Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3

Gene

GNB3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

GO - Molecular functioni

  1. GTPase activity Source: ProtInc
  2. GTPase binding Source: UniProtKB
  3. signal transducer activity Source: UniProtKB-KW

GO - Biological processi

  1. cellular response to glucagon stimulus Source: Reactome
  2. energy reserve metabolic process Source: Reactome
  3. G-protein coupled receptor signaling pathway Source: ProtInc
  4. GTP catabolic process Source: GOC
  5. regulation of blood pressure Source: ProtInc
  6. small molecule metabolic process Source: Reactome
  7. synaptic transmission Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Transducer

Enzyme and pathway databases

ReactomeiREACT_15457. G-protein activation.
REACT_1665. Glucagon signaling in metabolic regulation.
REACT_172761. Ca2+ pathway.
REACT_18274. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
REACT_18283. G alpha (q) signalling events.
REACT_18339. Adrenaline,noradrenaline inhibits insulin secretion.
REACT_18377. Glucagon-type ligand receptors.
REACT_18407. G alpha (12/13) signalling events.
REACT_19140. ADP signalling through P2Y purinoceptor 1.
REACT_19145. G beta:gamma signalling through PLC beta.
REACT_19231. G alpha (i) signalling events.
REACT_19290. G beta:gamma signalling through PI3Kgamma.
REACT_19327. G alpha (s) signalling events.
REACT_19333. G alpha (z) signalling events.
REACT_20647. Thromboxane signalling through TP receptor.
REACT_20653. ADP signalling through P2Y purinoceptor 12.
REACT_21254. Presynaptic function of Kainate receptors.
REACT_21384. Thrombin signalling through proteinase activated receptors (PARs).
REACT_23946. Prostacyclin signalling through prostacyclin receptor.
REACT_24019. Synthesis, secretion, and inactivation of Glucagon-like Peptide-1 (GLP-1).
REACT_24023. Vasopressin regulates renal water homeostasis via Aquaporins.
REACT_25004. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.
REACT_75831. Activation of G protein gated Potassium channels.
SignaLinkiP16520.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3
Alternative name(s):
Transducin beta chain 3
Gene namesi
Name:GNB3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 12

Organism-specific databases

HGNCiHGNC:4400. GNB3.

Subcellular locationi

GO - Cellular componenti

  1. cell body Source: Ensembl
  2. extracellular vesicular exosome Source: UniProtKB
  3. neuron projection Source: Ensembl
  4. plasma membrane Source: Reactome
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA176.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 340340Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3PRO_0000127699Add
BLAST

Proteomic databases

MaxQBiP16520.
PaxDbiP16520.
PRIDEiP16520.

2D gel databases

OGPiP16520.

PTM databases

PhosphoSiteiP16520.

Expressioni

Gene expression databases

BgeeiP16520.
CleanExiHS_GNB3.
ExpressionAtlasiP16520. baseline and differential.
GenevestigatoriP16520.

Organism-specific databases

HPAiHPA040736.

Interactioni

Subunit structurei

G proteins are composed of 3 units, alpha, beta and gamma. Interacts with RASD2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CXCR5P323023EBI-2880663,EBI-2835269

Protein-protein interaction databases

BioGridi109046. 21 interactions.
DIPiDIP-601N.
IntActiP16520. 6 interactions.
MINTiMINT-2801250.
STRINGi9606.ENSP00000229264.

Structurei

3D structure databases

ProteinModelPortaliP16520.
SMRiP16520. Positions 3-340.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati53 – 8331WD 1Add
BLAST
Repeati95 – 12531WD 2Add
BLAST
Repeati141 – 17030WD 3Add
BLAST
Repeati182 – 21231WD 4Add
BLAST
Repeati224 – 25431WD 5Add
BLAST
Repeati268 – 29831WD 6Add
BLAST
Repeati310 – 34031WD 7Add
BLAST

Sequence similaritiesi

Belongs to the WD repeat G protein beta family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiCOG2319.
GeneTreeiENSGT00760000119239.
HOGENOMiHOG000176356.
HOVERGENiHBG000188.
InParanoidiP16520.
KOiK07825.
OMAiDSMKCER.
PhylomeDBiP16520.
TreeFamiTF106149.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 7 hits.
[Graphical view]
PIRSFiPIRSF002394. GN-bd_beta. 1 hit.
PRINTSiPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P16520-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGEMEQLRQE AEQLKKQIAD ARKACADVTL AELVSGLEVV GRVQMRTRRT
60 70 80 90 100
LRGHLAKIYA MHWATDSKLL VSASQDGKLI VWDSYTTNKV HAIPLRSSWV
110 120 130 140 150
MTCAYAPSGN FVACGGLDNM CSIYNLKSRE GNVKVSRELS AHTGYLSCCR
160 170 180 190 200
FLDDNNIVTS SGDTTCALWD IETGQQKTVF VGHTGDCMSL AVSPDFNLFI
210 220 230 240 250
SGACDASAKL WDVREGTCRQ TFTGHESDIN AICFFPNGEA ICTGSDDASC
260 270 280 290 300
RLFDLRADQE LICFSHESII CGITSVAFSL SGRLLFAGYD DFNCNVWDSM
310 320 330 340
KSERVGILSG HDNRVSCLGV TADGMAVATG SWDSFLKIWN
Length:340
Mass (Da):37,221
Last modified:August 1, 1990 - v1
Checksum:i896E706A61B8D74F
GO
Isoform 2 (identifier: P16520-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     236-278: Missing.

Note: No experimental confirmation available.

Show »
Length:297
Mass (Da):32,633
Checksum:i6A533D5BF825B273
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti40 – 401V → M.
Corresponds to variant rs45569331 [ dbSNP | Ensembl ].
VAR_049268
Natural varianti76 – 761D → N.
Corresponds to variant rs2234756 [ dbSNP | Ensembl ].
VAR_014756
Natural varianti81 – 811V → M.
Corresponds to variant rs45616032 [ dbSNP | Ensembl ].
VAR_049269
Natural varianti272 – 2721G → S.2 Publications
Corresponds to variant rs5442 [ dbSNP | Ensembl ].
VAR_014757
Natural varianti280 – 2801L → F.
Corresponds to variant rs28395776 [ dbSNP | Ensembl ].
VAR_029304
Natural varianti324 – 3241G → E.
Corresponds to variant rs28395775 [ dbSNP | Ensembl ].
VAR_029305
Natural varianti339 – 3391W → L.
Corresponds to variant rs5444 [ dbSNP | Ensembl ].
VAR_014758

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei236 – 27843Missing in isoform 2. 1 PublicationVSP_055233Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31328 mRNA. Translation: AAA52582.1.
U47924 Genomic DNA. Translation: AAB51313.1.
U47930 mRNA. Translation: AAC50468.1.
Y12050
, Y12051, Y12052, Y12053, Y12054, Y12055, Y12056, Y12057, Y12058 Genomic DNA. Translation: CAA72779.1.
AF501884 mRNA. Translation: AAM15920.1.
BT009800 mRNA. Translation: AAP88802.1.
AY631872 Genomic DNA. Translation: AAT38116.1.
BC000115 mRNA. Translation: AAH00115.1.
BC002454 mRNA. Translation: AAH02454.1.
BC015920 mRNA. Translation: AAH15920.1.
CCDSiCCDS8564.1. [P16520-1]
PIRiA35096. RGHUB3.
RefSeqiNP_001284500.1. NM_001297571.1.
NP_002066.1. NM_002075.3. [P16520-1]
UniGeneiHs.631657.

Genome annotation databases

EnsembliENST00000229264; ENSP00000229264; ENSG00000111664. [P16520-1]
GeneIDi2784.
KEGGihsa:2784.
UCSCiuc001qrc.3. human. [P16520-1]

Polymorphism databases

DMDMi121011.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31328 mRNA. Translation: AAA52582.1.
U47924 Genomic DNA. Translation: AAB51313.1.
U47930 mRNA. Translation: AAC50468.1.
Y12050
, Y12051, Y12052, Y12053, Y12054, Y12055, Y12056, Y12057, Y12058 Genomic DNA. Translation: CAA72779.1.
AF501884 mRNA. Translation: AAM15920.1.
BT009800 mRNA. Translation: AAP88802.1.
AY631872 Genomic DNA. Translation: AAT38116.1.
BC000115 mRNA. Translation: AAH00115.1.
BC002454 mRNA. Translation: AAH02454.1.
BC015920 mRNA. Translation: AAH15920.1.
CCDSiCCDS8564.1. [P16520-1]
PIRiA35096. RGHUB3.
RefSeqiNP_001284500.1. NM_001297571.1.
NP_002066.1. NM_002075.3. [P16520-1]
UniGeneiHs.631657.

3D structure databases

ProteinModelPortaliP16520.
SMRiP16520. Positions 3-340.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109046. 21 interactions.
DIPiDIP-601N.
IntActiP16520. 6 interactions.
MINTiMINT-2801250.
STRINGi9606.ENSP00000229264.

PTM databases

PhosphoSiteiP16520.

Polymorphism databases

DMDMi121011.

2D gel databases

OGPiP16520.

Proteomic databases

MaxQBiP16520.
PaxDbiP16520.
PRIDEiP16520.

Protocols and materials databases

DNASUi2784.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000229264; ENSP00000229264; ENSG00000111664. [P16520-1]
GeneIDi2784.
KEGGihsa:2784.
UCSCiuc001qrc.3. human. [P16520-1]

Organism-specific databases

CTDi2784.
GeneCardsiGC12P007133.
HGNCiHGNC:4400. GNB3.
HPAiHPA040736.
MIMi139130. gene.
neXtProtiNX_P16520.
PharmGKBiPA176.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG2319.
GeneTreeiENSGT00760000119239.
HOGENOMiHOG000176356.
HOVERGENiHBG000188.
InParanoidiP16520.
KOiK07825.
OMAiDSMKCER.
PhylomeDBiP16520.
TreeFamiTF106149.

Enzyme and pathway databases

ReactomeiREACT_15457. G-protein activation.
REACT_1665. Glucagon signaling in metabolic regulation.
REACT_172761. Ca2+ pathway.
REACT_18274. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
REACT_18283. G alpha (q) signalling events.
REACT_18339. Adrenaline,noradrenaline inhibits insulin secretion.
REACT_18377. Glucagon-type ligand receptors.
REACT_18407. G alpha (12/13) signalling events.
REACT_19140. ADP signalling through P2Y purinoceptor 1.
REACT_19145. G beta:gamma signalling through PLC beta.
REACT_19231. G alpha (i) signalling events.
REACT_19290. G beta:gamma signalling through PI3Kgamma.
REACT_19327. G alpha (s) signalling events.
REACT_19333. G alpha (z) signalling events.
REACT_20647. Thromboxane signalling through TP receptor.
REACT_20653. ADP signalling through P2Y purinoceptor 12.
REACT_21254. Presynaptic function of Kainate receptors.
REACT_21384. Thrombin signalling through proteinase activated receptors (PARs).
REACT_23946. Prostacyclin signalling through prostacyclin receptor.
REACT_24019. Synthesis, secretion, and inactivation of Glucagon-like Peptide-1 (GLP-1).
REACT_24023. Vasopressin regulates renal water homeostasis via Aquaporins.
REACT_25004. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.
REACT_75831. Activation of G protein gated Potassium channels.
SignaLinkiP16520.

Miscellaneous databases

ChiTaRSiGNB3. human.
GeneWikiiGNB3.
GenomeRNAii2784.
NextBioi10967.
PROiP16520.
SOURCEiSearch...

Gene expression databases

BgeeiP16520.
CleanExiHS_GNB3.
ExpressionAtlasiP16520. baseline and differential.
GenevestigatoriP16520.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 7 hits.
[Graphical view]
PIRSFiPIRSF002394. GN-bd_beta. 1 hit.
PRINTSiPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of beta 3 subunit, a third form of the G protein beta-subunit polypeptide."
    Levine M.A., Smallwood P.M., Moen P.T. Jr., Helman L.J., Ahn T.G.
    Proc. Natl. Acad. Sci. U.S.A. 87:2329-2333(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "A gene-rich cluster between the CD4 and triosephosphate isomerase genes at human chromosome 12p13."
    Ansari-Lari M.A., Muzny D.M., Lu J., Lu F., Lilley C.E., Spanos S., Malley T., Gibbs R.A.
    Genome Res. 6:314-326(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1).
  3. "Large-scale sequencing in human chromosome 12p13: experimental and computational gene structure determination."
    Ansari-Lari M.A., Shen Y., Muzny D.M., Lee W., Gibbs R.A.
    Genome Res. 7:268-280(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Brain.
  4. "G protein beta 3 gene: structure, promoter, and additional polymorphisms."
    Rosskopf D., Busch S., Manthey I., Siffert W.
    Hypertension 36:33-41(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
    Puhl H.L. III, Ikeda S.R., Aronstam R.S.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  6. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT SER-272.
  7. SeattleSNPs variation discovery resource
    Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT SER-272.
    Tissue: Eye.
  9. Bienvenut W.V., Heiserich L., Gottlieb E.
    Submitted (MAR-2008) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 69-78 AND 90-96, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Colon carcinoma.
  10. "The cationic region of Rhes mediates its interactions with specific Gbeta subunits."
    Hill C., Goddard A., Ladds G., Davey J.
    Cell. Physiol. Biochem. 23:1-8(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RASD2.

Entry informationi

Entry nameiGBB3_HUMAN
AccessioniPrimary (citable) accession number: P16520
Secondary accession number(s): Q96B71, Q9BQC0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: February 4, 2015
This is version 159 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.