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Protein

Alpha-galactosidase

Gene

rafA

Organism
Escherichia coli
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei441NucleophileBy similarity1
Active sitei469Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase

Protein family/group databases

CAZyiGH36 Glycoside Hydrolase Family 36

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-galactosidase (EC:3.2.1.22)
Alternative name(s):
Melibiase
Gene namesi
Name:rafA
Encoded oniPlasmid pRSD20 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001348731 – 708Alpha-galactosidaseAdd BLAST708

Proteomic databases

PRIDEiP16551

Interactioni

Subunit structurei

Homotetramer.

Structurei

3D structure databases

ProteinModelPortaliP16551
SMRiP16551
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 36 family.Curated

Family and domain databases

CDDicd14791 GH36, 1 hit
Gene3Di2.70.98.60, 1 hit
3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR038417 Alpga-gal_N_sf
IPR000111 Glyco_hydro_27/36_CS
IPR002252 Glyco_hydro_36
IPR031705 Glyco_hydro_36_C
IPR031704 Glyco_hydro_36_N
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF16874 Glyco_hydro_36C, 1 hit
PF16875 Glyco_hydro_36N, 1 hit
PF02065 Melibiase, 1 hit
PIRSFiPIRSF005536 Agal, 1 hit
PRINTSiPR00743 GLHYDRLASE36
SUPFAMiSSF51445 SSF51445, 1 hit
PROSITEiView protein in PROSITE
PS00512 ALPHA_GALACTOSIDASE, 1 hit

Sequencei

Sequence statusi: Complete.

P16551-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISKYCRLSS PRSDLIIKTH PHAEIIWWGS ALKHFSPDDC ASLERPVANG
60 70 80 90 100
RLDIDTPLTL IAENALGLFS SPGLEGHRNG LDASPVFYTV DVEHTENTLR
110 120 130 140 150
LTSEDSVAGL RLVSELVMTP SGILKVRHAL TNLREGDWQI NRFAITLPVA
160 170 180 190 200
ERAEEVMAFH GRWTREFQPH RVRLTHDAFV LENRRGRTSH EHFPALIVGT
210 220 230 240 250
PGFSEQQGEV WAVHLGWSGN HRMRCEAKTD GRRYVQAEAL WMPGEKALRK
260 270 280 290 300
NETLYTPWLY ACHSADGLNG MSQQYHRFLR DEIIRFPEQK LRPVHLNTWE
310 320 330 340 350
GIYFNHNPDY IMQMAERAAA LGVERFIIDD GWFKGRNDDR AALGDWYTDE
360 370 380 390 400
QKYPNGLMPV INHVKSLGME FGIWVEPEMI NPDSDLFRLH PDWILSMPGY
410 420 430 440 450
SQPTGRYQYV LNLNIPEAFD YIYKRFLWLL GEHPVDYVKW DMNRELVQAG
460 470 480 490 500
HEGRAAADAQ TRQFYRLLDL LRERFPHVEF ESCASGGGRI DFEVLKRTHR
510 520 530 540 550
FWASDNNDAL ERCTIQRGMS YFFPPEVMGA HIGHRRCHAT FRQHSIAFRG
560 570 580 590 600
LTALFGHMGL ELDPVAADAK ESDGYRRYAL LYKEWRQLIH TGVLWRVDMP
610 620 630 640 650
DSSIQVQGVV SPDQSQALFM ISQLAMPDYT LPGILRFPGL AAEVRYRLRV
660 670 680 690 700
IDHPEIQLVG EGGHTMRRLP AWMNQPLEAS GEWLAKGGIQ LPVLDPESAI

LIALERAV
Length:708
Mass (Da):81,188
Last modified:August 1, 1990 - v1
Checksum:i3D915DA5DB0A202E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27273 Genomic DNA Translation: AAA24497.1
M29849 Genomic DNA Translation: AAA24501.1
PIRiA43717
RefSeqiWP_000624161.1, NZ_NXMG01000112.1

Genome annotation databases

PATRICifig|562.6988.peg.1499

Similar proteinsi

Entry informationi

Entry nameiRAFA_ECOLX
AccessioniPrimary (citable) accession number: P16551
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: March 28, 2018
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing, Plasmid
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Main funding by: National Institutes of Health