P16456 (SELD_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Selenide, water dikinase
EC=2.7.9.3

Alternative name(s):
Selenium donor protein
Selenophosphate synthase

Gene names

Name:	selD
Synonyms:	fdhB
Ordered Locus Names:	b1764, JW1753

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Synthesizes selenophosphate from selenide and ATP. HAMAP MF_00625
Catalytic activity	ATP + selenide + H2O = AMP + selenophosphate + phosphate. HAMAP MF_00625
Cofactor	Magnesium.
Subunit structure	Monomer Potential.
Sequence similarities	Belongs to the selenophosphate synthase 1 family. Class I subfamily.

Ontologies

Keywords
Ligand	ATP-binding Magnesium Nucleotide-binding Selenium
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW selenide, water dikinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 347	347	Selenide, water dikinase HAMAP MF_00625		PRO_0000127620
Regions
	Nucleotide binding	230 – 236	7	ATP Potential
Sites
	Active site	17	1	Potential
	Site	20	1	Important for catalytic activity
Experimental info
	Mutagenesis	13	1	H → N: No loss of activity.
	Mutagenesis	17	1	C → S: Complete loss of activity. Ref.6
	Mutagenesis	18	1	G → V: Partial loss of activity.
	Mutagenesis	19	1	C → S: No loss of activity. Ref.6
	Mutagenesis	20	1	K → Q: Complete loss of activity.
	Mutagenesis	20	1	K → R: Marked loss of activity.

Sequences

	Sequence		Length	Mass (Da)
	P16456 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: 595E5EC9F7B1370F	FASTA	347	36,687
	10 20 30 40 50 60 MSENSIRLTQ YSHGAGCGCK ISPKVLETIL HSEQAKFVDP NLLVGNETRD DAAVYDLGNG 70 80 90 100 110 120 TSVISTTDFF MPIVDNPFDF GRIAATNAIS DIFAMGGKPI MAIAILGWPI NKLSPEIARE 130 140 150 160 170 180 VTEGGRYACR QAGIALAGGH SIDAPEPIFG LAVTGIVPTE RVKKNSTAQA GCKLFLTKPL 190 200 210 220 230 240 GIGVLTTAEK KSLLKPEHQG LATEVMCRMN IAGASFANIE GVKAMTDVTG FGLLGHLSEM 250 260 270 280 290 300 CQGAGVQARV DYEAIPKLPG VEEYIKLGAV PGGTERNFAS YGHLMGEMPR EVRDLLCDPQ 310 320 330 340 TSGGLLLAVM PEAENEVKAT AAEFGIELTA IGELVPARGG RAMVEIR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"In vitro synthesis of selenocysteinyl-tRNA(UCA) from seryl-tRNA(UCA): involvement and characterization of the selD gene product." Leinfelder W., Forchhammer K., Veprek B., Zehelein E., Boeck A. Proc. Natl. Acad. Sci. U.S.A. 87:543-547(1990) [PubMed: 2405383] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[2]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:363-377(1996) [PubMed: 9097039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Molecular cloning and DNA sequence analysis of Escherichia coli topB, the gene encoding topoisomerase III." Digate R.J., Marians K.J. J. Biol. Chem. 264:17924-17930(1989) [PubMed: 2553698] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 236-347. Strain: HMS-83.
[6]	"Escherichia coli mutant SELD enzymes. The cysteine 17 residue is essential for selenophosphate formation from ATP and selenide." Kim I.Y., Veres Z., Stadtman T.C. J. Biol. Chem. 267:19650-19654(1992) [PubMed: 1527085] [Abstract] Cited for: MUTAGENESIS OF CYS-17 AND CYS-19.
[7]	"Biochemical analysis of Escherichia coli selenophosphate synthetase mutants. Lysine 20 is essential for catalytic activity and cysteine 17/19 for 8-azido-ATP derivatization." Kim I.Y., Veres Z., Stadtman T.C. J. Biol. Chem. 268:27020-27025(1993) [PubMed: 8262938] [Abstract] Cited for: MUTAGENESIS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M30184 Genomic DNA. Translation: AAA74748.1. U00096 Genomic DNA. Translation: AAC74834.1. AP009048 Genomic DNA. Translation: BAA15552.1. J05076 Genomic DNA. Translation: AAA83922.1.
PIR	JW0033.
RefSeq	NP_416278.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P16456.
SMR	P16456. Positions 4-342.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-10849N.
IntAct	P16456. 11 interactions.
MINT	MINT-1256873.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000001837; EBESCP00000001837; EBESCG00000001515. EBESCT00000017507; EBESCP00000016798; EBESCG00000016563.
GeneID	946768.
GenomeReviews	Gene locus JW1753 in contig AP009048_GR. Gene locus b1764 in contig U00096_GR.
KEGG	ecj:JW1753. eco:b1764.
PATRIC	32118839. VBIEscCol129921_1837.
Organism-specific databases
EchoBASE	EB0936.
EcoGene	EG10943. selD.
Phylogenomic databases
eggNOG	COG0709.
GeneTree	EBGT00050000011423.
HOGENOM	HBG383791.
OMA	DYIAQGC.
PhylomeDB	P16456.
ProtClustDB	PRK00943.
Enzyme and pathway databases
BioCyc	EcoCyc:EG10943-MONOMER. MetaCyc:EG10943-MONOMER.
Gene expression databases
Genevestigator	P16456.
Family and domain databases
HAMAP	MF_00625. SelD. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR016188. PurM_N-like. IPR004536. SelD. IPR023061. SelD_I. [Graphical view]
KO	K01008.
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. [Graphical view]
PIRSF	PIRSF036407. Selenphspht_syn. 1 hit.
SUPFAM	SSF56042. AIR_synth_C. 1 hit. SSF55326. PurM_N-like. 1 hit.
TIGRFAMs	TIGR00476. SelD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SELD_ECOLI

Accession

Primary (citable) accession number: P16456
Secondary accession number(s): P78172

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	January 25, 2012
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program