P16562 (CRIS2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine-rich secretory protein 2
Short name=CRISP-2

Alternative name(s):
Cancer/testis antigen 36
Short name=CT36
Testis-specific protein TPX-1

Gene names

Name:	CRISP2
Synonyms:	GAPDL5, TPX1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	243 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May regulate some ion channels' activity and therebye regulate calcium fluxes during sperm capacitation By similarity.
Subunit structure	Interacts with NSUN4 isoform 3 By similarity.
Subcellular location	Secreted Probable.
Tissue specificity	Testis and epididymis.
Sequence similarities	Belongs to the CRISP family. Contains 1 SCP domain.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
PTM	Disulfide bond
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	extracellular space Traceable author statement Ref.2. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 21

21

Potential

Chain

22 – 243

222

Cysteine-rich secretory protein 2

PRO_0000006264

Regions

Domain

41 – 169

129

SCP

Amino acid modifications

Disulfide bond

189 ↔ 196

By similarity

Disulfide bond

192 ↔ 201

By similarity

Disulfide bond

205 ↔ 238

By similarity

Disulfide bond

214 ↔ 232

By similarity

Disulfide bond

223 ↔ 236

By similarity

Natural variations

Natural variant

131

1

N → S.
Corresponds to variant rs34457011 [ dbSNP | Ensembl ].

VAR_048832

Secondary structure

1

.

.

.

.

.

.

.

.

.

243

Helix Strand Turn

Details...

Beta strand

210 – 213

4

Helix

214 – 218

5

Turn

219 – 221

3

Beta strand

225 – 230

6

Helix

233 – 236

4

Sequences

	Sequence		Length	Mass (Da)
	P16562 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: C5FE698C449CFAD9	FASTA	243	27,259
	10 20 30 40 50 60 MALLPVLFLV TVLLPSLPAE GKDPAFTALL TTQLQVQREI VNKHNELRKA VSPPASNMLK 70 80 90 100 110 120 MEWSREVTTN AQRWANKCTL QHSDPEDRKT STRCGENLYM SSDPTSWSSA IQSWYDEILD 130 140 150 160 170 180 FVYGVGPKSP NAVVGHYTQL VWYSTYQVGC GIAYCPNQDS LKYYYVCQYC PAGNNMNRKN 190 200 210 220 230 240 TPYQQGTPCA GCPDDCDKGL CTNSCQYQDL LSNCDSLKNT AGCEHELLKE KCKATCLCEN KIY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and mapping of a testis-specific gene with sequence similarity to a sperm-coating glycoprotein gene." Kasahara M., Gutknecht J., Brew K., Spurr N., Goodfellow P.N. Genomics 5:527-534(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis.
[2]	"The human cysteine-rich secretory protein (CRISP) family. Primary structure and tissue distribution of CRISP-1, CRISP-2 and CRISP-3." Kraetzschmar J., Haendler B., Eberspaecher U., Roostermann D., Donner P., Schleuning W.-D. Eur. J. Biochem. 236:827-836(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis.
[3]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[5]	"Solution structure of RSGI RUH-032, a cysteine-rich domain of CRISP-2 from human." RIKEN structural genomics initiative (RSGI) Submitted (NOV-2005) to the PDB data bank Cited for: STRUCTURE BY NMR OF 204-239.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M25532 mRNA. Translation: AAA61220.1.
X95239 mRNA. Translation: CAA64526.1.
AL121950 Genomic DNA. Translation: CAC10520.1.
BC022011 mRNA. Translation: AAH22011.1.

IPI

IPI00029218.

PIR

B33329.

RefSeq

NP_001135879.1. NM_001142407.2.
NP_001135880.1. NM_001142408.2.
NP_001135889.1. NM_001142417.2.
NP_001135907.1. NM_001142435.2.
NP_001248751.1. NM_001261822.1.
NP_003287.1. NM_003296.3.

UniGene

Hs.2042.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2CQ7	NMR	-	A	204-239	[»]

ProteinModelPortal

P16562.

ModBase

Search...

PTM databases

PhosphoSite

P16562.

Polymorphism databases

DMDM

136111.

Proteomic databases

PaxDb

P16562.

PRIDE

P16562.

Protocols and materials databases

DNASU

7180.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000339139; ENSP00000339155; ENSG00000124490.

GeneID

7180.

KEGG

hsa:7180.

UCSC

uc003ozl.2. human.

Organism-specific databases

CTD

7180.

GeneCards

GC06M049706.

H-InvDB

HIX0165001.

HGNC

HGNC:12024. CRISP2.

HPA

HPA031248.

MIM

187430. gene.

neXtProt

NX_P16562.

PharmGKB

PA36703.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG2340.

HOGENOM

HOG000236338.

HOVERGEN

HBG004184.

OrthoDB

EOG4XKV7K.

Gene expression databases

ArrayExpress

P16562.

Bgee

P16562.

CleanEx

HS_CRISP2.

Genevestigator

P16562.

GermOnline

ENSG00000124490. Homo sapiens.

Family and domain databases

Gene3D

3.40.33.10. 1 hit.

InterPro

IPR001283. Allrgn_V5/Tpx1.
IPR018244. Allrgn_V5/Tpx1_CS.
IPR014044. CAP_domain.
IPR013871. Cysteine_rich_secretory.
[Graphical view]

PANTHER

PTHR10334. PTHR10334. 1 hit.

Pfam

PF00188. CAP. 1 hit.
PF08562. Crisp. 1 hit.
[Graphical view]

PRINTS

PR00837. V5TPXLIKE.

SMART

SM00198. SCP. 1 hit.
[Graphical view]

SUPFAM

SSF55797. SCP-like_extracellular. 1 hit.

PROSITE

PS01009. CRISP_1. 1 hit.
PS01010. CRISP_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P16562.

GenomeRNAi

7180.

NextBio

28148.

SOURCE

Search...

Entry information

Entry name

CRIS2_HUMAN

Accession

Primary (citable) accession number: P16562

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	May 1, 2013
This is version 116 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.