Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P16460

- ASSY_MOUSE

UniProt

P16460 - ASSY_MOUSE

Protein

Argininosuccinate synthase

Gene

Ass1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 1 (01 Aug 1990)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Is indirectly involved in the control of blood pressure.By similarity

    Catalytic activityi

    ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei36 – 361ATP; via amide nitrogen and carbonyl oxygenBy similarity
    Binding sitei87 – 871CitrullineBy similarity
    Binding sitei92 – 921CitrullineBy similarity
    Binding sitei119 – 1191AspartateBy similarity
    Binding sitei123 – 1231AspartateBy similarity
    Binding sitei123 – 1231CitrullineBy similarity
    Binding sitei124 – 1241AspartateBy similarity
    Binding sitei127 – 1271CitrullineBy similarity
    Binding sitei180 – 1801CitrullineBy similarity
    Binding sitei189 – 1891CitrullineBy similarity
    Binding sitei270 – 2701CitrullineBy similarity
    Binding sitei282 – 2821CitrullineBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 189ATPBy similarity
    Nucleotide bindingi115 – 1239ATPBy similarity

    GO - Molecular functioni

    1. argininosuccinate synthase activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. arginine biosynthetic process Source: UniProtKB-UniPathway
    2. urea cycle Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Amino-acid biosynthesis, Arginine biosynthesis, Urea cycle

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00068; UER00113.
    UPA00158; UER00272.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Argininosuccinate synthase (EC:6.3.4.5)
    Alternative name(s):
    Citrulline--aspartate ligase
    Gene namesi
    Name:Ass1
    Synonyms:Ass
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:88090. Ass1.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrion Source: MGI

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 412412Argininosuccinate synthasePRO_0000148555Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei180 – 1801PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP16460.
    PaxDbiP16460.
    PRIDEiP16460.

    2D gel databases

    REPRODUCTION-2DPAGEP16460.

    PTM databases

    PhosphoSiteiP16460.

    Expressioni

    Gene expression databases

    BgeeiP16460.
    CleanExiMM_ASS1.
    GenevestigatoriP16460.

    Interactioni

    Subunit structurei

    Homotetramer. Interacts with NMRAL1 By similarity.By similarity

    Protein-protein interaction databases

    IntActiP16460. 5 interactions.
    MINTiMINT-1856903.

    Structurei

    3D structure databases

    ProteinModelPortaliP16460.
    SMRiP16460. Positions 4-407.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0137.
    HOGENOMiHOG000230093.
    HOVERGENiHBG001717.
    InParanoidiP16460.
    KOiK01940.
    OMAiAPPEEAY.
    OrthoDBiEOG7PVWPB.
    PhylomeDBiP16460.
    TreeFamiTF300736.

    Family and domain databases

    Gene3Di3.40.50.620. 1 hit.
    3.90.1260.10. 1 hit.
    HAMAPiMF_00005. Arg_succ_synth_type1.
    InterProiIPR001518. Arginosuc_synth.
    IPR018223. Arginosuc_synth_CS.
    IPR023434. Arginosuc_synth_type_1_subfam.
    IPR024074. AS_cat/multimer_dom_body.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PfamiPF00764. Arginosuc_synth. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00032. argG. 1 hit.
    PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
    PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P16460-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSKGSVVLA YSGGLDTSCI LVWLKEQGYD VIAYLANIGQ KEDFEEARKK    50
    ALKLGAKKVF IEDVSKEFVE EFIWPAVQSS ALYEDRYLLG TSLARPCIAR 100
    RQVEIAQREG AKYVSHGATG KGNDQVRFEL TCYSLAPQIK VIAPWRMPEF 150
    YNRFKGRNDL MEYAKQHGIP IPVTPKSPWS MDENLMHISY EAGILENPKN 200
    QAPPGLYTKT QDPAKAPNSP DVLEIEFKKG VPVKVTNIKD GTTRTTSLEL 250
    FMYLNEVAGK HGVGRIDIVE NRFIGMKSRG IYETPAGTIL YHAHLDIEAF 300
    TMDREVRKIK QGLGLKFAEL VYTGFWHSPE CEFVRHCIQK SQERVEGKVQ 350
    VSVFKGQVYI LGRESPLSLY NEELVSMNVQ GDYEPIDATG FININSLRLK 400
    EYHRLQSKVT AK 412
    Length:412
    Mass (Da):46,584
    Last modified:August 1, 1990 - v1
    Checksum:iA8F3AFDDFBDAEF6A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M31690 mRNA. Translation: AAA37266.1.
    M31692 Genomic DNA. Translation: AAB60707.1.
    M31694, M31693, M31695 Genomic DNA. Translation: AAB60708.1.
    M31702 Genomic DNA. Translation: AAB60706.1.
    BC002074 mRNA. Translation: AAH02074.1.
    BC087556 mRNA. Translation: AAH87556.1.
    CCDSiCCDS15898.1.
    PIRiJU0463. AJMSRS.
    RefSeqiNP_031520.1. NM_007494.3.
    UniGeneiMm.3217.

    Genome annotation databases

    EnsembliENSMUST00000102840; ENSMUSP00000099904; ENSMUSG00000076441.
    GeneIDi11898.
    KEGGimmu:11898.
    UCSCiuc008jdu.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M31690 mRNA. Translation: AAA37266.1 .
    M31692 Genomic DNA. Translation: AAB60707.1 .
    M31694 , M31693 , M31695 Genomic DNA. Translation: AAB60708.1 .
    M31702 Genomic DNA. Translation: AAB60706.1 .
    BC002074 mRNA. Translation: AAH02074.1 .
    BC087556 mRNA. Translation: AAH87556.1 .
    CCDSi CCDS15898.1.
    PIRi JU0463. AJMSRS.
    RefSeqi NP_031520.1. NM_007494.3.
    UniGenei Mm.3217.

    3D structure databases

    ProteinModelPortali P16460.
    SMRi P16460. Positions 4-407.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P16460. 5 interactions.
    MINTi MINT-1856903.

    PTM databases

    PhosphoSitei P16460.

    2D gel databases

    REPRODUCTION-2DPAGE P16460.

    Proteomic databases

    MaxQBi P16460.
    PaxDbi P16460.
    PRIDEi P16460.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000102840 ; ENSMUSP00000099904 ; ENSMUSG00000076441 .
    GeneIDi 11898.
    KEGGi mmu:11898.
    UCSCi uc008jdu.1. mouse.

    Organism-specific databases

    CTDi 445.
    MGIi MGI:88090. Ass1.

    Phylogenomic databases

    eggNOGi COG0137.
    HOGENOMi HOG000230093.
    HOVERGENi HBG001717.
    InParanoidi P16460.
    KOi K01940.
    OMAi APPEEAY.
    OrthoDBi EOG7PVWPB.
    PhylomeDBi P16460.
    TreeFami TF300736.

    Enzyme and pathway databases

    UniPathwayi UPA00068 ; UER00113 .
    UPA00158 ; UER00272 .

    Miscellaneous databases

    NextBioi 279943.
    PROi P16460.
    SOURCEi Search...

    Gene expression databases

    Bgeei P16460.
    CleanExi MM_ASS1.
    Genevestigatori P16460.

    Family and domain databases

    Gene3Di 3.40.50.620. 1 hit.
    3.90.1260.10. 1 hit.
    HAMAPi MF_00005. Arg_succ_synth_type1.
    InterProi IPR001518. Arginosuc_synth.
    IPR018223. Arginosuc_synth_CS.
    IPR023434. Arginosuc_synth_type_1_subfam.
    IPR024074. AS_cat/multimer_dom_body.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    Pfami PF00764. Arginosuc_synth. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00032. argG. 1 hit.
    PROSITEi PS00564. ARGININOSUCCIN_SYN_1. 1 hit.
    PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of the murine argininosuccinate synthetase locus."
      Surh L.C., Beaudet A.L., O'Brien W.E.
      Gene 99:181-189(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
      Strain: DBA/2J.
      Tissue: Liver.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Czech II and FVB/N.
      Tissue: Colon and Mammary gland.
    3. Lubec G., Yang J.W., Zigmond M.
      Submitted (JUL-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 128-140.
      Tissue: Brain.

    Entry informationi

    Entry nameiASSY_MOUSE
    AccessioniPrimary (citable) accession number: P16460
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: August 1, 1990
    Last modified: October 1, 2014
    This is version 127 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3