P16472 (TOP1_RFVKA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA topoisomerase 1
EC=5.99.1.2

Alternative name(s):
DNA topoisomerase I

Gene names

Name:	TOP1
ORF Names:	s074R

Organism

Rabbit fibroma virus (strain Kasza) (RFV) (Shope fibroma virus (strain Kasza))

Taxonomic identifier

10272 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Leporipoxvirus

Virus host

Oryctolagus cuniculus (Rabbit) [TaxID: 9986]

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
Sequence similarities	Belongs to the type IB topoisomerase family.

Ontologies

Keywords
Developmental stage	Late protein
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	DNA topoisomerase 1		PRO_0000145213
Sites
	Active site	273	1	O-(3'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P16472 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: AF602490AD148161	FASTA	314	36,999
	10 20 30 40 50 60 MRAFTYKDGK LYEDKELTIP VHCSNPTYEI LKHVKIPSHL TDVIVYEQTY EQSLSRLIFV 70 80 90 100 110 120 GLDSKGRRQY FYGKMHVQRR NSARDTIFIK VHRVIDKIHK FIDDTIEHKN DVLFQLGVFM 130 140 150 160 170 180 LMETSFFIRM GKVKYLKEND TVGLLTLKNK NIVRENRKIL IHFVGKDKII HNFTVHSSNR 190 200 210 220 230 240 LYKPLLRLIG RKEPDSFLFH KLSEKKVYKA VQQFGIRLKD LRTYGVNYTF LYNFWTNVKS 250 260 270 280 290 300 LNPIPPIKKM ISTSIKQTAD IVGHTPSISK RAYIANTVLE YLTHDSELIN TIRDISFDEF 310 IRLITDYITN TQTV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and DNA sequence of the Shope fibroma virus DNA topoisomerase gene." Upton C., Opgenorth A., Traktman P., McFadden G. Virology 176:439-447(1990) [PubMed: 2161144] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence and analysis of a portion of the genomes of Shope fibroma virus and malignant rabbit fibroma virus that is important for viral replication in lymphocytes." Strayer D.S., Jerng H.H., O'Connor K. Virology 185:585-595(1991) [PubMed: 1660196] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Shope fibroma virus DNA topoisomerase catalyses holliday junction resolution and hairpin formation in vitro." Palaniyar N., Gerasimopoulos E., Evans D.H. J. Mol. Biol. 287:9-20(1999) [PubMed: 10074403] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The complete genome sequence of shope (Rabbit) fibroma virus." Willer D.O., McFadden G., Evans D.H. Virology 264:319-343(1999) [PubMed: 10562495] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M31723 Genomic DNA. Translation: AAA47228.1. AF170722 Genomic DNA. Translation: AAF17956.1.
PIR	ITVZKA. A34769.
RefSeq	NP_051963.1. NC_001266.1.
3D structure databases
HSSP	HSSP built from PDB template 1A41 based on UniProtKB P08585.
ProteinModelPortal	P16472.
SMR	P16472. Positions 1-311.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1486917.
Phylogenomic databases
ProtClustDB	PHA3101.
Family and domain databases
InterPro	IPR011010. DNA_brk_join_enz. IPR018521. TopoI_AS. IPR001631. TopoI_C. IPR014711. TopoI_cat_a-hlx-sub_euk. IPR013500. TopoI_cat_euk. IPR015346. TopoI_N_vir. [Graphical view]
Gene3D	G3DSA:3.30.66.10. G3DSA:3.30.66.10. 1 hit. G3DSA:3.90.15.10. TopoI_cat_a-hlx-sub_euk. 1 hit.
Pfam	PF01028. Topoisom_I. 1 hit. PF09266. VirDNA-topo-I_N. 1 hit. [Graphical view]
PRINTS	PR00416. EUTPISMRASEI.
SUPFAM	SSF56349. DNA_brk_join_enz. 1 hit.
PROSITE	PS00176. TOPOISOMERASE_I_EUK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TOP1_RFVKA

Accession

Primary (citable) accession number: P16472
Secondary accession number(s): Q77PC2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	December 14, 2011
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program