Reviewed,
UniProtKB/Swiss-Prot P16471 (PRLR_HUMAN)
Last modified
June 16, 2009.
Version 113.
History...
Clusters with 100%,
90%,
50% identity |
Documents (6) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Prolactin receptor Short name=PRL-R | ||
| Gene names |
| ||
| Organism | Homo sapiens (Human) | ||
| Taxonomic identifier | 9606 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 622 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | This is a receptor for the anterior pituitary hormone prolactin (PRL). Isoforms 4 and 6 are unable to transduce prolactin signaling. Ref.6 |
| Subunit structure | |
| Subcellular location | Membrane; Single-pass type I membrane protein. Secreted. Note: Isoform 7. Ref.6 Ref.4 |
| Tissue specificity | Expressed in breast, placenta, kidney, liver and pancreas. Ref.6 Ref.4 |
| Domain | The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box 1 motif is required for JAK interaction and/or activation. |
| Sequence similarities | Belongs to the type I cytokine receptor family. Type 1 subfamily. Contains 2 fibronectin type-III domains. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| GH1 | P01241 | 1 | EBI-476182,EBI-1026046 | |
| NEK3 | P51956 | 1 | EBI-476182,EBI-476041 | |
| VAV2 | P52735 | 1 | EBI-476182,EBI-297549 |
Alternative products
| This entry describes 8 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: P16471-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: P16471-2) Also known as: Delta-S1; The sequence of this isoform differs from the canonical sequence as follows: 24-124: Missing. | ||||||
| Isoform 3 (identifier: P16471-3) The sequence of this isoform differs from the canonical sequence as follows: 229-230: DF → AW 231-622: Missing. | ||||||
| Note: Soluble isoform that appears specific for the BT-474 breast cancer cell line. | ||||||
| Isoform 4 (identifier: P16471-4) Also known as: SF1a; Short form 1a; The sequence of this isoform differs from the canonical sequence as follows: 338-376: MKPTYLDPDT...PQANPSTFYD → DPLMLGASHY...FTKATLTTVQ 377-622: Missing. | ||||||
| Note: Includes exon 11. Does not transduce prolactin signaling. | ||||||
| Isoform 5 (identifier: P16471-5) Also known as: Intermediate; The sequence of this isoform differs from the canonical sequence as follows: 337-349: GMKPTYLDPDTDS → EREQRQAQEARDS 350-622: Missing. | ||||||
| Note: Produced by deletion of part of exon 10 and frameshift. | ||||||
| Isoform 6 (identifier: P16471-6) Also known as: SF1b; Short form 1b; The sequence of this isoform differs from the canonical sequence as follows: 286-288: KGK → VTP 289-622: Missing. | ||||||
| Note: Does not transduce prolactin signaling. | ||||||
| Isoform 7 (identifier: P16471-7) Also known as: Delta 7/11; The sequence of this isoform differs from the canonical sequence as follows: 229-268: DFTMNDTTVW...KGYSMVTCIF → GDPLMLGASH...FTKATLTTVQ 269-622: Missing. | ||||||
| Note: Splices from exon 7 to exon 11. Secreted. | ||||||
| Isoform 8 (identifier: P16471-8) Also known as: Delta 4-SF1b; The sequence of this isoform differs from the canonical sequence as follows: 1-71: Missing. 286-288: KGK → VTP 289-622: Missing. | ||||||
| Note: SF1b with deletion of exon 4. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | |||||||||||||||||||||||||||||
Molecule processing | ||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 24 | 24 | ||||||||||||||||||||||||||||||||
| Chain | 25 – 622 | 598 | Prolactin receptor | PRO_0000010977 | ||||||||||||||||||||||||||||||
Regions | ||||||||||||||||||||||||||||||||||
| Topological domain | 25 – 234 | 210 | Extracellular Potential | |||||||||||||||||||||||||||||||
| Transmembrane | 235 – 258 | 24 | Potential | |||||||||||||||||||||||||||||||
| Topological domain | 259 – 622 | 364 | Cytoplasmic Potential | |||||||||||||||||||||||||||||||
| Domain | 27 – 121 | 95 | Fibronectin type-III 1 | |||||||||||||||||||||||||||||||
| Domain | 127 – 227 | 101 | Fibronectin type-III 2 | |||||||||||||||||||||||||||||||
| Motif | 215 – 219 | 5 | WSXWS motif | |||||||||||||||||||||||||||||||
| Motif | 267 – 275 | 9 | Box 1 motif | |||||||||||||||||||||||||||||||
Amino acid modifications | ||||||||||||||||||||||||||||||||||
| Glycosylation | 59 | 1 | N-linked (GlcNAc...) Potential | |||||||||||||||||||||||||||||||
| Glycosylation | 104 | 1 | N-linked (GlcNAc...) Potential | |||||||||||||||||||||||||||||||
| Glycosylation | 233 | 1 | N-linked (GlcNAc...) Potential | |||||||||||||||||||||||||||||||
| Disulfide bond | 36 ↔ 46 | Ref.11 | ||||||||||||||||||||||||||||||||
| Disulfide bond | 75 ↔ 86 | Ref.11 | ||||||||||||||||||||||||||||||||
Natural variations | ||||||||||||||||||||||||||||||||||
| Alternative sequence | 1 – 71 | 71 | Missing in isoform 8. | VSP_026531 | ||||||||||||||||||||||||||||||
| Alternative sequence | 24 – 124 | 101 | Missing in isoform 2. | VSP_001720 | ||||||||||||||||||||||||||||||
| Alternative sequence | 229 – 268 | 40 | DFTMN…VTCIF → GDPLMLGASHYKNLKSYRPR KISSQGRLAVFTKATLTTVQ in isoform 7. | VSP_026532 | ||||||||||||||||||||||||||||||
| Alternative sequence | 229 – 230 | 2 | DF → AW in isoform 3. | VSP_012620 | ||||||||||||||||||||||||||||||
| Alternative sequence | 231 – 622 | 392 | Missing in isoform 3. | VSP_012621 | ||||||||||||||||||||||||||||||
| Alternative sequence | 269 – 622 | 354 | Missing in isoform 7. | VSP_026533 | ||||||||||||||||||||||||||||||
| Alternative sequence | 286 – 288 | 3 | KGK → VTP in isoform 6 and isoform 8. | VSP_026534 | ||||||||||||||||||||||||||||||
| Alternative sequence | 289 – 622 | 334 | Missing in isoform 6 and isoform 8. | VSP_026535 | ||||||||||||||||||||||||||||||
| Alternative sequence | 337 – 349 | 13 | GMKPT…PDTDS → EREQRQAQEARDS in isoform 5. | VSP_026536 | ||||||||||||||||||||||||||||||
| Alternative sequence | 338 – 376 | 39 | MKPTY…STFYD → DPLMLGASHYKNLKSYRPRK ISSQGRLAVFTKATLTTVQ in isoform 4. | VSP_026537 | ||||||||||||||||||||||||||||||
| Alternative sequence | 350 – 622 | 273 | Missing in isoform 5. | VSP_026538 | ||||||||||||||||||||||||||||||
| Alternative sequence | 377 – 622 | 246 | Missing in isoform 4. | VSP_026539 | ||||||||||||||||||||||||||||||
| Natural variant | 100 | 1 | I → V: dbSNP rs2228482. | VAR_049172 | ||||||||||||||||||||||||||||||
Secondary structure | ||||||||||||||||||||||||||||||||||
Helix Strand Turn | ||||||||||||||||||||||||||||||||||
| Beta strand | 31 – 42 | 12 | ||||||||||||||||||||||||||||||||
| Beta strand | 44 – 50 | 7 | ||||||||||||||||||||||||||||||||
| Beta strand | 61 – 68 | 8 | ||||||||||||||||||||||||||||||||
| Beta strand | 83 – 88 | 6 | ||||||||||||||||||||||||||||||||
| Beta strand | 98 – 106 | 9 | ||||||||||||||||||||||||||||||||
| Beta strand | 117 – 120 | 4 | ||||||||||||||||||||||||||||||||
| Helix | 121 – 123 | 3 | ||||||||||||||||||||||||||||||||
| Beta strand | 141 – 143 | 3 | ||||||||||||||||||||||||||||||||
| Turn | 159 – 162 | 4 | ||||||||||||||||||||||||||||||||
| Beta strand | 166 – 176 | 11 | ||||||||||||||||||||||||||||||||
| Beta strand | 181 – 186 | 6 | ||||||||||||||||||||||||||||||||
| Beta strand | 189 – 191 | 3 | ||||||||||||||||||||||||||||||||
| Beta strand | 201 – 210 | 10 | ||||||||||||||||||||||||||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Identification of a cDNA encoding a long form of prolactin receptor in human hepatoma and breast cancer cells." Boutin J.-M., Edery M., Shirota M., Jolicoeur C., Lesueur L., Ali S., Gould D., Djiane J., Kelly P.A. Mol. Endocrinol. 3:1455-1461(1989) [PubMed: 2558309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). |
| [2] | "Functional characterization of the intermediate isoform of the human prolactin receptor." Kline J.B., Roehrs H., Clevenger C.V. J. Biol. Chem. 274:35461-35468(1999) [PubMed: 10585417] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5). |
| [3] | "The human prolactin receptor gene structure and alternative promoter utilization: the generic promoter hPIII and a novel human promoter hP(N)." Hu Z.-Z., Zhuang L., Meng J., Leondires M., Dufau M.L. J. Clin. Endocrinol. Metab. 84:1153-1156(1999) [PubMed: 10084611] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [4] | "Isolation and characterization of two novel forms of the human prolactin receptor generated by alternative splicing of a newly identified exon 11." Hu Z.Z., Meng J., Dufau M.L. J. Biol. Chem. 276:41086-41094(2001) [PubMed: 11518703] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 4 AND 6), INTERACTION WITH GH1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. |
| [5] | "Characterization of a novel and functional human prolactin receptor isoform (deltaS1PRLr) containing only one extracellular fibronectin-like domain." Kline J.B., Rycyzyn M.A., Clevenger C.V. Mol. Endocrinol. 16:2310-2322(2002) [PubMed: 12351696] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Mammary carcinoma. |
| [6] | "Alternative splicing to exon 11 of human prolactin receptor gene results in multiple isoforms including a secreted prolactin-binding protein." Trott J.F., Hovey R.C., Koduri S., Vonderhaar B.K. J. Mol. Endocrinol. 30:31-47(2003) [PubMed: 12580759] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 4; 6; 7 AND 8), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. |
| [7] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Placenta. |
| [8] | "Prolactin receptor antagonists that inhibit the growth of breast cancer cell lines." Fuh G., Wells J.A. J. Biol. Chem. 270:13133-13137(1995) [PubMed: 7768908] [Abstract] Cited for: PARTIAL NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3). Tissue: Mammary carcinoma. |
| [9] | "The imitation switch protein SNF2L regulates steroidogenic acute regulatory protein expression during terminal differentiation of ovarian granulosa cells." Lazzaro M.A., Pepin D., Pescador N., Murphy B.D., Vanderhyden B.C., Picketts D.J. Mol. Endocrinol. 20:2406-2417(2006) [PubMed: 16740656] [Abstract] Cited for: INTERACTION WITH SMARCA1. |
| [10] | "The X-ray structure of a growth hormone-prolactin receptor complex." Somers W., Ultsch M., de Vos A.M., Kossiakoff A.A. Nature 372:478-481(1994) [PubMed: 7984244] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF 25-235. |
| [11] | "Crystal structure of a prolactin receptor antagonist bound to the extracellular domain of the prolactin receptor." Svensson L.A., Bondensgaard K., Noerskov-Lauritsen L., Christensen L., Becker P., Andersen M.D., Maltesen M.J., Rand K.D., Breinholt J. J. Biol. Chem. 283:19085-19094(2008) [PubMed: 18467331] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 25-234 IN COMPLEX WITH PRL, DISULFIDE BONDS. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| M31661 mRNA. Translation: AAA60174.1. AF166329 mRNA. Translation: AAD49855.1. AF091870 AF091869 Genomic DNA. Translation: AAD32032.1. AF349939 mRNA. Translation: AAK32703.1. AF416618 mRNA. Translation: AAL23914.1. AF416619 mRNA. Translation: AAL23915.1. AF493069 mRNA. Translation: AAM11661.1. AF492470 mRNA. Translation: AAM18048.1. BC059392 mRNA. Translation: AAH59392.1. S78505 mRNA. Translation: AAB34470.1. | |||||||||||||||||||
| IPI | IPI00028640. IPI00044214. IPI00152476. IPI00163730. IPI00219095. IPI00852725. IPI00853144. IPI00853288. | ||||||||||||||||||
| PIR | A40144. A59405. B59405. | ||||||||||||||||||
| RefSeq | NP_000940.1. | ||||||||||||||||||
| UniGene | Hs.368587 | ||||||||||||||||||
3D structure databases | |||||||||||||||||||
| |||||||||||||||||||
| ModBase | Search... | ||||||||||||||||||
Protein-protein interaction databases | |||||||||||||||||||
| DIP | DIP:288N. | ||||||||||||||||||
| IntAct | P16471. 3 interactions. | ||||||||||||||||||
PTM databases | |||||||||||||||||||
| PhosphoSite | P16471. | ||||||||||||||||||
Proteomic databases | |||||||||||||||||||
| PRIDE | P16471. | ||||||||||||||||||
Genome annotation databases | |||||||||||||||||||
| Ensembl | ENSG00000113494. Homo sapiens. [Contig view] | ||||||||||||||||||
| GeneID | 5618. | ||||||||||||||||||
| KEGG | hsa:5618. | ||||||||||||||||||
Organism-specific databases | |||||||||||||||||||
| GeneCards | GC05M035084. | ||||||||||||||||||
| H-InvDB | HIX0004802. | ||||||||||||||||||
| HGNC | HGNC:9446. PRLR. | ||||||||||||||||||
| MIM | 176761. gene. | ||||||||||||||||||
| Orphanet | 50920. Mammary polyadenomatosis. | ||||||||||||||||||
| PharmGKB | PA33791. | ||||||||||||||||||
| GenAtlas | Search... | ||||||||||||||||||
Phylogenomic databases | |||||||||||||||||||
| HOGENOM | P16471. | ||||||||||||||||||
| HOVERGEN | P16471. | ||||||||||||||||||
| OMA | P16471. YTEEARE. | ||||||||||||||||||
Enzyme and pathway databases | |||||||||||||||||||
| Pathway_Interaction_DB | ptp1bpathway. Signaling events mediated by PTP1B. | ||||||||||||||||||
Gene expression databases | |||||||||||||||||||
| ArrayExpress | P16471. | ||||||||||||||||||
| Bgee | P16471. | ||||||||||||||||||
| CleanEx | HS_PRLR. | ||||||||||||||||||
| GermOnline | ENSG00000113494. Homo sapiens. | ||||||||||||||||||
Family and domain databases | |||||||||||||||||||
| InterPro | IPR008957. Fibronectin_typ-III-like_fold. IPR003961. FN_III. IPR003528. Long_hematopoietin_rcpt_CS. [Graphical view] | ||||||||||||||||||
| Gene3D | G3DSA:2.60.40.30. FN_III-like. 2 hits. | ||||||||||||||||||
| Pfam | PF00041. fn3. 2 hits. [Graphical view] | ||||||||||||||||||
| SMART | SM00060. FN3. 2 hits. [Graphical view] | ||||||||||||||||||
| PROSITE | PS50853. FN3. 2 hits. PS01352. HEMATOPO_REC_L_F1. 1 hit. [Graphical view] | ||||||||||||||||||
| ProtoNet | Search... | ||||||||||||||||||
Other Resources | |||||||||||||||||||
| DrugBank | DB00858. Dromostanolone. DB01185. Fluoxymesterone. DB00082. Pegvisomant. DB00052. Somatropin recombinant. | ||||||||||||||||||
| NextBio | 21832. | ||||||||||||||||||
| SOURCE | Search... | ||||||||||||||||||
Entry information
| Entry name | PRLR_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P16471 Secondary accession number(s): Q16354 Q9UHJ5 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 5 Human chromosome 5: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with


