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P16512 (RDRP_I77AD) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
RNA-directed RNA polymerase catalytic subunit

EC=2.7.7.48
Alternative name(s):
Polymerase basic protein 1
Short name=PB1
RNA-directed RNA polymerase subunit P1
Gene names
Name:PB1
OrganismInfluenza A virus (strain A/Swine/Tennessee/26/1977 H1N1)
Taxonomic identifier384479 [NCBI]
Taxonomic lineageVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length757 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

RNA-dependent RNA polymerase which is responsible for replication and transcription of virus segments. Binds the promoter sequence of the encapsidated viral RNA. Displays an endonuclease activity involved in cap-stealing. Cleaves cellular pre-mRNA to generate primers for viral transcription.

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Subunit structure

Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA.

Subcellular location

Host nucleus Potential.

Sequence similarities

Belongs to the influenza viruses polymerase PB1 family.

Contains 1 RdRp catalytic domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 757757RNA-directed RNA polymerase catalytic subunit
PRO_0000078771

Regions

Domain286 – 483198RdRp catalytic
Region249 – 2568Promoter-binding site By similarity
Motif187 – 1959Nuclear localization signal By similarity
Motif203 – 21614Nuclear localization signal By similarity

Sequences

Sequence LengthMass (Da)Tools
P16512 [UniParc].

Last modified August 1, 1990. Version 1.
Checksum: C8DB0DA2959C67BD

FASTA75786,344
        10         20         30         40         50         60 
MDVNPTLLFL KVPAQNAIST TFPYTGDPPY SHGTGTGYTM DTVNRTHQYS ERGKWTTNTE 

        70         80         90        100        110        120 
TGAPQLNPID GPLPEDNEPT GYAQTDCVLE AMAFLEKSHP GIFENSCLET MEVVQQTRVD 

       130        140        150        160        170        180 
KLTQGRQTFD WTLNRNQPAA TALANTIEVF RLNGLTASES GRLIDFLKDV MESMDKEEME 

       190        200        210        220        230        240 
IVTHFQRKRR VRDNMTKKMV TQRTIGKKKQ KLNKRSYIIR ALTLNTMTKD AERGKLKRRA 

       250        260        270        280        290        300 
IATPGMQIRG FVYFVETLAR SICEKLEQSG LPVGGNEKKA KLANVVRKMM TNSQDTELSF 

       310        320        330        340        350        360 
TITGDNTKWN ENQNPRMFLA MITYITRNQP EWFRNVLSIA PIMFSNKMAR LGKGYMFESK 

       370        380        390        400        410        420 
NMKLRTQIPA EMLSGIDLRY FNDSTRKKIE KIRPLLIDGA ASLSPGMMMG MFNMLSTVLG 

       430        440        450        460        470        480 
VSILNLGQKE YTKTAYWWDG LQSSDDFALI VNALNHEGIQ AGVDRFYRTC KLLGINMSKK 

       490        500        510        520        530        540 
KSYINRTGTF EFTSFFYRYG FVANFSMELP SFGVSGINES ADMSIGVTVI KNNMINNDLG 

       550        560        570        580        590        600 
PATAQMALQL FIKDYRYTYR CHRGDTQIQT RRSFEIKKLW DQTHSKAGLL VSDGGPNLYN 

       610        620        630        640        650        660 
IRNLHIPEVC LKWELMDKDY QGRLCNPLNP FVSHKEIESV NNAVVMPSHG PAKTMEYDAV 

       670        680        690        700        710        720 
ATTHSWVPKR NRSILNTSQR GILEDEQMYQ KCCNLFEKFF PSSSYRRPVG ISSMVEAMVS 

       730        740        750 
RARIDARIDF ESGRIKKEDF AEIMKICSTI EDLRRQK 

« Hide

References

[1]"Avian-to-human transmission of the PB1 gene of influenza A viruses in the 1957 and 1968 pandemics."
Kawaoka Y., Krauss S., Webster R.G.
J. Virol. 63:4603-4608(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M25931 Genomic RNA. Translation: AAA43640.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR007099. RNA-dir_pol_NSvirus.
IPR001407. RNA_pol_PB1_influenza.
[Graphical view]
PfamPF00602. Flu_PB1. 1 hit.
[Graphical view]
PIRSFPIRSF000827. RdRPol_OMV. 1 hit.
PROSITEPS50525. RDRP_SSRNA_NEG_SEG. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRDRP_I77AD
AccessionPrimary (citable) accession number: P16512
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: April 16, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families