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Protein

DNA replication terminus site-binding protein

Gene

tus

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Trans-acting protein required for termination of DNA replication. Binds to DNA replication terminator sequences (terA to terF) to prevent the passage of replication forks. The termination efficiency will be affected by the affinity of this protein for the terminator sequence.

GO - Molecular functioni

  • sequence-specific DNA binding Source: EcoCyc

GO - Biological processi

  • DNA replication termination Source: EcoCyc
  • replication fork arrest involved in DNA replication termination Source: EcoCyc

Keywordsi

Molecular functionDNA-binding
Biological processDNA replication

Enzyme and pathway databases

BioCyciEcoCyc:EG11038-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication terminus site-binding proteinUniRule annotation
Short name:
Ter-binding proteinUniRule annotation
Gene namesi
Name:tusUniRule annotation
Synonyms:tau
Ordered Locus Names:b1610, JW1602
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11038 tus

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000494131 – 309DNA replication terminus site-binding proteinAdd BLAST309

Proteomic databases

PaxDbiP16525
PRIDEiP16525

Interactioni

Subunit structurei

Monomer.

Binary interactionsi

WithEntry#Exp.IntActNotes
dnaBP0ACB03EBI-1124579,EBI-548978

Protein-protein interaction databases

BioGridi4260254, 85 interactors
849524, 1 interactor
DIPiDIP-11056N
IntActiP16525, 3 interactors
MINTiP16525
STRINGi316385.ECDH10B_1743

Structurei

Secondary structure

1309
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 28Combined sources23
Beta strandi33 – 40Combined sources8
Helixi46 – 48Combined sources3
Beta strandi49 – 51Combined sources3
Beta strandi54 – 56Combined sources3
Beta strandi59 – 62Combined sources4
Helixi63 – 73Combined sources11
Helixi79 – 81Combined sources3
Beta strandi84 – 86Combined sources3
Beta strandi88 – 90Combined sources3
Beta strandi96 – 102Combined sources7
Helixi104 – 129Combined sources26
Turni130 – 132Combined sources3
Turni136 – 138Combined sources3
Helixi139 – 146Combined sources8
Helixi152 – 156Combined sources5
Beta strandi161 – 164Combined sources4
Beta strandi167 – 173Combined sources7
Beta strandi176 – 181Combined sources6
Helixi183 – 193Combined sources11
Beta strandi194 – 197Combined sources4
Helixi206 – 220Combined sources15
Beta strandi228 – 233Combined sources6
Beta strandi237 – 244Combined sources8
Beta strandi247 – 254Combined sources8
Beta strandi259 – 264Combined sources6
Helixi265 – 267Combined sources3
Helixi282 – 284Combined sources3
Beta strandi296 – 300Combined sources5
Helixi301 – 303Combined sources3
Beta strandi305 – 308Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ECRX-ray2.70A1-309[»]
1SUTNMR-A223-244[»]
2EWJX-ray2.70A1-309[»]
2I05X-ray2.60A1-309[»]
2I06X-ray2.20A1-309[»]
4XR0X-ray2.80A1-309[»]
4XR1X-ray2.40A1-309[»]
4XR2X-ray2.35A1-309[»]
4XR3X-ray2.70A1-309[»]
ProteinModelPortaliP16525
SMRiP16525
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP16525

Family & Domainsi

Sequence similaritiesi

Belongs to the Tus family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105KR1 Bacteria
ENOG410XSWR LUCA
HOGENOMiHOG000280323
KOiK10748
OMAiQVQYACP

Family and domain databases

Gene3Di3.50.14.10, 2 hits
HAMAPiMF_00483 Rep_term_Tus, 1 hit
InterProiView protein in InterPro
IPR008865 DNA_replication_term_site-bd
IPR036381 Tus_dom1
IPR036384 Tus_sf
PfamiView protein in Pfam
PF05472 Ter, 1 hit
SUPFAMiSSF56596 SSF56596, 1 hit
TIGRFAMsiTIGR02648 rep_term_tus, 1 hit

Sequencei

Sequence statusi: Complete.

P16525-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARYDLVDRL NTTFRQMEQE LAIFAAHLEQ HKLLVARVFS LPEVKKEDEH
60 70 80 90 100
NPLNRIEVKQ HLGNDAQSLA LRHFRHLFIQ QQSENRSSKA AVRLPGVLCY
110 120 130 140 150
QVDNLSQAAL VSHIQHINKL KTTFEHIVTV ESELPTAARF EWVHRHLPGL
160 170 180 190 200
ITLNAYRTLT VLHDPATLRF GWANKHIIKN LHRDEVLAQL EKSLKSPRSV
210 220 230 240 250
APWTREEWQR KLEREYQDIA ALPQNAKLKI KRPVKVQPIA RVWYKGDQKQ
260 270 280 290 300
VQHACPTPLI ALINRDNGAG VPDVGELLNY DADNVQHRYK PQAQPLRLII

PRLHLYVAD
Length:309
Mass (Da):35,783
Last modified:August 1, 1990 - v1
Checksum:iEC495E4D8E9DE887
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti85 – 102NRSSK…LCYQV → SQQQGHCPSAWLLCSGS in CAA27699 (PubMed:3541901).CuratedAdd BLAST18
Sequence conflicti121 – 122KT → EA in CAA27699 (PubMed:3541901).Curated2
Sequence conflicti134L → I in CAA27699 (PubMed:3541901).Curated1
Sequence conflicti150L → V in CAA27699 (PubMed:3541901).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90037 Genomic DNA Translation: BAA14085.1
U41101 Genomic DNA Translation: AAA82083.1
U00096 Genomic DNA Translation: AAC74682.1
AP009048 Genomic DNA Translation: BAA15348.1
X04065 Genomic DNA Translation: CAA27699.1
PIRiB32161 DNECTS
RefSeqiNP_416127.1, NC_000913.3
WP_000135181.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74682; AAC74682; b1610
BAA15348; BAA15348; BAA15348
GeneIDi945135
KEGGiecj:JW1602
eco:b1610
PATRICifig|1411691.4.peg.652

Similar proteinsi

Entry informationi

Entry nameiTUS_ECOLI
AccessioniPrimary (citable) accession number: P16525
Secondary accession number(s): Q59400
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: March 28, 2018
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health