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Protein

DNA replication terminus site-binding protein

Gene

tus

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Trans-acting protein required for termination of DNA replication. Binds to DNA replication terminator sequences (terA to terF) to prevent the passage of replication forks. The termination efficiency will be affected by the affinity of this protein for the terminator sequence.

GO - Molecular functioni

  • sequence-specific DNA binding Source: EcoCyc

GO - Biological processi

  • DNA replication termination Source: EcoCyc
  • replication fork arrest involved in DNA replication termination Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

DNA replication

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG11038-MONOMER.
ECOL316407:JW1602-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication terminus site-binding proteinUniRule annotation
Short name:
Ter-binding proteinUniRule annotation
Gene namesi
Name:tusUniRule annotation
Synonyms:tau
Ordered Locus Names:b1610, JW1602
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11038. tus.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000494131 – 309DNA replication terminus site-binding proteinAdd BLAST309

Proteomic databases

PaxDbiP16525.
PRIDEiP16525.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi4260254. 85 interactors.
DIPiDIP-11056N.
IntActiP16525. 2 interactors.
MINTiMINT-584635.
STRINGi511145.b1610.

Structurei

Secondary structure

1309
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 28Combined sources23
Beta strandi33 – 40Combined sources8
Helixi46 – 48Combined sources3
Beta strandi49 – 51Combined sources3
Beta strandi54 – 56Combined sources3
Beta strandi59 – 62Combined sources4
Helixi63 – 73Combined sources11
Helixi79 – 81Combined sources3
Beta strandi84 – 86Combined sources3
Beta strandi88 – 90Combined sources3
Beta strandi96 – 102Combined sources7
Helixi104 – 129Combined sources26
Turni130 – 132Combined sources3
Turni136 – 138Combined sources3
Helixi139 – 146Combined sources8
Helixi152 – 156Combined sources5
Beta strandi161 – 164Combined sources4
Beta strandi167 – 173Combined sources7
Beta strandi176 – 181Combined sources6
Helixi183 – 193Combined sources11
Beta strandi194 – 197Combined sources4
Helixi206 – 220Combined sources15
Beta strandi228 – 233Combined sources6
Beta strandi237 – 244Combined sources8
Beta strandi247 – 254Combined sources8
Beta strandi259 – 264Combined sources6
Helixi265 – 267Combined sources3
Helixi282 – 284Combined sources3
Beta strandi296 – 300Combined sources5
Helixi301 – 303Combined sources3
Beta strandi305 – 308Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ECRX-ray2.70A1-309[»]
1SUTNMR-A223-244[»]
2EWJX-ray2.70A1-309[»]
2I05X-ray2.60A1-309[»]
2I06X-ray2.20A1-309[»]
4XR0X-ray2.80A1-309[»]
4XR1X-ray2.40A1-309[»]
4XR2X-ray2.35A1-309[»]
4XR3X-ray2.70A1-309[»]
ProteinModelPortaliP16525.
SMRiP16525.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP16525.

Family & Domainsi

Sequence similaritiesi

Belongs to the Tus family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105KR1. Bacteria.
ENOG410XSWR. LUCA.
HOGENOMiHOG000280323.
KOiK10748.
OMAiQVQYACP.

Family and domain databases

Gene3Di3.50.14.10. 2 hits.
HAMAPiMF_00483. Rep_term_Tus. 1 hit.
InterProiIPR008865. DNA_replication_term_site-bd.
[Graphical view]
PfamiPF05472. Ter. 1 hit.
[Graphical view]
SUPFAMiSSF56596. SSF56596. 1 hit.
TIGRFAMsiTIGR02648. rep_term_tus. 1 hit.

Sequencei

Sequence statusi: Complete.

P16525-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARYDLVDRL NTTFRQMEQE LAIFAAHLEQ HKLLVARVFS LPEVKKEDEH
60 70 80 90 100
NPLNRIEVKQ HLGNDAQSLA LRHFRHLFIQ QQSENRSSKA AVRLPGVLCY
110 120 130 140 150
QVDNLSQAAL VSHIQHINKL KTTFEHIVTV ESELPTAARF EWVHRHLPGL
160 170 180 190 200
ITLNAYRTLT VLHDPATLRF GWANKHIIKN LHRDEVLAQL EKSLKSPRSV
210 220 230 240 250
APWTREEWQR KLEREYQDIA ALPQNAKLKI KRPVKVQPIA RVWYKGDQKQ
260 270 280 290 300
VQHACPTPLI ALINRDNGAG VPDVGELLNY DADNVQHRYK PQAQPLRLII

PRLHLYVAD
Length:309
Mass (Da):35,783
Last modified:August 1, 1990 - v1
Checksum:iEC495E4D8E9DE887
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti85 – 102NRSSK…LCYQV → SQQQGHCPSAWLLCSGS in CAA27699 (PubMed:3541901).CuratedAdd BLAST18
Sequence conflicti121 – 122KT → EA in CAA27699 (PubMed:3541901).Curated2
Sequence conflicti134L → I in CAA27699 (PubMed:3541901).Curated1
Sequence conflicti150L → V in CAA27699 (PubMed:3541901).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90037 Genomic DNA. Translation: BAA14085.1.
U41101 Genomic DNA. Translation: AAA82083.1.
U00096 Genomic DNA. Translation: AAC74682.1.
AP009048 Genomic DNA. Translation: BAA15348.1.
X04065 Genomic DNA. Translation: CAA27699.1.
PIRiB32161. DNECTS.
RefSeqiNP_416127.1. NC_000913.3.
WP_000135181.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74682; AAC74682; b1610.
BAA15348; BAA15348; BAA15348.
GeneIDi945135.
KEGGiecj:JW1602.
eco:b1610.
PATRICi32118522. VBIEscCol129921_1681.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90037 Genomic DNA. Translation: BAA14085.1.
U41101 Genomic DNA. Translation: AAA82083.1.
U00096 Genomic DNA. Translation: AAC74682.1.
AP009048 Genomic DNA. Translation: BAA15348.1.
X04065 Genomic DNA. Translation: CAA27699.1.
PIRiB32161. DNECTS.
RefSeqiNP_416127.1. NC_000913.3.
WP_000135181.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ECRX-ray2.70A1-309[»]
1SUTNMR-A223-244[»]
2EWJX-ray2.70A1-309[»]
2I05X-ray2.60A1-309[»]
2I06X-ray2.20A1-309[»]
4XR0X-ray2.80A1-309[»]
4XR1X-ray2.40A1-309[»]
4XR2X-ray2.35A1-309[»]
4XR3X-ray2.70A1-309[»]
ProteinModelPortaliP16525.
SMRiP16525.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260254. 85 interactors.
DIPiDIP-11056N.
IntActiP16525. 2 interactors.
MINTiMINT-584635.
STRINGi511145.b1610.

Proteomic databases

PaxDbiP16525.
PRIDEiP16525.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74682; AAC74682; b1610.
BAA15348; BAA15348; BAA15348.
GeneIDi945135.
KEGGiecj:JW1602.
eco:b1610.
PATRICi32118522. VBIEscCol129921_1681.

Organism-specific databases

EchoBASEiEB1031.
EcoGeneiEG11038. tus.

Phylogenomic databases

eggNOGiENOG4105KR1. Bacteria.
ENOG410XSWR. LUCA.
HOGENOMiHOG000280323.
KOiK10748.
OMAiQVQYACP.

Enzyme and pathway databases

BioCyciEcoCyc:EG11038-MONOMER.
ECOL316407:JW1602-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP16525.
PROiP16525.

Family and domain databases

Gene3Di3.50.14.10. 2 hits.
HAMAPiMF_00483. Rep_term_Tus. 1 hit.
InterProiIPR008865. DNA_replication_term_site-bd.
[Graphical view]
PfamiPF05472. Ter. 1 hit.
[Graphical view]
SUPFAMiSSF56596. SSF56596. 1 hit.
TIGRFAMsiTIGR02648. rep_term_tus. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTUS_ECOLI
AccessioniPrimary (citable) accession number: P16525
Secondary accession number(s): Q59400
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: November 2, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.