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P16551

- RAFA_ECOLX

UniProt

P16551 - RAFA_ECOLX

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Protein

Alpha-galactosidase

Gene

rafA

Organism
Escherichia coli
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei441 – 4411NucleophileBy similarity
Active sitei469 – 4691Proton donorBy similarity

GO - Molecular functioni

  1. raffinose alpha-galactosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH36. Glycoside Hydrolase Family 36.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-galactosidase (EC:3.2.1.22)
Alternative name(s):
Melibiase
Gene namesi
Name:rafA
Encoded oniPlasmid pRSD20 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 708708Alpha-galactosidasePRO_0000134873Add
BLAST

Proteomic databases

PRIDEiP16551.

Interactioni

Subunit structurei

Homotetramer.

Structurei

3D structure databases

ProteinModelPortaliP16551.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 36 family.Curated

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR002252. Glyco_hydro_36.
IPR000111. Glyco_hydro_GHD.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02065. Melibiase. 1 hit.
[Graphical view]
PIRSFiPIRSF005536. Agal. 1 hit.
PRINTSiPR00743. GLHYDRLASE36.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00512. ALPHA_GALACTOSIDASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P16551 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MISKYCRLSS PRSDLIIKTH PHAEIIWWGS ALKHFSPDDC ASLERPVANG
60 70 80 90 100
RLDIDTPLTL IAENALGLFS SPGLEGHRNG LDASPVFYTV DVEHTENTLR
110 120 130 140 150
LTSEDSVAGL RLVSELVMTP SGILKVRHAL TNLREGDWQI NRFAITLPVA
160 170 180 190 200
ERAEEVMAFH GRWTREFQPH RVRLTHDAFV LENRRGRTSH EHFPALIVGT
210 220 230 240 250
PGFSEQQGEV WAVHLGWSGN HRMRCEAKTD GRRYVQAEAL WMPGEKALRK
260 270 280 290 300
NETLYTPWLY ACHSADGLNG MSQQYHRFLR DEIIRFPEQK LRPVHLNTWE
310 320 330 340 350
GIYFNHNPDY IMQMAERAAA LGVERFIIDD GWFKGRNDDR AALGDWYTDE
360 370 380 390 400
QKYPNGLMPV INHVKSLGME FGIWVEPEMI NPDSDLFRLH PDWILSMPGY
410 420 430 440 450
SQPTGRYQYV LNLNIPEAFD YIYKRFLWLL GEHPVDYVKW DMNRELVQAG
460 470 480 490 500
HEGRAAADAQ TRQFYRLLDL LRERFPHVEF ESCASGGGRI DFEVLKRTHR
510 520 530 540 550
FWASDNNDAL ERCTIQRGMS YFFPPEVMGA HIGHRRCHAT FRQHSIAFRG
560 570 580 590 600
LTALFGHMGL ELDPVAADAK ESDGYRRYAL LYKEWRQLIH TGVLWRVDMP
610 620 630 640 650
DSSIQVQGVV SPDQSQALFM ISQLAMPDYT LPGILRFPGL AAEVRYRLRV
660 670 680 690 700
IDHPEIQLVG EGGHTMRRLP AWMNQPLEAS GEWLAKGGIQ LPVLDPESAI

LIALERAV
Length:708
Mass (Da):81,188
Last modified:August 1, 1990 - v1
Checksum:i3D915DA5DB0A202E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M27273 Genomic DNA. Translation: AAA24497.1.
M29849 Genomic DNA. Translation: AAA24501.1.
PIRiA43717.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M27273 Genomic DNA. Translation: AAA24497.1 .
M29849 Genomic DNA. Translation: AAA24501.1 .
PIRi A43717.

3D structure databases

ProteinModelPortali P16551.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH36. Glycoside Hydrolase Family 36.

Proteomic databases

PRIDEi P16551.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR002252. Glyco_hydro_36.
IPR000111. Glyco_hydro_GHD.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02065. Melibiase. 1 hit.
[Graphical view ]
PIRSFi PIRSF005536. Agal. 1 hit.
PRINTSi PR00743. GLHYDRLASE36.
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00512. ALPHA_GALACTOSIDASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequences and operon structure of plasmid-borne genes mediating uptake and utilization of raffinose in Escherichia coli."
    Aslanidis C., Schmid K., Schmitt R.
    J. Bacteriol. 171:6753-6763(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Regulatory elements of the raffinose operon: nucleotide sequences of operator and repressor genes."
    Aslanidis C., Schmitt R.
    J. Bacteriol. 172:2178-2180(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-6.
  3. "Raffinose metabolism in Escherichia coli K12. Purification and properties of a new alpha-galactosidase specified by a transmissible plasmid."
    Schmid K., Schmitt R.
    Eur. J. Biochem. 67:95-104(1976) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-5.

Entry informationi

Entry nameiRAFA_ECOLX
AccessioniPrimary (citable) accession number: P16551
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: October 1, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing, Plasmid

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3