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Protein

Protein numb

Gene

numb

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required in determination of cell fate during sensory organ formation in embryos. Functions in nuclei and seems to interact with nucleic acids.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi22 – 29ATPSequence analysis8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • Notch binding Source: FlyBase

GO - Biological processi

  • asymmetric cell division Source: FlyBase
  • asymmetric neuroblast division Source: FlyBase
  • cell fate commitment Source: FlyBase
  • cell fate determination Source: FlyBase
  • central nervous system development Source: FlyBase
  • centrosome localization Source: FlyBase
  • embryonic heart tube development Source: FlyBase
  • glial cell migration Source: FlyBase
  • heart development Source: FlyBase
  • Malpighian tubule tip cell differentiation Source: FlyBase
  • muscle cell fate specification Source: FlyBase
  • negative regulation of Notch signaling pathway Source: FlyBase
  • neuroblast fate determination Source: FlyBase
  • neuroblast proliferation Source: FlyBase
  • Notch signaling pathway Source: FlyBase
  • pericardial nephrocyte differentiation Source: FlyBase
  • peripheral nervous system development Source: FlyBase
  • positive regulation of endocytosis Source: FlyBase
  • protein localization Source: FlyBase
  • regulation of asymmetric cell division Source: FlyBase
  • regulation of nervous system development Source: FlyBase
  • regulation of neurogenesis Source: FlyBase
  • regulation of Notch signaling pathway Source: FlyBase
  • rhythmic behavior Source: FlyBase
  • sensory organ precursor cell division Source: FlyBase
  • sensory organ precursor cell fate determination Source: FlyBase
  • ventral cord development Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-5610780. Degradation of GLI1 by the proteasome.
R-DME-5632684. Hedgehog 'on' state.
SignaLinkiP16554.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein numb
Gene namesi
Name:numb
ORF Names:CG3779
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0002973. numb.

Subcellular locationi

GO - Cellular componenti

  • basal cortex Source: FlyBase
  • basal part of cell Source: FlyBase
  • cell cortex Source: FlyBase
  • cytoplasm Source: FlyBase
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi144I → A: Loss of binding affinity to Nak and drop in binding to single helical peptide. 1 Publication1
Mutagenesisi147V → A: Loss of binding affinity to Nak and drop in binding to single helical peptide. 1 Publication1
Mutagenesisi149F → V: Loss of binding affinity to Nak and single helical peptide. 1 Publication1
Mutagenesisi195F → V: Loss of binding affinity to Nak and single helical peptide. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000218691 – 556Protein numbAdd BLAST556

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei430Phosphothreonine1 Publication1
Modified residuei537Phosphoserine1 Publication1
Modified residuei543Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP16554.
PRIDEiP16554.

PTM databases

iPTMnetiP16554.

Expressioni

Gene expression databases

BgeeiFBgn0002973.
ExpressionAtlasiP16554. baseline.
GenevisibleiP16554. DM.

Interactioni

Subunit structurei

Interacts with Nak.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PRKCZQ055132EBI-429581,EBI-295351From a different organism.

GO - Molecular functioni

  • Notch binding Source: FlyBase

Protein-protein interaction databases

BioGridi60366. 16 interactors.
IntActiP16554. 8 interactors.
MINTiMINT-762140.
STRINGi7227.FBpp0303726.

Structurei

Secondary structure

1556
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni66 – 68Combined sources3
Helixi69 – 72Combined sources4
Helixi75 – 78Combined sources4
Beta strandi84 – 86Combined sources3
Beta strandi89 – 92Combined sources4
Beta strandi97 – 99Combined sources3
Helixi101 – 111Combined sources11
Beta strandi112 – 114Combined sources3
Beta strandi118 – 121Combined sources4
Beta strandi130 – 135Combined sources6
Turni144 – 146Combined sources3
Beta strandi147 – 151Combined sources5
Beta strandi154 – 157Combined sources4
Beta strandi159 – 164Combined sources6
Beta strandi166 – 171Combined sources6
Beta strandi173 – 181Combined sources9
Beta strandi183 – 187Combined sources5
Helixi188 – 198Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DDMNMR-A67-201[»]
2NMBNMR-A58-205[»]
ProteinModelPortaliP16554.
SMRiP16554.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP16554.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini81 – 208PIDPROSITE-ProRule annotationAdd BLAST128

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi25 – 57Arg/Lys-rich (basic)Add BLAST33

Domaini

PTB domain recognizes multiple ligands by engaging different amounts of surface area dictated by tertiary contacts rather than primary sequence. This may allow interactions with a diverse set of proteins during asymmetric division and specification of cell fate.

Sequence similaritiesi

Contains 1 PID domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3537. Eukaryota.
ENOG410XT15. LUCA.
GeneTreeiENSGT00530000062937.
InParanoidiP16554.
KOiK06057.
OMAiPKKMDRL.
OrthoDBiEOG091G0FJH.
PhylomeDBiP16554.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR010449. Numb_domain.
IPR011993. PH_dom-like.
IPR006020. PTB/PI_dom.
[Graphical view]
PfamiPF06311. NumbF. 1 hit.
PF00640. PID. 1 hit.
[Graphical view]
SMARTiSM00462. PTB. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
PROSITEiPS01179. PID. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform Zygotic (identifier: P16554-1) [UniParc]FASTAAdd to basket
Also known as: A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGNSSSHTHE PLERGFTRGK FGDVKNGKSA SFRFSKKSPK KMDRLRRSFR
60 70 80 90 100
DSFRRRKDRV PESSKPHQWQ ADEEAVRSAT CSFSVKYLGC VEVFESRGMQ
110 120 130 140 150
VCEEALKVLR QSRRRPVRGL LHVSGDGLRV VDDETKGLIV DQTIEKVSFC
160 170 180 190 200
APDRNHERGF SYICRDGTTR RWMCHGFLAC KDSGERLSHA VGCAFAVCLE
210 220 230 240 250
RKQRRDKECG VTMTFDTKNS TFTRTGSFRQ QTLTERLAMA TVGTNERSVD
260 270 280 290 300
GPGSAMPGPP AATVKPFNPF AIERPHATPN MLERQSSFRL STIGSQSPFK
310 320 330 340 350
RQMSLRINDL PSNADRQRAF LTAAAGNPMQ TPLRSVSPIA EVSPAKSAGA
360 370 380 390 400
DPLSAAAVAA DSVSQLCQEL SQGLSLLTQT DALLAAGEDL NFNNNRSINQ
410 420 430 440 450
NIIAAEKQVQ HVHSYAPPTA QVTPRVASTT PTYQTLHSQS PSRSEQSIET
460 470 480 490 500
STELPNAEQW LGHVVRSTSP AAPKRPTYLA NVGRAQTLAS GTGAAVGGGG
510 520 530 540 550
PDDPFDAEWV ANVAAAKQLS PDLPIPSTAR SPLARHSTNP FISPPKAPAQ

SFQVQL
Length:556
Mass (Da):60,614
Last modified:August 31, 2004 - v2
Checksum:i91B26959B5DE8405
GO
Isoform Maternal (identifier: P16554-2) [UniParc]FASTAAdd to basket
Also known as: B

The sequence of this isoform differs from the canonical sequence as follows:
     1-41: Missing.

Note: No experimental confirmation available.
Show »
Length:515
Mass (Da):56,078
Checksum:i135B5B5282F0E519
GO

Sequence cautioni

The sequence AAK93152 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti322T → A in AAA28730 (PubMed:2752427).Curated1
Sequence conflicti326G → D in AAA28730 (PubMed:2752427).Curated1
Sequence conflicti329M → L in AAA28730 (PubMed:2752427).Curated1
Sequence conflicti353L → S in AAA28730 (PubMed:2752427).Curated1
Sequence conflicti386A → T in AAA28730 (PubMed:2752427).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0187871 – 41Missing in isoform Maternal. 2 PublicationsAdd BLAST41

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27815 mRNA. Translation: AAA28730.1.
AE014134 Genomic DNA. Translation: AAF52776.1.
AE014134 Genomic DNA. Translation: AAN10693.1.
BT056305 mRNA. Translation: ACL68752.1.
BT056311 mRNA. Translation: ACL68758.1.
AY051728 mRNA. Translation: AAK93152.1. Different initiation.
PIRiA32466.
RefSeqiNP_001260291.1. NM_001273362.1. [P16554-1]
NP_523523.2. NM_078799.3. [P16554-1]
NP_723460.1. NM_164855.2. [P16554-2]
UniGeneiDm.4492.

Genome annotation databases

EnsemblMetazoaiFBtr0079822; FBpp0079420; FBgn0002973. [P16554-1]
FBtr0339526; FBpp0308609; FBgn0002973. [P16554-1]
GeneIDi34263.
KEGGidme:Dmel_CG3779.
UCSCiCG3779-RA. d. melanogaster. [P16554-1]

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27815 mRNA. Translation: AAA28730.1.
AE014134 Genomic DNA. Translation: AAF52776.1.
AE014134 Genomic DNA. Translation: AAN10693.1.
BT056305 mRNA. Translation: ACL68752.1.
BT056311 mRNA. Translation: ACL68758.1.
AY051728 mRNA. Translation: AAK93152.1. Different initiation.
PIRiA32466.
RefSeqiNP_001260291.1. NM_001273362.1. [P16554-1]
NP_523523.2. NM_078799.3. [P16554-1]
NP_723460.1. NM_164855.2. [P16554-2]
UniGeneiDm.4492.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DDMNMR-A67-201[»]
2NMBNMR-A58-205[»]
ProteinModelPortaliP16554.
SMRiP16554.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi60366. 16 interactors.
IntActiP16554. 8 interactors.
MINTiMINT-762140.
STRINGi7227.FBpp0303726.

PTM databases

iPTMnetiP16554.

Proteomic databases

PaxDbiP16554.
PRIDEiP16554.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0079822; FBpp0079420; FBgn0002973. [P16554-1]
FBtr0339526; FBpp0308609; FBgn0002973. [P16554-1]
GeneIDi34263.
KEGGidme:Dmel_CG3779.
UCSCiCG3779-RA. d. melanogaster. [P16554-1]

Organism-specific databases

CTDi8650.
FlyBaseiFBgn0002973. numb.

Phylogenomic databases

eggNOGiKOG3537. Eukaryota.
ENOG410XT15. LUCA.
GeneTreeiENSGT00530000062937.
InParanoidiP16554.
KOiK06057.
OMAiPKKMDRL.
OrthoDBiEOG091G0FJH.
PhylomeDBiP16554.

Enzyme and pathway databases

ReactomeiR-DME-5610780. Degradation of GLI1 by the proteasome.
R-DME-5632684. Hedgehog 'on' state.
SignaLinkiP16554.

Miscellaneous databases

EvolutionaryTraceiP16554.
GenomeRNAii34263.
PROiP16554.

Gene expression databases

BgeeiFBgn0002973.
ExpressionAtlasiP16554. baseline.
GenevisibleiP16554. DM.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR010449. Numb_domain.
IPR011993. PH_dom-like.
IPR006020. PTB/PI_dom.
[Graphical view]
PfamiPF06311. NumbF. 1 hit.
PF00640. PID. 1 hit.
[Graphical view]
SMARTiSM00462. PTB. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
PROSITEiPS01179. PID. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNUMB_DROME
AccessioniPrimary (citable) accession number: P16554
Secondary accession number(s): B8A413
, B8A419, Q8IPE9, Q961A6, Q9VLB8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 31, 2004
Last modified: November 30, 2016
This is version 160 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.