Reviewed, UniProtKB/Swiss-Prot P16472 (TOP1_RFVKA)

Names and origin

Protein names

Recommended name:
    DNA topoisomerase 1
    EC=5.99.1.2
Alternative name(s):
    DNA topoisomerase I

Gene names

Name:

TOP1

Organism

Rabbit fibroma virus (strain Kasza) (RFV) (Shope fibroma virus (strain Kasza))

Taxonomic identifier

10272 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Leporipoxvirus

Virus host

Oryctolagus cuniculus (Rabbit) [TaxID: 9986]

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
Miscellaneous	When a topoisomerase transiently breaks a DNA backbone bond, it simultaneously forms a protein-DNA link, in which a tyrosyl oxygen in the enzyme is joined to a DNA phosphorus at one end of the enzyme-severed DNA strand.
Sequence similarities	Belongs to the eukaryotic type I topoisomerase family.

Ontologies

Keywords
Developmental stage	Late protein
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro DNA unwinding during replication Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	DNA topoisomerase 1		PRO_0000145213
Sites
	Active site	273	1	O-(3'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P16472-1 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: AF602490AD148161	FASTA	314	36,999
	10 20 30 40 50 60 MRAFTYKDGK LYEDKELTIP VHCSNPTYEI LKHVKIPSHL TDVIVYEQTY EQSLSRLIFV 70 80 90 100 110 120 GLDSKGRRQY FYGKMHVQRR NSARDTIFIK VHRVIDKIHK FIDDTIEHKN DVLFQLGVFM 130 140 150 160 170 180 LMETSFFIRM GKVKYLKEND TVGLLTLKNK NIVRENRKIL IHFVGKDKII HNFTVHSSNR 190 200 210 220 230 240 LYKPLLRLIG RKEPDSFLFH KLSEKKVYKA VQQFGIRLKD LRTYGVNYTF LYNFWTNVKS 250 260 270 280 290 300 LNPIPPIKKM ISTSIKQTAD IVGHTPSISK RAYIANTVLE YLTHDSELIN TIRDISFDEF 310 IRLITDYITN TQTV

References

[1]	"Identification and DNA sequence of the Shope fibroma virus DNA topoisomerase gene." Upton C., Opgenorth A., Traktman P., McFadden G. Virology 176:439-447(1990) [PubMed: 2161144] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M31723 Genomic DNA. Translation: AAA47228.1.
PIR	ITVZKA. A34769.
RefSeq	NP_051963.1.
3D structure databases
SMR	P16472. Positions 1-311.
ModBase	Search...
Genome annotation databases
GeneID	1486917.
Family and domain databases
InterPro	IPR011010. DNA_brk_join_enz. IPR018521. TopoI_AS. IPR001631. TopoI_C. IPR014711. TopoI_cat_a-hlx-sub_euk. IPR015346. TopoI_N_vir. [Graphical view]
Gene3D	G3DSA:3.90.15.10. TopoI_cat_a-hlx-sub_euk. 1 hit.
Pfam	PF09266. VirDNA-topo-I_N. 1 hit. [Graphical view]
PRINTS	PR00416. EUTPISMRASEI.
PROSITE	PS00176. TOPOISOMERASE_I_EUK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TOP1_RFVKA

Accession

Primary (citable) accession number: P16472

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	February 9, 2010
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)