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P16495

- NCAP_BUNYW

UniProt

P16495 - NCAP_BUNYW

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Protein

Nucleoprotein

Gene

N

Organism
Bunyamwera virus (BUNV)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Encapsidates the genome protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC) and serves as template for transcription and replication. Seems to participate in the nuclear relocalization of host PABP1, thereby inhibiting host cellular translation.1 Publication

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. suppression by virus of host gene expression Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction

Keywords - Ligandi

RNA-binding, Viral nucleoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoprotein
Alternative name(s):
Nucleocapsid protein
Short name:
Protein N
Gene namesi
Name:N
OrganismiBunyamwera virus (BUNV)
Taxonomic identifieri35304 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesBunyaviridaeOrthobunyavirus
Virus hostiAedes aegypti (Yellowfever mosquito) (Culex aegypti) [TaxID: 7159]
Homo sapiens (Human) [TaxID: 9606]
ProteomesiUP000002476: Genome

Subcellular locationi

Virion
Note: Internal protein of virus particle.

GO - Cellular componenti

  1. helical viral capsid Source: UniProtKB-KW
  2. ribonucleoprotein complex Source: UniProtKB-KW
  3. viral nucleocapsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Helical capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 233233NucleoproteinPRO_0000221992Add
BLAST

Interactioni

Subunit structurei

Homomultimerizes to form the nucleocapsid (By similarity). Binds to viral genomic RNA (By similarity). Interacts with host PABP1.By similarity1 Publication

Structurei

Secondary structure

233
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi1 – 66Combined sources
Helixi19 – 2911Combined sources
Beta strandi31 – 333Combined sources
Helixi36 – 449Combined sources
Helixi46 – 5510Combined sources
Beta strandi59 – 657Combined sources
Beta strandi68 – 736Combined sources
Helixi78 – 803Combined sources
Helixi92 – 10918Combined sources
Helixi112 – 12110Combined sources
Helixi125 – 1295Combined sources
Helixi134 – 1363Combined sources
Helixi138 – 1436Combined sources
Helixi148 – 1514Combined sources
Turni152 – 1576Combined sources
Helixi158 – 16710Combined sources
Turni168 – 1703Combined sources
Helixi174 – 1774Combined sources
Helixi180 – 1823Combined sources
Helixi190 – 1967Combined sources
Helixi198 – 21013Combined sources
Beta strandi215 – 2173Combined sources
Helixi220 – 2278Combined sources
Turni228 – 2303Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3ZLAX-ray3.20A/B/C/D/E/F/G/H1-233[»]
4IJSX-ray3.20A/B/C/D2-233[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR001784. Bunya_nucleocap.
[Graphical view]
PfamiPF00952. Bunya_nucleocap. 1 hit.
[Graphical view]
PIRSFiPIRSF003947. N_OrthobunV. 1 hit.
ProDomiPD001909. Bunya_nucleocap. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. Align

Isoform N (identifier: P16495-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIELEFHDVA ANTSSTFDPE VAYANFKRVH TTGLSYDHIR IFYIKGREIK
60 70 80 90 100
TSLAKRSEWE VTLNLGGWKI TVYNTNFPGN RNNPVPDDGL TLHRLSGFLA
110 120 130 140 150
RYLLEKMLKV SEPEKLIIKS KIINPLAEKN GITWNDGEEV YLSFFPGSEM
160 170 180 190 200
FLGTFRFYPL AIGIYKVQRK EMEPKYLEKT MRQRYMGLEA ATWTVSKLTE
210 220 230
VQSALTVVSS LGWKKTNVSA AARDFLAKFG INM
Length:233
Mass (Da):26,664
Last modified:August 1, 1990 - v1
Checksum:iD44C80C93FA8A066
GO
Isoform NSS (identifier: P16494-1) [UniParc]FASTAAdd to Basket

The sequence of this isoform can be found in the external entry P16494.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by alternative initiation in the N gene, but encoded on another frame.

Length:101
Mass (Da):11,024
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00353 Genomic RNA. Translation: BAA00261.1.
PIRiA31365. VHVUBV.
RefSeqiNP_047213.1. NC_001927.1. [P16495-1]

Genome annotation databases

GeneIDi2648217.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00353 Genomic RNA. Translation: BAA00261.1 .
PIRi A31365. VHVUBV.
RefSeqi NP_047213.1. NC_001927.1. [P16495-1 ]

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3ZLA X-ray 3.20 A/B/C/D/E/F/G/H 1-233 [» ]
4IJS X-ray 3.20 A/B/C/D 2-233 [» ]
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 2648217.

Family and domain databases

InterProi IPR001784. Bunya_nucleocap.
[Graphical view ]
Pfami PF00952. Bunya_nucleocap. 1 hit.
[Graphical view ]
PIRSFi PIRSF003947. N_OrthobunV. 1 hit.
ProDomi PD001909. Bunya_nucleocap. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence analysis of the small (S) RNA segment of Bunyamwera virus, the prototype of the family Bunyaviridae."
    Elliott R.M.
    J. Gen. Virol. 70:1281-1285(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Bunyamwera orthobunyavirus S-segment untranslated regions mediate poly(A) tail-independent translation."
    Blakqori G., van Knippenberg I., Elliott R.M.
    J. Virol. 83:3637-3646(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH HOST PABP1.

Entry informationi

Entry nameiNCAP_BUNYW
AccessioniPrimary (citable) accession number: P16495
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: November 26, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3