140 1150
PTPPSPQAAA PATDDATPTA FSAESILALT QNDEALIRQL LEEVIRTNRA
1160 1170 1180 1190 1200
DVDQLQKLHQ QADWPKVSDM AHRLAGGARV VDAKAMIDTV LALEKKAQGQ
1210 1220 1230
AGPSPEIDGL VRTLAAQSAA LETQLRAWLE QRPHQDQP
Length:1,238
Mass (Da):135,001
Last modified:September 19, 2003 - v3
Checksum:i28433439765ABC66
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti705K → E (PubMed:2549542).Curated1
Sequence conflicti705K → E (PubMed:1791760).Curated1
Sequence conflicti1068R → A (PubMed:2549542).Curated1
Sequence conflicti1068R → A (PubMed:1791760).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25401 Genomic DNA. Translation: AAA22970.1.
BX640416 Genomic DNA. Translation: CAE42160.1.
PIRiA40185.
RefSeqiNP_880569.1. NC_002929.2.
WP_010930608.1. NC_002929.2.

Genome annotation databases

EnsemblBacteriaiCAE42160; CAE42160; BP1877.
GeneIDi2667057.
KEGGibpe:BP1877.
PATRICifig|257313.5.peg.2016.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25401 Genomic DNA. Translation: AAA22970.1.
BX640416 Genomic DNA. Translation: CAE42160.1.
PIRiA40185.
RefSeqiNP_880569.1. NC_002929.2.
WP_010930608.1. NC_002929.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3MPKX-ray2.04A287-542[»]
3MPLX-ray2.10A287-542[»]
4Q0CX-ray3.10A/B/C/D30-544[»]
ProteinModelPortaliP16575.
SMRiP16575.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi257313.BP1877.

Proteomic databases

PRIDEiP16575.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAE42160; CAE42160; BP1877.
GeneIDi2667057.
KEGGibpe:BP1877.
PATRICifig|257313.5.peg.2016.

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.
HOGENOMiHOG000269369.
KOiK07679.
OMAiHWILPYR.

Enzyme and pathway databases

BRENDAi2.7.13.3. 899.

Miscellaneous databases

EvolutionaryTraceiP16575.

Family and domain databases

CDDicd00130. PAS. 1 hit.
Gene3Di3.30.565.10. 1 hit.
InterProiView protein in InterPro
IPR011006. CheY-like_superfamily.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR013767. PAS_fold.
IPR004358. Sig_transdc_His_kin-like_C.
IPR008207. Sig_transdc_His_kin_Hpt_dom.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR001638. Solute-binding_3/MltF_N.
PfamiView protein in Pfam
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF01627. Hpt. 1 hit.
PF00989. PAS. 1 hit.
PF00072. Response_reg. 1 hit.
PF00497. SBP_bac_3. 2 hits.
PRINTSiPR00344. BCTRLSENSOR.
SMARTiView protein in SMART
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00073. HPT. 1 hit.
SM00091. PAS. 1 hit.
SM00062. PBPb. 2 hits.
SM00448. REC. 1 hit.
SUPFAMiSSF47226. SSF47226. 2 hits.
SSF47384. SSF47384. 1 hit.
SSF52172. SSF52172. 1 hit.
SSF55785. SSF55785. 1 hit.
SSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR00229. sensory_box. 1 hit.
PROSITEiView protein in PROSITE
PS50109. HIS_KIN. 1 hit.
PS50894. HPT. 1 hit.
PS50113. PAC. 1 hit.
PS50112. PAS. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBVGS_BORPE
AccessioniPrimary (citable) accession number: P16575
Secondary accession number(s): P16576
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: September 19, 2003
Last modified: June 7, 2017
This is version 158 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be two separate ORFs named bvgB and bvgC.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.