ID   ORF2A_CMVFN             Reviewed;         857 AA.
AC   P16490;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1990, sequence version 1.
DT   16-JUN-2009, entry version 44.
DE   RecName: Full=RNA-directed RNA polymerase 2A;
DE            Short=protein 2A;
DE            EC=2.7.7.48;
GN   Name=RNA2; ORFNames=ORF2a;
OS   Cucumber mosaic virus (strain FNY) (CMV).
OC   Viruses; ssRNA positive-strand viruses, no DNA stage; Bromoviridae;
OC   Cucumovirus.
OX   NCBI_TaxID=12307;
OH   NCBI_TaxID=3663; Cucurbita pepo (Vegetable marrow) (Summer squash).
OH   NCBI_TaxID=4097; Nicotiana tabacum (Common tobacco).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   MEDLINE=88299943; PubMed=3404113;
RA   Rizzo T.M., Palukaitis P.;
RT   "Nucleotide sequence and evolutionary relationships of cucumber mosaic
RT   virus (CMV) strains: CMV RNA 2.";
RL   J. Gen. Virol. 69:1777-1787(1988).
CC   -!- FUNCTION: RNA-directed RNA polymerase.
CC   -!- CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate
CC       + RNA(n+1).
CC   -!- MISCELLANEOUS: The virus has a tripartite genome (RNA1, RNA2 and
CC       RNA3) and an encapsidated subgenomic RNA (RNA4) from which the
CC       coat protein is expressed. Virions are icosahedral, not enveloped,
CC       with 32 capsomers per nucleocapsid.
CC   -!- SIMILARITY: Belongs to the cucumoviruses/bromoviruses 2A family.
CC   -!- SIMILARITY: Contains 1 RdRp catalytic domain.
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DR   EMBL; D00355; BAA00263.1; -; Genomic_RNA.
DR   PIR; JQ0015; WMVXCU.
DR   RefSeq; NP_049324.1; -.
DR   GeneID; 962642; -.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006410; P:transcription, RNA-dependent; IEA:UniProtKB-KW.
DR   GO; GO:0019079; P:viral genome replication; IEA:InterPro.
DR   InterPro; IPR001788; RNA-dep_RNA_pol_vir-typ.
DR   InterPro; IPR007094; RNA_pol_PSvir.
DR   Pfam; PF00978; RdRP_2; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE   3: Inferred from homology;
KW   Nucleotide-binding; Nucleotidyltransferase; RNA replication;
KW   RNA-directed RNA polymerase; Transferase.
FT   CHAIN         1    857       RNA-directed RNA polymerase 2A.
FT                                /FTId=PRO_0000083273.
FT   DOMAIN      511    624       RdRp catalytic.
SQ   SEQUENCE   857 AA;  96710 MW;  5BBA030FE68D7B2B CRC64;
     MAFPAPAFSL ANLLNGSYGV DTPEDVERLR SEQREEAAAA CRNYRPLPAV DVSESVTEDA
     HSLRTPDGAP AEAVSDEFVT YGAEDYLEKS DDELLVAFET MVKPMRIGQL WCPAFNKCSF
     ISSIAMARAL LLAPRTSHRT MKCFEDLVAA IYTKSDFYYS EECEADDAQI DISSRDVPGY
     SFEPWSRTSG FEPPPICEAC DMIMYQCPCF DFNALKKSCA ERTFADDYVI EGLDGVVDNA
     TLLSNLGPFL VPVKCQYEKC PTPTIAIPPD LNRATDRVDI NLVQSICDST LPTHSNYDDS
     FHQVFVESAD YSIDLDHVRL RQSDLIAKIP DSGHMIPVLN TGSGHKRVGT TKEVLTAIKK
     RNADVPELGD SVNLSRLSKA VAERFFISYI NGNSLASSNF VNVVSNFHDY MEKWKSSGLS
     YDDLPDLHAE NLQFYDHMIK SDVKPVVSDT LNIDRPVPAT ITYHKKSITS QFSPLFTALF
     ERFQRCLRER IILPVGKISS LEMAGFDVKN KHCLEIDLSK FDKSQGEFHL LIQEHILNGL
     GCPAPITKWW CDFHRFSYIR DRRAGVGMPI SFQRRTGDAL TYFGNTIVTM AEFAWCYDTD
     QFEKLLFSGD DSLGFSLLPP VGDPSKFTTL FNMEAKVMEP AVPYICSKFL LSDEFGNTFS
     VPDPLREVQR LGTKKIPYSD NDEFLFAHFM SFVDRLKFLD RMSQSCIDQL SIFFELKYKK
     SGEEAALMLG AFKKYTANFQ SYKELYYSDR RQCELINSFC STEFRVERVN SNKQRKNYGI
     ERRCNDKRRT PTGSYGGGEE AETKVSQTES TGTRSQKSQR ESAFKSQTIP LPTVLSSGWF
     GTDRVMPPCE RGGVTRV
//