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Protein

Pyruvate dehydrogenase complex protein X component, mitochondrial

Gene

PDX1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex.

GO - Molecular functioni

GO - Biological processi

  • acetyl-CoA biosynthetic process from pyruvate Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:YGR193C-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate dehydrogenase complex protein X component, mitochondrial
Alternative name(s):
Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex
E3-binding protein
Pyruvate dehydrogenase complex component E3BP
Gene namesi
Name:PDX1
Ordered Locus Names:YGR193C
ORF Names:G7579
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR193C.
SGDiS000003425. PDX1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial pyruvate dehydrogenase complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 30Mitochondrion1 PublicationAdd BLAST30
ChainiPRO_000002048731 – 410Pyruvate dehydrogenase complex protein X component, mitochondrialAdd BLAST380

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei73N6-lipoyllysinePROSITE-ProRule annotationBy similarity1

Proteomic databases

MaxQBiP16451.
PRIDEiP16451.

PTM databases

SwissPalmiP16451.

Interactioni

Subunit structurei

Eukaryotic pyruvate dehydrogenase (PDH) complexes are organized as a core consisting of the oligomeric dihydrolipoamide acetyl-transferase (E2), around which are arranged multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide dehydrogenase (E3) and protein X (E3BP) bound by non-covalent bonds.

Protein-protein interaction databases

BioGridi33446. 35 interactors.
DIPiDIP-5550N.
IntActiP16451. 8 interactors.
MINTiMINT-508924.

Structurei

3D structure databases

ProteinModelPortaliP16451.
SMRiP16451.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini32 – 108Lipoyl-bindingPROSITE-ProRule annotationAdd BLAST77

Sequence similaritiesi

Belongs to the 2-oxoacid dehydrogenase family.Curated
Contains 1 lipoyl-binding domain.PROSITE-ProRule annotationCurated

Keywords - Domaini

Lipoyl, Transit peptide

Phylogenomic databases

HOGENOMiHOG000246828.
InParanoidiP16451.
OMAiEPIAYIA.
OrthoDBiEOG092C3YQ0.

Family and domain databases

Gene3Di4.10.320.10. 1 hit.
InterProiIPR003016. 2-oxoA_DH_lipoyl-BS.
IPR000089. Biotin_lipoyl.
IPR004167. E3-bd.
IPR011053. Single_hybrid_motif.
[Graphical view]
PfamiPF00364. Biotin_lipoyl. 1 hit.
PF02817. E3_binding. 1 hit.
[Graphical view]
SUPFAMiSSF47005. SSF47005. 1 hit.
SSF51230. SSF51230. 1 hit.
PROSITEiPS50968. BIOTINYL_LIPOYL. 1 hit.
PS00189. LIPOYL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P16451-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSAISKVST LKSCTRYLTK CNYHASAKLL AVKTFSMPAM SPTMEKGGIV
60 70 80 90 100
SWKYKVGEPF SAGDVILEVE TDKSQIDVEA LDDGKLAKIL KDEGSKDVDV
110 120 130 140 150
GEPIAYIADV DDDLATIKLP QEANTANAKS IEIKKPSADS TEATQQHLKK
160 170 180 190 200
ATVTPIKTVD GSQANLEQTL LPSVSLLLAE NNISKQKALK EIAPSGSNGR
210 220 230 240 250
LLKGDVLAYL GKIPQDSVNK VTEFIKKNER LDLSNIKPIQ LKPKIAEQAQ
260 270 280 290 300
TKAADKPKIT PVEFEEQLVF HAPASIPFDK LSESLNSFMK EAYQFSHGTP
310 320 330 340 350
LMDTNSKYFD PIFEDLVTLS PREPRFKFSY DLMQIPKANN MQDTYGQEDI
360 370 380 390 400
FDLLTGSDAT ASSVRPVEKN LPEKNEYILA LNVSVNNKKF NDAEAKAKRF
410
LDYVRELESF
Length:410
Mass (Da):45,362
Last modified:August 1, 1990 - v1
Checksum:i11649CA28C420CDF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti378I → M in AAT93002 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28222 Genomic DNA. Translation: AAA34910.1.
X82408 Genomic DNA. Translation: CAA57804.1.
Z72978 Genomic DNA. Translation: CAA97219.1.
AY692983 Genomic DNA. Translation: AAT93002.1.
BK006941 Genomic DNA. Translation: DAA08286.1.
PIRiA36183. DEBYPX.
RefSeqiNP_011709.1. NM_001181322.1.

Genome annotation databases

EnsemblFungiiYGR193C; YGR193C; YGR193C.
GeneIDi853107.
KEGGisce:YGR193C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28222 Genomic DNA. Translation: AAA34910.1.
X82408 Genomic DNA. Translation: CAA57804.1.
Z72978 Genomic DNA. Translation: CAA97219.1.
AY692983 Genomic DNA. Translation: AAT93002.1.
BK006941 Genomic DNA. Translation: DAA08286.1.
PIRiA36183. DEBYPX.
RefSeqiNP_011709.1. NM_001181322.1.

3D structure databases

ProteinModelPortaliP16451.
SMRiP16451.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33446. 35 interactors.
DIPiDIP-5550N.
IntActiP16451. 8 interactors.
MINTiMINT-508924.

PTM databases

SwissPalmiP16451.

Proteomic databases

MaxQBiP16451.
PRIDEiP16451.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR193C; YGR193C; YGR193C.
GeneIDi853107.
KEGGisce:YGR193C.

Organism-specific databases

EuPathDBiFungiDB:YGR193C.
SGDiS000003425. PDX1.

Phylogenomic databases

HOGENOMiHOG000246828.
InParanoidiP16451.
OMAiEPIAYIA.
OrthoDBiEOG092C3YQ0.

Enzyme and pathway databases

BioCyciYEAST:YGR193C-MONOMER.

Miscellaneous databases

PROiP16451.

Family and domain databases

Gene3Di4.10.320.10. 1 hit.
InterProiIPR003016. 2-oxoA_DH_lipoyl-BS.
IPR000089. Biotin_lipoyl.
IPR004167. E3-bd.
IPR011053. Single_hybrid_motif.
[Graphical view]
PfamiPF00364. Biotin_lipoyl. 1 hit.
PF02817. E3_binding. 1 hit.
[Graphical view]
SUPFAMiSSF47005. SSF47005. 1 hit.
SSF51230. SSF51230. 1 hit.
PROSITEiPS50968. BIOTINYL_LIPOYL. 1 hit.
PS00189. LIPOYL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiODPX_YEAST
AccessioniPrimary (citable) accession number: P16451
Secondary accession number(s): D6VUX5, E9P906
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: November 2, 2016
This is version 152 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 414 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.