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P16520

- GBB3_HUMAN

UniProt

P16520 - GBB3_HUMAN

Protein

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3

Gene

GNB3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 155 (01 Oct 2014)
      Sequence version 1 (01 Aug 1990)
      Previous versions | rss
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    Functioni

    Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

    GO - Molecular functioni

    1. GTPase activity Source: ProtInc
    2. GTPase binding Source: UniProtKB
    3. protein binding Source: IntAct
    4. signal transducer activity Source: UniProtKB-KW

    GO - Biological processi

    1. cellular response to glucagon stimulus Source: Reactome
    2. energy reserve metabolic process Source: Reactome
    3. G-protein coupled receptor signaling pathway Source: ProtInc
    4. GTP catabolic process Source: GOC
    5. regulation of blood pressure Source: ProtInc
    6. small molecule metabolic process Source: Reactome
    7. synaptic transmission Source: Reactome

    Keywords - Molecular functioni

    Transducer

    Enzyme and pathway databases

    ReactomeiREACT_15457. G-protein activation.
    REACT_1665. Glucagon signaling in metabolic regulation.
    REACT_172761. Ca2+ pathway.
    REACT_18274. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
    REACT_18283. G alpha (q) signalling events.
    REACT_18339. Adrenaline,noradrenaline inhibits insulin secretion.
    REACT_18377. Glucagon-type ligand receptors.
    REACT_18407. G alpha (12/13) signalling events.
    REACT_19140. ADP signalling through P2Y purinoceptor 1.
    REACT_19145. G beta:gamma signalling through PLC beta.
    REACT_19231. G alpha (i) signalling events.
    REACT_19290. G beta:gamma signalling through PI3Kgamma.
    REACT_19327. G alpha (s) signalling events.
    REACT_19333. G alpha (z) signalling events.
    REACT_20647. Thromboxane signalling through TP receptor.
    REACT_20653. ADP signalling through P2Y purinoceptor 12.
    REACT_21254. Presynaptic function of Kainate receptors.
    REACT_21384. Thrombin signalling through proteinase activated receptors (PARs).
    REACT_23946. Prostacyclin signalling through prostacyclin receptor.
    REACT_24019. Synthesis, secretion, and inactivation of Glucagon-like Peptide-1 (GLP-1).
    REACT_24023. Regulation of water balance by renal Aquaporins.
    REACT_25004. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.
    REACT_75831. Activation of G protein gated Potassium channels.
    SignaLinkiP16520.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3
    Alternative name(s):
    Transducin beta chain 3
    Gene namesi
    Name:GNB3
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 12

    Organism-specific databases

    HGNCiHGNC:4400. GNB3.

    Subcellular locationi

    GO - Cellular componenti

    1. cell body Source: Ensembl
    2. neuron projection Source: Ensembl
    3. plasma membrane Source: Reactome

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA176.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 340340Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3PRO_0000127699Add
    BLAST

    Proteomic databases

    MaxQBiP16520.
    PaxDbiP16520.
    PRIDEiP16520.

    2D gel databases

    OGPiP16520.

    PTM databases

    PhosphoSiteiP16520.

    Expressioni

    Gene expression databases

    ArrayExpressiP16520.
    BgeeiP16520.
    CleanExiHS_GNB3.
    GenevestigatoriP16520.

    Organism-specific databases

    HPAiHPA040736.

    Interactioni

    Subunit structurei

    G proteins are composed of 3 units, alpha, beta and gamma. Interacts with RASD2.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CXCR5P323023EBI-2880663,EBI-2835269

    Protein-protein interaction databases

    BioGridi109046. 15 interactions.
    DIPiDIP-601N.
    IntActiP16520. 6 interactions.
    MINTiMINT-2801250.
    STRINGi9606.ENSP00000229264.

    Structurei

    3D structure databases

    ProteinModelPortaliP16520.
    SMRiP16520. Positions 3-340.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati53 – 8331WD 1Add
    BLAST
    Repeati95 – 12531WD 2Add
    BLAST
    Repeati141 – 17030WD 3Add
    BLAST
    Repeati182 – 21231WD 4Add
    BLAST
    Repeati224 – 25431WD 5Add
    BLAST
    Repeati268 – 29831WD 6Add
    BLAST
    Repeati310 – 34031WD 7Add
    BLAST

    Sequence similaritiesi

    Belongs to the WD repeat G protein beta family.Curated
    Contains 7 WD repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, WD repeat

    Phylogenomic databases

    eggNOGiCOG2319.
    HOGENOMiHOG000176356.
    HOVERGENiHBG000188.
    InParanoidiP16520.
    KOiK07825.
    OMAiNMCSIYS.
    PhylomeDBiP16520.
    TreeFamiTF106149.

    Family and domain databases

    Gene3Di2.130.10.10. 1 hit.
    InterProiIPR020472. G-protein_beta_WD-40_rep.
    IPR001632. Gprotein_B.
    IPR016346. Guanine_nucleotide-bd_bsu.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF00400. WD40. 7 hits.
    [Graphical view]
    PIRSFiPIRSF002394. GN-bd_beta. 1 hit.
    PRINTSiPR00319. GPROTEINB.
    PR00320. GPROTEINBRPT.
    SMARTiSM00320. WD40. 7 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 1 hit.
    PROSITEiPS00678. WD_REPEATS_1. 3 hits.
    PS50082. WD_REPEATS_2. 5 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P16520-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGEMEQLRQE AEQLKKQIAD ARKACADVTL AELVSGLEVV GRVQMRTRRT    50
    LRGHLAKIYA MHWATDSKLL VSASQDGKLI VWDSYTTNKV HAIPLRSSWV 100
    MTCAYAPSGN FVACGGLDNM CSIYNLKSRE GNVKVSRELS AHTGYLSCCR 150
    FLDDNNIVTS SGDTTCALWD IETGQQKTVF VGHTGDCMSL AVSPDFNLFI 200
    SGACDASAKL WDVREGTCRQ TFTGHESDIN AICFFPNGEA ICTGSDDASC 250
    RLFDLRADQE LICFSHESII CGITSVAFSL SGRLLFAGYD DFNCNVWDSM 300
    KSERVGILSG HDNRVSCLGV TADGMAVATG SWDSFLKIWN 340
    Length:340
    Mass (Da):37,221
    Last modified:August 1, 1990 - v1
    Checksum:i896E706A61B8D74F
    GO
    Isoform 2 (identifier: P16520-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         236-278: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:297
    Mass (Da):32,633
    Checksum:i6A533D5BF825B273
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti40 – 401V → M.
    Corresponds to variant rs45569331 [ dbSNP | Ensembl ].
    VAR_049268
    Natural varianti76 – 761D → N.
    Corresponds to variant rs2234756 [ dbSNP | Ensembl ].
    VAR_014756
    Natural varianti81 – 811V → M.
    Corresponds to variant rs45616032 [ dbSNP | Ensembl ].
    VAR_049269
    Natural varianti272 – 2721G → S.2 Publications
    Corresponds to variant rs5442 [ dbSNP | Ensembl ].
    VAR_014757
    Natural varianti280 – 2801L → F.
    Corresponds to variant rs28395776 [ dbSNP | Ensembl ].
    VAR_029304
    Natural varianti324 – 3241G → E.
    Corresponds to variant rs28395775 [ dbSNP | Ensembl ].
    VAR_029305
    Natural varianti339 – 3391W → L.
    Corresponds to variant rs5444 [ dbSNP | Ensembl ].
    VAR_014758

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei236 – 27843Missing in isoform 2. 1 PublicationVSP_055233Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M31328 mRNA. Translation: AAA52582.1.
    U47924 Genomic DNA. Translation: AAB51313.1.
    U47930 mRNA. Translation: AAC50468.1.
    Y12050
    , Y12051, Y12052, Y12053, Y12054, Y12055, Y12056, Y12057, Y12058 Genomic DNA. Translation: CAA72779.1.
    AF501884 mRNA. Translation: AAM15920.1.
    BT009800 mRNA. Translation: AAP88802.1.
    AY631872 Genomic DNA. Translation: AAT38116.1.
    BC000115 mRNA. Translation: AAH00115.1.
    BC002454 mRNA. Translation: AAH02454.1.
    BC015920 mRNA. Translation: AAH15920.1.
    CCDSiCCDS8564.1. [P16520-1]
    PIRiA35096. RGHUB3.
    RefSeqiNP_002066.1. NM_002075.2.
    UniGeneiHs.631657.

    Genome annotation databases

    EnsembliENST00000229264; ENSP00000229264; ENSG00000111664. [P16520-1]
    GeneIDi2784.
    KEGGihsa:2784.
    UCSCiuc001qrc.3. human. [P16520-1]

    Polymorphism databases

    DMDMi121011.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M31328 mRNA. Translation: AAA52582.1 .
    U47924 Genomic DNA. Translation: AAB51313.1 .
    U47930 mRNA. Translation: AAC50468.1 .
    Y12050
    , Y12051 , Y12052 , Y12053 , Y12054 , Y12055 , Y12056 , Y12057 , Y12058 Genomic DNA. Translation: CAA72779.1 .
    AF501884 mRNA. Translation: AAM15920.1 .
    BT009800 mRNA. Translation: AAP88802.1 .
    AY631872 Genomic DNA. Translation: AAT38116.1 .
    BC000115 mRNA. Translation: AAH00115.1 .
    BC002454 mRNA. Translation: AAH02454.1 .
    BC015920 mRNA. Translation: AAH15920.1 .
    CCDSi CCDS8564.1. [P16520-1 ]
    PIRi A35096. RGHUB3.
    RefSeqi NP_002066.1. NM_002075.2.
    UniGenei Hs.631657.

    3D structure databases

    ProteinModelPortali P16520.
    SMRi P16520. Positions 3-340.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 109046. 15 interactions.
    DIPi DIP-601N.
    IntActi P16520. 6 interactions.
    MINTi MINT-2801250.
    STRINGi 9606.ENSP00000229264.

    PTM databases

    PhosphoSitei P16520.

    Polymorphism databases

    DMDMi 121011.

    2D gel databases

    OGPi P16520.

    Proteomic databases

    MaxQBi P16520.
    PaxDbi P16520.
    PRIDEi P16520.

    Protocols and materials databases

    DNASUi 2784.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000229264 ; ENSP00000229264 ; ENSG00000111664 . [P16520-1 ]
    GeneIDi 2784.
    KEGGi hsa:2784.
    UCSCi uc001qrc.3. human. [P16520-1 ]

    Organism-specific databases

    CTDi 2784.
    GeneCardsi GC12P007109.
    HGNCi HGNC:4400. GNB3.
    HPAi HPA040736.
    MIMi 139130. gene.
    neXtProti NX_P16520.
    PharmGKBi PA176.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG2319.
    HOGENOMi HOG000176356.
    HOVERGENi HBG000188.
    InParanoidi P16520.
    KOi K07825.
    OMAi NMCSIYS.
    PhylomeDBi P16520.
    TreeFami TF106149.

    Enzyme and pathway databases

    Reactomei REACT_15457. G-protein activation.
    REACT_1665. Glucagon signaling in metabolic regulation.
    REACT_172761. Ca2+ pathway.
    REACT_18274. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
    REACT_18283. G alpha (q) signalling events.
    REACT_18339. Adrenaline,noradrenaline inhibits insulin secretion.
    REACT_18377. Glucagon-type ligand receptors.
    REACT_18407. G alpha (12/13) signalling events.
    REACT_19140. ADP signalling through P2Y purinoceptor 1.
    REACT_19145. G beta:gamma signalling through PLC beta.
    REACT_19231. G alpha (i) signalling events.
    REACT_19290. G beta:gamma signalling through PI3Kgamma.
    REACT_19327. G alpha (s) signalling events.
    REACT_19333. G alpha (z) signalling events.
    REACT_20647. Thromboxane signalling through TP receptor.
    REACT_20653. ADP signalling through P2Y purinoceptor 12.
    REACT_21254. Presynaptic function of Kainate receptors.
    REACT_21384. Thrombin signalling through proteinase activated receptors (PARs).
    REACT_23946. Prostacyclin signalling through prostacyclin receptor.
    REACT_24019. Synthesis, secretion, and inactivation of Glucagon-like Peptide-1 (GLP-1).
    REACT_24023. Regulation of water balance by renal Aquaporins.
    REACT_25004. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.
    REACT_75831. Activation of G protein gated Potassium channels.
    SignaLinki P16520.

    Miscellaneous databases

    ChiTaRSi GNB3. human.
    GeneWikii GNB3.
    GenomeRNAii 2784.
    NextBioi 10967.
    PROi P16520.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P16520.
    Bgeei P16520.
    CleanExi HS_GNB3.
    Genevestigatori P16520.

    Family and domain databases

    Gene3Di 2.130.10.10. 1 hit.
    InterProi IPR020472. G-protein_beta_WD-40_rep.
    IPR001632. Gprotein_B.
    IPR016346. Guanine_nucleotide-bd_bsu.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    Pfami PF00400. WD40. 7 hits.
    [Graphical view ]
    PIRSFi PIRSF002394. GN-bd_beta. 1 hit.
    PRINTSi PR00319. GPROTEINB.
    PR00320. GPROTEINBRPT.
    SMARTi SM00320. WD40. 7 hits.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 1 hit.
    PROSITEi PS00678. WD_REPEATS_1. 3 hits.
    PS50082. WD_REPEATS_2. 5 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of beta 3 subunit, a third form of the G protein beta-subunit polypeptide."
      Levine M.A., Smallwood P.M., Moen P.T. Jr., Helman L.J., Ahn T.G.
      Proc. Natl. Acad. Sci. U.S.A. 87:2329-2333(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "A gene-rich cluster between the CD4 and triosephosphate isomerase genes at human chromosome 12p13."
      Ansari-Lari M.A., Muzny D.M., Lu J., Lu F., Lilley C.E., Spanos S., Malley T., Gibbs R.A.
      Genome Res. 6:314-326(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1).
    3. "Large-scale sequencing in human chromosome 12p13: experimental and computational gene structure determination."
      Ansari-Lari M.A., Shen Y., Muzny D.M., Lee W., Gibbs R.A.
      Genome Res. 7:268-280(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Tissue: Brain.
    4. "G protein beta 3 gene: structure, promoter, and additional polymorphisms."
      Rosskopf D., Busch S., Manthey I., Siffert W.
      Hypertension 36:33-41(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    5. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
      Puhl H.L. III, Ikeda S.R., Aronstam R.S.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain.
    6. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT SER-272.
    7. SeattleSNPs variation discovery resource
      Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT SER-272.
      Tissue: Eye.
    9. Bienvenut W.V., Heiserich L., Gottlieb E.
      Submitted (MAR-2008) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 69-78 AND 90-96, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Colon carcinoma.
    10. "The cationic region of Rhes mediates its interactions with specific Gbeta subunits."
      Hill C., Goddard A., Ladds G., Davey J.
      Cell. Physiol. Biochem. 23:1-8(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RASD2.

    Entry informationi

    Entry nameiGBB3_HUMAN
    AccessioniPrimary (citable) accession number: P16520
    Secondary accession number(s): Q96B71, Q9BQC0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: August 1, 1990
    Last modified: October 1, 2014
    This is version 155 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 12
      Human chromosome 12: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3