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Protein

Phosphatidylinositol transfer protein alpha isoform

Gene

Pitpna

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of PtdIns and phosphatidylcholine between membranes.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei59 – 591Phosphatidylinositol lipid headgroupBy similarity
Binding sitei61 – 611Phosphatidylinositol lipid headgroupBy similarity
Binding sitei86 – 861Phosphatidylinositol lipid headgroupBy similarity
Binding sitei90 – 901Phosphatidylinositol lipid headgroupBy similarity
Binding sitei97 – 971Phosphatidylinositol lipid headgroupBy similarity
Binding sitei195 – 1951Phosphatidylinositol lipid headgroupBy similarity

GO - Molecular functioni

  • fatty-acyl-CoA binding Source: UniProtKB
  • phosphatidylinositol binding Source: UniProtKB
  • phosphatidylinositol transporter activity Source: UniProtKB
  • phospholipid binding Source: MGI
  • stearic acid binding Source: UniProtKB

GO - Biological processi

  • phospholipid transport Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Transport

Keywords - Ligandi

Lipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol transfer protein alpha isoform
Short name:
PI-TP-alpha
Short name:
PtdIns transfer protein alpha
Short name:
PtdInsTP alpha
Gene namesi
Name:Pitpna
Synonyms:Pitpn
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi61885. Pitpna.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 271270Phosphatidylinositol transfer protein alpha isoformPRO_0000191642Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei216 – 2161N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP16446.
PRIDEiP16446.

2D gel databases

World-2DPAGE0004:P16446.

PTM databases

iPTMnetiP16446.
PhosphoSiteiP16446.

Expressioni

Tissue specificityi

Expressed in a wide range of tissues.

Gene expression databases

ExpressionAtlasiP16446. baseline and differential.

Interactioni

Protein-protein interaction databases

BioGridi248161. 1 interaction.
STRINGi10116.ENSRNOP00000005368.

Structurei

Secondary structure

1
271
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 1311Combined sources
Helixi15 – 3218Combined sources
Beta strandi38 – 4912Combined sources
Beta strandi55 – 6410Combined sources
Turni66 – 683Combined sources
Helixi71 – 744Combined sources
Turni79 – 824Combined sources
Beta strandi84 – 918Combined sources
Beta strandi94 – 1007Combined sources
Turni102 – 1043Combined sources
Helixi105 – 1073Combined sources
Beta strandi108 – 11912Combined sources
Turni124 – 1274Combined sources
Helixi133 – 1364Combined sources
Beta strandi138 – 1425Combined sources
Helixi147 – 1493Combined sources
Turni152 – 1543Combined sources
Helixi157 – 1593Combined sources
Turni161 – 1633Combined sources
Turni167 – 1693Combined sources
Helixi178 – 1825Combined sources
Beta strandi186 – 1883Combined sources
Beta strandi191 – 20111Combined sources
Turni204 – 2063Combined sources
Helixi207 – 23125Combined sources
Helixi233 – 2364Combined sources
Helixi241 – 26020Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1T27X-ray2.20A1-271[»]
ProteinModelPortaliP16446.
SMRiP16446. Positions 2-270.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP16446.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3668. Eukaryota.
COG5083. LUCA.
GeneTreeiENSGT00760000119216.
HOGENOMiHOG000006717.
HOVERGENiHBG058915.
InParanoidiP16446.
OrthoDBiEOG7PGDRB.
PhylomeDBiP16446.
TreeFamiTF313279.

Family and domain databases

Gene3Di3.30.530.20. 1 hit.
InterProiIPR001666. PI_transfer.
IPR023393. START-like_dom.
[Graphical view]
PANTHERiPTHR10658. PTHR10658. 1 hit.
PfamiPF02121. IP_trans. 1 hit.
[Graphical view]
PRINTSiPR00391. PITRANSFER.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P16446-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLLKEYRVI LPVSVDEYQV GQLYSVAEAS KNETGGGEGV EVLVNEPYEK
60 70 80 90 100
DDGEKGQYTH KIYHLQSKVP TFVRMLAPEG ALNIHEKAWN AYPYCRTVIT
110 120 130 140 150
NEYMKEDFLI KIETWHKPDL GTQENVHKLE PEAWKHVEAI YIDIADRSQV
160 170 180 190 200
LSKDYKAEED PAKFKSIKTG RGPLGPNWKQ ELVNQKDCPY MCAYKLVTVK
210 220 230 240 250
FKWWGLQNKV ENFIHKQEKR LFTNFHRQLF CWLDKWVDLT MDDIRRMEEE
260 270
TKRQLDEMRQ KDPVKGMTAD D
Length:271
Mass (Da):31,907
Last modified:January 23, 2007 - v2
Checksum:iF06794CD1930EDA9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti14 – 141S → H AA sequence (PubMed:3606604).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25758 mRNA. Translation: AAA41984.1.
BC070945 mRNA. Translation: AAH70945.1.
PIRiA34391.
RefSeqiNP_058927.1. NM_017231.1.
UniGeneiRn.9771.

Genome annotation databases

EnsembliENSRNOT00000090554; ENSRNOP00000072145; ENSRNOG00000003846.
GeneIDi29525.
KEGGirno:29525.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25758 mRNA. Translation: AAA41984.1.
BC070945 mRNA. Translation: AAH70945.1.
PIRiA34391.
RefSeqiNP_058927.1. NM_017231.1.
UniGeneiRn.9771.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1T27X-ray2.20A1-271[»]
ProteinModelPortaliP16446.
SMRiP16446. Positions 2-270.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248161. 1 interaction.
STRINGi10116.ENSRNOP00000005368.

PTM databases

iPTMnetiP16446.
PhosphoSiteiP16446.

2D gel databases

World-2DPAGE0004:P16446.

Proteomic databases

PaxDbiP16446.
PRIDEiP16446.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000090554; ENSRNOP00000072145; ENSRNOG00000003846.
GeneIDi29525.
KEGGirno:29525.

Organism-specific databases

CTDi5306.
RGDi61885. Pitpna.

Phylogenomic databases

eggNOGiKOG3668. Eukaryota.
COG5083. LUCA.
GeneTreeiENSGT00760000119216.
HOGENOMiHOG000006717.
HOVERGENiHBG058915.
InParanoidiP16446.
OrthoDBiEOG7PGDRB.
PhylomeDBiP16446.
TreeFamiTF313279.

Miscellaneous databases

EvolutionaryTraceiP16446.
PROiP16446.

Gene expression databases

ExpressionAtlasiP16446. baseline and differential.

Family and domain databases

Gene3Di3.30.530.20. 1 hit.
InterProiIPR001666. PI_transfer.
IPR023393. START-like_dom.
[Graphical view]
PANTHERiPTHR10658. PTHR10658. 1 hit.
PfamiPF02121. IP_trans. 1 hit.
[Graphical view]
PRINTSiPR00391. PITRANSFER.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and sequence of cDNA clones encoding rat phosphatidylinositol transfer protein."
    Dickeson S.K., Lim C.N., Schuyler G.T., Dalton T.P., Helmkamp G.M. Jr., Yarbrough L.R.
    J. Biol. Chem. 264:16557-16564(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart.
  3. "Amino-terminal sequence of a phospholipid transfer protein from rat lung."
    Funkhouser J.D.
    Biochem. Biophys. Res. Commun. 145:1310-1314(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-21.
    Tissue: Lung.
  4. Lubec G., Afjehi-Sadat L., Shim K.
    Submitted (DEC-2006) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 9-31; 88-96; 136-147 AND 235-244, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Spinal cord.
  5. "Structure of a multifunctional protein. Mammalian phosphatidylinositol transfer protein complexed with phosphatidylcholine."
    Yoder M.D., Thomas L.M., Tremblay J.M., Oliver R.L., Yarbrough L.R., Helmkamp G.M. Jr.
    J. Biol. Chem. 276:9246-9252(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).

Entry informationi

Entry nameiPIPNA_RAT
AccessioniPrimary (citable) accession number: P16446
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.