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Protein

Formate hydrogenlyase subunit 6

Gene

hycF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Probable electron transfer protein for hydrogenase 3.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi40Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi43Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi46Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi50Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi75Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi78Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi81Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi85Iron-sulfur 2 (4Fe-4S)By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:HYCF-MONOMER.
ECOL316407:JW2690-MONOMER.
MetaCyc:HYCF-MONOMER.

Protein family/group databases

TCDBi3.D.1.9.2. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate hydrogenlyase subunit 6
Short name:
FHL subunit 6
Alternative name(s):
Hydrogenase-3 component F
Gene namesi
Name:hycF
Synonyms:hevF
Ordered Locus Names:b2720, JW2690
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10479. hycF.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001592661 – 180Formate hydrogenlyase subunit 6Add BLAST180

Proteomic databases

PaxDbiP16432.
PRIDEiP16432.

Interactioni

Subunit structurei

FHL comprises of a formate dehydrogenase, unidentified electron carriers and a hydrogenase (isoenzyme 3). In this non-energy conserving pathway, molecular hydrogen and carbodioxide are released from formate.

Protein-protein interaction databases

BioGridi4262940. 11 interactors.
IntActiP16432. 10 interactors.
STRINGi511145.b2720.

Structurei

3D structure databases

ProteinModelPortaliP16432.
SMRiP16432.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 604Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd BLAST30
Domaini66 – 954Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd BLAST30

Sequence similaritiesi

Contains 2 4Fe-4S ferredoxin-type domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4108RG7. Bacteria.
COG1143. LUCA.
HOGENOMiHOG000278697.
InParanoidiP16432.
KOiK15831.
OMAiHHRESFE.
PhylomeDBiP16432.

Family and domain databases

InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
[Graphical view]
PfamiPF12838. Fer4_7. 1 hit.
[Graphical view]
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P16432-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFTFIKKVIK TGTATSSYPL EPIAVDKNFR GKPEQNPQQC IGCAACVNAC
60 70 80 90 100
PSNALTVETD LATGELAWEF NLGHCIFCGR CEEVCPTAAI KLSQEYELAV
110 120 130 140 150
WKKEDFLQQS RFALCNCRVC NRPFAVQKEI DYAIALLKHN GDSRAENHRE
160 170 180
SFETCPECKR QKCLVPSDRI ELTRHMKEAI
Length:180
Mass (Da):20,309
Last modified:August 1, 1990 - v1
Checksum:i284B8C507B47DFDC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17506 Genomic DNA. Translation: CAA35551.1.
U29579 Genomic DNA. Translation: AAA69230.1.
U00096 Genomic DNA. Translation: AAC75762.1.
AP009048 Genomic DNA. Translation: BAE76797.1.
PIRiS08624.
RefSeqiNP_417200.1. NC_000913.3.
WP_000493781.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75762; AAC75762; b2720.
BAE76797; BAE76797; BAE76797.
GeneIDi947048.
KEGGiecj:JW2690.
eco:b2720.
PATRICi32120840. VBIEscCol129921_2812.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17506 Genomic DNA. Translation: CAA35551.1.
U29579 Genomic DNA. Translation: AAA69230.1.
U00096 Genomic DNA. Translation: AAC75762.1.
AP009048 Genomic DNA. Translation: BAE76797.1.
PIRiS08624.
RefSeqiNP_417200.1. NC_000913.3.
WP_000493781.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP16432.
SMRiP16432.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262940. 11 interactors.
IntActiP16432. 10 interactors.
STRINGi511145.b2720.

Protein family/group databases

TCDBi3.D.1.9.2. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PaxDbiP16432.
PRIDEiP16432.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75762; AAC75762; b2720.
BAE76797; BAE76797; BAE76797.
GeneIDi947048.
KEGGiecj:JW2690.
eco:b2720.
PATRICi32120840. VBIEscCol129921_2812.

Organism-specific databases

EchoBASEiEB0474.
EcoGeneiEG10479. hycF.

Phylogenomic databases

eggNOGiENOG4108RG7. Bacteria.
COG1143. LUCA.
HOGENOMiHOG000278697.
InParanoidiP16432.
KOiK15831.
OMAiHHRESFE.
PhylomeDBiP16432.

Enzyme and pathway databases

BioCyciEcoCyc:HYCF-MONOMER.
ECOL316407:JW2690-MONOMER.
MetaCyc:HYCF-MONOMER.

Miscellaneous databases

PROiP16432.

Family and domain databases

InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
[Graphical view]
PfamiPF12838. Fer4_7. 1 hit.
[Graphical view]
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHYCF_ECOLI
AccessioniPrimary (citable) accession number: P16432
Secondary accession number(s): Q2MAA9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: November 2, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.