P16573 (CEAM1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Carcinoembryonic antigen-related cell adhesion molecule 1

Alternative name(s):
ATP-dependent taurocolate-carrier protein
Cell-CAM 105
Short name=C-CAM 105
Ecto-ATPase
GP110
pp120
CD_antigen=CD66a

Gene names

Name:

Ceacam1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	519 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This protein is a calcium-independent cell adhesion molecule with homophilic binding properties. May play a role in the formation and maintenance of the specialized membrane structure of the apical surface of the hepatocytes.
Subcellular location	Cell membrane; Single-pass type I membrane protein. Note: Canalicular domain of hepatocyte plasma membranes.
Tissue specificity	Expressed in epithelia, vessel endothelia, leukocytes and platelets.
Post-translational modification	Phosphorylated on serine residues in both isoforms. On serine and tyrosine residues in cytoplasmic region of the long isoform. Ref.8
Sequence similarities	Belongs to the immunoglobulin superfamily. CEA family. Contains 3 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Immunoglobulin domain Repeat Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein Phosphoprotein Pyrrolidone carboxylic acid
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cell adhesion Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of MAP kinase activity Inferred from direct assay PubMed 11994468. Source: MGI signal transduction Inferred from direct assay PubMed 11994468. Source: RGD
Cellular_component	integral to plasma membrane Traceable author statement PubMed 11994468. Source: RGD
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Long (identifier: P16573-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: P16573-2)

Also known as: C-CAM3;

The sequence of this isoform differs from the canonical sequence as follows:
     455-458: GSDH → SGSF
     459-519: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 34	34	By similarity
	Chain	35 – 519	485	Carcinoembryonic antigen-related cell adhesion molecule 1		PRO_0000014564
Regions
	Topological domain	35 – 422	388	Extracellular Potential
	Transmembrane	423 – 448	26	Helical; Potential
	Topological domain	449 – 519	71	Cytoplasmic Potential
	Domain	42 – 140	99	Ig-like V-type
	Domain	147 – 232	86	Ig-like C2-type 1
	Domain	237 – 317	81	Ig-like C2-type 2
	Domain	325 – 403	79	Ig-like C2-type 3
Amino acid modifications
	Modified residue	35	1	Pyrrolidone carboxylic acid By similarity
	Glycosylation	87	1	N-linked (GlcNAc...)
	Glycosylation	104	1	N-linked (GlcNAc...) Potential
	Glycosylation	113	1	N-linked (GlcNAc...) Potential
	Glycosylation	148	1	N-linked (GlcNAc...) Potential
	Glycosylation	152	1	N-linked (GlcNAc...) Potential
	Glycosylation	172	1	N-linked (GlcNAc...) Potential
	Glycosylation	173	1	N-linked (GlcNAc...) Potential
	Glycosylation	197	1	N-linked (GlcNAc...) Potential
	Glycosylation	224	1	N-linked (GlcNAc...) Potential
	Glycosylation	256	1	N-linked (GlcNAc...) Potential
	Glycosylation	288	1	N-linked (GlcNAc...) Potential
	Glycosylation	292	1	N-linked (GlcNAc...) Potential
	Glycosylation	302	1	N-linked (GlcNAc...) Potential
	Glycosylation	315	1	N-linked (GlcNAc...) Potential
	Glycosylation	331	1	N-linked (GlcNAc...) Potential
	Glycosylation	374	1	N-linked (GlcNAc...) Potential
	Disulfide bond	167 ↔ 215		By similarity
	Disulfide bond	259 ↔ 299		By similarity
	Disulfide bond	344 ↔ 392		By similarity
Natural variations
	Alternative sequence	455 – 458	4	GSDH → SGSF in isoform Short.		VSP_002504
	Alternative sequence	459 – 519	61	Missing in isoform Short.		VSP_002505
	Natural variant	49	1	S → K.
	Natural variant	55	1	T → A.
	Natural variant	70	1	V → G.
	Natural variant	73 – 76	4	TGLN → LNPD.
	Natural variant	86	1	S → D.
	Natural variant	88	1	T → M.
	Natural variant	90	1	Q → K.
	Natural variant	92	1	E → G.
	Natural variant	99	1	V → E.
	Natural variant	118 – 119	2	GP → RA.
	Natural variant	125	1	I → F.
	Natural variant	127	1	K → Q.

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified October 1, 1994. Version 3. Checksum: 04143928D7975776	FASTA	519	57,264
	10 20 30 40 50 60 MELASARLLR GQIPWRGLLL TASLLTYWSP LTTAQVTVDA VPPNVVEESS VLLLTHNLPQ 70 80 90 100 110 120 EFQVFYWYKV TTTGLNSEIA RYIRSSNTSQ TEPAYSGRVT IYSNGSLFFQ NVNKTDEGPY 130 140 150 160 170 180 TLSVIDKQFN PIQTSVQFRV YPALQKPNVT GNNSNPMEGE PFVSLMCEPY TNNTSYLWSR 190 200 210 220 230 240 NGESLSEGDR VTFSEGNRTL TLLNVRRTDK GYYECEARNP ATFNRSDPFN LDVIYGPDAP 250 260 270 280 290 300 VISPPDIYLH QGSNLNLSCH ADSNPPAQYF WLINEKLQTS SQELFISNIT TNNSGTYACF 310 320 330 340 350 360 VNNTVTGLSR TTVKNITVFE PVTQPSIQIT NTTVKELGSV TLTCFSKDTG VSVRWLFNSQ 370 380 390 400 410 420 SLQLTDRMTL SQDNSTLRID PIKREDAGDY QCEISNPVSF RISHPIKLDV IPDPTQGNSG 430 440 450 460 470 480 LSEGAIAGIV IGSVAGVALI AALAYFLYSR KTGGGSDHRD LTEHKPSTSS HNLGPSDDSP 490 500 510 NKVDDVSYSV LNFNAQQSKR PTSASSSPTE TVYSVVKKK
	Isoform Short (C-CAM3) [UniParc]. Checksum: 0C7709DFBFA72FA0	FASTA	458	50,614
	10 20 30 40 50 60 MELASARLLR GQIPWRGLLL TASLLTYWSP LTTAQVTVDA VPPNVVEESS VLLLTHNLPQ 70 80 90 100 110 120 EFQVFYWYKV TTTGLNSEIA RYIRSSNTSQ TEPAYSGRVT IYSNGSLFFQ NVNKTDEGPY 130 140 150 160 170 180 TLSVIDKQFN PIQTSVQFRV YPALQKPNVT GNNSNPMEGE PFVSLMCEPY TNNTSYLWSR 190 200 210 220 230 240 NGESLSEGDR VTFSEGNRTL TLLNVRRTDK GYYECEARNP ATFNRSDPFN LDVIYGPDAP 250 260 270 280 290 300 VISPPDIYLH QGSNLNLSCH ADSNPPAQYF WLINEKLQTS SQELFISNIT TNNSGTYACF 310 320 330 340 350 360 VNNTVTGLSR TTVKNITVFE PVTQPSIQIT NTTVKELGSV TLTCFSKDTG VSVRWLFNSQ 370 380 390 400 410 420 SLQLTDRMTL SQDNSTLRID PIKREDAGDY QCEISNPVSF RISHPIKLDV IPDPTQGNSG 430 440 450 LSEGAIAGIV IGSVAGVALI AALAYFLYSR KTGGSGSF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of a cDNA coding for a rat liver plasma membrane ecto-ATPase. The primary structure of the ecto-ATPase is similar to that of the human biliary glycoprotein I." Lin S.-H., Guidotti G. J. Biol. Chem. 264:14408-14414(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG), PROTEIN SEQUENCE OF 50-68. Strain: Sprague-Dawley. Tissue: Liver.
[2]	"Different isoforms and stock-specific variants of the cell adhesion molecule C-CAM (cell-CAM 105) in rat liver." Edlund M., Gaardsvoll H., Bock E., Oebrink B. Eur. J. Biochem. 213:1109-1116(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT). Strain: Sprague-Dawley and Wistar. Tissue: Liver.
[3]	"Molecular cloning and expression of a new rat liver cell-CAM105 isoform. Differential phosphorylation of isoforms." Culic O., Huang Q., Flanagan D., Hixon D., Lin S.-H. Biochem. J. 285:47-53(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT). Strain: Sprague-Dawley. Tissue: Liver.
[4]	"The cytoplasmic domain of C-CAM is required for C-CAM-mediated adhesion function: studies of a C-CAM transcript containing an unspliced intron." Cheung P.H., Culic O., Qiu Y., Earley K., Thompson N., Hixson D.C., Lin S.-H. Biochem. J. 295:427-435(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Intestine.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG). Tissue: Prostate.
[6]	"The cell adhesion molecule Cell-CAM 105 is an ecto-ATPase and a member of the immunoglobulin superfamily." Aurivillius M., Hansen O.C., Lazrek M.B.S., Bock E., Oebrink B. FEBS Lett. 264:267-269(1990) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 58-66 AND 119-138.
[7]	"Characterisation of the ATP-dependent taurocholate-carrier protein (gp110) of the hepatocyte canalicular membrane." Becker A., Lucka L., Kilian C., Kannicht C., Reutter W. Eur. J. Biochem. 214:539-548(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE. Strain: Wistar. Tissue: Liver.
[8]	"The rat liver ecto-ATPase is also a canalicular bile acid transport protein." Sippel C.J., Suchy F.J., Ananthanarayanan M., Perlmutter D.H. J. Biol. Chem. 268:2083-2091(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 110-138 AND 148-150, PHOSPHORYLATION.
[9]	"pp120/ecto-ATPase, an endogenous substrate of the insulin receptor tyrosine kinase, is expressed as two variably spliced isoforms." Najjae S.M., Accili D., Philippe N., Jernberg J., Margolis R., Taylor S.I. J. Biol. Chem. 268:1201-1206(1993) [PubMed] [Europe PMC] [Abstract] Cited for: ALTERNATIVE SPLICING.
[10]	"Immunochemical characterization of two isoforms of rat liver ecto-ATPase that show an immunological and structural identity with a glycoprotein cell-adhesion molecule with Mr 105,000." Lin S.-H., Culic O., Flanagan D., Hixson D.C. Biochem. J. 278:155-161(1991) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J04963 mRNA. Translation: AAA41104.1. X71122 mRNA. Translation: CAA50435.1. Z12019 mRNA. Translation: CAA78054.1. M92848 mRNA. Translation: AAA16783.1. Different termination. BC061740 mRNA. Translation: AAH61740.1.
IPI	IPI00213862. IPI00231325.
PIR	A44783. S23969.
RefSeq	NP_001029032.1. NM_001033860.1. NP_001029033.1. NM_001033861.1. NP_001029034.1. NM_001033862.1. NP_113943.1. NM_031755.2.
UniGene	Rn.91235.
3D structure databases
ProteinModelPortal	P16573.
SMR	P16573. Positions 280-415.
ModBase	Search...
Protein family/group databases
TCDB	9.B.8.1.1. canalicular bile acid transporter (C-BAT) family.
PTM databases
PhosphoSite	P16573.
Proteomic databases
PaxDb	P16573.
PRIDE	P16573.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000051892; ENSRNOP00000046654; ENSRNOG00000020578.
GeneID	81613.
KEGG	rno:81613.
UCSC	RGD:67396. rat.
Organism-specific databases
CTD	634.
RGD	67396. Ceacam1.
Phylogenomic databases
eggNOG	NOG149650.
GeneTree	ENSGT00630000089675.
HOGENOM	HOG000233417.
HOVERGEN	HBG007922.
InParanoid	P16573.
KO	K06499.
OrthoDB	EOG41ZFBV.
Gene expression databases
ArrayExpress	P16573.
Genevestigator	P16573.
GermOnline	ENSRNOG00000020578. Rattus norvegicus.
Family and domain databases
Gene3D	2.60.40.10. 4 hits.
InterPro	IPR007110. Ig-like_dom. IPR013783. Ig-like_fold. IPR003599. Ig_sub. IPR003598. Ig_sub2. IPR013106. Ig_V-set. IPR013151. Immunoglobulin. [Graphical view]
Pfam	PF00047. ig. 1 hit. PF07686. V-set. 1 hit. [Graphical view]
SMART	SM00409. IG. 2 hits. SM00408. IGc2. 2 hits. [Graphical view]
PROSITE	PS50835. IG_LIKE. 3 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	615097.

Entry information

Entry name

CEAM1_RAT

Accession

Primary (citable) accession number: P16573

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	October 1, 1994
Last modified:	April 3, 2013
This is version 121 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program