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P16472

- TOP1_RFVKA

UniProt

P16472 - TOP1_RFVKA

Protein

DNA topoisomerase 1

Gene

TOP1

Organism
Rabbit fibroma virus (strain Kasza) (RFV) (Shope fibroma virus (strain Kasza))
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 1 (01 Aug 1990)
      Previous versions | rss
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    Functioni

    Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.By similarity

    Catalytic activityi

    ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei273 – 2731O-(3'-phospho-DNA)-tyrosine intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA topoisomerase type I activity Source: UniProtKB-EC
    3. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: InterPro

    GO - Biological processi

    1. DNA topological change Source: InterPro

    Keywords - Molecular functioni

    Isomerase, Topoisomerase

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA topoisomerase 1 (EC:5.99.1.2)
    Alternative name(s):
    DNA topoisomerase I
    Gene namesi
    Name:TOP1
    ORF Names:s074R
    OrganismiRabbit fibroma virus (strain Kasza) (RFV) (Shope fibroma virus (strain Kasza))
    Taxonomic identifieri10272 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeLeporipoxvirus
    Virus hostiOryctolagus cuniculus (Rabbit) [TaxID: 9986]
    ProteomesiUP000000868: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 314314DNA topoisomerase 1PRO_0000145213Add
    BLAST

    Expressioni

    Keywords - Developmental stagei

    Late protein

    Structurei

    3D structure databases

    ProteinModelPortaliP16472.
    SMRiP16472. Positions 1-311.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the type IB topoisomerase family.Curated

    Family and domain databases

    Gene3Di1.20.120.380. 1 hit.
    3.30.66.10. 1 hit.
    3.90.15.10. 1 hit.
    InterProiIPR011010. DNA_brk_join_enz.
    IPR001631. TopoI.
    IPR018521. TopoI_AS.
    IPR014711. TopoI_cat_a-hlx-sub_euk.
    IPR027362. TopoI_cat_dom2.
    IPR013500. TopoI_cat_euk.
    IPR015346. TopoI_N_vir.
    [Graphical view]
    PfamiPF01028. Topoisom_I. 1 hit.
    PF09266. VirDNA-topo-I_N. 1 hit.
    [Graphical view]
    PRINTSiPR00416. EUTPISMRASEI.
    SUPFAMiSSF56349. SSF56349. 1 hit.
    PROSITEiPS00176. TOPOISOMERASE_I_EUK. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P16472-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRAFTYKDGK LYEDKELTIP VHCSNPTYEI LKHVKIPSHL TDVIVYEQTY    50
    EQSLSRLIFV GLDSKGRRQY FYGKMHVQRR NSARDTIFIK VHRVIDKIHK 100
    FIDDTIEHKN DVLFQLGVFM LMETSFFIRM GKVKYLKEND TVGLLTLKNK 150
    NIVRENRKIL IHFVGKDKII HNFTVHSSNR LYKPLLRLIG RKEPDSFLFH 200
    KLSEKKVYKA VQQFGIRLKD LRTYGVNYTF LYNFWTNVKS LNPIPPIKKM 250
    ISTSIKQTAD IVGHTPSISK RAYIANTVLE YLTHDSELIN TIRDISFDEF 300
    IRLITDYITN TQTV 314
    Length:314
    Mass (Da):36,999
    Last modified:August 1, 1990 - v1
    Checksum:iAF602490AD148161
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M31723 Genomic DNA. Translation: AAA47228.1.
    AF170722 Genomic DNA. Translation: AAF17956.1.
    PIRiA34769. ITVZKA.
    RefSeqiNP_051963.1. NC_001266.1.

    Genome annotation databases

    GeneIDi1486917.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M31723 Genomic DNA. Translation: AAA47228.1 .
    AF170722 Genomic DNA. Translation: AAF17956.1 .
    PIRi A34769. ITVZKA.
    RefSeqi NP_051963.1. NC_001266.1.

    3D structure databases

    ProteinModelPortali P16472.
    SMRi P16472. Positions 1-311.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1486917.

    Family and domain databases

    Gene3Di 1.20.120.380. 1 hit.
    3.30.66.10. 1 hit.
    3.90.15.10. 1 hit.
    InterProi IPR011010. DNA_brk_join_enz.
    IPR001631. TopoI.
    IPR018521. TopoI_AS.
    IPR014711. TopoI_cat_a-hlx-sub_euk.
    IPR027362. TopoI_cat_dom2.
    IPR013500. TopoI_cat_euk.
    IPR015346. TopoI_N_vir.
    [Graphical view ]
    Pfami PF01028. Topoisom_I. 1 hit.
    PF09266. VirDNA-topo-I_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00416. EUTPISMRASEI.
    SUPFAMi SSF56349. SSF56349. 1 hit.
    PROSITEi PS00176. TOPOISOMERASE_I_EUK. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and DNA sequence of the Shope fibroma virus DNA topoisomerase gene."
      Upton C., Opgenorth A., Traktman P., McFadden G.
      Virology 176:439-447(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Sequence and analysis of a portion of the genomes of Shope fibroma virus and malignant rabbit fibroma virus that is important for viral replication in lymphocytes."
      Strayer D.S., Jerng H.H., O'Connor K.
      Virology 185:585-595(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Shope fibroma virus DNA topoisomerase catalyses holliday junction resolution and hairpin formation in vitro."
      Palaniyar N., Gerasimopoulos E., Evans D.H.
      J. Mol. Biol. 287:9-20(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "The complete genome sequence of shope (Rabbit) fibroma virus."
      Willer D.O., McFadden G., Evans D.H.
      Virology 264:319-343(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiTOP1_RFVKA
    AccessioniPrimary (citable) accession number: P16472
    Secondary accession number(s): Q77PC2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: August 1, 1990
    Last modified: October 1, 2014
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3