Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P16472 (TOP1_RFVKA) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase 1

EC=5.99.1.2
Alternative name(s):
DNA topoisomerase I
Gene names
Name:TOP1
ORF Names:s074R
OrganismRabbit fibroma virus (strain Kasza) (RFV) (Shope fibroma virus (strain Kasza)) [Reference proteome]
Taxonomic identifier10272 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeLeporipoxvirus
Virus hostOryctolagus cuniculus (Rabbit) [TaxID: 9986]

Protein attributes

Sequence length314 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.

Sequence similarities

Belongs to the type IB topoisomerase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 314314DNA topoisomerase 1
PRO_0000145213

Sites

Active site2731O-(3'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

Sequence LengthMass (Da)Tools
P16472 [UniParc].

Last modified August 1, 1990. Version 1.
Checksum: AF602490AD148161

FASTA31436,999
        10         20         30         40         50         60 
MRAFTYKDGK LYEDKELTIP VHCSNPTYEI LKHVKIPSHL TDVIVYEQTY EQSLSRLIFV 

        70         80         90        100        110        120 
GLDSKGRRQY FYGKMHVQRR NSARDTIFIK VHRVIDKIHK FIDDTIEHKN DVLFQLGVFM 

       130        140        150        160        170        180 
LMETSFFIRM GKVKYLKEND TVGLLTLKNK NIVRENRKIL IHFVGKDKII HNFTVHSSNR 

       190        200        210        220        230        240 
LYKPLLRLIG RKEPDSFLFH KLSEKKVYKA VQQFGIRLKD LRTYGVNYTF LYNFWTNVKS 

       250        260        270        280        290        300 
LNPIPPIKKM ISTSIKQTAD IVGHTPSISK RAYIANTVLE YLTHDSELIN TIRDISFDEF 

       310 
IRLITDYITN TQTV 

« Hide

References

« Hide 'large scale' references
[1]"Identification and DNA sequence of the Shope fibroma virus DNA topoisomerase gene."
Upton C., Opgenorth A., Traktman P., McFadden G.
Virology 176:439-447(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Sequence and analysis of a portion of the genomes of Shope fibroma virus and malignant rabbit fibroma virus that is important for viral replication in lymphocytes."
Strayer D.S., Jerng H.H., O'Connor K.
Virology 185:585-595(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Shope fibroma virus DNA topoisomerase catalyses holliday junction resolution and hairpin formation in vitro."
Palaniyar N., Gerasimopoulos E., Evans D.H.
J. Mol. Biol. 287:9-20(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"The complete genome sequence of shope (Rabbit) fibroma virus."
Willer D.O., McFadden G., Evans D.H.
Virology 264:319-343(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M31723 Genomic DNA. Translation: AAA47228.1.
AF170722 Genomic DNA. Translation: AAF17956.1.
PIRITVZKA. A34769.
RefSeqNP_051963.1. NC_001266.1.

3D structure databases

ProteinModelPortalP16472.
SMRP16472. Positions 1-311.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1486917.

Family and domain databases

Gene3D1.20.120.380. 1 hit.
3.30.66.10. 1 hit.
3.90.15.10. 1 hit.
InterProIPR011010. DNA_brk_join_enz.
IPR001631. TopoI.
IPR018521. TopoI_AS.
IPR014711. TopoI_cat_a-hlx-sub_euk.
IPR027362. TopoI_cat_dom2.
IPR013500. TopoI_cat_euk.
IPR015346. TopoI_N_vir.
[Graphical view]
PfamPF01028. Topoisom_I. 1 hit.
PF09266. VirDNA-topo-I_N. 1 hit.
[Graphical view]
PRINTSPR00416. EUTPISMRASEI.
SUPFAMSSF56349. SSF56349. 1 hit.
PROSITEPS00176. TOPOISOMERASE_I_EUK. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTOP1_RFVKA
AccessionPrimary (citable) accession number: P16472
Secondary accession number(s): Q77PC2
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: July 9, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families