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P16544

- ACT3_STRCO

UniProt

P16544 - ACT3_STRCO

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Protein

Putative ketoacyl reductase

Gene
actIII, SCO5086, SCBAC28G1.12c
Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei63 – 631NADP
Binding sitei144 – 1441Substrate By similarity
Active sitei157 – 1571Proton acceptor By similarity
Binding sitei161 – 1611NADP

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 3929NADPAdd
BLAST

GO - Molecular functioni

  1. oxidoreductase activity Source: UniProtKB-KW

GO - Biological processi

  1. antibiotic biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Antibiotic biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00173.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative ketoacyl reductase (EC:1.3.1.-)
Gene namesi
Name:actIII
Ordered Locus Names:SCO5086
ORF Names:SCBAC28G1.12c
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
ProteomesiUP000001973: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 261261Putative ketoacyl reductasePRO_0000054447Add
BLAST

Proteomic databases

PRIDEiP16544.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi100226.SCO5086.

Structurei

Secondary structure

1
261
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi8 – 125
Helixi17 – 2812
Beta strandi32 – 387
Helixi40 – 5213
Beta strandi57 – 615
Helixi67 – 8014
Beta strandi85 – 895
Helixi99 – 1013
Helixi104 – 11411
Helixi116 – 12611
Turni127 – 1293
Helixi131 – 1344
Beta strandi136 – 1427
Helixi145 – 1473
Helixi155 – 17521
Turni176 – 1783
Beta strandi179 – 1879
Beta strandi189 – 1924
Helixi193 – 20614
Helixi210 – 22011
Helixi229 – 24012
Helixi242 – 2443
Beta strandi251 – 2555

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1W4ZX-ray2.50A/B1-261[»]
1X7GX-ray2.30A/B1-261[»]
1X7HX-ray2.30A/B1-261[»]
1XR3X-ray2.71A/B1-261[»]
2RH4X-ray2.30A/B1-261[»]
2RHCX-ray2.10A/B1-261[»]
2RHRX-ray2.50A/B1-261[»]
3CSDX-ray2.29A/B1-261[»]
3QRWX-ray2.79A/B1-261[»]
3RI3X-ray2.29A/B1-261[»]
4DBZX-ray2.64A/B1-261[»]
4DC0X-ray2.81A/B1-261[»]
4DC1X-ray2.82A/B1-261[»]
ProteinModelPortaliP16544.
SMRiP16544. Positions 1-261.

Miscellaneous databases

EvolutionaryTraceiP16544.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1028.
KOiK12420.
OMAiANAICAE.
OrthoDBiEOG6N3CR8.
PhylomeDBiP16544.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PIRSFiPIRSF000126. 11-beta-HSD1. 1 hit.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P16544-1 [UniParc]FASTAAdd to Basket

« Hide

MATQDSEVAL VTGATSGIGL EIARRLGKEG LRVFVCARGE EGLRTTLKEL    50
REAGVEADGR TCDVRSVPEI EALVAAVVER YGPVDVLVNN AGRPGGGATA 100
ELADELWLDV VETNLTGVFR VTKQVLKAGG MLERGTGRIV NIASTGGKQG 150
VVHAAPYSAS KHGVVGFTKA LGLELARTGI TVNAVCPGFV ETPMAASVRE 200
HYSDIWEVST EEAFDRITAR VPIGRYVQPS EVAEMVAYLI GPGAAAVTAQ 250
ALNVCGGLGN Y 261
Length:261
Mass (Da):27,265
Last modified:August 1, 1990 - v1
Checksum:iB87C83F45A88B7A9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M19536 Genomic DNA. Translation: AAA26688.1.
AL939122 Genomic DNA. Translation: CAC44199.1.
PIRiJS0108. A28788.
RefSeqiNP_629236.1. NC_003888.3.

Genome annotation databases

EnsemblBacteriaiCAC44199; CAC44199; CAC44199.
GeneIDi1100527.
KEGGisco:SCO5086.
PATRICi23740012. VBIStrCoe124346_5166.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M19536 Genomic DNA. Translation: AAA26688.1 .
AL939122 Genomic DNA. Translation: CAC44199.1 .
PIRi JS0108. A28788.
RefSeqi NP_629236.1. NC_003888.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1W4Z X-ray 2.50 A/B 1-261 [» ]
1X7G X-ray 2.30 A/B 1-261 [» ]
1X7H X-ray 2.30 A/B 1-261 [» ]
1XR3 X-ray 2.71 A/B 1-261 [» ]
2RH4 X-ray 2.30 A/B 1-261 [» ]
2RHC X-ray 2.10 A/B 1-261 [» ]
2RHR X-ray 2.50 A/B 1-261 [» ]
3CSD X-ray 2.29 A/B 1-261 [» ]
3QRW X-ray 2.79 A/B 1-261 [» ]
3RI3 X-ray 2.29 A/B 1-261 [» ]
4DBZ X-ray 2.64 A/B 1-261 [» ]
4DC0 X-ray 2.81 A/B 1-261 [» ]
4DC1 X-ray 2.82 A/B 1-261 [» ]
ProteinModelPortali P16544.
SMRi P16544. Positions 1-261.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 100226.SCO5086.

Proteomic databases

PRIDEi P16544.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAC44199 ; CAC44199 ; CAC44199 .
GeneIDi 1100527.
KEGGi sco:SCO5086.
PATRICi 23740012. VBIStrCoe124346_5166.

Phylogenomic databases

eggNOGi COG1028.
KOi K12420.
OMAi ANAICAE.
OrthoDBi EOG6N3CR8.
PhylomeDBi P16544.

Enzyme and pathway databases

UniPathwayi UPA00173 .

Miscellaneous databases

EvolutionaryTracei P16544.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view ]
Pfami PF00106. adh_short. 1 hit.
[Graphical view ]
PIRSFi PIRSF000126. 11-beta-HSD1. 1 hit.
PRINTSi PR00081. GDHRDH.
PR00080. SDRFAMILY.
PROSITEi PS00061. ADH_SHORT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence, transcription and deduced function of a gene involved in polyketide antibiotic synthesis in Streptomyces coelicolor."
    Hallam S., Malpartida F., Hopwood D.
    Gene 74:305-320(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-471 / A3(2) / M145.
  3. "Structural analysis of actinorhodin polyketide ketoreductase: cofactor binding and substrate specificity."
    Korman T.P., Hill J.A., Vu T.N., Tsai S.C.
    Biochemistry 43:14529-14538(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) IN COMPLEX WITH NADPH.
  4. "The crystal structure of the actIII actinorhodin polyketide reductase: proposed mechanism for ACP and polyketide binding."
    Hadfield A.T., Limpkin C., Teartasin W., Simpson T.J., Crosby J., Crump M.P.
    Structure 12:1865-1875(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) IN COMPLEX WITH NADPH, SUBUNIT.

Entry informationi

Entry nameiACT3_STRCO
AccessioniPrimary (citable) accession number: P16544
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: May 14, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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