P16494 (NSS_BUNYW) Reviewed, UniProtKB/Swiss-Prot
Last modified July 24, 2013. Version 51. History...
Names and origin
|Protein names||Recommended name:|
Non-structural protein NS-S
|Organism||Bunyamwera virus (BUNV) [Reference proteome]|
|Taxonomic identifier||35304 [NCBI]|
|Taxonomic lineage||Viruses › ssRNA negative-strand viruses › Bunyaviridae › Orthobunyavirus|
|Virus host||Aedes aegypti (Yellowfever mosquito) (Culex aegypti) [TaxID: 7159]|
Homo sapiens (Human) [TaxID: 9606]
|Sequence length||101 AA.|
|Protein existence||Inferred from homology|
General annotation (Comments)
Inhibits host transcriptional machinery, by producing modifications to the phosphorylation state of the C-terminal domain (CTD) of RNA polymerase II. Inhibits phosphorylation at serine 2 in the heptapeptide repeat (YSPTSPS) of the CTD of RNA polymerase II, suggesting that the elongation step of transcription and/or 3'-end processing is prevented. Inhibition of host transcription machinery leads to shut off of host cell protein synthesis and inhibition of the host innate immune response. NSs also seems to be involved in the nuclear relocalization of host PABP1. Ref.2 Ref.3
Belongs to the orthobunyavirus NS-S protein family.
|Biological process||Host gene expression shutoff by virus|
Host transcription shutoff by virus
Inhibition of host RNA polymerase II by virus
|Coding sequence diversity||Alternative initiation|
|Technical term||Complete proteome|
|Gene Ontology (GO)|
|Biological_process||suppression by virus of host RNA polymerase II activity|
Inferred from electronic annotation. Source: UniProtKB-KW
|Complete GO annotation...|
|This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]|
|Isoform NSS (identifier: P16494-1) |
This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
|Note: Produced by alternative initiation in the N gene, but encoded on another frame.|
|Isoform N (identifier: P16495-1) |
The sequence of this isoform can be found in the external entry P16495.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Sequence annotation (Features)
|Feature key||Position(s)||Length||Description||Graphical view||Feature identifier|
|Chain||1 – 101||101||Non-structural protein NS-S||PRO_0000221976|
|||"Nucleotide sequence analysis of the small (S) RNA segment of Bunyamwera virus, the prototype of the family Bunyaviridae."|
J. Gen. Virol. 70:1281-1285(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
|||"Inhibition of RNA polymerase II phosphorylation by a viral interferon antagonist."|
Thomas D., Blakqori G., Wagner V., Banholzer M., Kessler N., Elliott R.M., Haller O., Weber F.
J. Biol. Chem. 279:31471-31477(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
|||"Bunyamwera orthobunyavirus S-segment untranslated regions mediate poly(A) tail-independent translation."|
Blakqori G., van Knippenberg I., Elliott R.M.
J. Virol. 83:3637-3646(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
|D00353 Genomic RNA. Translation: BAA00262.1.|
|PIR||MNVUBV. B31365. |
|RefSeq||NP_047214.1. NC_001927.1. |
3D structure databases
Protocols and materials databases
Genome annotation databases
Family and domain databases
|InterPro||IPR000797. Bunya_NSs. |
|Pfam||PF01104. Bunya_NS-S. 1 hit. |
|PIRSF||PIRSF003954. NS-S_OrthobunV. 1 hit. |
|Accession||Primary (citable) accession number: P16494|
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Viral Protein Annotation Program|
Index of protein domains and families