Reviewed, UniProtKB/Swiss-Prot P16551 (RAFA_ECOLX)

Names and origin

Protein names

Recommended name:
    Alpha-galactosidase
    EC=3.2.1.22
Alternative name(s):
    Melibiase

Gene names

Name:

rafA

Encoded on

Plasmid pRSD2

Organism

Escherichia coli

Taxonomic identifier

562 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	708 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactohydrolase.
Subunit structure	Homotetramer.
Sequence similarities	Belongs to the glycosyl hydrolase 36 family.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Technical term	Direct protein sequencing Plasmid
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	alpha-galactosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 708	708	Alpha-galactosidase		PRO_0000134873
Sites
	Active site	441	1	Nucleophile By similarity
	Active site	469	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P16551-1 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: 3D915DA5DB0A202E	FASTA	708	81,188
	10 20 30 40 50 60 MISKYCRLSS PRSDLIIKTH PHAEIIWWGS ALKHFSPDDC ASLERPVANG RLDIDTPLTL 70 80 90 100 110 120 IAENALGLFS SPGLEGHRNG LDASPVFYTV DVEHTENTLR LTSEDSVAGL RLVSELVMTP 130 140 150 160 170 180 SGILKVRHAL TNLREGDWQI NRFAITLPVA ERAEEVMAFH GRWTREFQPH RVRLTHDAFV 190 200 210 220 230 240 LENRRGRTSH EHFPALIVGT PGFSEQQGEV WAVHLGWSGN HRMRCEAKTD GRRYVQAEAL 250 260 270 280 290 300 WMPGEKALRK NETLYTPWLY ACHSADGLNG MSQQYHRFLR DEIIRFPEQK LRPVHLNTWE 310 320 330 340 350 360 GIYFNHNPDY IMQMAERAAA LGVERFIIDD GWFKGRNDDR AALGDWYTDE QKYPNGLMPV 370 380 390 400 410 420 INHVKSLGME FGIWVEPEMI NPDSDLFRLH PDWILSMPGY SQPTGRYQYV LNLNIPEAFD 430 440 450 460 470 480 YIYKRFLWLL GEHPVDYVKW DMNRELVQAG HEGRAAADAQ TRQFYRLLDL LRERFPHVEF 490 500 510 520 530 540 ESCASGGGRI DFEVLKRTHR FWASDNNDAL ERCTIQRGMS YFFPPEVMGA HIGHRRCHAT 550 560 570 580 590 600 FRQHSIAFRG LTALFGHMGL ELDPVAADAK ESDGYRRYAL LYKEWRQLIH TGVLWRVDMP 610 620 630 640 650 660 DSSIQVQGVV SPDQSQALFM ISQLAMPDYT LPGILRFPGL AAEVRYRLRV IDHPEIQLVG 670 680 690 700 EGGHTMRRLP AWMNQPLEAS GEWLAKGGIQ LPVLDPESAI LIALERAV

References

[1]	"Nucleotide sequences and operon structure of plasmid-borne genes mediating uptake and utilization of raffinose in Escherichia coli." Aslanidis C., Schmid K., Schmitt R. J. Bacteriol. 171:6753-6763(1989) [PubMed: 2556373] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Regulatory elements of the raffinose operon: nucleotide sequences of operator and repressor genes." Aslanidis C., Schmitt R. J. Bacteriol. 172:2178-2180(1990) [PubMed: 2180920] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-6.
[3]	"Raffinose metabolism in Escherichia coli K12. Purification and properties of a new alpha-galactosidase specified by a transmissible plasmid." Schmid K., Schmitt R. Eur. J. Biochem. 67:95-104(1976) [PubMed: 786627] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M27273 Genomic DNA. Translation: AAA24497.1. M29849 Genomic DNA. Translation: AAA24501.1.
PIR	A43717.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH36. Glycoside Hydrolase Family 36.
Enzyme and pathway databases
BRENDA	3.2.1.22. 246.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR002252. Glyco_hydro_36. IPR017853. Glyco_hydro_catalytic_core. IPR000111. Glyco_hydro_GHD. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF02065. Melibiase. 1 hit. [Graphical view]
PIRSF	PIRSF005536. Agal. 1 hit.
PRINTS	PR00743. GLHYDRLASE36.
PROSITE	PS00512. ALPHA_GALACTOSIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RAFA_ECOLX

Accession

Primary (citable) accession number: P16551

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	November 24, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)