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Protein

Argininosuccinate synthase

Gene

Ass1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Is indirectly involved in the control of blood pressure.By similarity

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei36 – 361ATP; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei87 – 871CitrullineBy similarity
Binding sitei92 – 921CitrullineBy similarity
Binding sitei119 – 1191AspartateBy similarity
Binding sitei123 – 1231AspartateBy similarity
Binding sitei123 – 1231CitrullineBy similarity
Binding sitei124 – 1241AspartateBy similarity
Binding sitei127 – 1271CitrullineBy similarity
Binding sitei180 – 1801CitrullineBy similarity
Binding sitei189 – 1891CitrullineBy similarity
Binding sitei270 – 2701CitrullineBy similarity
Binding sitei282 – 2821CitrullineBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 189ATPBy similarity
Nucleotide bindingi115 – 1239ATPBy similarity

GO - Molecular functioni

  1. amino acid binding Source: MGI
  2. argininosuccinate synthase activity Source: MGI
  3. ATP binding Source: UniProtKB-KW
  4. poly(A) RNA binding Source: MGI

GO - Biological processi

  1. arginine biosynthetic process Source: GO_Central
  2. argininosuccinate metabolic process Source: MGI
  3. aspartate metabolic process Source: MGI
  4. cellular response to laminar fluid shear stress Source: MGI
  5. citrulline metabolic process Source: MGI
  6. negative regulation of leukocyte cell-cell adhesion Source: MGI
  7. positive regulation of nitric oxide biosynthetic process Source: MGI
  8. urea cycle Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis, Urea cycle

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_246679. Urea cycle.
UniPathwayiUPA00068; UER00113.
UPA00158; UER00272.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthase (EC:6.3.4.5)
Alternative name(s):
Citrulline--aspartate ligase
Gene namesi
Name:Ass1
Synonyms:Ass
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:88090. Ass1.

Subcellular locationi

GO - Cellular componenti

  1. extracellular vesicular exosome Source: MGI
  2. mitochondrion Source: MGI
  3. myelin sheath Source: UniProtKB
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 412412Argininosuccinate synthasePRO_0000148555Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei113 – 1131PhosphotyrosineBy similarity
Modified residuei180 – 1801PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP16460.
PaxDbiP16460.
PRIDEiP16460.

2D gel databases

REPRODUCTION-2DPAGEP16460.

PTM databases

PhosphoSiteiP16460.

Expressioni

Gene expression databases

BgeeiP16460.
CleanExiMM_ASS1.
ExpressionAtlasiP16460. baseline and differential.
GenevestigatoriP16460.

Interactioni

Subunit structurei

Homotetramer. Interacts with NMRAL1 (By similarity).By similarity

Protein-protein interaction databases

IntActiP16460. 5 interactions.
MINTiMINT-1856903.

Structurei

3D structure databases

ProteinModelPortaliP16460.
SMRiP16460. Positions 4-407.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
HOVERGENiHBG001717.
InParanoidiP16460.
KOiK01940.
OMAiLTKWQLY.
OrthoDBiEOG7PVWPB.
PhylomeDBiP16460.
TreeFamiTF300736.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P16460-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSKGSVVLA YSGGLDTSCI LVWLKEQGYD VIAYLANIGQ KEDFEEARKK
60 70 80 90 100
ALKLGAKKVF IEDVSKEFVE EFIWPAVQSS ALYEDRYLLG TSLARPCIAR
110 120 130 140 150
RQVEIAQREG AKYVSHGATG KGNDQVRFEL TCYSLAPQIK VIAPWRMPEF
160 170 180 190 200
YNRFKGRNDL MEYAKQHGIP IPVTPKSPWS MDENLMHISY EAGILENPKN
210 220 230 240 250
QAPPGLYTKT QDPAKAPNSP DVLEIEFKKG VPVKVTNIKD GTTRTTSLEL
260 270 280 290 300
FMYLNEVAGK HGVGRIDIVE NRFIGMKSRG IYETPAGTIL YHAHLDIEAF
310 320 330 340 350
TMDREVRKIK QGLGLKFAEL VYTGFWHSPE CEFVRHCIQK SQERVEGKVQ
360 370 380 390 400
VSVFKGQVYI LGRESPLSLY NEELVSMNVQ GDYEPIDATG FININSLRLK
410
EYHRLQSKVT AK
Length:412
Mass (Da):46,584
Last modified:August 1, 1990 - v1
Checksum:iA8F3AFDDFBDAEF6A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31690 mRNA. Translation: AAA37266.1.
M31692 Genomic DNA. Translation: AAB60707.1.
M31694, M31693, M31695 Genomic DNA. Translation: AAB60708.1.
M31702 Genomic DNA. Translation: AAB60706.1.
BC002074 mRNA. Translation: AAH02074.1.
BC087556 mRNA. Translation: AAH87556.1.
CCDSiCCDS15898.1.
PIRiJU0463. AJMSRS.
RefSeqiNP_031520.1. NM_007494.3.
UniGeneiMm.3217.

Genome annotation databases

EnsembliENSMUST00000102840; ENSMUSP00000099904; ENSMUSG00000076441.
GeneIDi11898.
KEGGimmu:11898.
UCSCiuc008jdu.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31690 mRNA. Translation: AAA37266.1.
M31692 Genomic DNA. Translation: AAB60707.1.
M31694, M31693, M31695 Genomic DNA. Translation: AAB60708.1.
M31702 Genomic DNA. Translation: AAB60706.1.
BC002074 mRNA. Translation: AAH02074.1.
BC087556 mRNA. Translation: AAH87556.1.
CCDSiCCDS15898.1.
PIRiJU0463. AJMSRS.
RefSeqiNP_031520.1. NM_007494.3.
UniGeneiMm.3217.

3D structure databases

ProteinModelPortaliP16460.
SMRiP16460. Positions 4-407.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP16460. 5 interactions.
MINTiMINT-1856903.

PTM databases

PhosphoSiteiP16460.

2D gel databases

REPRODUCTION-2DPAGEP16460.

Proteomic databases

MaxQBiP16460.
PaxDbiP16460.
PRIDEiP16460.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102840; ENSMUSP00000099904; ENSMUSG00000076441.
GeneIDi11898.
KEGGimmu:11898.
UCSCiuc008jdu.1. mouse.

Organism-specific databases

CTDi445.
MGIiMGI:88090. Ass1.

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
HOVERGENiHBG001717.
InParanoidiP16460.
KOiK01940.
OMAiLTKWQLY.
OrthoDBiEOG7PVWPB.
PhylomeDBiP16460.
TreeFamiTF300736.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.
UPA00158; UER00272.
ReactomeiREACT_246679. Urea cycle.

Miscellaneous databases

NextBioi279943.
PROiP16460.
SOURCEiSearch...

Gene expression databases

BgeeiP16460.
CleanExiMM_ASS1.
ExpressionAtlasiP16460. baseline and differential.
GenevestigatoriP16460.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterization of the murine argininosuccinate synthetase locus."
    Surh L.C., Beaudet A.L., O'Brien W.E.
    Gene 99:181-189(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: DBA/2J.
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II and FVB/N.
    Tissue: Colon and Mammary gland.
  3. Lubec G., Yang J.W., Zigmond M.
    Submitted (JUL-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 128-140.
    Tissue: Brain.

Entry informationi

Entry nameiASSY_MOUSE
AccessioniPrimary (citable) accession number: P16460
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: March 4, 2015
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.