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P16460 (ASSY_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Argininosuccinate synthase

EC=6.3.4.5
Alternative name(s):
Citrulline--aspartate ligase
Gene names
Name:Ass1
Synonyms:Ass
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length412 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Is indirectly involved in the control of blood pressure By similarity. HAMAP-Rule MF_00005

Catalytic activity

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP-Rule MF_00005

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP-Rule MF_00005

Nitrogen metabolism; urea cycle; (N(omega)-L-arginino)succinate from L-aspartate and L-citrulline: step 1/1. HAMAP-Rule MF_00005

Subunit structure

Homotetramer. Interacts with NMRAL1 By similarity. HAMAP-Rule MF_00005

Sequence similarities

Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 412412Argininosuccinate synthase HAMAP-Rule MF_00005
PRO_0000148555

Regions

Nucleotide binding10 – 189ATP By similarity
Nucleotide binding115 – 1239ATP By similarity

Sites

Binding site361ATP; via amide nitrogen and carbonyl oxygen By similarity
Binding site871Citrulline By similarity
Binding site921Citrulline By similarity
Binding site1191Aspartate By similarity
Binding site1231Aspartate By similarity
Binding site1231Citrulline By similarity
Binding site1241Aspartate By similarity
Binding site1271Citrulline By similarity
Binding site1801Citrulline By similarity
Binding site1891Citrulline By similarity
Binding site2701Citrulline By similarity
Binding site2821Citrulline By similarity

Amino acid modifications

Modified residue1801Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
P16460 [UniParc].

Last modified August 1, 1990. Version 1.
Checksum: A8F3AFDDFBDAEF6A

FASTA41246,584
        10         20         30         40         50         60 
MSSKGSVVLA YSGGLDTSCI LVWLKEQGYD VIAYLANIGQ KEDFEEARKK ALKLGAKKVF 

        70         80         90        100        110        120 
IEDVSKEFVE EFIWPAVQSS ALYEDRYLLG TSLARPCIAR RQVEIAQREG AKYVSHGATG 

       130        140        150        160        170        180 
KGNDQVRFEL TCYSLAPQIK VIAPWRMPEF YNRFKGRNDL MEYAKQHGIP IPVTPKSPWS 

       190        200        210        220        230        240 
MDENLMHISY EAGILENPKN QAPPGLYTKT QDPAKAPNSP DVLEIEFKKG VPVKVTNIKD 

       250        260        270        280        290        300 
GTTRTTSLEL FMYLNEVAGK HGVGRIDIVE NRFIGMKSRG IYETPAGTIL YHAHLDIEAF 

       310        320        330        340        350        360 
TMDREVRKIK QGLGLKFAEL VYTGFWHSPE CEFVRHCIQK SQERVEGKVQ VSVFKGQVYI 

       370        380        390        400        410 
LGRESPLSLY NEELVSMNVQ GDYEPIDATG FININSLRLK EYHRLQSKVT AK 

« Hide

References

« Hide 'large scale' references
[1]"Molecular characterization of the murine argininosuccinate synthetase locus."
Surh L.C., Beaudet A.L., O'Brien W.E.
Gene 99:181-189(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Strain: DBA/2J.
Tissue: Liver.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Czech II and FVB/N.
Tissue: Colon and Mammary gland.
[3]Lubec G., Yang J.W., Zigmond M.
Submitted (JUL-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 128-140.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M31690 mRNA. Translation: AAA37266.1.
M31692 Genomic DNA. Translation: AAB60707.1.
M31694, M31693, M31695 Genomic DNA. Translation: AAB60708.1.
M31702 Genomic DNA. Translation: AAB60706.1.
BC002074 mRNA. Translation: AAH02074.1.
BC087556 mRNA. Translation: AAH87556.1.
PIRAJMSRS. JU0463.
RefSeqNP_031520.1. NM_007494.3.
UniGeneMm.3217.

3D structure databases

ProteinModelPortalP16460.
SMRP16460. Positions 4-407.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP16460. 5 interactions.
MINTMINT-1856903.

PTM databases

PhosphoSiteP16460.

2D gel databases

REPRODUCTION-2DPAGEP16460.

Proteomic databases

PaxDbP16460.
PRIDEP16460.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000102840; ENSMUSP00000099904; ENSMUSG00000076441.
GeneID11898.
KEGGmmu:11898.
UCSCuc008jdu.1. mouse.

Organism-specific databases

CTD445.
MGIMGI:88090. Ass1.

Phylogenomic databases

eggNOGCOG0137.
HOGENOMHOG000230093.
HOVERGENHBG001717.
InParanoidP16460.
KOK01940.
OMAGIKSKFD.
OrthoDBEOG7PVWPB.
PhylomeDBP16460.
TreeFamTF300736.

Enzyme and pathway databases

UniPathwayUPA00068; UER00113.
UPA00158; UER00272.

Gene expression databases

BgeeP16460.
CleanExMM_ASS1.
GenevestigatorP16460.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPMF_00005. Arg_succ_synth_type1.
InterProIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsTIGR00032. argG. 1 hit.
PROSITEPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio279943.
PROP16460.
SOURCESearch...

Entry information

Entry nameASSY_MOUSE
AccessionPrimary (citable) accession number: P16460
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: April 16, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot