Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P16544

- ACT3_STRCO

UniProt

P16544 - ACT3_STRCO

Protein

Putative ketoacyl reductase

Gene

actIII

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Aug 1990)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei63 – 631NADP2 Publications
    Binding sitei144 – 1441SubstrateBy similarity
    Active sitei157 – 1571Proton acceptorPROSITE-ProRule annotation
    Binding sitei161 – 1611NADP2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi11 – 3929NADP2 PublicationsAdd
    BLAST

    GO - Molecular functioni

    1. oxidoreductase activity Source: UniProtKB-KW

    GO - Biological processi

    1. antibiotic biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Antibiotic biosynthesis

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    UniPathwayiUPA00173.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Putative ketoacyl reductase (EC:1.3.1.-)
    Gene namesi
    Name:actIII
    Ordered Locus Names:SCO5086
    ORF Names:SCBAC28G1.12c
    OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
    Taxonomic identifieri100226 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
    ProteomesiUP000001973: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 261261Putative ketoacyl reductasePRO_0000054447Add
    BLAST

    Proteomic databases

    PRIDEiP16544.

    Interactioni

    Subunit structurei

    Homotetramer.2 Publications

    Protein-protein interaction databases

    STRINGi100226.SCO5086.

    Structurei

    Secondary structure

    1
    261
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi8 – 125
    Helixi17 – 2812
    Beta strandi32 – 387
    Helixi40 – 5213
    Beta strandi57 – 615
    Helixi67 – 8014
    Beta strandi85 – 895
    Helixi99 – 1013
    Helixi104 – 11411
    Helixi116 – 12611
    Turni127 – 1293
    Helixi131 – 1344
    Beta strandi136 – 1427
    Helixi145 – 1473
    Helixi155 – 17521
    Turni176 – 1783
    Beta strandi179 – 1879
    Beta strandi189 – 1924
    Helixi193 – 20614
    Helixi210 – 22011
    Helixi229 – 24012
    Helixi242 – 2443
    Beta strandi251 – 2555

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1W4ZX-ray2.50A/B1-261[»]
    1X7GX-ray2.30A/B1-261[»]
    1X7HX-ray2.30A/B1-261[»]
    1XR3X-ray2.71A/B1-261[»]
    2RH4X-ray2.30A/B1-261[»]
    2RHCX-ray2.10A/B1-261[»]
    2RHRX-ray2.50A/B1-261[»]
    3CSDX-ray2.29A/B1-261[»]
    3QRWX-ray2.79A/B1-261[»]
    3RI3X-ray2.29A/B1-261[»]
    4DBZX-ray2.64A/B1-261[»]
    4DC0X-ray2.81A/B1-261[»]
    4DC1X-ray2.82A/B1-261[»]
    ProteinModelPortaliP16544.
    SMRiP16544. Positions 1-261.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP16544.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1028.
    KOiK12420.
    OMAiANAICAE.
    OrthoDBiEOG6N3CR8.
    PhylomeDBiP16544.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    [Graphical view]
    PfamiPF00106. adh_short. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000126. 11-beta-HSD1. 1 hit.
    PRINTSiPR00081. GDHRDH.
    PR00080. SDRFAMILY.
    PROSITEiPS00061. ADH_SHORT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P16544-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATQDSEVAL VTGATSGIGL EIARRLGKEG LRVFVCARGE EGLRTTLKEL    50
    REAGVEADGR TCDVRSVPEI EALVAAVVER YGPVDVLVNN AGRPGGGATA 100
    ELADELWLDV VETNLTGVFR VTKQVLKAGG MLERGTGRIV NIASTGGKQG 150
    VVHAAPYSAS KHGVVGFTKA LGLELARTGI TVNAVCPGFV ETPMAASVRE 200
    HYSDIWEVST EEAFDRITAR VPIGRYVQPS EVAEMVAYLI GPGAAAVTAQ 250
    ALNVCGGLGN Y 261
    Length:261
    Mass (Da):27,265
    Last modified:August 1, 1990 - v1
    Checksum:iB87C83F45A88B7A9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M19536 Genomic DNA. Translation: AAA26688.1.
    AL939122 Genomic DNA. Translation: CAC44199.1.
    PIRiJS0108. A28788.
    RefSeqiNP_629236.1. NC_003888.3.

    Genome annotation databases

    EnsemblBacteriaiCAC44199; CAC44199; CAC44199.
    GeneIDi1100527.
    KEGGisco:SCO5086.
    PATRICi23740012. VBIStrCoe124346_5166.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M19536 Genomic DNA. Translation: AAA26688.1 .
    AL939122 Genomic DNA. Translation: CAC44199.1 .
    PIRi JS0108. A28788.
    RefSeqi NP_629236.1. NC_003888.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1W4Z X-ray 2.50 A/B 1-261 [» ]
    1X7G X-ray 2.30 A/B 1-261 [» ]
    1X7H X-ray 2.30 A/B 1-261 [» ]
    1XR3 X-ray 2.71 A/B 1-261 [» ]
    2RH4 X-ray 2.30 A/B 1-261 [» ]
    2RHC X-ray 2.10 A/B 1-261 [» ]
    2RHR X-ray 2.50 A/B 1-261 [» ]
    3CSD X-ray 2.29 A/B 1-261 [» ]
    3QRW X-ray 2.79 A/B 1-261 [» ]
    3RI3 X-ray 2.29 A/B 1-261 [» ]
    4DBZ X-ray 2.64 A/B 1-261 [» ]
    4DC0 X-ray 2.81 A/B 1-261 [» ]
    4DC1 X-ray 2.82 A/B 1-261 [» ]
    ProteinModelPortali P16544.
    SMRi P16544. Positions 1-261.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 100226.SCO5086.

    Proteomic databases

    PRIDEi P16544.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAC44199 ; CAC44199 ; CAC44199 .
    GeneIDi 1100527.
    KEGGi sco:SCO5086.
    PATRICi 23740012. VBIStrCoe124346_5166.

    Phylogenomic databases

    eggNOGi COG1028.
    KOi K12420.
    OMAi ANAICAE.
    OrthoDBi EOG6N3CR8.
    PhylomeDBi P16544.

    Enzyme and pathway databases

    UniPathwayi UPA00173 .

    Miscellaneous databases

    EvolutionaryTracei P16544.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    [Graphical view ]
    Pfami PF00106. adh_short. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000126. 11-beta-HSD1. 1 hit.
    PRINTSi PR00081. GDHRDH.
    PR00080. SDRFAMILY.
    PROSITEi PS00061. ADH_SHORT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence, transcription and deduced function of a gene involved in polyketide antibiotic synthesis in Streptomyces coelicolor."
      Hallam S., Malpartida F., Hopwood D.
      Gene 74:305-320(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-471 / A3(2) / M145.
    3. "Structural analysis of actinorhodin polyketide ketoreductase: cofactor binding and substrate specificity."
      Korman T.P., Hill J.A., Vu T.N., Tsai S.C.
      Biochemistry 43:14529-14538(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) IN COMPLEX WITH NADPH.
    4. "The crystal structure of the actIII actinorhodin polyketide reductase: proposed mechanism for ACP and polyketide binding."
      Hadfield A.T., Limpkin C., Teartasin W., Simpson T.J., Crosby J., Crump M.P.
      Structure 12:1865-1875(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) IN COMPLEX WITH NADPH, SUBUNIT.

    Entry informationi

    Entry nameiACT3_STRCO
    AccessioniPrimary (citable) accession number: P16544
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: August 1, 1990
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3