Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P16573

- CEAM1_RAT

UniProt

P16573 - CEAM1_RAT

Protein

Carcinoembryonic antigen-related cell adhesion molecule 1

Gene

Ceacam1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 3 (01 Oct 1994)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    This protein is a calcium-independent cell adhesion molecule with homophilic binding properties. May play a role in the formation and maintenance of the specialized membrane structure of the apical surface of the hepatocytes.

    GO - Molecular functioni

    1. protein homodimerization activity Source: UniProtKB
    2. protein kinase binding Source: RGD

    GO - Biological processi

    1. cell adhesion Source: UniProtKB-KW
    2. positive regulation of MAP kinase activity Source: MGI
    3. protein homotrimerization Source: UniProtKB
    4. signal transduction Source: RGD

    Keywords - Biological processi

    Cell adhesion

    Protein family/group databases

    TCDBi8.A.23.1.4. the basigin (basigin) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Carcinoembryonic antigen-related cell adhesion molecule 1
    Alternative name(s):
    ATP-dependent taurocolate-carrier protein
    Cell-CAM 105
    Short name:
    C-CAM 105
    Ecto-ATPase
    GP110
    pp120
    CD_antigen: CD66a
    Gene namesi
    Name:Ceacam1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 1

    Organism-specific databases

    RGDi67396. Ceacam1.

    Subcellular locationi

    Cell membrane; Single-pass type I membrane protein
    Note: Canalicular domain of hepatocyte plasma membranes.

    GO - Cellular componenti

    1. integral component of plasma membrane Source: RGD

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3434By similarityAdd
    BLAST
    Chaini35 – 519485Carcinoembryonic antigen-related cell adhesion molecule 1PRO_0000014564Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei35 – 351Pyrrolidone carboxylic acidBy similarity
    Glycosylationi87 – 871N-linked (GlcNAc...)
    Glycosylationi104 – 1041N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi113 – 1131N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi148 – 1481N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi152 – 1521N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi167 ↔ 215PROSITE-ProRule annotation
    Glycosylationi172 – 1721N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi173 – 1731N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi197 – 1971N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi224 – 2241N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi256 – 2561N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi259 ↔ 299PROSITE-ProRule annotation
    Glycosylationi288 – 2881N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi292 – 2921N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi302 – 3021N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi315 – 3151N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi331 – 3311N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi344 ↔ 392PROSITE-ProRule annotation
    Glycosylationi374 – 3741N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Phosphorylated on serine residues in both isoforms. On serine and tyrosine residues in cytoplasmic region of the long isoform.1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein, Pyrrolidone carboxylic acid

    Proteomic databases

    PaxDbiP16573.
    PRIDEiP16573.

    PTM databases

    PhosphoSiteiP16573.

    Expressioni

    Tissue specificityi

    Expressed in epithelia, vessel endothelia, leukocytes and platelets.

    Gene expression databases

    GenevestigatoriP16573.

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-220849.

    Structurei

    3D structure databases

    ProteinModelPortaliP16573.
    SMRiP16573. Positions 280-415.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini35 – 422388ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini449 – 51971CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei423 – 44826HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini42 – 14099Ig-like V-typeAdd
    BLAST
    Domaini147 – 23286Ig-like C2-type 1Add
    BLAST
    Domaini237 – 31781Ig-like C2-type 2Add
    BLAST
    Domaini325 – 40379Ig-like C2-type 3Add
    BLAST

    Sequence similaritiesi

    Belongs to the immunoglobulin superfamily. CEA family.Curated

    Keywords - Domaini

    Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG149650.
    GeneTreeiENSGT00750000117386.
    HOGENOMiHOG000233417.
    HOVERGENiHBG007922.
    InParanoidiP16573.
    KOiK06499.
    OrthoDBiEOG74BJS7.
    PhylomeDBiP16573.
    TreeFamiTF336859.

    Family and domain databases

    Gene3Di2.60.40.10. 4 hits.
    InterProiIPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR003599. Ig_sub.
    IPR003598. Ig_sub2.
    IPR013106. Ig_V-set.
    IPR013151. Immunoglobulin.
    [Graphical view]
    PfamiPF00047. ig. 1 hit.
    PF07686. V-set. 1 hit.
    [Graphical view]
    SMARTiSM00409. IG. 2 hits.
    SM00408. IGc2. 2 hits.
    [Graphical view]
    PROSITEiPS50835. IG_LIKE. 3 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform Long (identifier: P16573-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MELASARLLR GQIPWRGLLL TASLLTYWSP LTTAQVTVDA VPPNVVEESS    50
    VLLLTHNLPQ EFQVFYWYKV TTTGLNSEIA RYIRSSNTSQ TEPAYSGRVT 100
    IYSNGSLFFQ NVNKTDEGPY TLSVIDKQFN PIQTSVQFRV YPALQKPNVT 150
    GNNSNPMEGE PFVSLMCEPY TNNTSYLWSR NGESLSEGDR VTFSEGNRTL 200
    TLLNVRRTDK GYYECEARNP ATFNRSDPFN LDVIYGPDAP VISPPDIYLH 250
    QGSNLNLSCH ADSNPPAQYF WLINEKLQTS SQELFISNIT TNNSGTYACF 300
    VNNTVTGLSR TTVKNITVFE PVTQPSIQIT NTTVKELGSV TLTCFSKDTG 350
    VSVRWLFNSQ SLQLTDRMTL SQDNSTLRID PIKREDAGDY QCEISNPVSF 400
    RISHPIKLDV IPDPTQGNSG LSEGAIAGIV IGSVAGVALI AALAYFLYSR 450
    KTGGGSDHRD LTEHKPSTSS HNLGPSDDSP NKVDDVSYSV LNFNAQQSKR 500
    PTSASSSPTE TVYSVVKKK 519
    Length:519
    Mass (Da):57,264
    Last modified:October 1, 1994 - v3
    Checksum:i04143928D7975776
    GO
    Isoform Short (identifier: P16573-2) [UniParc]FASTAAdd to Basket

    Also known as: C-CAM3

    The sequence of this isoform differs from the canonical sequence as follows:
         455-458: GSDH → SGSF
         459-519: Missing.

    Show »
    Length:458
    Mass (Da):50,614
    Checksum:i0C7709DFBFA72FA0
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti49 – 491S → K.
    Natural varianti55 – 551T → A.
    Natural varianti70 – 701V → G.
    Natural varianti73 – 764TGLN → LNPD.
    Natural varianti86 – 861S → D.
    Natural varianti88 – 881T → M.
    Natural varianti90 – 901Q → K.
    Natural varianti92 – 921E → G.
    Natural varianti99 – 991V → E.
    Natural varianti118 – 1192GP → RA.
    Natural varianti125 – 1251I → F.
    Natural varianti127 – 1271K → Q.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei455 – 4584GSDH → SGSF in isoform Short. 2 PublicationsVSP_002504
    Alternative sequencei459 – 51961Missing in isoform Short. 2 PublicationsVSP_002505Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J04963 mRNA. Translation: AAA41104.1.
    X71122 mRNA. Translation: CAA50435.1.
    Z12019 mRNA. Translation: CAA78054.1.
    M92848 mRNA. Translation: AAA16783.1. Different termination.
    BC061740 mRNA. Translation: AAH61740.1.
    PIRiA44783.
    S23969.
    RefSeqiNP_001029032.1. NM_001033860.1.
    NP_001029033.1. NM_001033861.1.
    NP_001029034.1. NM_001033862.1.
    NP_113943.1. NM_031755.2.
    UniGeneiRn.91235.

    Genome annotation databases

    EnsembliENSRNOT00000051892; ENSRNOP00000046654; ENSRNOG00000020578.
    GeneIDi81613.
    KEGGirno:81613.
    UCSCiRGD:67396. rat. [P16573-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J04963 mRNA. Translation: AAA41104.1 .
    X71122 mRNA. Translation: CAA50435.1 .
    Z12019 mRNA. Translation: CAA78054.1 .
    M92848 mRNA. Translation: AAA16783.1 . Different termination.
    BC061740 mRNA. Translation: AAH61740.1 .
    PIRi A44783.
    S23969.
    RefSeqi NP_001029032.1. NM_001033860.1.
    NP_001029033.1. NM_001033861.1.
    NP_001029034.1. NM_001033862.1.
    NP_113943.1. NM_031755.2.
    UniGenei Rn.91235.

    3D structure databases

    ProteinModelPortali P16573.
    SMRi P16573. Positions 280-415.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-220849.

    Protein family/group databases

    TCDBi 8.A.23.1.4. the basigin (basigin) family.

    PTM databases

    PhosphoSitei P16573.

    Proteomic databases

    PaxDbi P16573.
    PRIDEi P16573.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000051892 ; ENSRNOP00000046654 ; ENSRNOG00000020578 .
    GeneIDi 81613.
    KEGGi rno:81613.
    UCSCi RGD:67396. rat. [P16573-1 ]

    Organism-specific databases

    CTDi 634.
    RGDi 67396. Ceacam1.

    Phylogenomic databases

    eggNOGi NOG149650.
    GeneTreei ENSGT00750000117386.
    HOGENOMi HOG000233417.
    HOVERGENi HBG007922.
    InParanoidi P16573.
    KOi K06499.
    OrthoDBi EOG74BJS7.
    PhylomeDBi P16573.
    TreeFami TF336859.

    Miscellaneous databases

    NextBioi 615097.

    Gene expression databases

    Genevestigatori P16573.

    Family and domain databases

    Gene3Di 2.60.40.10. 4 hits.
    InterProi IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR003599. Ig_sub.
    IPR003598. Ig_sub2.
    IPR013106. Ig_V-set.
    IPR013151. Immunoglobulin.
    [Graphical view ]
    Pfami PF00047. ig. 1 hit.
    PF07686. V-set. 1 hit.
    [Graphical view ]
    SMARTi SM00409. IG. 2 hits.
    SM00408. IGc2. 2 hits.
    [Graphical view ]
    PROSITEi PS50835. IG_LIKE. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of a cDNA coding for a rat liver plasma membrane ecto-ATPase. The primary structure of the ecto-ATPase is similar to that of the human biliary glycoprotein I."
      Lin S.-H., Guidotti G.
      J. Biol. Chem. 264:14408-14414(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG), PROTEIN SEQUENCE OF 50-68.
      Strain: Sprague-Dawley.
      Tissue: Liver.
    2. "Different isoforms and stock-specific variants of the cell adhesion molecule C-CAM (cell-CAM 105) in rat liver."
      Edlund M., Gaardsvoll H., Bock E., Oebrink B.
      Eur. J. Biochem. 213:1109-1116(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
      Strain: Sprague-Dawley and Wistar.
      Tissue: Liver.
    3. "Molecular cloning and expression of a new rat liver cell-CAM105 isoform. Differential phosphorylation of isoforms."
      Culic O., Huang Q., Flanagan D., Hixon D., Lin S.-H.
      Biochem. J. 285:47-53(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
      Strain: Sprague-Dawley.
      Tissue: Liver.
    4. "The cytoplasmic domain of C-CAM is required for C-CAM-mediated adhesion function: studies of a C-CAM transcript containing an unspliced intron."
      Cheung P.H., Culic O., Qiu Y., Earley K., Thompson N., Hixson D.C., Lin S.-H.
      Biochem. J. 295:427-435(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Intestine.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
      Tissue: Prostate.
    6. "The cell adhesion molecule Cell-CAM 105 is an ecto-ATPase and a member of the immunoglobulin superfamily."
      Aurivillius M., Hansen O.C., Lazrek M.B.S., Bock E., Oebrink B.
      FEBS Lett. 264:267-269(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 58-66 AND 119-138.
    7. "Characterisation of the ATP-dependent taurocholate-carrier protein (gp110) of the hepatocyte canalicular membrane."
      Becker A., Lucka L., Kilian C., Kannicht C., Reutter W.
      Eur. J. Biochem. 214:539-548(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL PROTEIN SEQUENCE.
      Strain: Wistar.
      Tissue: Liver.
    8. "The rat liver ecto-ATPase is also a canalicular bile acid transport protein."
      Sippel C.J., Suchy F.J., Ananthanarayanan M., Perlmutter D.H.
      J. Biol. Chem. 268:2083-2091(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 110-138 AND 148-150, PHOSPHORYLATION.
    9. "pp120/ecto-ATPase, an endogenous substrate of the insulin receptor tyrosine kinase, is expressed as two variably spliced isoforms."
      Najjae S.M., Accili D., Philippe N., Jernberg J., Margolis R., Taylor S.I.
      J. Biol. Chem. 268:1201-1206(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: ALTERNATIVE SPLICING.
    10. "Immunochemical characterization of two isoforms of rat liver ecto-ATPase that show an immunological and structural identity with a glycoprotein cell-adhesion molecule with Mr 105,000."
      Lin S.-H., Culic O., Flanagan D., Hixson D.C.
      Biochem. J. 278:155-161(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.

    Entry informationi

    Entry nameiCEAM1_RAT
    AccessioniPrimary (citable) accession number: P16573
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 130 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3