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Protein

DNA topoisomerase 1

Gene

TOP1

Organism
Rabbit fibroma virus (strain Kasza) (RFV) (Shope fibroma virus (strain Kasza))
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).By similarity

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei167Involved in religationBy similarity1
Active sitei273O-(3'-phospho-DNA)-tyrosine intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Isomerase, Topoisomerase

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1 (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase I
Gene namesi
Name:TOP1
ORF Names:s074R
OrganismiRabbit fibroma virus (strain Kasza) (RFV) (Shope fibroma virus (strain Kasza))
Taxonomic identifieri10272 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeLeporipoxvirus
Virus hostiOryctolagus cuniculus (Rabbit) [TaxID: 9986]
Proteomesi
  • UP000000868 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001452131 – 314DNA topoisomerase 1Add BLAST314

Proteomic databases

PRIDEiP16472

Expressioni

Keywords - Developmental stagei

Late protein

Structurei

3D structure databases

ProteinModelPortaliP16472
SMRiP16472
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the type IB topoisomerase family.Curated

Phylogenomic databases

OrthoDBiVOG090000FB

Family and domain databases

Gene3Di1.20.120.380, 1 hit
3.30.66.10, 1 hit
3.90.15.10, 1 hit
InterProiView protein in InterPro
IPR011010 DNA_brk_join_enz
IPR035447 DNA_topo_I_N
IPR001631 TopoI
IPR018521 TopoI_AS
IPR027362 TopoI_C
IPR014711 TopoI_cat_a-hlx-sub_euk
IPR013500 TopoI_cat_euk
IPR015346 TopoI_N_vir
PANTHERiPTHR10290 PTHR10290, 1 hit
PfamiView protein in Pfam
PF01028 Topoisom_I, 1 hit
PF09266 VirDNA-topo-I_N, 1 hit
PRINTSiPR00416 EUTPISMRASEI
SUPFAMiSSF55869 SSF55869, 1 hit
SSF56349 SSF56349, 1 hit
PROSITEiView protein in PROSITE
PS00176 TOPOISOMERASE_I_EUK, 1 hit

Sequencei

Sequence statusi: Complete.

P16472-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRAFTYKDGK LYEDKELTIP VHCSNPTYEI LKHVKIPSHL TDVIVYEQTY
60 70 80 90 100
EQSLSRLIFV GLDSKGRRQY FYGKMHVQRR NSARDTIFIK VHRVIDKIHK
110 120 130 140 150
FIDDTIEHKN DVLFQLGVFM LMETSFFIRM GKVKYLKEND TVGLLTLKNK
160 170 180 190 200
NIVRENRKIL IHFVGKDKII HNFTVHSSNR LYKPLLRLIG RKEPDSFLFH
210 220 230 240 250
KLSEKKVYKA VQQFGIRLKD LRTYGVNYTF LYNFWTNVKS LNPIPPIKKM
260 270 280 290 300
ISTSIKQTAD IVGHTPSISK RAYIANTVLE YLTHDSELIN TIRDISFDEF
310
IRLITDYITN TQTV
Length:314
Mass (Da):36,999
Last modified:August 1, 1990 - v1
Checksum:iAF602490AD148161
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31723 Genomic DNA Translation: AAA47228.1
AF170722 Genomic DNA Translation: AAF17956.1
PIRiA34769 ITVZKA
RefSeqiNP_051963.1, NC_001266.1

Genome annotation databases

GeneIDi1486917
KEGGivg:1486917

Similar proteinsi

Entry informationi

Entry nameiTOP1_RFVKA
AccessioniPrimary (citable) accession number: P16472
Secondary accession number(s): Q77PC2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: May 23, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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