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P16573 (CEAM1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Carcinoembryonic antigen-related cell adhesion molecule 1
Alternative name(s):
ATP-dependent taurocolate-carrier protein
Cell-CAM 105
Short name=C-CAM 105
Ecto-ATPase
GP110
pp120
CD_antigen=CD66a
Gene names
Name:Ceacam1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length519 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This protein is a calcium-independent cell adhesion molecule with homophilic binding properties. May play a role in the formation and maintenance of the specialized membrane structure of the apical surface of the hepatocytes.

Subcellular location

Cell membrane; Single-pass type I membrane protein. Note: Canalicular domain of hepatocyte plasma membranes.

Tissue specificity

Expressed in epithelia, vessel endothelia, leukocytes and platelets.

Post-translational modification

Phosphorylated on serine residues in both isoforms. On serine and tyrosine residues in cytoplasmic region of the long isoform. Ref.8

Sequence similarities

Belongs to the immunoglobulin superfamily. CEA family.

Contains 3 Ig-like C2-type (immunoglobulin-like) domains.

Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Long (identifier: P16573-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: P16573-2)

Also known as: C-CAM3;

The sequence of this isoform differs from the canonical sequence as follows:
     455-458: GSDH → SGSF
     459-519: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3434 By similarity
Chain35 – 519485Carcinoembryonic antigen-related cell adhesion molecule 1
PRO_0000014564

Regions

Topological domain35 – 422388Extracellular Potential
Transmembrane423 – 44826Helical; Potential
Topological domain449 – 51971Cytoplasmic Potential
Domain42 – 14099Ig-like V-type
Domain147 – 23286Ig-like C2-type 1
Domain237 – 31781Ig-like C2-type 2
Domain325 – 40379Ig-like C2-type 3

Amino acid modifications

Modified residue351Pyrrolidone carboxylic acid By similarity
Glycosylation871N-linked (GlcNAc...)
Glycosylation1041N-linked (GlcNAc...) Potential
Glycosylation1131N-linked (GlcNAc...) Potential
Glycosylation1481N-linked (GlcNAc...) Potential
Glycosylation1521N-linked (GlcNAc...) Potential
Glycosylation1721N-linked (GlcNAc...) Potential
Glycosylation1731N-linked (GlcNAc...) Potential
Glycosylation1971N-linked (GlcNAc...) Potential
Glycosylation2241N-linked (GlcNAc...) Potential
Glycosylation2561N-linked (GlcNAc...) Potential
Glycosylation2881N-linked (GlcNAc...) Potential
Glycosylation2921N-linked (GlcNAc...) Potential
Glycosylation3021N-linked (GlcNAc...) Potential
Glycosylation3151N-linked (GlcNAc...) Potential
Glycosylation3311N-linked (GlcNAc...) Potential
Glycosylation3741N-linked (GlcNAc...) Potential
Disulfide bond167 ↔ 215 By similarity
Disulfide bond259 ↔ 299 By similarity
Disulfide bond344 ↔ 392 By similarity

Natural variations

Alternative sequence455 – 4584GSDH → SGSF in isoform Short.
VSP_002504
Alternative sequence459 – 51961Missing in isoform Short.
VSP_002505
Natural variant491S → K.
Natural variant551T → A.
Natural variant701V → G.
Natural variant73 – 764TGLN → LNPD.
Natural variant861S → D.
Natural variant881T → M.
Natural variant901Q → K.
Natural variant921E → G.
Natural variant991V → E.
Natural variant118 – 1192GP → RA.
Natural variant1251I → F.
Natural variant1271K → Q.

Sequences

Sequence LengthMass (Da)Tools
Isoform Long [UniParc].

Last modified October 1, 1994. Version 3.
Checksum: 04143928D7975776

FASTA51957,264
        10         20         30         40         50         60 
MELASARLLR GQIPWRGLLL TASLLTYWSP LTTAQVTVDA VPPNVVEESS VLLLTHNLPQ 

        70         80         90        100        110        120 
EFQVFYWYKV TTTGLNSEIA RYIRSSNTSQ TEPAYSGRVT IYSNGSLFFQ NVNKTDEGPY 

       130        140        150        160        170        180 
TLSVIDKQFN PIQTSVQFRV YPALQKPNVT GNNSNPMEGE PFVSLMCEPY TNNTSYLWSR 

       190        200        210        220        230        240 
NGESLSEGDR VTFSEGNRTL TLLNVRRTDK GYYECEARNP ATFNRSDPFN LDVIYGPDAP 

       250        260        270        280        290        300 
VISPPDIYLH QGSNLNLSCH ADSNPPAQYF WLINEKLQTS SQELFISNIT TNNSGTYACF 

       310        320        330        340        350        360 
VNNTVTGLSR TTVKNITVFE PVTQPSIQIT NTTVKELGSV TLTCFSKDTG VSVRWLFNSQ 

       370        380        390        400        410        420 
SLQLTDRMTL SQDNSTLRID PIKREDAGDY QCEISNPVSF RISHPIKLDV IPDPTQGNSG 

       430        440        450        460        470        480 
LSEGAIAGIV IGSVAGVALI AALAYFLYSR KTGGGSDHRD LTEHKPSTSS HNLGPSDDSP 

       490        500        510 
NKVDDVSYSV LNFNAQQSKR PTSASSSPTE TVYSVVKKK 

« Hide

Isoform Short (C-CAM3) [UniParc].

Checksum: 0C7709DFBFA72FA0
Show »

FASTA45850,614

References

« Hide 'large scale' references
[1]"Cloning and expression of a cDNA coding for a rat liver plasma membrane ecto-ATPase. The primary structure of the ecto-ATPase is similar to that of the human biliary glycoprotein I."
Lin S.-H., Guidotti G.
J. Biol. Chem. 264:14408-14414(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG), PROTEIN SEQUENCE OF 50-68.
Strain: Sprague-Dawley.
Tissue: Liver.
[2]"Different isoforms and stock-specific variants of the cell adhesion molecule C-CAM (cell-CAM 105) in rat liver."
Edlund M., Gaardsvoll H., Bock E., Oebrink B.
Eur. J. Biochem. 213:1109-1116(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
Strain: Sprague-Dawley and Wistar.
Tissue: Liver.
[3]"Molecular cloning and expression of a new rat liver cell-CAM105 isoform. Differential phosphorylation of isoforms."
Culic O., Huang Q., Flanagan D., Hixon D., Lin S.-H.
Biochem. J. 285:47-53(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
Strain: Sprague-Dawley.
Tissue: Liver.
[4]"The cytoplasmic domain of C-CAM is required for C-CAM-mediated adhesion function: studies of a C-CAM transcript containing an unspliced intron."
Cheung P.H., Culic O., Qiu Y., Earley K., Thompson N., Hixson D.C., Lin S.-H.
Biochem. J. 295:427-435(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Intestine.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
Tissue: Prostate.
[6]"The cell adhesion molecule Cell-CAM 105 is an ecto-ATPase and a member of the immunoglobulin superfamily."
Aurivillius M., Hansen O.C., Lazrek M.B.S., Bock E., Oebrink B.
FEBS Lett. 264:267-269(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 58-66 AND 119-138.
[7]"Characterisation of the ATP-dependent taurocholate-carrier protein (gp110) of the hepatocyte canalicular membrane."
Becker A., Lucka L., Kilian C., Kannicht C., Reutter W.
Eur. J. Biochem. 214:539-548(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: PARTIAL PROTEIN SEQUENCE.
Strain: Wistar.
Tissue: Liver.
[8]"The rat liver ecto-ATPase is also a canalicular bile acid transport protein."
Sippel C.J., Suchy F.J., Ananthanarayanan M., Perlmutter D.H.
J. Biol. Chem. 268:2083-2091(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 110-138 AND 148-150, PHOSPHORYLATION.
[9]"pp120/ecto-ATPase, an endogenous substrate of the insulin receptor tyrosine kinase, is expressed as two variably spliced isoforms."
Najjae S.M., Accili D., Philippe N., Jernberg J., Margolis R., Taylor S.I.
J. Biol. Chem. 268:1201-1206(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: ALTERNATIVE SPLICING.
[10]"Immunochemical characterization of two isoforms of rat liver ecto-ATPase that show an immunological and structural identity with a glycoprotein cell-adhesion molecule with Mr 105,000."
Lin S.-H., Culic O., Flanagan D., Hixson D.C.
Biochem. J. 278:155-161(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J04963 mRNA. Translation: AAA41104.1.
X71122 mRNA. Translation: CAA50435.1.
Z12019 mRNA. Translation: CAA78054.1.
M92848 mRNA. Translation: AAA16783.1. Different termination.
BC061740 mRNA. Translation: AAH61740.1.
PIRA44783.
S23969.
RefSeqNP_001029032.1. NM_001033860.1.
NP_001029033.1. NM_001033861.1.
NP_001029034.1. NM_001033862.1.
NP_113943.1. NM_031755.2.
UniGeneRn.91235.

3D structure databases

ProteinModelPortalP16573.
SMRP16573. Positions 280-415.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-220849.

Protein family/group databases

TCDB8.A.23.1.4. the basigin (basigin) family.

PTM databases

PhosphoSiteP16573.

Proteomic databases

PaxDbP16573.
PRIDEP16573.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000051892; ENSRNOP00000046654; ENSRNOG00000020578.
GeneID81613.
KEGGrno:81613.
UCSCRGD:67396. rat. [P16573-1]

Organism-specific databases

CTD634.
RGD67396. Ceacam1.

Phylogenomic databases

eggNOGNOG149650.
GeneTreeENSGT00750000117386.
HOGENOMHOG000233417.
HOVERGENHBG007922.
InParanoidP16573.
KOK06499.
OrthoDBEOG74BJS7.
PhylomeDBP16573.
TreeFamTF336859.

Gene expression databases

GenevestigatorP16573.

Family and domain databases

Gene3D2.60.40.10. 4 hits.
InterProIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013106. Ig_V-set.
IPR013151. Immunoglobulin.
[Graphical view]
PfamPF00047. ig. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
SMARTSM00409. IG. 2 hits.
SM00408. IGc2. 2 hits.
[Graphical view]
PROSITEPS50835. IG_LIKE. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio615097.

Entry information

Entry nameCEAM1_RAT
AccessionPrimary (citable) accession number: P16573
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: October 1, 1994
Last modified: April 16, 2014
This is version 129 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families