P16451 (ODPX_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pyruvate dehydrogenase complex protein X component, mitochondrial

Alternative name(s):
Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex
E3-binding protein
Pyruvate dehydrogenase complex component E3BP

Gene names

Name:	PDX1
Ordered Locus Names:	YGR193C
ORF Names:	G7579

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	410 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex.
Subunit structure	Eukaryotic pyruvate dehydrogenase (PDH) complexes are organized as a core consisting of the oligomeric dihydrolipoamide acetyl-transferase (E2), around which are arranged multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide dehydrogenase (E3) and protein X (E3BP) bound by non-covalent bonds.
Subcellular location	Mitochondrion matrix.
Miscellaneous	Present with 414 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the 2-oxoacid dehydrogenase family. Contains 1 lipoyl-binding domain.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Lipoyl Transit peptide
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	acetyl-CoA biosynthetic process from pyruvate Inferred from direct assay PubMed 7947791. Source: SGD
Cellular_component	mitochondrial pyruvate dehydrogenase complex Inferred from direct assay Ref.6PubMed 7947791. Source: SGD
Molecular_function	structural molecule activity Inferred from direct assay Ref.6. Source: SGD transferase activity, transferring acyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 30	30	Mitochondrion Ref.1
	Chain	31 – 410	380	Pyruvate dehydrogenase complex protein X component, mitochondrial		PRO_0000020487
Regions
	Domain	33 – 107	75	Lipoyl-binding
Amino acid modifications
	Modified residue	73	1	N6-lipoyllysine By similarity
Experimental info
	Sequence conflict	378	1	I → M in AAT93002. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P16451 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: 11649CA28C420CDF	FASTA	410	45,362
	10 20 30 40 50 60 MLSAISKVST LKSCTRYLTK CNYHASAKLL AVKTFSMPAM SPTMEKGGIV SWKYKVGEPF 70 80 90 100 110 120 SAGDVILEVE TDKSQIDVEA LDDGKLAKIL KDEGSKDVDV GEPIAYIADV DDDLATIKLP 130 140 150 160 170 180 QEANTANAKS IEIKKPSADS TEATQQHLKK ATVTPIKTVD GSQANLEQTL LPSVSLLLAE 190 200 210 220 230 240 NNISKQKALK EIAPSGSNGR LLKGDVLAYL GKIPQDSVNK VTEFIKKNER LDLSNIKPIQ 250 260 270 280 290 300 LKPKIAEQAQ TKAADKPKIT PVEFEEQLVF HAPASIPFDK LSESLNSFMK EAYQFSHGTP 310 320 330 340 350 360 LMDTNSKYFD PIFEDLVTLS PREPRFKFSY DLMQIPKANN MQDTYGQEDI FDLLTGSDAT 370 380 390 400 410 ASSVRPVEKN LPEKNEYILA LNVSVNNKKF NDAEAKAKRF LDYVRELESF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and nucleotide sequence of the gene for protein X from Saccharomyces cerevisiae." Behal R.H., Browning K.S., Hall T.B., Reed L.J. Proc. Natl. Acad. Sci. U.S.A. 86:8732-8736(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 31-60 AND 192-206.
[2]	"The complete sequence of a 9037 bp DNA fragment of the right arm of Saccharomyces cerevisiae chromosome VII." Arroyo J., Garcia-Gonzalez M., Garcia-Saez M.I., Sanchez M., Nombela C. Yeast 11:587-591(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"Disruption and mutagenesis of the Saccharomyces cerevisiae PDX1 gene encoding the protein X component of the pyruvate dehydrogenase complex." Lawson J.E., Behal R.H., Reed L.J. Biochemistry 30:2834-2839(1991) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M28222 Genomic DNA. Translation: AAA34910.1. X82408 Genomic DNA. Translation: CAA57804.1. Z72978 Genomic DNA. Translation: CAA97219.1. AY692983 Genomic DNA. Translation: AAT93002.1. BK006941 Genomic DNA. Translation: DAA08286.1.
PIR	DEBYPX. A36183.
RefSeq	NP_011709.1. NM_001181322.1.
3D structure databases
ProteinModelPortal	P16451.
SMR	P16451. Positions 37-120, 169-211.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5550N.
IntAct	P16451. 9 interactions.
MINT	MINT-508924.
STRING	4932.YGR193C.
Proteomic databases
PaxDb	P16451.
PeptideAtlas	P16451.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGR193C; YGR193C; YGR193C.
GeneID	853107.
KEGG	sce:YGR193C.
Organism-specific databases
CYGD	YGR193c.
SGD	S000003425. PDX1.
Phylogenomic databases
eggNOG	COG0508.
HOGENOM	HOG000246828.
OMA	EPIAYIA.
OrthoDB	EOG4QG0PK.
Gene expression databases
Genevestigator	P16451.
GermOnline	YGR193C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	4.10.320.10. 1 hit.
InterPro	IPR003016. 2-oxoA_DH_lipoyl-BS. IPR000089. Biotin_lipoyl. IPR004167. E3-bd. IPR011053. Single_hybrid_motif. [Graphical view]
Pfam	PF00364. Biotin_lipoyl. 1 hit. [Graphical view]
SUPFAM	SSF47005. E3_bd. 1 hit. SSF51230. Hybrid_motif. 1 hit.
PROSITE	PS50968. BIOTINYL_LIPOYL. 1 hit. PS00189. LIPOYL. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	973115.

Entry information

Entry name

ODPX_YEAST

Accession

Primary (citable) accession number: P16451
Secondary accession number(s): D6VUX5, E9P906

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	May 1, 2013
This is version 121 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program