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P16551 (RAFA_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-galactosidase

EC=3.2.1.22
Alternative name(s):
Melibiase
Gene names
Name:rafA
Encoded onPlasmid pRSD2
OrganismEscherichia coli
Taxonomic identifier562 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length708 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.

Subunit structure

Homotetramer.

Sequence similarities

Belongs to the glycosyl hydrolase 36 family.

Ontologies

Keywords
   Molecular functionGlycosidase
Hydrolase
   Technical termDirect protein sequencing
Plasmid
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functionraffinose alpha-galactosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 708708Alpha-galactosidase
PRO_0000134873

Sites

Active site4411Nucleophile By similarity
Active site4691Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
P16551 [UniParc].

Last modified August 1, 1990. Version 1.
Checksum: 3D915DA5DB0A202E

FASTA70881,188
        10         20         30         40         50         60 
MISKYCRLSS PRSDLIIKTH PHAEIIWWGS ALKHFSPDDC ASLERPVANG RLDIDTPLTL 

        70         80         90        100        110        120 
IAENALGLFS SPGLEGHRNG LDASPVFYTV DVEHTENTLR LTSEDSVAGL RLVSELVMTP 

       130        140        150        160        170        180 
SGILKVRHAL TNLREGDWQI NRFAITLPVA ERAEEVMAFH GRWTREFQPH RVRLTHDAFV 

       190        200        210        220        230        240 
LENRRGRTSH EHFPALIVGT PGFSEQQGEV WAVHLGWSGN HRMRCEAKTD GRRYVQAEAL 

       250        260        270        280        290        300 
WMPGEKALRK NETLYTPWLY ACHSADGLNG MSQQYHRFLR DEIIRFPEQK LRPVHLNTWE 

       310        320        330        340        350        360 
GIYFNHNPDY IMQMAERAAA LGVERFIIDD GWFKGRNDDR AALGDWYTDE QKYPNGLMPV 

       370        380        390        400        410        420 
INHVKSLGME FGIWVEPEMI NPDSDLFRLH PDWILSMPGY SQPTGRYQYV LNLNIPEAFD 

       430        440        450        460        470        480 
YIYKRFLWLL GEHPVDYVKW DMNRELVQAG HEGRAAADAQ TRQFYRLLDL LRERFPHVEF 

       490        500        510        520        530        540 
ESCASGGGRI DFEVLKRTHR FWASDNNDAL ERCTIQRGMS YFFPPEVMGA HIGHRRCHAT 

       550        560        570        580        590        600 
FRQHSIAFRG LTALFGHMGL ELDPVAADAK ESDGYRRYAL LYKEWRQLIH TGVLWRVDMP 

       610        620        630        640        650        660 
DSSIQVQGVV SPDQSQALFM ISQLAMPDYT LPGILRFPGL AAEVRYRLRV IDHPEIQLVG 

       670        680        690        700 
EGGHTMRRLP AWMNQPLEAS GEWLAKGGIQ LPVLDPESAI LIALERAV 

« Hide

References

[1]"Nucleotide sequences and operon structure of plasmid-borne genes mediating uptake and utilization of raffinose in Escherichia coli."
Aslanidis C., Schmid K., Schmitt R.
J. Bacteriol. 171:6753-6763(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Regulatory elements of the raffinose operon: nucleotide sequences of operator and repressor genes."
Aslanidis C., Schmitt R.
J. Bacteriol. 172:2178-2180(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-6.
[3]"Raffinose metabolism in Escherichia coli K12. Purification and properties of a new alpha-galactosidase specified by a transmissible plasmid."
Schmid K., Schmitt R.
Eur. J. Biochem. 67:95-104(1976) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-5.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M27273 Genomic DNA. Translation: AAA24497.1.
M29849 Genomic DNA. Translation: AAA24501.1.
PIRA43717.

3D structure databases

ProteinModelPortalP16551.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH36. Glycoside Hydrolase Family 36.

Proteomic databases

PRIDEP16551.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.20.20.70. 1 hit.
InterProIPR013785. Aldolase_TIM.
IPR002252. Glyco_hydro_36.
IPR000111. Glyco_hydro_GHD.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF02065. Melibiase. 1 hit.
[Graphical view]
PIRSFPIRSF005536. Agal. 1 hit.
PRINTSPR00743. GLHYDRLASE36.
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00512. ALPHA_GALACTOSIDASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRAFA_ECOLX
AccessionPrimary (citable) accession number: P16551
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: October 16, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries