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P16520 (GBB3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 153. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3
Alternative name(s):
Transducin beta chain 3
Gene names
Name:GNB3
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length340 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

Subunit structure

G proteins are composed of 3 units, alpha, beta and gamma. Interacts with RASD2. Ref.10

Sequence similarities

Belongs to the WD repeat G protein beta family.

Contains 7 WD repeats.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CXCR5P323023EBI-2880663,EBI-2835269

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P16520-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P16520-2)

The sequence of this isoform differs from the canonical sequence as follows:
     236-278: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 340340Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3
PRO_0000127699

Regions

Repeat53 – 8331WD 1
Repeat95 – 12531WD 2
Repeat141 – 17030WD 3
Repeat182 – 21231WD 4
Repeat224 – 25431WD 5
Repeat268 – 29831WD 6
Repeat310 – 34031WD 7

Natural variations

Alternative sequence236 – 27843Missing in isoform 2.
VSP_055233
Natural variant401V → M.
Corresponds to variant rs45569331 [ dbSNP | Ensembl ].
VAR_049268
Natural variant761D → N.
Corresponds to variant rs2234756 [ dbSNP | Ensembl ].
VAR_014756
Natural variant811V → M.
Corresponds to variant rs45616032 [ dbSNP | Ensembl ].
VAR_049269
Natural variant2721G → S. Ref.6 Ref.8
Corresponds to variant rs5442 [ dbSNP | Ensembl ].
VAR_014757
Natural variant2801L → F.
Corresponds to variant rs28395776 [ dbSNP | Ensembl ].
VAR_029304
Natural variant3241G → E.
Corresponds to variant rs28395775 [ dbSNP | Ensembl ].
VAR_029305
Natural variant3391W → L.
Corresponds to variant rs5444 [ dbSNP | Ensembl ].
VAR_014758

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified August 1, 1990. Version 1.
Checksum: 896E706A61B8D74F

FASTA34037,221
        10         20         30         40         50         60 
MGEMEQLRQE AEQLKKQIAD ARKACADVTL AELVSGLEVV GRVQMRTRRT LRGHLAKIYA 

        70         80         90        100        110        120 
MHWATDSKLL VSASQDGKLI VWDSYTTNKV HAIPLRSSWV MTCAYAPSGN FVACGGLDNM 

       130        140        150        160        170        180 
CSIYNLKSRE GNVKVSRELS AHTGYLSCCR FLDDNNIVTS SGDTTCALWD IETGQQKTVF 

       190        200        210        220        230        240 
VGHTGDCMSL AVSPDFNLFI SGACDASAKL WDVREGTCRQ TFTGHESDIN AICFFPNGEA 

       250        260        270        280        290        300 
ICTGSDDASC RLFDLRADQE LICFSHESII CGITSVAFSL SGRLLFAGYD DFNCNVWDSM 

       310        320        330        340 
KSERVGILSG HDNRVSCLGV TADGMAVATG SWDSFLKIWN 

« Hide

Isoform 2 [UniParc].

Checksum: 6A533D5BF825B273
Show »

FASTA29732,633

References

« Hide 'large scale' references
[1]"Molecular cloning of beta 3 subunit, a third form of the G protein beta-subunit polypeptide."
Levine M.A., Smallwood P.M., Moen P.T. Jr., Helman L.J., Ahn T.G.
Proc. Natl. Acad. Sci. U.S.A. 87:2329-2333(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"A gene-rich cluster between the CD4 and triosephosphate isomerase genes at human chromosome 12p13."
Ansari-Lari M.A., Muzny D.M., Lu J., Lu F., Lilley C.E., Spanos S., Malley T., Gibbs R.A.
Genome Res. 6:314-326(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1).
[3]"Large-scale sequencing in human chromosome 12p13: experimental and computational gene structure determination."
Ansari-Lari M.A., Shen Y., Muzny D.M., Lee W., Gibbs R.A.
Genome Res. 7:268-280(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Brain.
[4]"G protein beta 3 gene: structure, promoter, and additional polymorphisms."
Rosskopf D., Busch S., Manthey I., Siffert W.
Hypertension 36:33-41(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
Puhl H.L. III, Ikeda S.R., Aronstam R.S.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[6]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT SER-272.
[7]SeattleSNPs variation discovery resource
Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[8]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT SER-272.
Tissue: Eye.
[9]Bienvenut W.V., Heiserich L., Gottlieb E.
Submitted (MAR-2008) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 69-78 AND 90-96, IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: Colon carcinoma.
[10]"The cationic region of Rhes mediates its interactions with specific Gbeta subunits."
Hill C., Goddard A., Ladds G., Davey J.
Cell. Physiol. Biochem. 23:1-8(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RASD2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M31328 mRNA. Translation: AAA52582.1.
U47924 Genomic DNA. Translation: AAB51313.1.
U47930 mRNA. Translation: AAC50468.1.
Y12050 expand/collapse EMBL AC list , Y12051, Y12052, Y12053, Y12054, Y12055, Y12056, Y12057, Y12058 Genomic DNA. Translation: CAA72779.1.
AF501884 mRNA. Translation: AAM15920.1.
BT009800 mRNA. Translation: AAP88802.1.
AY631872 Genomic DNA. Translation: AAT38116.1.
BC000115 mRNA. Translation: AAH00115.1.
BC002454 mRNA. Translation: AAH02454.1.
BC015920 mRNA. Translation: AAH15920.1.
CCDSCCDS8564.1.
PIRRGHUB3. A35096.
RefSeqNP_002066.1. NM_002075.2.
UniGeneHs.631657.

3D structure databases

ProteinModelPortalP16520.
SMRP16520. Positions 3-340.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid109046. 15 interactions.
DIPDIP-601N.
IntActP16520. 6 interactions.
MINTMINT-2801250.
STRING9606.ENSP00000229264.

PTM databases

PhosphoSiteP16520.

Polymorphism databases

DMDM121011.

2D gel databases

OGPP16520.

Proteomic databases

MaxQBP16520.
PaxDbP16520.
PRIDEP16520.

Protocols and materials databases

DNASU2784.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000229264; ENSP00000229264; ENSG00000111664.
ENST00000596438; ENSP00000470228; ENSG00000268195.
GeneID2784.
KEGGhsa:2784.
UCSCuc001qrc.3. human.

Organism-specific databases

CTD2784.
GeneCardsGC12P007109.
HGNCHGNC:4400. GNB3.
HPAHPA040736.
MIM139130. gene.
neXtProtNX_P16520.
PharmGKBPA176.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG2319.
HOGENOMHOG000176356.
HOVERGENHBG000188.
InParanoidP16520.
KOK07825.
OMANMCSIYS.
PhylomeDBP16520.
TreeFamTF106149.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.
REACT_111217. Metabolism.
REACT_13685. Neuronal System.
REACT_15518. Transmembrane transport of small molecules.
REACT_604. Hemostasis.
SignaLinkP16520.

Gene expression databases

ArrayExpressP16520.
BgeeP16520.
CleanExHS_GNB3.
GenevestigatorP16520.

Family and domain databases

Gene3D2.130.10.10. 1 hit.
InterProIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamPF00400. WD40. 7 hits.
[Graphical view]
PIRSFPIRSF002394. GN-bd_beta. 1 hit.
PRINTSPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMSSF50978. SSF50978. 1 hit.
PROSITEPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSGNB3. human.
GeneWikiGNB3.
GenomeRNAi2784.
NextBio10967.
PROP16520.
SOURCESearch...

Entry information

Entry nameGBB3_HUMAN
AccessionPrimary (citable) accession number: P16520
Secondary accession number(s): Q96B71, Q9BQC0
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: July 9, 2014
This is version 153 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM