Reviewed, UniProtKB/Swiss-Prot P16431 (HYCE_ECOLI)

Names and origin

Protein names

Recommended name:
    Formate hydrogenlyase subunit 5
      Short name=FHL subunit 5
Alternative name(s):
    Hydrogenase-3 component E

Gene names

Name:	hycE
Synonyms:	hevE
Ordered Locus Names:	b2721, JW2691

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	569 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Cofactor	Binds 1 4Fe-4S cluster Potential. Nickel Potential.
Subunit structure	FHL comprises of a formate dehydrogenase, unidentified electron carriers and a hydrogenase (isoenzyme 3). In this non-energy conserving pathway molecular hydrogen and carbodioxide from formate are released.
Sequence similarities	Belongs to the complex I 49 kDa subunit family.

Ontologies

Keywords
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding NAD Nickel
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NAD or NADH binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW nickel ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 537	537	Formate hydrogenlyase subunit 5		PRO_0000019985
	Propeptide	538 – 569	32			PRO_0000019986

Sequences

	Sequence		Length	Mass (Da)
	P16431-1 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: 38A2BCCB01B27463	FASTA	569	64,980
	10 20 30 40 50 60 MSEEKLGQHY LAALNEAFPG VVLDHAWQTK DQLTVTVKVN YLPEVVEFLY YKQGGWLSVL 70 80 90 100 110 120 FGNDERKLNG HYAVYYVLSM EKGTKCWITV RVEVDANKPE YPSVTPRVPA AVWGEREVRD 130 140 150 160 170 180 MYGLIPVGLP DERRLVLPDD WPDELYPLRK DSMDYRQRPA PTTDAETYEF INELGDKKNN 190 200 210 220 230 240 VVPIGPLHVT SDEPGHFRLF VDGENIIDAD YRLFYVHRGM EKLAETRMGY NEVTFLSDRV 250 260 270 280 290 300 CGICGFAHST AYTTSVENAM GIQVPERAQM IRAILLEVER LHSHLLNLGL ACHFTGFDSG 310 320 330 340 350 360 FMQFFRVRET SMKMAEILTG ARKTYGLNLI GGIRRDLLKD DMIQTRQLAQ QMRREVQELV 370 380 390 400 410 420 DVLLSTPNME QRTVGIGRLD PEIARDFSNV GPMVRASGHA RDTRADHPFV GYGLLPMEVH 430 440 450 460 470 480 SEQGCDVISR LKVRINEVYT ALNMIDYGLD NLPGGPLMVE GFTYIPHRFA LGFAEAPRGD 490 500 510 520 530 540 DIHWSMTGDN QKLYRWRCRA ATYANWPTLR YMLRGNTVSD APLIIGSLDP CYSCTDRMTV 550 560 VDVRKKKSKV VPYKELERYS IERKNSPLK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence and expression of an operon in Escherichia coli coding for formate hydrogenlyase components." Boehm R., Sauter M., Boeck A. Mol. Microbiol. 4:231-243(1990) [PubMed: 2187144] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Maturation of the large subunit (HYCE) of Escherichia coli hydrogenase 3 requires nickel incorporation followed by C-terminal processing at Arg537." Rossmann R., Sauter M., Lottspeich F., Boeck A. Eur. J. Biochem. 220:377-384(1994) [PubMed: 8125094] [Abstract] Cited for: PROTEOLYTIC PROCESSING.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X17506 Genomic DNA. Translation: CAA35550.1. U29579 Genomic DNA. Translation: AAA69231.1. U00096 Genomic DNA. Translation: AAC75763.1. AP009048 Genomic DNA. Translation: BAE76798.1.
PIR	S08623.
RefSeq	AP_003288.1. NP_417201.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:9975N.
Genome annotation databases
GeneID	947396.
GenomeReviews	Gene locus JW2691 in contig AP009048_GR. Gene locus b2721 in contig U00096_GR.
KEGG	ecj:JW2691. eco:b2721.
Organism-specific databases
EchoBASE	EB0473.
EcoGene	EG10478. hycE.
CMR	Search...
Phylogenomic databases
HOGENOM	P16431.
OMA	P16431. SCTERVT.
Enzyme and pathway databases
BioCyc	EcoCyc:HYCELARGE-MON. MetaCyc:HYCELARGE-MON.
Family and domain databases
InterPro	IPR001135. NADH_Q_OxRdtase_suD. IPR001268. NADH_UbQ_OxRdtase_30kDa_su. IPR014029. NADH_UbQ_OxRdtase_su-49kDa_CS. IPR001501. Ni-dep_hyd_lsu. [Graphical view]
Pfam	PF00329. Complex1_30kDa. 1 hit. PF00346. Complex1_49kDa. 2 hits. PF00374. NiFeSe_Hases. 1 hit. [Graphical view]
ProDom	PD001581. Complex1_30K. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00535. COMPLEX1_49K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HYCE_ECOLI

Accession

Primary (citable) accession number: P16431
Secondary accession number(s): Q2MAA8

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	June 16, 2009
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)