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Protein

Formate hydrogenlyase subunit 7

Gene

hycG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Cofactori

[4Fe-4S] clusterCuratedNote: Binds 1 [4Fe-4S] cluster.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi45Iron-sulfur (4Fe-4S)Sequence analysis1
Metal bindingi51Iron-sulfur (4Fe-4S)Sequence analysis1
Metal bindingi115Iron-sulfur (4Fe-4S)Sequence analysis1
Metal bindingi145Iron-sulfur (4Fe-4S)Sequence analysis1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:HYCG-MONOMER.
ECOL316407:JW2689-MONOMER.
MetaCyc:HYCG-MONOMER.

Protein family/group databases

TCDBi3.D.1.9.2. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate hydrogenlyase subunit 7
Short name:
FHL subunit 7
Alternative name(s):
Hydrogenase-3 component G
Gene namesi
Name:hycG
Synonyms:hevG
Ordered Locus Names:b2719, JW2689
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10480. hycG.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001187801 – 255Formate hydrogenlyase subunit 7Add BLAST255

Proteomic databases

PaxDbiP16433.
PRIDEiP16433.

Interactioni

Subunit structurei

FHL comprises of a formate dehydrogenase, unidentified electron carriers and a hydrogenase (isoenzyme 3). In this non-energy conserving pathway molecular hydrogen and carbodioxide from formate are released.

Binary interactionsi

WithEntry#Exp.IntActNotes
fdhFP076583EBI-541977,EBI-1121603
metNP307505EBI-541977,EBI-541886
mntRP0A9F14EBI-541977,EBI-541895

Protein-protein interaction databases

BioGridi4262939. 15 interactors.
DIPiDIP-9977N.
IntActiP16433. 10 interactors.
MINTiMINT-1287919.
STRINGi511145.b2719.

Structurei

3D structure databases

ProteinModelPortaliP16433.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 20 kDa subunit family.Curated

Phylogenomic databases

eggNOGiENOG4105C47. Bacteria.
COG3260. LUCA.
HOGENOMiHOG000228250.
InParanoidiP16433.
KOiK15832.
OMAiCIAYGAC.
PhylomeDBiP16433.

Family and domain databases

Gene3Di3.40.50.700. 1 hit.
InterProiIPR006137. NADH_UbQ_OxRdtase-like_20kDa.
IPR006138. NADH_UQ_OxRdtase_20Kd_su.
[Graphical view]
PfamiPF01058. Oxidored_q6. 1 hit.
[Graphical view]
PROSITEiPS01150. COMPLEX1_20K. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P16433-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNLLGPRDA NGIPVPMTVD ESIASMKASL LKKIKRSAYV YRVDCGGCNG
60 70 80 90 100
CEIEIFATLS PLFDAERFGI KVVPSPRHAD ILLFTGAVTR AMRSPALRAW
110 120 130 140 150
QSAPDPKICI SYGACGNSGG IFHDLYCVWG GTDKIVPVDV YIPGCPPTPA
160 170 180 190 200
ATLYGFAMAL GLLEQKIHAR GPGELDEQPA EILHGDMVQP LRVKVDREAR
210 220 230 240 250
RLAGYRYGRQ IADDYLTQLG QGEEQVARWL EAENDPRLNE IVSHLNHVVE

EARIR
Length:255
Mass (Da):27,999
Last modified:November 1, 1997 - v2
Checksum:iB24FBAE3C44F3A10
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti57A → G in CAA35552 (PubMed:2187144).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17506 Genomic DNA. Translation: CAA35552.1.
U29579 Genomic DNA. Translation: AAA69229.1.
U00096 Genomic DNA. Translation: AAC75761.1.
AP009048 Genomic DNA. Translation: BAE76796.1.
PIRiS08625.
RefSeqiNP_417199.1. NC_000913.3.
WP_000067392.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75761; AAC75761; b2719.
BAE76796; BAE76796; BAE76796.
GeneIDi947191.
KEGGiecj:JW2689.
eco:b2719.
PATRICi32120838. VBIEscCol129921_2811.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17506 Genomic DNA. Translation: CAA35552.1.
U29579 Genomic DNA. Translation: AAA69229.1.
U00096 Genomic DNA. Translation: AAC75761.1.
AP009048 Genomic DNA. Translation: BAE76796.1.
PIRiS08625.
RefSeqiNP_417199.1. NC_000913.3.
WP_000067392.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP16433.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262939. 15 interactors.
DIPiDIP-9977N.
IntActiP16433. 10 interactors.
MINTiMINT-1287919.
STRINGi511145.b2719.

Protein family/group databases

TCDBi3.D.1.9.2. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PaxDbiP16433.
PRIDEiP16433.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75761; AAC75761; b2719.
BAE76796; BAE76796; BAE76796.
GeneIDi947191.
KEGGiecj:JW2689.
eco:b2719.
PATRICi32120838. VBIEscCol129921_2811.

Organism-specific databases

EchoBASEiEB0475.
EcoGeneiEG10480. hycG.

Phylogenomic databases

eggNOGiENOG4105C47. Bacteria.
COG3260. LUCA.
HOGENOMiHOG000228250.
InParanoidiP16433.
KOiK15832.
OMAiCIAYGAC.
PhylomeDBiP16433.

Enzyme and pathway databases

BioCyciEcoCyc:HYCG-MONOMER.
ECOL316407:JW2689-MONOMER.
MetaCyc:HYCG-MONOMER.

Miscellaneous databases

PROiP16433.

Family and domain databases

Gene3Di3.40.50.700. 1 hit.
InterProiIPR006137. NADH_UbQ_OxRdtase-like_20kDa.
IPR006138. NADH_UQ_OxRdtase_20Kd_su.
[Graphical view]
PfamiPF01058. Oxidored_q6. 1 hit.
[Graphical view]
PROSITEiPS01150. COMPLEX1_20K. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHYCG_ECOLI
AccessioniPrimary (citable) accession number: P16433
Secondary accession number(s): Q2MAB0, Q46881
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.