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P16553 (RAFD_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Last modified September 21, 2011. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Raffinose invertase
Short name=Invertase
EC=3.2.1.26
Gene names
Name:rafD
Encoded onPlasmid pRSD2
OrganismEscherichia coli
Taxonomic identifier562 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length476 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

May prevent the potential hasard of excessive sucrose accumulation.

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.

Subunit structure

Homodimer.

Sequence similarities

Belongs to the glycosyl hydrolase 32 family.

Ontologies

Keywords
   Molecular functionGlycosidase
Hydrolase
   Technical termPlasmid
Gene Ontology (GO)
   Biological processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: InterPro

   Molecular functionsucrose alpha-glucosidase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 476476Raffinose invertase
PRO_0000169869

Regions

Region35 – 384Substrate binding By similarity
Region97 – 982Substrate binding By similarity
Region159 – 1602Substrate binding By similarity

Sites

Active site381 By similarity
Binding site541Substrate By similarity
Binding site2141Substrate By similarity
Binding site2971Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
P16553 [UniParc].

Last modified August 1, 1990. Version 1.
Checksum: 59DF112E9A63723C

FASTA47654,327
        10         20         30         40         50         60 
MKQRLSLAQS ALEKLSARRG NTWYPIFHLA PPAGWMNDPN GLIYFNGRYH AFFQHHPASA 

        70         80         90        100        110        120 
YQGPMHWGHA TSTDMLHWQH ELVALAPGDK YDRDGCFSGS AVDDDGVLSL IYTGHICLED 

       130        140        150        160        170        180 
RGNDSIIREV QCLATSHDGI RFEKQGCVLT PPEGIMHFRD PKVWHEDGSW WMVIGARDAS 

       190        200        210        220        230        240 
DNGQVLLYRG TSLRDWHLEH VLAHSAAGES YMWECPDFFR CGNFHWLMFS PQGMNPSGYR 

       250        260        270        280        290        300 
FRNLFQSGVL AGNWKPGSVF ALKGVFEELD YGHDFYAPQS MLAEDGRRII MAWMNMWDSP 

       310        320        330        340        350        360 
VPTRSEAWAG CLTLPREVFE RDGRLCQRPV REVESLRRKC QPLSPVRLHG VQLLTENVQA 

       370        380        390        400        410        420 
AELLVTWHTV DSHAEHYGIR LGEGLRFYVD NQAGRLILWR YYPEEGLDGY RSVELPDTEY 

       430        440        450        460        470 
LTLRIFLDRS SVEVFVNDGE ATLSSRIYPQ ADSRQLSLYA AHGDAILTDG TLWMLT

« Hide

References

[1]"Nucleotide sequences and operon structure of plasmid-borne genes mediating uptake and utilization of raffinose in Escherichia coli."
Aslanidis C., Schmid K., Schmitt R.
J. Bacteriol. 171:6753-6763(1989) [PubMed: 2556373] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M27273 Genomic DNA. Translation: AAA24499.1.
PIRC43717.

3D structure databases

ProteinModelPortalP16553.
ModBaseSearch...

Protein family/group databases

CAZyGH32. Glycoside Hydrolase Family 32.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR008985. ConA-like_lec_gl.
IPR001362. Glyco_hydro_32.
IPR018053. Glyco_hydro_32_AS.
IPR013189. Glyco_hydro_32_C.
IPR013148. Glyco_hydro_32_N.
IPR023296. Glyco_hydro_43_beta-prop.
IPR006232. Suc6P_hydrolase.
[Graphical view]
Gene3DG3DSA:2.115.10.20. Glyco_hydro_43_beta-prop. 1 hit.
PfamPF08244. Glyco_hydro_32C. 1 hit.
PF00251. Glyco_hydro_32N. 1 hit.
[Graphical view]
SMARTSM00640. Glyco_32. 1 hit.
[Graphical view]
SUPFAMSSF49899. ConA_like_lec_gl. 1 hit.
SSF75005. Glyco_hydro_43_beta-prop. 1 hit.
TIGRFAMsTIGR01322. ScrB_fam. 1 hit.
PROSITEPS00609. GLYCOSYL_HYDROL_F32. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRAFD_ECOLX
AccessionPrimary (citable) accession number: P16553
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: September 21, 2011
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

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