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P16474

- GRP78_YEAST

UniProt

P16474 - GRP78_YEAST

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Protein

78 kDa glucose-regulated protein homolog

Gene

KAR2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER. Is required for secretory polypeptide translocation. May physically associate with SEC63 protein in the endoplasmic reticulum and this interaction may be regulated by ATP hydrolysis.1 Publication

GO - Molecular functioni

  1. ATPase activity Source: SGD
  2. ATP binding Source: UniProtKB-KW
  3. unfolded protein binding Source: SGD

GO - Biological processi

  1. ATP catabolic process Source: GOC
  2. ER-associated ubiquitin-dependent protein catabolic process Source: SGD
  3. karyogamy involved in conjugation with cellular fusion Source: SGD
  4. posttranslational protein targeting to membrane, translocation Source: SGD
  5. response to unfolded protein Source: SGD
  6. SRP-dependent cotranslational protein targeting to membrane, translocation Source: SGD
Complete GO annotation...

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31501-MONOMER.
ReactomeiREACT_108597. ATF6-alpha activates chaperone genes.

Names & Taxonomyi

Protein namesi
Recommended name:
78 kDa glucose-regulated protein homolog
Short name:
GRP-78
Alternative name(s):
Immunoglobulin heavy chain-binding protein homolog
Short name:
BiP
Gene namesi
Name:KAR2
Synonyms:GRP78, SSD1
Ordered Locus Names:YJL034W
ORF Names:J1248
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJL034w.
SGDiS000003571. KAR2.

Subcellular locationi

Endoplasmic reticulum lumen 1 PublicationPROSITE-ProRule annotation

GO - Cellular componenti

  1. endoplasmic reticulum Source: SGD
  2. luminal surveillance complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4242Sequence AnalysisAdd
BLAST
Chaini43 – 68264078 kDa glucose-regulated protein homologPRO_0000013587Add
BLAST

Proteomic databases

MaxQBiP16474.
PaxDbiP16474.
PeptideAtlasiP16474.
PRIDEiP16474.

2D gel databases

SWISS-2DPAGEP16474.

Expressioni

Gene expression databases

GenevestigatoriP16474.

Interactioni

Subunit structurei

Interacts with EPS1, PDI1 and YOS9.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
IDP1P219542EBI-7876,EBI-8898
YOS9Q992202EBI-7876,EBI-34938

Protein-protein interaction databases

BioGridi33726. 443 interactions.
DIPiDIP-2392N.
IntActiP16474. 99 interactions.
MINTiMINT-506436.
STRINGi4932.YJL034W.

Structurei

Secondary structure

1
682
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi53 – 575Combined sources
Beta strandi59 – 679Combined sources
Beta strandi72 – 743Combined sources
Beta strandi82 – 854Combined sources
Beta strandi88 – 903Combined sources
Beta strandi95 – 984Combined sources
Helixi99 – 1035Combined sources
Helixi105 – 1073Combined sources
Helixi109 – 1113Combined sources
Helixi116 – 1183Combined sources
Turni119 – 1213Combined sources
Helixi127 – 1337Combined sources
Beta strandi137 – 1437Combined sources
Beta strandi146 – 16015Combined sources
Helixi162 – 18120Combined sources
Beta strandi187 – 1926Combined sources
Helixi198 – 21013Combined sources
Beta strandi214 – 2207Combined sources
Helixi221 – 2288Combined sources
Turni229 – 2324Combined sources
Beta strandi238 – 2458Combined sources
Beta strandi250 – 2589Combined sources
Beta strandi261 – 27010Combined sources
Helixi275 – 29420Combined sources
Helixi298 – 3003Combined sources
Helixi302 – 31817Combined sources
Turni319 – 3213Combined sources
Beta strandi323 – 33311Combined sources
Beta strandi336 – 3438Combined sources
Helixi344 – 35714Combined sources
Helixi359 – 36911Combined sources
Helixi373 – 3753Combined sources
Beta strandi378 – 3836Combined sources
Helixi384 – 3874Combined sources
Helixi389 – 39810Combined sources
Turni399 – 4013Combined sources
Turni410 – 4123Combined sources
Helixi413 – 42513Combined sources
Beta strandi444 – 4485Combined sources
Turni449 – 4513Combined sources
Beta strandi452 – 4576Combined sources
Beta strandi462 – 47110Combined sources
Beta strandi481 – 4899Combined sources
Helixi493 – 4953Combined sources
Beta strandi496 – 5049Combined sources
Beta strandi517 – 5237Combined sources
Beta strandi527 – 5359Combined sources
Turni536 – 5383Combined sources
Beta strandi541 – 5477Combined sources
Helixi555 – 56713Combined sources
Helixi569 – 58012Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3H0XX-ray1.92A438-586[»]
3QFPX-ray2.26A43-426[»]
3QFUX-ray1.80A43-426[»]
3QMLX-ray2.31A/B43-426[»]
ProteinModelPortaliP16474.
SMRiP16474. Positions 48-656.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP16474.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi679 – 6824Prevents secretion from ER

Sequence similaritiesi

Belongs to the heat shock protein 70 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0443.
GeneTreeiENSGT00770000120648.
HOGENOMiHOG000228135.
InParanoidiP16474.
KOiK09490.
OMAiHITITSK.
OrthoDBiEOG728916.

Family and domain databases

Gene3Di1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
InterProiIPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamiPF00012. HSP70. 1 hit.
[Graphical view]
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P16474-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFFNRLSAGK LLVPLSVVLY ALFVVILPLQ NSFHSSNVLV RGADDVENYG
60 70 80 90 100
TVIGIDLGTT YSCVAVMKNG KTEILANEQG NRITPSYVAF TDDERLIGDA
110 120 130 140 150
AKNQVAANPQ NTIFDIKRLI GLKYNDRSVQ KDIKHLPFNV VNKDGKPAVE
160 170 180 190 200
VSVKGEKKVF TPEEISGMIL GKMKQIAEDY LGTKVTHAVV TVPAYFNDAQ
210 220 230 240 250
RQATKDAGTI AGLNVLRIVN EPTAAAIAYG LDKSDKEHQI IVYDLGGGTF
260 270 280 290 300
DVSLLSIENG VFEVQATSGD THLGGEDFDY KIVRQLIKAF KKKHGIDVSD
310 320 330 340 350
NNKALAKLKR EAEKAKRALS SQMSTRIEID SFVDGIDLSE TLTRAKFEEL
360 370 380 390 400
NLDLFKKTLK PVEKVLQDSG LEKKDVDDIV LVGGSTRIPK VQQLLESYFD
410 420 430 440 450
GKKASKGINP DEAVAYGAAV QAGVLSGEEG VEDIVLLDVN ALTLGIETTG
460 470 480 490 500
GVMTPLIKRN TAIPTKKSQI FSTAVDNQPT VMIKVYEGER AMSKDNNLLG
510 520 530 540 550
KFELTGIPPA PRGVPQIEVT FALDANGILK VSATDKGTGK SESITITNDK
560 570 580 590 600
GRLTQEEIDR MVEEAEKFAS EDASIKAKVE SRNKLENYAH SLKNQVNGDL
610 620 630 640 650
GEKLEEEDKE TLLDAANDVL EWLDDNFETA IAEDFDEKFE SLSKVAYPIT
660 670 680
SKLYGGADGS GAADYDDEDE DDDGDYFEHD EL
Length:682
Mass (Da):74,468
Last modified:August 1, 1990 - v1
Checksum:iA107BD03DF3F30D3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25064 Genomic DNA. Translation: AAA34714.1.
M25394 Genomic DNA. Translation: AAA34713.1.
M31006 Genomic DNA. Translation: AAA34454.1.
Z49309 Genomic DNA. Translation: CAA89325.1.
BK006943 Genomic DNA. Translation: DAA08765.1.
PIRiA32366. HHBYK2.
RefSeqiNP_012500.3. NM_001181468.3.

Genome annotation databases

EnsemblFungiiYJL034W; YJL034W; YJL034W.
GeneIDi853418.
KEGGisce:YJL034W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25064 Genomic DNA. Translation: AAA34714.1 .
M25394 Genomic DNA. Translation: AAA34713.1 .
M31006 Genomic DNA. Translation: AAA34454.1 .
Z49309 Genomic DNA. Translation: CAA89325.1 .
BK006943 Genomic DNA. Translation: DAA08765.1 .
PIRi A32366. HHBYK2.
RefSeqi NP_012500.3. NM_001181468.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3H0X X-ray 1.92 A 438-586 [» ]
3QFP X-ray 2.26 A 43-426 [» ]
3QFU X-ray 1.80 A 43-426 [» ]
3QML X-ray 2.31 A/B 43-426 [» ]
ProteinModelPortali P16474.
SMRi P16474. Positions 48-656.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33726. 443 interactions.
DIPi DIP-2392N.
IntActi P16474. 99 interactions.
MINTi MINT-506436.
STRINGi 4932.YJL034W.

2D gel databases

SWISS-2DPAGE P16474.

Proteomic databases

MaxQBi P16474.
PaxDbi P16474.
PeptideAtlasi P16474.
PRIDEi P16474.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJL034W ; YJL034W ; YJL034W .
GeneIDi 853418.
KEGGi sce:YJL034W.

Organism-specific databases

CYGDi YJL034w.
SGDi S000003571. KAR2.

Phylogenomic databases

eggNOGi COG0443.
GeneTreei ENSGT00770000120648.
HOGENOMi HOG000228135.
InParanoidi P16474.
KOi K09490.
OMAi HITITSK.
OrthoDBi EOG728916.

Enzyme and pathway databases

BioCyci YEAST:G3O-31501-MONOMER.
Reactomei REACT_108597. ATF6-alpha activates chaperone genes.

Miscellaneous databases

EvolutionaryTracei P16474.
NextBioi 973939.
PROi P16474.

Gene expression databases

Genevestigatori P16474.

Family and domain databases

Gene3Di 1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
InterProi IPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view ]
Pfami PF00012. HSP70. 1 hit.
[Graphical view ]
PRINTSi PR00301. HEATSHOCK70.
SUPFAMi SSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
PROSITEi PS00014. ER_TARGET. 1 hit.
PS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "KAR2, a karyogamy gene, is the yeast homolog of the mammalian BiP/GRP78 gene."
    Rose M.D., Misra L.M., Vogel J.P.
    Cell 57:1211-1221(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION.
  2. "An essential member of the HSP70 gene family of Saccharomyces cerevisiae is homologous to immunoglobulin heavy chain binding protein."
    Nicholson R.C., Williams D.B., Moran L.A.
    Proc. Natl. Acad. Sci. U.S.A. 87:1159-1163(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: LL20.
  3. "S. cerevisiae encodes an essential protein homologous in sequence and function to mammalian BiP."
    Normington K., Kohno K., Kozutsumi Y., Gething M.J., Sambrook J.
    Cell 57:1223-1236(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Interactions among yeast protein-disulfide isomerase proteins and endoplasmic reticulum chaperone proteins influence their activities."
    Kimura T., Hosoda Y., Sato Y., Kitamura Y., Ikeda T., Horibe T., Kikuchi M.
    J. Biol. Chem. 280:31438-31441(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH EPS1 AND PDI1.
  8. "A luminal surveillance complex that selects misfolded glycoproteins for ER-associated degradation."
    Denic V., Quan E.M., Weissman J.S.
    Cell 126:349-359(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH YOS9.

Entry informationi

Entry nameiGRP78_YEAST
AccessioniPrimary (citable) accession number: P16474
Secondary accession number(s): D6VWE9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: November 26, 2014
This is version 158 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 337000 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3