P16501 (IGF1A_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Insulin-like growth factor I-A
Short name=IGF-I'
Short name=IGF-IA
Short name=xIGF-1

Alternative name(s):
Somatomedin

Gene names

Name:

igf1-a

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	153 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. Promotes head development by inhibiting Wnt signaling during embryogenesis. Ref.3 Ref.4
Subcellular location	Secreted.
Tissue specificity	Expressed in adult liver, lung, heart, kidney and peritoneal fat. Ref.1 Ref.2
Developmental stage	Expressed both maternally and zygotically after the midblastula transition. Present both dorsally and ventrally at the beginning of neurulation. Expression is restricted to the developing heart at the tailbud stage. Ref.3 Ref.4
Sequence similarities	Belongs to the insulin family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Developmental protein Growth factor
PTM	Disulfide bond
Gene Ontology (GO)
Biological_process	head morphogenesis Inferred from direct assay Ref.4. Source: UniProtKB multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of Wnt receptor signaling pathway Inferred from direct assay Ref.4. Source: UniProtKB negative regulation of apoptotic process Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of release of cytochrome c from mitochondria Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	extracellular region Non-traceable author statement Ref.3. Source: UniProtKB extracellular space Inferred from electronic annotation. Source: InterPro
Molecular_function	growth factor activity Inferred from direct assay Ref.4. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – ?
	Propeptide	? – 48				PRO_0000015708
	Chain	49 – 118	70	Insulin-like growth factor I-A		PRO_0000015709
	Propeptide	119 – 153	35	E peptide		PRO_0000015710
Regions
	Region	49 – 77	29	B
	Region	78 – 89	12	C
	Region	90 – 110	21	A
	Region	111 – 118	8	D
Amino acid modifications
	Disulfide bond	54 ↔ 96		By similarity
	Disulfide bond	66 ↔ 109		By similarity
	Disulfide bond	95 ↔ 100		By similarity
Experimental info
	Sequence conflict	114	1	Q → P in AAA70330. Ref.1
	Sequence conflict	123	1	T → A in AAA70330. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P16501 [UniParc]. Last modified March 7, 2006. Version 2. Checksum: F83BC8AA17AFDBEE	FASTA	153	17,410
	10 20 30 40 50 60 METNNNLSTQ LFKCYFCDIL KLKMHKMSCI HLLYLVLCFL TLTHSAAAGP ETLCGAELVD 70 80 90 100 110 120 TLQFVCGDRG FYFSKPTGYG SNNRRSHHRG IVDECCFQSC DFRRLEMYCA PAKQAKSARS 130 140 150 VRTQRHTDMP KAQKEVHPKN TSRGNTGSRG FRM

References

[1]	"Evolution of insulin-like growth factor I (IGF-I): structure and expression of an IGF-I precursor from Xenopus laevis." Kajimoto Y., Rotwein P. Mol. Endocrinol. 4:217-226(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Liver.
[2]	"Evidence that Xenopus laevis contains two different nonallelic insulin-like growth factor-I genes." Shuldiner A.R., Nirula A., Scott L.A., Roth J. Biochem. Biophys. Res. Commun. 166:223-230(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 82-125, TISSUE SPECIFICITY.
[3]	"Neural and head induction by insulin-like growth factor signals." Pera E.M., Wessely O., Li S.-Y., De Robertis E.M. Dev. Cell 1:655-665(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DEVELOPMENTAL STAGE.
[4]	"The IGF pathway regulates head formation by inhibiting Wnt signaling in Xenopus." Richard-Parpaillon L., Heligon C., Chesnel F., Boujard D., Philpott A. Dev. Biol. 244:407-417(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DEVELOPMENTAL STAGE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M29857 mRNA. Translation: AAA70330.1.
PIR	A34049. A36079.
RefSeq	NP_001156865.1. NM_001163393.1.
UniGene	Xl.23602.
3D structure databases
ProteinModelPortal	P16501.
SMR	P16501. Positions 49-118.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	378699.
KEGG	xla:378699.
Organism-specific databases
CTD	3479.
Xenbase	XB-GENE-6252361. igf1.
Phylogenomic databases
HOVERGEN	HBG006137.
KO	K05459.
Family and domain databases
Gene3D	1.10.100.10. 1 hit.
InterPro	IPR022341. IGF-I. IPR016179. Insulin-like. IPR022350. Insulin-like_growth_factor. IPR022353. Insulin_CS. IPR022352. Insulin_family. [Graphical view]
Pfam	PF00049. Insulin. 1 hit. [Graphical view]
PRINTS	PR02002. INSLNLIKEGF. PR02005. INSLNLIKEGF1. PR00276. INSULINFAMLY.
SMART	SM00078. IlGF. 1 hit. [Graphical view]
SUPFAM	SSF56994. Insulin-like. 1 hit.
PROSITE	PS00262. INSULIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IGF1A_XENLA

Accession

Primary (citable) accession number: P16501

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	March 7, 2006
Last modified:	April 3, 2013
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program