Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA topoisomerase 1

Gene

TOP1

Organism
Rabbit fibroma virus (strain Kasza) (RFV) (Shope fibroma virus (strain Kasza))
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).By similarity

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei273 – 2731O-(3'-phospho-DNA)-tyrosine intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA topoisomerase type I activity Source: UniProtKB-EC
  3. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: InterPro

GO - Biological processi

  1. DNA topological change Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1 (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase I
Gene namesi
Name:TOP1
ORF Names:s074R
OrganismiRabbit fibroma virus (strain Kasza) (RFV) (Shope fibroma virus (strain Kasza))
Taxonomic identifieri10272 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeLeporipoxvirus
Virus hostiOryctolagus cuniculus (Rabbit) [TaxID: 9986]
ProteomesiUP000000868: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 314314DNA topoisomerase 1PRO_0000145213Add
BLAST

Expressioni

Keywords - Developmental stagei

Late protein

Structurei

3D structure databases

ProteinModelPortaliP16472.
SMRiP16472. Positions 1-311.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the type IB topoisomerase family.Curated

Family and domain databases

Gene3Di1.20.120.380. 1 hit.
3.30.66.10. 1 hit.
3.90.15.10. 1 hit.
InterProiIPR011010. DNA_brk_join_enz.
IPR001631. TopoI.
IPR018521. TopoI_AS.
IPR014711. TopoI_cat_a-hlx-sub_euk.
IPR027362. TopoI_cat_dom2.
IPR013500. TopoI_cat_euk.
IPR015346. TopoI_N_vir.
[Graphical view]
PfamiPF01028. Topoisom_I. 1 hit.
PF09266. VirDNA-topo-I_N. 1 hit.
[Graphical view]
PRINTSiPR00416. EUTPISMRASEI.
SUPFAMiSSF56349. SSF56349. 1 hit.
PROSITEiPS00176. TOPOISOMERASE_I_EUK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P16472-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRAFTYKDGK LYEDKELTIP VHCSNPTYEI LKHVKIPSHL TDVIVYEQTY
60 70 80 90 100
EQSLSRLIFV GLDSKGRRQY FYGKMHVQRR NSARDTIFIK VHRVIDKIHK
110 120 130 140 150
FIDDTIEHKN DVLFQLGVFM LMETSFFIRM GKVKYLKEND TVGLLTLKNK
160 170 180 190 200
NIVRENRKIL IHFVGKDKII HNFTVHSSNR LYKPLLRLIG RKEPDSFLFH
210 220 230 240 250
KLSEKKVYKA VQQFGIRLKD LRTYGVNYTF LYNFWTNVKS LNPIPPIKKM
260 270 280 290 300
ISTSIKQTAD IVGHTPSISK RAYIANTVLE YLTHDSELIN TIRDISFDEF
310
IRLITDYITN TQTV
Length:314
Mass (Da):36,999
Last modified:August 1, 1990 - v1
Checksum:iAF602490AD148161
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31723 Genomic DNA. Translation: AAA47228.1.
AF170722 Genomic DNA. Translation: AAF17956.1.
PIRiA34769. ITVZKA.
RefSeqiNP_051963.1. NC_001266.1.

Genome annotation databases

GeneIDi1486917.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31723 Genomic DNA. Translation: AAA47228.1.
AF170722 Genomic DNA. Translation: AAF17956.1.
PIRiA34769. ITVZKA.
RefSeqiNP_051963.1. NC_001266.1.

3D structure databases

ProteinModelPortaliP16472.
SMRiP16472. Positions 1-311.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1486917.

Family and domain databases

Gene3Di1.20.120.380. 1 hit.
3.30.66.10. 1 hit.
3.90.15.10. 1 hit.
InterProiIPR011010. DNA_brk_join_enz.
IPR001631. TopoI.
IPR018521. TopoI_AS.
IPR014711. TopoI_cat_a-hlx-sub_euk.
IPR027362. TopoI_cat_dom2.
IPR013500. TopoI_cat_euk.
IPR015346. TopoI_N_vir.
[Graphical view]
PfamiPF01028. Topoisom_I. 1 hit.
PF09266. VirDNA-topo-I_N. 1 hit.
[Graphical view]
PRINTSiPR00416. EUTPISMRASEI.
SUPFAMiSSF56349. SSF56349. 1 hit.
PROSITEiPS00176. TOPOISOMERASE_I_EUK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and DNA sequence of the Shope fibroma virus DNA topoisomerase gene."
    Upton C., Opgenorth A., Traktman P., McFadden G.
    Virology 176:439-447(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequence and analysis of a portion of the genomes of Shope fibroma virus and malignant rabbit fibroma virus that is important for viral replication in lymphocytes."
    Strayer D.S., Jerng H.H., O'Connor K.
    Virology 185:585-595(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Shope fibroma virus DNA topoisomerase catalyses holliday junction resolution and hairpin formation in vitro."
    Palaniyar N., Gerasimopoulos E., Evans D.H.
    J. Mol. Biol. 287:9-20(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "The complete genome sequence of shope (Rabbit) fibroma virus."
    Willer D.O., McFadden G., Evans D.H.
    Virology 264:319-343(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiTOP1_RFVKA
AccessioniPrimary (citable) accession number: P16472
Secondary accession number(s): Q77PC2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: January 7, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.