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P16474

- GRP78_YEAST

UniProt

P16474 - GRP78_YEAST

Protein

78 kDa glucose-regulated protein homolog

Gene

KAR2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 156 (01 Oct 2014)
      Sequence version 1 (01 Aug 1990)
      Previous versions | rss
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    Functioni

    Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER. Is required for secretory polypeptide translocation. May physically associate with SEC63 protein in the endoplasmic reticulum and this interaction may be regulated by ATP hydrolysis.1 Publication

    GO - Molecular functioni

    1. ATPase activity Source: SGD
    2. ATP binding Source: UniProtKB-KW
    3. protein binding Source: IntAct
    4. unfolded protein binding Source: SGD

    GO - Biological processi

    1. ATP catabolic process Source: GOC
    2. ER-associated ubiquitin-dependent protein catabolic process Source: SGD
    3. karyogamy involved in conjugation with cellular fusion Source: SGD
    4. posttranslational protein targeting to membrane, translocation Source: SGD
    5. response to unfolded protein Source: SGD
    6. SRP-dependent cotranslational protein targeting to membrane, translocation Source: SGD

    Keywords - Biological processi

    Stress response

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31501-MONOMER.
    ReactomeiREACT_108597. ATF6-alpha activates chaperone genes.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    78 kDa glucose-regulated protein homolog
    Short name:
    GRP-78
    Alternative name(s):
    Immunoglobulin heavy chain-binding protein homolog
    Short name:
    BiP
    Gene namesi
    Name:KAR2
    Synonyms:GRP78, SSD1
    Ordered Locus Names:YJL034W
    ORF Names:J1248
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJL034w.
    SGDiS000003571. KAR2.

    Subcellular locationi

    Endoplasmic reticulum lumen 1 PublicationPROSITE-ProRule annotation

    GO - Cellular componenti

    1. endoplasmic reticulum Source: SGD
    2. luminal surveillance complex Source: SGD

    Keywords - Cellular componenti

    Endoplasmic reticulum

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 4242Sequence AnalysisAdd
    BLAST
    Chaini43 – 68264078 kDa glucose-regulated protein homologPRO_0000013587Add
    BLAST

    Proteomic databases

    MaxQBiP16474.
    PaxDbiP16474.
    PeptideAtlasiP16474.
    PRIDEiP16474.

    2D gel databases

    SWISS-2DPAGEP16474.

    Expressioni

    Gene expression databases

    GenevestigatoriP16474.

    Interactioni

    Subunit structurei

    Interacts with EPS1, PDI1 and YOS9.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    IDP1P219542EBI-7876,EBI-8898
    YOS9Q992202EBI-7876,EBI-34938

    Protein-protein interaction databases

    BioGridi33726. 443 interactions.
    DIPiDIP-2392N.
    IntActiP16474. 99 interactions.
    MINTiMINT-506436.
    STRINGi4932.YJL034W.

    Structurei

    Secondary structure

    1
    682
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi53 – 575
    Beta strandi59 – 679
    Beta strandi72 – 743
    Beta strandi82 – 854
    Beta strandi88 – 903
    Beta strandi95 – 984
    Helixi99 – 1035
    Helixi105 – 1073
    Helixi109 – 1113
    Helixi116 – 1183
    Turni119 – 1213
    Helixi127 – 1337
    Beta strandi137 – 1437
    Beta strandi146 – 16015
    Helixi162 – 18120
    Beta strandi187 – 1926
    Helixi198 – 21013
    Beta strandi214 – 2207
    Helixi221 – 2288
    Turni229 – 2324
    Beta strandi238 – 2458
    Beta strandi250 – 2589
    Beta strandi261 – 27010
    Helixi275 – 29420
    Helixi298 – 3003
    Helixi302 – 31817
    Turni319 – 3213
    Beta strandi323 – 33311
    Beta strandi336 – 3438
    Helixi344 – 35714
    Helixi359 – 36911
    Helixi373 – 3753
    Beta strandi378 – 3836
    Helixi384 – 3874
    Helixi389 – 39810
    Turni399 – 4013
    Turni410 – 4123
    Helixi413 – 42513
    Beta strandi444 – 4485
    Turni449 – 4513
    Beta strandi452 – 4576
    Beta strandi462 – 47110
    Beta strandi481 – 4899
    Helixi493 – 4953
    Beta strandi496 – 5049
    Beta strandi517 – 5237
    Beta strandi527 – 5359
    Turni536 – 5383
    Beta strandi541 – 5477
    Helixi555 – 56713
    Helixi569 – 58012

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3H0XX-ray1.92A438-586[»]
    3QFPX-ray2.26A43-426[»]
    3QFUX-ray1.80A43-426[»]
    3QMLX-ray2.31A/B43-426[»]
    ProteinModelPortaliP16474.
    SMRiP16474. Positions 48-621.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP16474.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi679 – 6824Prevents secretion from ER

    Sequence similaritiesi

    Belongs to the heat shock protein 70 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0443.
    GeneTreeiENSGT00750000117836.
    HOGENOMiHOG000228135.
    KOiK09490.
    OMAiHITITSK.
    OrthoDBiEOG728916.

    Family and domain databases

    Gene3Di1.20.1270.10. 1 hit.
    2.60.34.10. 1 hit.
    InterProiIPR018181. Heat_shock_70_CS.
    IPR029048. HSP70_C.
    IPR029047. HSP70_peptide-bd.
    IPR013126. Hsp_70_fam.
    [Graphical view]
    PfamiPF00012. HSP70. 1 hit.
    [Graphical view]
    PRINTSiPR00301. HEATSHOCK70.
    SUPFAMiSSF100920. SSF100920. 1 hit.
    SSF100934. SSF100934. 1 hit.
    PROSITEiPS00014. ER_TARGET. 1 hit.
    PS00297. HSP70_1. 1 hit.
    PS00329. HSP70_2. 1 hit.
    PS01036. HSP70_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P16474-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFFNRLSAGK LLVPLSVVLY ALFVVILPLQ NSFHSSNVLV RGADDVENYG    50
    TVIGIDLGTT YSCVAVMKNG KTEILANEQG NRITPSYVAF TDDERLIGDA 100
    AKNQVAANPQ NTIFDIKRLI GLKYNDRSVQ KDIKHLPFNV VNKDGKPAVE 150
    VSVKGEKKVF TPEEISGMIL GKMKQIAEDY LGTKVTHAVV TVPAYFNDAQ 200
    RQATKDAGTI AGLNVLRIVN EPTAAAIAYG LDKSDKEHQI IVYDLGGGTF 250
    DVSLLSIENG VFEVQATSGD THLGGEDFDY KIVRQLIKAF KKKHGIDVSD 300
    NNKALAKLKR EAEKAKRALS SQMSTRIEID SFVDGIDLSE TLTRAKFEEL 350
    NLDLFKKTLK PVEKVLQDSG LEKKDVDDIV LVGGSTRIPK VQQLLESYFD 400
    GKKASKGINP DEAVAYGAAV QAGVLSGEEG VEDIVLLDVN ALTLGIETTG 450
    GVMTPLIKRN TAIPTKKSQI FSTAVDNQPT VMIKVYEGER AMSKDNNLLG 500
    KFELTGIPPA PRGVPQIEVT FALDANGILK VSATDKGTGK SESITITNDK 550
    GRLTQEEIDR MVEEAEKFAS EDASIKAKVE SRNKLENYAH SLKNQVNGDL 600
    GEKLEEEDKE TLLDAANDVL EWLDDNFETA IAEDFDEKFE SLSKVAYPIT 650
    SKLYGGADGS GAADYDDEDE DDDGDYFEHD EL 682
    Length:682
    Mass (Da):74,468
    Last modified:August 1, 1990 - v1
    Checksum:iA107BD03DF3F30D3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M25064 Genomic DNA. Translation: AAA34714.1.
    M25394 Genomic DNA. Translation: AAA34713.1.
    M31006 Genomic DNA. Translation: AAA34454.1.
    Z49309 Genomic DNA. Translation: CAA89325.1.
    BK006943 Genomic DNA. Translation: DAA08765.1.
    PIRiA32366. HHBYK2.
    RefSeqiNP_012500.3. NM_001181468.3.

    Genome annotation databases

    EnsemblFungiiYJL034W; YJL034W; YJL034W.
    GeneIDi853418.
    KEGGisce:YJL034W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M25064 Genomic DNA. Translation: AAA34714.1 .
    M25394 Genomic DNA. Translation: AAA34713.1 .
    M31006 Genomic DNA. Translation: AAA34454.1 .
    Z49309 Genomic DNA. Translation: CAA89325.1 .
    BK006943 Genomic DNA. Translation: DAA08765.1 .
    PIRi A32366. HHBYK2.
    RefSeqi NP_012500.3. NM_001181468.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3H0X X-ray 1.92 A 438-586 [» ]
    3QFP X-ray 2.26 A 43-426 [» ]
    3QFU X-ray 1.80 A 43-426 [» ]
    3QML X-ray 2.31 A/B 43-426 [» ]
    ProteinModelPortali P16474.
    SMRi P16474. Positions 48-621.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33726. 443 interactions.
    DIPi DIP-2392N.
    IntActi P16474. 99 interactions.
    MINTi MINT-506436.
    STRINGi 4932.YJL034W.

    2D gel databases

    SWISS-2DPAGE P16474.

    Proteomic databases

    MaxQBi P16474.
    PaxDbi P16474.
    PeptideAtlasi P16474.
    PRIDEi P16474.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJL034W ; YJL034W ; YJL034W .
    GeneIDi 853418.
    KEGGi sce:YJL034W.

    Organism-specific databases

    CYGDi YJL034w.
    SGDi S000003571. KAR2.

    Phylogenomic databases

    eggNOGi COG0443.
    GeneTreei ENSGT00750000117836.
    HOGENOMi HOG000228135.
    KOi K09490.
    OMAi HITITSK.
    OrthoDBi EOG728916.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31501-MONOMER.
    Reactomei REACT_108597. ATF6-alpha activates chaperone genes.

    Miscellaneous databases

    EvolutionaryTracei P16474.
    NextBioi 973939.
    PROi P16474.

    Gene expression databases

    Genevestigatori P16474.

    Family and domain databases

    Gene3Di 1.20.1270.10. 1 hit.
    2.60.34.10. 1 hit.
    InterProi IPR018181. Heat_shock_70_CS.
    IPR029048. HSP70_C.
    IPR029047. HSP70_peptide-bd.
    IPR013126. Hsp_70_fam.
    [Graphical view ]
    Pfami PF00012. HSP70. 1 hit.
    [Graphical view ]
    PRINTSi PR00301. HEATSHOCK70.
    SUPFAMi SSF100920. SSF100920. 1 hit.
    SSF100934. SSF100934. 1 hit.
    PROSITEi PS00014. ER_TARGET. 1 hit.
    PS00297. HSP70_1. 1 hit.
    PS00329. HSP70_2. 1 hit.
    PS01036. HSP70_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "KAR2, a karyogamy gene, is the yeast homolog of the mammalian BiP/GRP78 gene."
      Rose M.D., Misra L.M., Vogel J.P.
      Cell 57:1211-1221(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION.
    2. "An essential member of the HSP70 gene family of Saccharomyces cerevisiae is homologous to immunoglobulin heavy chain binding protein."
      Nicholson R.C., Williams D.B., Moran L.A.
      Proc. Natl. Acad. Sci. U.S.A. 87:1159-1163(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: LL20.
    3. "S. cerevisiae encodes an essential protein homologous in sequence and function to mammalian BiP."
      Normington K., Kohno K., Kozutsumi Y., Gething M.J., Sambrook J.
      Cell 57:1223-1236(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Interactions among yeast protein-disulfide isomerase proteins and endoplasmic reticulum chaperone proteins influence their activities."
      Kimura T., Hosoda Y., Sato Y., Kitamura Y., Ikeda T., Horibe T., Kikuchi M.
      J. Biol. Chem. 280:31438-31441(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH EPS1 AND PDI1.
    8. "A luminal surveillance complex that selects misfolded glycoproteins for ER-associated degradation."
      Denic V., Quan E.M., Weissman J.S.
      Cell 126:349-359(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH YOS9.

    Entry informationi

    Entry nameiGRP78_YEAST
    AccessioniPrimary (citable) accession number: P16474
    Secondary accession number(s): D6VWE9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: August 1, 1990
    Last modified: October 1, 2014
    This is version 156 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 337000 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3