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Protein

Alpha-galactosidase

Gene

rafA

Organism
Escherichia coli
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei441 – 4411NucleophileBy similarity
Active sitei469 – 4691Proton donorBy similarity

GO - Molecular functioni

  1. raffinose alpha-galactosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH36. Glycoside Hydrolase Family 36.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-galactosidase (EC:3.2.1.22)
Alternative name(s):
Melibiase
Gene namesi
Name:rafA
Encoded oniPlasmid pRSD20 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 708708Alpha-galactosidasePRO_0000134873Add
BLAST

Proteomic databases

PRIDEiP16551.

Interactioni

Subunit structurei

Homotetramer.

Structurei

3D structure databases

ProteinModelPortaliP16551.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 36 family.Curated

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR002252. Glyco_hydro_36.
IPR000111. Glyco_hydro_GHD.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02065. Melibiase. 1 hit.
[Graphical view]
PIRSFiPIRSF005536. Agal. 1 hit.
PRINTSiPR00743. GLHYDRLASE36.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00512. ALPHA_GALACTOSIDASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P16551-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISKYCRLSS PRSDLIIKTH PHAEIIWWGS ALKHFSPDDC ASLERPVANG
60 70 80 90 100
RLDIDTPLTL IAENALGLFS SPGLEGHRNG LDASPVFYTV DVEHTENTLR
110 120 130 140 150
LTSEDSVAGL RLVSELVMTP SGILKVRHAL TNLREGDWQI NRFAITLPVA
160 170 180 190 200
ERAEEVMAFH GRWTREFQPH RVRLTHDAFV LENRRGRTSH EHFPALIVGT
210 220 230 240 250
PGFSEQQGEV WAVHLGWSGN HRMRCEAKTD GRRYVQAEAL WMPGEKALRK
260 270 280 290 300
NETLYTPWLY ACHSADGLNG MSQQYHRFLR DEIIRFPEQK LRPVHLNTWE
310 320 330 340 350
GIYFNHNPDY IMQMAERAAA LGVERFIIDD GWFKGRNDDR AALGDWYTDE
360 370 380 390 400
QKYPNGLMPV INHVKSLGME FGIWVEPEMI NPDSDLFRLH PDWILSMPGY
410 420 430 440 450
SQPTGRYQYV LNLNIPEAFD YIYKRFLWLL GEHPVDYVKW DMNRELVQAG
460 470 480 490 500
HEGRAAADAQ TRQFYRLLDL LRERFPHVEF ESCASGGGRI DFEVLKRTHR
510 520 530 540 550
FWASDNNDAL ERCTIQRGMS YFFPPEVMGA HIGHRRCHAT FRQHSIAFRG
560 570 580 590 600
LTALFGHMGL ELDPVAADAK ESDGYRRYAL LYKEWRQLIH TGVLWRVDMP
610 620 630 640 650
DSSIQVQGVV SPDQSQALFM ISQLAMPDYT LPGILRFPGL AAEVRYRLRV
660 670 680 690 700
IDHPEIQLVG EGGHTMRRLP AWMNQPLEAS GEWLAKGGIQ LPVLDPESAI

LIALERAV
Length:708
Mass (Da):81,188
Last modified:August 1, 1990 - v1
Checksum:i3D915DA5DB0A202E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27273 Genomic DNA. Translation: AAA24497.1.
M29849 Genomic DNA. Translation: AAA24501.1.
PIRiA43717.
RefSeqiWP_000624161.1. NZ_JNBX01000073.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27273 Genomic DNA. Translation: AAA24497.1.
M29849 Genomic DNA. Translation: AAA24501.1.
PIRiA43717.
RefSeqiWP_000624161.1. NZ_JNBX01000073.1.

3D structure databases

ProteinModelPortaliP16551.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH36. Glycoside Hydrolase Family 36.

Proteomic databases

PRIDEiP16551.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR002252. Glyco_hydro_36.
IPR000111. Glyco_hydro_GHD.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02065. Melibiase. 1 hit.
[Graphical view]
PIRSFiPIRSF005536. Agal. 1 hit.
PRINTSiPR00743. GLHYDRLASE36.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00512. ALPHA_GALACTOSIDASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequences and operon structure of plasmid-borne genes mediating uptake and utilization of raffinose in Escherichia coli."
    Aslanidis C., Schmid K., Schmitt R.
    J. Bacteriol. 171:6753-6763(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Regulatory elements of the raffinose operon: nucleotide sequences of operator and repressor genes."
    Aslanidis C., Schmitt R.
    J. Bacteriol. 172:2178-2180(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-6.
  3. "Raffinose metabolism in Escherichia coli K12. Purification and properties of a new alpha-galactosidase specified by a transmissible plasmid."
    Schmid K., Schmitt R.
    Eur. J. Biochem. 67:95-104(1975) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-5.

Entry informationi

Entry nameiRAFA_ECOLX
AccessioniPrimary (citable) accession number: P16551
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: January 7, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing, Plasmid

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.