P16433 (HYCG_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formate hydrogenlyase subunit 7
Short name=FHL subunit 7

Alternative name(s):
Hydrogenase-3 component G

Gene names

Name:	hycG
Synonyms:	hevG
Ordered Locus Names:	b2719, JW2689

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	255 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Cofactor	Binds 1 4Fe-4S cluster Potential.
Subunit structure	FHL comprises of a formate dehydrogenase, unidentified electron carriers and a hydrogenase (isoenzyme 3). In this non-energy conserving pathway molecular hydrogen and carbodioxide from formate are released.
Sequence similarities	Belongs to the complex I 20 kDa subunit family.

Ontologies

Keywords
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
lpoA	P45464	1	EBI-541977,EBI-557795

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 255	255	Formate hydrogenlyase subunit 7		PRO_0000118780
Sites
	Metal binding	45	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	51	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	115	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	145	1	Iron-sulfur (4Fe-4S) Potential
Experimental info
	Sequence conflict	57	1	A → G in CAA35552. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P16433 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: B24FBAE3C44F3A10	FASTA	255	27,999
	10 20 30 40 50 60 MSNLLGPRDA NGIPVPMTVD ESIASMKASL LKKIKRSAYV YRVDCGGCNG CEIEIFATLS 70 80 90 100 110 120 PLFDAERFGI KVVPSPRHAD ILLFTGAVTR AMRSPALRAW QSAPDPKICI SYGACGNSGG 130 140 150 160 170 180 IFHDLYCVWG GTDKIVPVDV YIPGCPPTPA ATLYGFAMAL GLLEQKIHAR GPGELDEQPA 190 200 210 220 230 240 EILHGDMVQP LRVKVDREAR RLAGYRYGRQ IADDYLTQLG QGEEQVARWL EAENDPRLNE 250 IVSHLNHVVE EARIR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence and expression of an operon in Escherichia coli coding for formate hydrogenlyase components." Boehm R., Sauter M., Boeck A. Mol. Microbiol. 4:231-243(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X17506 Genomic DNA. Translation: CAA35552.1. U29579 Genomic DNA. Translation: AAA69229.1. U00096 Genomic DNA. Translation: AAC75761.1. AP009048 Genomic DNA. Translation: BAE76796.1.
PIR	S08625.
RefSeq	NP_417199.1. NC_000913.2. YP_490928.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P16433.
SMR	P16433. Positions 77-178.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-9977N.
IntAct	P16433. 5 interactions.
MINT	MINT-1287919.
STRING	511145.b2719.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC75761; AAC75761; b2719. BAE76796; BAE76796; BAE76796.
GeneID	12933945. 947191.
KEGG	ecj:Y75_p2657. eco:b2719.
PATRIC	32120838. VBIEscCol129921_2811.
Organism-specific databases
EchoBASE	EB0475.
EcoGene	EG10480. hycG.
Phylogenomic databases
eggNOG	COG3260.
HOGENOM	HOG000228250.
KO	K15832.
OMA	TIYGFAM.
ProtClustDB	CLSK880465.
Enzyme and pathway databases
BioCyc	EcoCyc:HYCG-MONOMER. ECOL316407:JW2689-MONOMER. MetaCyc:HYCG-MONOMER.
Gene expression databases
Genevestigator	P16433.
Family and domain databases
Gene3D	3.40.50.700. 1 hit.
InterPro	IPR006137. NADH_UbQ_OxRdtase-like_20kDa. IPR006138. NADH_UQ_OxRdtase_20Kd_su. IPR014406. NiFe-hyd_3_ssu/Q_oxred_NuoB. [Graphical view]
PANTHER	PTHR11995. PTHR11995. 1 hit.
Pfam	PF01058. Oxidored_q6. 1 hit. [Graphical view]
PROSITE	PS01150. COMPLEX1_20K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HYCG_ECOLI

Accession

Primary (citable) accession number: P16433
Secondary accession number(s): Q2MAB0, Q46881

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 115 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program