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P16551

- RAFA_ECOLX

UniProt

P16551 - RAFA_ECOLX

Protein

Alpha-galactosidase

Gene

rafA

Organism
Escherichia coli
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (01 Aug 1990)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei441 – 4411NucleophileBy similarity
    Active sitei469 – 4691Proton donorBy similarity

    GO - Molecular functioni

    1. raffinose alpha-galactosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Protein family/group databases

    CAZyiGH36. Glycoside Hydrolase Family 36.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-galactosidase (EC:3.2.1.22)
    Alternative name(s):
    Melibiase
    Gene namesi
    Name:rafA
    Encoded oniPlasmid pRSD20 Publication
    OrganismiEscherichia coli
    Taxonomic identifieri562 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 708708Alpha-galactosidasePRO_0000134873Add
    BLAST

    Proteomic databases

    PRIDEiP16551.

    Interactioni

    Subunit structurei

    Homotetramer.

    Structurei

    3D structure databases

    ProteinModelPortaliP16551.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 36 family.Curated

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR002252. Glyco_hydro_36.
    IPR000111. Glyco_hydro_GHD.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02065. Melibiase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005536. Agal. 1 hit.
    PRINTSiPR00743. GLHYDRLASE36.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00512. ALPHA_GALACTOSIDASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P16551-1 [UniParc]FASTAAdd to Basket

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    MISKYCRLSS PRSDLIIKTH PHAEIIWWGS ALKHFSPDDC ASLERPVANG    50
    RLDIDTPLTL IAENALGLFS SPGLEGHRNG LDASPVFYTV DVEHTENTLR 100
    LTSEDSVAGL RLVSELVMTP SGILKVRHAL TNLREGDWQI NRFAITLPVA 150
    ERAEEVMAFH GRWTREFQPH RVRLTHDAFV LENRRGRTSH EHFPALIVGT 200
    PGFSEQQGEV WAVHLGWSGN HRMRCEAKTD GRRYVQAEAL WMPGEKALRK 250
    NETLYTPWLY ACHSADGLNG MSQQYHRFLR DEIIRFPEQK LRPVHLNTWE 300
    GIYFNHNPDY IMQMAERAAA LGVERFIIDD GWFKGRNDDR AALGDWYTDE 350
    QKYPNGLMPV INHVKSLGME FGIWVEPEMI NPDSDLFRLH PDWILSMPGY 400
    SQPTGRYQYV LNLNIPEAFD YIYKRFLWLL GEHPVDYVKW DMNRELVQAG 450
    HEGRAAADAQ TRQFYRLLDL LRERFPHVEF ESCASGGGRI DFEVLKRTHR 500
    FWASDNNDAL ERCTIQRGMS YFFPPEVMGA HIGHRRCHAT FRQHSIAFRG 550
    LTALFGHMGL ELDPVAADAK ESDGYRRYAL LYKEWRQLIH TGVLWRVDMP 600
    DSSIQVQGVV SPDQSQALFM ISQLAMPDYT LPGILRFPGL AAEVRYRLRV 650
    IDHPEIQLVG EGGHTMRRLP AWMNQPLEAS GEWLAKGGIQ LPVLDPESAI 700
    LIALERAV 708
    Length:708
    Mass (Da):81,188
    Last modified:August 1, 1990 - v1
    Checksum:i3D915DA5DB0A202E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M27273 Genomic DNA. Translation: AAA24497.1.
    M29849 Genomic DNA. Translation: AAA24501.1.
    PIRiA43717.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M27273 Genomic DNA. Translation: AAA24497.1 .
    M29849 Genomic DNA. Translation: AAA24501.1 .
    PIRi A43717.

    3D structure databases

    ProteinModelPortali P16551.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH36. Glycoside Hydrolase Family 36.

    Proteomic databases

    PRIDEi P16551.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR002252. Glyco_hydro_36.
    IPR000111. Glyco_hydro_GHD.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02065. Melibiase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005536. Agal. 1 hit.
    PRINTSi PR00743. GLHYDRLASE36.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00512. ALPHA_GALACTOSIDASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequences and operon structure of plasmid-borne genes mediating uptake and utilization of raffinose in Escherichia coli."
      Aslanidis C., Schmid K., Schmitt R.
      J. Bacteriol. 171:6753-6763(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Regulatory elements of the raffinose operon: nucleotide sequences of operator and repressor genes."
      Aslanidis C., Schmitt R.
      J. Bacteriol. 172:2178-2180(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-6.
    3. "Raffinose metabolism in Escherichia coli K12. Purification and properties of a new alpha-galactosidase specified by a transmissible plasmid."
      Schmid K., Schmitt R.
      Eur. J. Biochem. 67:95-104(1976) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-5.

    Entry informationi

    Entry nameiRAFA_ECOLX
    AccessioniPrimary (citable) accession number: P16551
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: August 1, 1990
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing, Plasmid

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3