Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P16474 (GRP78_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 154. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
78 kDa glucose-regulated protein homolog

Short name=GRP-78
Alternative name(s):
Immunoglobulin heavy chain-binding protein homolog
Short name=BiP
Gene names
Name:KAR2
Synonyms:GRP78, SSD1
Ordered Locus Names:YJL034W
ORF Names:J1248
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length682 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER. Is required for secretory polypeptide translocation. May physically associate with SEC63 protein in the endoplasmic reticulum and this interaction may be regulated by ATP hydrolysis. Ref.7

Subunit structure

Interacts with EPS1 and PDI1. Ref.7

Subcellular location

Endoplasmic reticulum lumen Ref.1.

Miscellaneous

Present with 337000 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the heat shock protein 70 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

IDP1P219542EBI-7876,EBI-8898
YOS9Q992202EBI-7876,EBI-34938

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 4242 Potential
Chain43 – 68264078 kDa glucose-regulated protein homolog
PRO_0000013587

Regions

Motif679 – 6824Prevents secretion from ER

Secondary structure

.......................................................................................... 682
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P16474 [UniParc].

Last modified August 1, 1990. Version 1.
Checksum: A107BD03DF3F30D3

FASTA68274,468
        10         20         30         40         50         60 
MFFNRLSAGK LLVPLSVVLY ALFVVILPLQ NSFHSSNVLV RGADDVENYG TVIGIDLGTT 

        70         80         90        100        110        120 
YSCVAVMKNG KTEILANEQG NRITPSYVAF TDDERLIGDA AKNQVAANPQ NTIFDIKRLI 

       130        140        150        160        170        180 
GLKYNDRSVQ KDIKHLPFNV VNKDGKPAVE VSVKGEKKVF TPEEISGMIL GKMKQIAEDY 

       190        200        210        220        230        240 
LGTKVTHAVV TVPAYFNDAQ RQATKDAGTI AGLNVLRIVN EPTAAAIAYG LDKSDKEHQI 

       250        260        270        280        290        300 
IVYDLGGGTF DVSLLSIENG VFEVQATSGD THLGGEDFDY KIVRQLIKAF KKKHGIDVSD 

       310        320        330        340        350        360 
NNKALAKLKR EAEKAKRALS SQMSTRIEID SFVDGIDLSE TLTRAKFEEL NLDLFKKTLK 

       370        380        390        400        410        420 
PVEKVLQDSG LEKKDVDDIV LVGGSTRIPK VQQLLESYFD GKKASKGINP DEAVAYGAAV 

       430        440        450        460        470        480 
QAGVLSGEEG VEDIVLLDVN ALTLGIETTG GVMTPLIKRN TAIPTKKSQI FSTAVDNQPT 

       490        500        510        520        530        540 
VMIKVYEGER AMSKDNNLLG KFELTGIPPA PRGVPQIEVT FALDANGILK VSATDKGTGK 

       550        560        570        580        590        600 
SESITITNDK GRLTQEEIDR MVEEAEKFAS EDASIKAKVE SRNKLENYAH SLKNQVNGDL 

       610        620        630        640        650        660 
GEKLEEEDKE TLLDAANDVL EWLDDNFETA IAEDFDEKFE SLSKVAYPIT SKLYGGADGS 

       670        680 
GAADYDDEDE DDDGDYFEHD EL 

« Hide

References

« Hide 'large scale' references
[1]"KAR2, a karyogamy gene, is the yeast homolog of the mammalian BiP/GRP78 gene."
Rose M.D., Misra L.M., Vogel J.P.
Cell 57:1211-1221(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION.
[2]"An essential member of the HSP70 gene family of Saccharomyces cerevisiae is homologous to immunoglobulin heavy chain binding protein."
Nicholson R.C., Williams D.B., Moran L.A.
Proc. Natl. Acad. Sci. U.S.A. 87:1159-1163(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: LL20.
[3]"S. cerevisiae encodes an essential protein homologous in sequence and function to mammalian BiP."
Normington K., Kohno K., Kozutsumi Y., Gething M.J., Sambrook J.
Cell 57:1223-1236(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Interactions among yeast protein-disulfide isomerase proteins and endoplasmic reticulum chaperone proteins influence their activities."
Kimura T., Hosoda Y., Sato Y., Kitamura Y., Ikeda T., Horibe T., Kikuchi M.
J. Biol. Chem. 280:31438-31441(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH EPS1 AND PDI1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M25064 Genomic DNA. Translation: AAA34714.1.
M25394 Genomic DNA. Translation: AAA34713.1.
M31006 Genomic DNA. Translation: AAA34454.1.
Z49309 Genomic DNA. Translation: CAA89325.1.
BK006943 Genomic DNA. Translation: DAA08765.1.
PIRHHBYK2. A32366.
RefSeqNP_012500.3. NM_001181468.3.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3H0XX-ray1.92A438-586[»]
3QFPX-ray2.26A43-426[»]
3QFUX-ray1.80A43-426[»]
3QMLX-ray2.31A/B43-426[»]
ProteinModelPortalP16474.
SMRP16474. Positions 48-621.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33726. 443 interactions.
DIPDIP-2392N.
IntActP16474. 99 interactions.
MINTMINT-506436.
STRING4932.YJL034W.

2D gel databases

SWISS-2DPAGEP16474.

Proteomic databases

MaxQBP16474.
PaxDbP16474.
PeptideAtlasP16474.
PRIDEP16474.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJL034W; YJL034W; YJL034W.
GeneID853418.
KEGGsce:YJL034W.

Organism-specific databases

CYGDYJL034w.
SGDS000003571. KAR2.

Phylogenomic databases

eggNOGCOG0443.
GeneTreeENSGT00750000117836.
HOGENOMHOG000228135.
KOK09490.
OMAHITITSK.
OrthoDBEOG728916.

Enzyme and pathway databases

BioCycYEAST:G3O-31501-MONOMER.

Gene expression databases

GenevestigatorP16474.

Family and domain databases

Gene3D1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
InterProIPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamPF00012. HSP70. 1 hit.
[Graphical view]
PRINTSPR00301. HEATSHOCK70.
SUPFAMSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
PROSITEPS00014. ER_TARGET. 1 hit.
PS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP16474.
NextBio973939.
PROP16474.

Entry information

Entry nameGRP78_YEAST
AccessionPrimary (citable) accession number: P16474
Secondary accession number(s): D6VWE9
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: June 11, 2014
This is version 154 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references