P16559 (TCMN_STRGA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Multifunctional cyclase-dehydratase-3-O-methyl transferase tcmN

Gene names

Name:

tcmN

Organism

Streptomyces glaucescens

Taxonomic identifier

1907 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	494 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The N-terminal domain enhances the formation of an early, partially cyclized intermediate, while the C-terminal domain catalyzes the 3-O-methylation of one or more later intermediates in the biosynthetic pathway. Catalyzes the methylation of tetracenomycin D3 (Tcm D3) to yield Tcm B3. Catalyzes as well the following side reactions: methylation of 8-O-methyl-Tcm D3 to yield Tcm E; and of 9-carboxymethyl-Tcm B3 to yield Tcm A2.
Pathway	Antibiotic biosynthesis; tetracenomycin C biosynthesis.
Domain	This multifunction enzyme is comprised of two structurally and functionally independent domains.

Ontologies

Keywords
Biological process	Antibiotic biosynthesis
Molecular function	Methyltransferase Transferase
Technical term	3D-structure Multifunctional enzyme
Gene Ontology (GO)
Biological process	antibiotic biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	O-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 494

494

Multifunctional cyclase-dehydratase-3-O-methyl transferase tcmN

PRO_0000072456

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

494

Helix Strand Turn

Details...

Beta strand

3 – 13

11

Helix

15 – 22

8

Helix

25 – 27

3

Helix

28 – 31

4

Beta strand

33 – 42

10

Beta strand

48 – 54

7

Beta strand

64 – 72

9

Turn

73 – 76

4

Beta strand

77 – 84

8

Beta strand

88 – 100

13

Beta strand

103 – 114

12

Helix

122 – 150

29

Sequences

	Sequence		Length	Mass (Da)
	P16559 [UniParc]. Last modified February 1, 1995. Version 2. Checksum: 6E153D32782F66B5	FASTA	494	55,930
	10 20 30 40 50 60 MAARTDNSIV VNAPFELVWD VTNDIEAWPE LFSEYAEAEI LRQDGDGFDF RLKTRPDANG 70 80 90 100 110 120 RVWEWVSHRV PDKGSRTVRA HRVETGPFAY MNLHWTYRAV AGGTEMRWVQ EFDMKPGAPF 130 140 150 160 170 180 DNAHMTAHLN TTTRANMERI KKIIEDRHRE GQRTPASVLP TELHAQQLLL LAASGRLARI 190 200 210 220 230 240 VHVLTELRIA DLLADGPRHV AELAKETDTH ELSLYRVLRS AASVGVFAEG PVRTFSATPL 250 260 270 280 290 300 SDGLRTGNPD GVLPLVKYNN MELTRRPYDE IMHSVRTGEP AFRRVFGSSF FEHLEANPEA 310 320 330 340 350 360 GEFFERFMAH WSRRLVLDGL ADQGMERFSR IADLGGGDGW FLAQILRRHP HATGLLMDLP 370 380 390 400 410 420 RVAASAGPVL EEAKVADRVT VLPGDFFTDP VPTGYDAYLF KGVLHNWSDE RAVTVLRRVR 430 440 450 460 470 480 EAIGDDDARL LIFDQVMAPE NEWDHAKLLD IDMLVLFGGR ERVLAEWRQL LLEADFDIVN 490 TPSHTWTTLE CRPV

References

[1]	"Nucleotide sequence of the tcmII-tcmIV region of the tetracenomycin C biosynthetic gene cluster of Streptomyces glaucescens and evidence that the tcmN gene encodes a multifunctional cyclase-dehydratase-O-methyl transferase." Summers R.G., Wendt-Pienkowski E., Motamedi H., Hutchinson C.R. J. Bacteriol. 174:1810-1820(1992) [PubMed: 1548230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 40716 / ETH 22794 / GLA.0.
[2]	"Analysis of the nucleotide sequence of the Streptomyces glaucescens tcmI genes provides key information about the enzymology of polyketide antibiotic biosynthesis." Bibb M.J., Biro S., Motamedi H., Collins J.F., Hutchinson C.R. EMBO J. 8:2727-2736(1989) [PubMed: 2684656] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-17. Strain: DSM 40716 / ETH 22794 / GLA.0.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M80674 Genomic DNA. Translation: AAA67518.1.
X15312 Genomic DNA. Translation: CAB38457.1.

PIR

S27696. B42276.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2RER	X-ray	1.90	A	1-170	[»]
2RES	X-ray	2.20	A	1-170	[»]
2REZ	X-ray	1.95	A	1-154	[»]

ProteinModelPortal

P16559.

SMR

P16559. Positions 1-155.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR001077. O_MeTrfase_2.
IPR005031. Polyket_cyc.
IPR023393. START-like_dom.
IPR011991. WHTH_trsnscrt_rep_DNA-bd.
[Graphical view]

Gene3D

G3DSA:3.30.530.20. G3DSA:3.30.530.20. 1 hit.
G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.

Pfam

PF00891. Methyltransf_2. 1 hit.
PF03364. Polyketide_cyc. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

TCMN_STRGA

Accession

Primary (citable) accession number: P16559

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	February 1, 1995
Last modified:	September 21, 2011
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program