P16520 (GBB3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3

Alternative name(s):
Transducin beta chain 3

Gene names

Name:

GNB3

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.
Subunit structure	G proteins are composed of 3 units, alpha, beta and gamma. Interacts with RASD2. Ref.10
Sequence similarities	Belongs to the WD repeat G protein beta family. Contains 7 WD repeats.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Domain	Repeat WD repeat
Molecular function	Transducer
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	G-protein coupled receptor signaling pathway Traceable author statement. Source: ProtInc cellular response to glucagon stimulus Traceable author statement. Source: Reactome energy reserve metabolic process Traceable author statement. Source: Reactome regulation of blood pressure Traceable author statement. Source: ProtInc synaptic transmission Traceable author statement. Source: Reactome
Cellular component	plasma membrane Traceable author statement. Source: Reactome
Molecular function	GTPase activity Traceable author statement. Source: ProtInc GTPase binding Inferred from physical interaction Ref.10. Source: UniProtKB signal transducer activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 340	340	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3		PRO_0000127699
Regions
	Repeat	53 – 83	31	WD 1
	Repeat	95 – 125	31	WD 2
	Repeat	141 – 170	30	WD 3
	Repeat	182 – 212	31	WD 4
	Repeat	224 – 254	31	WD 5
	Repeat	268 – 298	31	WD 6
	Repeat	310 – 340	31	WD 7
Amino acid modifications
	Modified residue	74	1	Phosphoserine By similarity
Natural variations
	Natural variant	40	1	V → M. Corresponds to variant rs45569331 [ dbSNP | Ensembl ].		VAR_049268
	Natural variant	76	1	D → N. Corresponds to variant rs2234756 [ dbSNP | Ensembl ].		VAR_014756
	Natural variant	81	1	V → M. Corresponds to variant rs45616032 [ dbSNP | Ensembl ].		VAR_049269
	Natural variant	272	1	G → S. Ref.6 Ref.8 Corresponds to variant rs5442 [ dbSNP | Ensembl ].		VAR_014757
	Natural variant	280	1	L → F. Corresponds to variant rs28395776 [ dbSNP | Ensembl ].		VAR_029304
	Natural variant	324	1	G → E. Corresponds to variant rs28395775 [ dbSNP | Ensembl ].		VAR_029305
	Natural variant	339	1	W → L. Corresponds to variant rs5444 [ dbSNP | Ensembl ].		VAR_014758

Sequences

	Sequence		Length	Mass (Da)
	P16520 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: 896E706A61B8D74F	FASTA	340	37,221
	10 20 30 40 50 60 MGEMEQLRQE AEQLKKQIAD ARKACADVTL AELVSGLEVV GRVQMRTRRT LRGHLAKIYA 70 80 90 100 110 120 MHWATDSKLL VSASQDGKLI VWDSYTTNKV HAIPLRSSWV MTCAYAPSGN FVACGGLDNM 130 140 150 160 170 180 CSIYNLKSRE GNVKVSRELS AHTGYLSCCR FLDDNNIVTS SGDTTCALWD IETGQQKTVF 190 200 210 220 230 240 VGHTGDCMSL AVSPDFNLFI SGACDASAKL WDVREGTCRQ TFTGHESDIN AICFFPNGEA 250 260 270 280 290 300 ICTGSDDASC RLFDLRADQE LICFSHESII CGITSVAFSL SGRLLFAGYD DFNCNVWDSM 310 320 330 340 KSERVGILSG HDNRVSCLGV TADGMAVATG SWDSFLKIWN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of beta 3 subunit, a third form of the G protein beta-subunit polypeptide." Levine M.A., Smallwood P.M., Moen P.T. Jr., Helman L.J., Ahn T.G. Proc. Natl. Acad. Sci. U.S.A. 87:2329-2333(1990) [PubMed: 2107550] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"A gene-rich cluster between the CD4 and triosephosphate isomerase genes at human chromosome 12p13." Ansari-Lari M.A., Muzny D.M., Lu J., Lu F., Lilley C.E., Spanos S., Malley T., Gibbs R.A. Genome Res. 6:314-326(1996) [PubMed: 8723724] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[3]	"Large-scale sequencing in human chromosome 12p13: experimental and computational gene structure determination." Ansari-Lari M.A., Shen Y., Muzny D.M., Lee W., Gibbs R.A. Genome Res. 7:268-280(1997) [PubMed: 9074930] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Brain.
[4]	"G protein beta 3 gene: structure, promoter, and additional polymorphisms." Rosskopf D., Busch S., Manthey I., Siffert W. Hypertension 36:33-41(2000) [PubMed: 10904009] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[6]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT SER-272.
[7]	SeattleSNPs variation discovery resource Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT SER-272.
[9]	Bienvenut W.V., Heiserich L., Gottlieb E. Submitted (MAR-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 69-78 AND 90-96, MASS SPECTROMETRY. Tissue: Colon carcinoma.
[10]	"The cationic region of Rhes mediates its interactions with specific Gbeta subunits." Hill C., Goddard A., Ladds G., Davey J. Cell. Physiol. Biochem. 23:1-8(2009) [PubMed: 19255495] [Abstract] Cited for: INTERACTION WITH RASD2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M31328 mRNA. Translation: AAA52582.1. U47924 Genomic DNA. Translation: AAB51313.1. U47930 mRNA. Translation: AAC50468.1. Y12050 , Y12051, Y12052, Y12053, Y12054, Y12055, Y12056, Y12057, Y12058 Genomic DNA. Translation: CAA72779.1. AF501884 mRNA. Translation: AAM15920.1. BT009800 mRNA. Translation: AAP88802.1. AY631872 Genomic DNA. Translation: AAT38116.1. BC000115 mRNA. Translation: AAH00115.1. BC002454 mRNA. Translation: AAH02454.1.
IPI	IPI00029143.
PIR	RGHUB3. A35096.
RefSeq	NP_002066.1. NM_002075.2.
UniGene	Hs.631657.
3D structure databases
ProteinModelPortal	P16520.
SMR	P16520. Positions 3-340.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-601N.
IntAct	P16520. 1 interaction.
STRING	P16520.
PTM databases
PhosphoSite	P16520.
Polymorphism databases
DMDM	121011.
2D gel databases
OGP	P16520.
Proteomic databases
PRIDE	P16520.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000229264; ENSP00000229264; ENSG00000111664.
GeneID	2784.
KEGG	hsa:2784.
UCSC	uc001qrd.1. human.
Organism-specific databases
CTD	2784.
GeneCards	GC12P007001.
H-InvDB	HIX0201854.
HGNC	HGNC:4400. GNB3.
HPA	HPA005645.
MIM	139130. gene.
neXtProt	NX_P16520.
PharmGKB	PA176.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG16245.
HOGENOM	HBG396231.
HOVERGEN	HBG000188.
InParanoid	P16520.
OMA	NMCSIYS.
OrthoDB	EOG4J118C.
PhylomeDB	P16520.
Enzyme and pathway databases
Pathway_Interaction_DB	cone_pathway. Visual signal transduction: Cones.
Reactome	REACT_111102. Signal Transduction. REACT_111217. Metabolism. REACT_13685. Neuronal System. REACT_15518. Transmembrane transport of small molecules. REACT_604. Hemostasis.
Gene expression databases
ArrayExpress	P16520.
Bgee	P16520.
CleanEx	HS_GNB3.
Genevestigator	P16520.
GermOnline	ENSG00000111664. Homo sapiens.
Family and domain databases
InterPro	IPR020472. G-protein_beta_WD-40_rep. IPR001632. Gprotein_B. IPR016346. Guanine_nucleotide-bd_bsu. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR011046. WD40_repeat-like_dom. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
KO	K07825.
Pfam	PF00400. WD40. 7 hits. [Graphical view]
PIRSF	PIRSF002394. GN-bd_beta. 1 hit.
PRINTS	PR00319. GPROTEINB. PR00320. GPROTEINBRPT.
SMART	SM00320. WD40. 7 hits. [Graphical view]
SUPFAM	SSF50978. WD40_like. 1 hit.
PROSITE	PS00678. WD_REPEATS_1. 3 hits. PS50082. WD_REPEATS_2. 5 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	10967.
SOURCE	Search...

Entry information

Entry name

GBB3_HUMAN

Accession

Primary (citable) accession number: P16520
Secondary accession number(s): Q9BQC0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	January 25, 2012
This is version 127 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.