Reviewed, UniProtKB/Swiss-Prot P16530 (PELX_ERWCA)

Names and origin

Protein names

Recommended name:
    Putative pectate lyase X
    EC=4.2.2.2

Gene names

Name:

PEL X

Organism

Erwinia carotovora

Taxonomic identifier

554 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in maceration and soft-rotting of plant tissue.
Catalytic activity	Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconic acid from pectin: step 2/5.
Induction	By pectin.
Sequence similarities	Belongs to the polysaccharide lyase 1 family.

Ontologies

Keywords
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Lyase
Gene Ontology (GO)
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW pectate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 238	216	Putative pectate lyase X		PRO_0000024855
Sites
	Metal binding	153	1	Calcium By similarity
	Metal binding	188	1	Calcium By similarity
	Metal binding	192	1	Calcium By similarity

Sequences

	Sequence		Length	Mass (Da)
	P16530-1 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: 46800EBA1CF41B64	FASTA	238	26,094
	10 20 30 40 50 60 MKYLLPTAAA GLLLLAAQPA MAANTGGYAT TDGGEVSGAV KKTARSMKEI VDIIEAAQVD 70 80 90 100 110 120 SKGKKVKGGA YPLIITYSGN EDSLIKAAEK NICGQWSKDA RGVQIKEFTK GTYYPGHQWL 130 140 150 160 170 180 IRQLRCLDCE TLLTLWYVIC ALAICQAARK HGDAIRIDNS PNVWIDHNEI FAKNFECKGT 190 200 210 220 230 PDNDTTFESA VDIKKGSTNV TVSVSVKEVG TLVNLSRLFF PFRIQRYRAF RLPVSCLP

References

[1]	"DNA structure of pectate lyase I gene cloned from Erwinia carotovora." Ito K., Kobayashi R., Nikaido N., Izaki K. Agric. Biol. Chem. 52:479-487(1988) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Er 18.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D00218 Genomic DNA. Translation: BAA00156.1.
3D structure databases
HSSP	HSSP built from PDB template 1AIR based on UniProtKB P11073.
ModBase	Search...
Protein family/group databases
CAZy	PL1. Polysaccharide Lyase Family 1.
Enzyme and pathway databases
BRENDA	4.2.2.2. 1498.
Family and domain databases
InterPro	IPR002022. Amb_allergen. IPR012334. Pectin_lyas_fold. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00544. Pec_lyase_C. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PELX_ERWCA

Accession

Primary (citable) accession number: P16530

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	April 14, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)