P16502 (RDRP_I56A0) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-directed RNA polymerase catalytic subunit
EC=2.7.7.48

Alternative name(s):
Polymerase basic protein 1
Short name=PB1
RNA-directed RNA polymerase subunit P1

Gene names

Name:

PB1

Organism

Influenza A virus (strain A/Beijing/11/1956 H1N1)

Taxonomic identifier

384500 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA negative-strand viruses › Orthomyxoviridae › Influenzavirus A › Influenza A virus › H1N1 subtype

Virus host

Aves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length	757 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	RNA-dependent RNA polymerase which is responsible for replication and transcription of virus segments. Binds the promoter sequence of the encapsidated viral RNA. Displays an endonuclease activity involved in cap-stealing. Cleaves cellular pre-mRNA to generate primers for viral transcription.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA.
Subcellular location	Host nucleus Potential.
Sequence similarities	Belongs to the influenza viruses polymerase PB1 family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	Host gene expression shutoff by virus Host transcription shutoff by virus Host-virus interaction Inhibition of host RNA polymerase II by virus Viral RNA replication
Cellular component	Host nucleus
Ligand	Nucleotide-binding
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Gene Ontology (GO)
Biological_process	suppression by virus of host RNA polymerase II activity Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro viral genome replication Inferred from electronic annotation. Source: InterPro
Cellular_component	host cell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 757	757	RNA-directed RNA polymerase catalytic subunit		PRO_0000078745
Regions
	Domain	286 – 483	198	RdRp catalytic
	Region	249 – 256	8	Promoter-binding site By similarity
	Motif	187 – 195	9	Nuclear localization signal By similarity
	Motif	203 – 216	14	Nuclear localization signal By similarity

Sequences

	Sequence		Length	Mass (Da)
	P16502 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: 99352A1EF01109DC	FASTA	757	86,535
	10 20 30 40 50 60 MDVNPTLLFL KVPAQNAIST TFPYTGDPPY SHGTGTGYTM DTVNRTHQYS ERGRWTKNTE 70 80 90 100 110 120 TGAPQLNPID GPLPEDNEPS GYAQTDCVLE AMAFLEESHP GIFENSCIET MEVVQQTRVD 130 140 150 160 170 180 KLTQGRQTYD WTLNRNQPAA TALANTIEVF RSNGLMANES GRLIDFLKDV MESMDKEEIE 190 200 210 220 230 240 ITTHFQRKRR VRDNVTKKMV TQRTIGKKKQ RLNKRSYLIR ALTLNTMTKD AERGKLKRRA 250 260 270 280 290 300 IATPGMQIRG FVYFVETLAR SICEKLEQSG LPVGGNEKKA KLANVVRKMM TNSQDTEISF 310 320 330 340 350 360 TITGDNTKWN ENQNPRMFLA VITYITRNQP EWFRNILSIA PIMFSNKMAR LGKGYMFESK 370 380 390 400 410 420 SMKLRTQISA EMLANIDLKY FNDSTRKKIE KIRPLLIDGT ASLSPGMMMG MFNMLSTVLG 430 440 450 460 470 480 VSILNLGQKR YTKTTYWWDG LQSSDDFALI VNAPNHAGIQ AGVDRFYRTC KLLGINMSKK 490 500 510 520 530 540 KSYINRTGTF EFTSFFYRYG FVANFSMELP SFGVSGINES ADMSIGVTVI KNNMINNDLG 550 560 570 580 590 600 PATAQMALQL FIKDYRYTYR CHRGDTQIQT RRSFEIKKLW EQTRSKAGLL VSDGGPNLYN 610 620 630 640 650 660 IRNLHTPEVC LKWELMDEDY QGRLCNPLNP FVSHKEIESV NNAVMMPAHG PAKNMEYDAV 670 680 690 700 710 720 ATTHSWVPKR NRSILNTSQR GILEDEQMYQ RCCNLFERFF PSSSYRRPVG ISSMVEAMVS 730 740 750 RARIDARIDF ESGRIKKEDF TEIMKICSTI EELRRQK

References

[1]	"Avian-to-human transmission of the PB1 gene of influenza A viruses in the 1957 and 1968 pandemics." Kawaoka Y., Krauss S., Webster R.G. J. Virol. 63:4603-4608(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M25932 Genomic RNA. Translation: AAA43641.1.
3D structure databases
ProteinModelPortal	P16502.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR007099. RNA-dir_pol_NSvirus. IPR001407. RNA_pol_PB1_influenza. [Graphical view]
Pfam	PF00602. Flu_PB1. 1 hit. [Graphical view]
PIRSF	PIRSF000827. RdRPol_OMV. 1 hit.
PROSITE	PS50525. RDRP_SSRNA_NEG_SEG. 1 hit. [Graphical view]
ProtoNet	Search...
Other
DrugBank	DB00811. Ribavirin.

Entry information

Entry name

RDRP_I56A0

Accession

Primary (citable) accession number: P16502

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	April 3, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program