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Protein

Formate hydrogenlyase subunit 5

Gene

hycE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Cofactori

Protein has several cofactor binding sites:

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Nickel

Enzyme and pathway databases

BioCyciEcoCyc:HYCELARGE-MONOMER.
ECOL316407:JW2691-MONOMER.
MetaCyc:HYCELARGE-MONOMER.

Protein family/group databases

TCDBi3.D.1.9.2. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate hydrogenlyase subunit 5
Short name:
FHL subunit 5
Alternative name(s):
Hydrogenase-3 component E
Gene namesi
Name:hycE
Synonyms:hevE
Ordered Locus Names:b2721, JW2691
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10478. hycE.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 537537Formate hydrogenlyase subunit 5PRO_0000019985Add
BLAST
Propeptidei538 – 56932PRO_0000019986Add
BLAST

Proteomic databases

PaxDbiP16431.
PRIDEiP16431.

Interactioni

Subunit structurei

FHL comprises of a formate dehydrogenase, unidentified electron carriers and a hydrogenase (isoenzyme 3). In this non-energy conserving pathway molecular hydrogen and carbodioxide from formate are released.

Binary interactionsi

WithEntry#Exp.IntActNotes
hypAP0A70014EBI-552702,EBI-6290024
slyDP0A9K97EBI-552702,EBI-369251

Protein-protein interaction databases

BioGridi4261447. 7 interactions.
DIPiDIP-9975N.
IntActiP16431. 18 interactions.
MINTiMINT-1223046.
STRINGi511145.b2721.

Structurei

3D structure databases

ProteinModelPortaliP16431.
SMRiP16431. Positions 90-151, 195-537.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 49 kDa subunit family.Curated

Phylogenomic databases

eggNOGiENOG4105CQV. Bacteria.
COG3261. LUCA.
COG3262. LUCA.
HOGENOMiHOG000228262.
InParanoidiP16431.
KOiK15830.
OMAiGEWRNEA.
OrthoDBiEOG6HF5VV.
PhylomeDBiP16431.

Family and domain databases

Gene3Di1.10.645.10. 1 hit.
InterProiIPR001135. NADH_Q_OxRdtase_suD.
IPR001268. NADH_UbQ_OxRdtase_30kDa_su.
IPR014029. NADH_UbQ_OxRdtase_49kDa_CS.
IPR001501. Ni-dep_hyd_lsu.
IPR029014. NiFe_Hase-like.
[Graphical view]
PfamiPF00329. Complex1_30kDa. 1 hit.
PF00346. Complex1_49kDa. 2 hits.
PF00374. NiFeSe_Hases. 1 hit.
[Graphical view]
ProDomiPD001581. NADH_UbQ_OxRdtase_30kDa_su. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF56762. SSF56762. 1 hit.
PROSITEiPS00535. COMPLEX1_49K. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P16431-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEEKLGQHY LAALNEAFPG VVLDHAWQTK DQLTVTVKVN YLPEVVEFLY
60 70 80 90 100
YKQGGWLSVL FGNDERKLNG HYAVYYVLSM EKGTKCWITV RVEVDANKPE
110 120 130 140 150
YPSVTPRVPA AVWGEREVRD MYGLIPVGLP DERRLVLPDD WPDELYPLRK
160 170 180 190 200
DSMDYRQRPA PTTDAETYEF INELGDKKNN VVPIGPLHVT SDEPGHFRLF
210 220 230 240 250
VDGENIIDAD YRLFYVHRGM EKLAETRMGY NEVTFLSDRV CGICGFAHST
260 270 280 290 300
AYTTSVENAM GIQVPERAQM IRAILLEVER LHSHLLNLGL ACHFTGFDSG
310 320 330 340 350
FMQFFRVRET SMKMAEILTG ARKTYGLNLI GGIRRDLLKD DMIQTRQLAQ
360 370 380 390 400
QMRREVQELV DVLLSTPNME QRTVGIGRLD PEIARDFSNV GPMVRASGHA
410 420 430 440 450
RDTRADHPFV GYGLLPMEVH SEQGCDVISR LKVRINEVYT ALNMIDYGLD
460 470 480 490 500
NLPGGPLMVE GFTYIPHRFA LGFAEAPRGD DIHWSMTGDN QKLYRWRCRA
510 520 530 540 550
ATYANWPTLR YMLRGNTVSD APLIIGSLDP CYSCTDRMTV VDVRKKKSKV
560
VPYKELERYS IERKNSPLK
Length:569
Mass (Da):64,980
Last modified:August 1, 1990 - v1
Checksum:i38A2BCCB01B27463
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17506 Genomic DNA. Translation: CAA35550.1.
U29579 Genomic DNA. Translation: AAA69231.1.
U00096 Genomic DNA. Translation: AAC75763.1.
AP009048 Genomic DNA. Translation: BAE76798.1.
PIRiS08623.
RefSeqiNP_417201.1. NC_000913.3.
WP_001288122.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75763; AAC75763; b2721.
BAE76798; BAE76798; BAE76798.
GeneIDi947396.
KEGGiecj:JW2691.
eco:b2721.
PATRICi32120842. VBIEscCol129921_2813.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17506 Genomic DNA. Translation: CAA35550.1.
U29579 Genomic DNA. Translation: AAA69231.1.
U00096 Genomic DNA. Translation: AAC75763.1.
AP009048 Genomic DNA. Translation: BAE76798.1.
PIRiS08623.
RefSeqiNP_417201.1. NC_000913.3.
WP_001288122.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP16431.
SMRiP16431. Positions 90-151, 195-537.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261447. 7 interactions.
DIPiDIP-9975N.
IntActiP16431. 18 interactions.
MINTiMINT-1223046.
STRINGi511145.b2721.

Protein family/group databases

TCDBi3.D.1.9.2. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PaxDbiP16431.
PRIDEiP16431.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75763; AAC75763; b2721.
BAE76798; BAE76798; BAE76798.
GeneIDi947396.
KEGGiecj:JW2691.
eco:b2721.
PATRICi32120842. VBIEscCol129921_2813.

Organism-specific databases

EchoBASEiEB0473.
EcoGeneiEG10478. hycE.

Phylogenomic databases

eggNOGiENOG4105CQV. Bacteria.
COG3261. LUCA.
COG3262. LUCA.
HOGENOMiHOG000228262.
InParanoidiP16431.
KOiK15830.
OMAiGEWRNEA.
OrthoDBiEOG6HF5VV.
PhylomeDBiP16431.

Enzyme and pathway databases

BioCyciEcoCyc:HYCELARGE-MONOMER.
ECOL316407:JW2691-MONOMER.
MetaCyc:HYCELARGE-MONOMER.

Miscellaneous databases

PROiP16431.

Family and domain databases

Gene3Di1.10.645.10. 1 hit.
InterProiIPR001135. NADH_Q_OxRdtase_suD.
IPR001268. NADH_UbQ_OxRdtase_30kDa_su.
IPR014029. NADH_UbQ_OxRdtase_49kDa_CS.
IPR001501. Ni-dep_hyd_lsu.
IPR029014. NiFe_Hase-like.
[Graphical view]
PfamiPF00329. Complex1_30kDa. 1 hit.
PF00346. Complex1_49kDa. 2 hits.
PF00374. NiFeSe_Hases. 1 hit.
[Graphical view]
ProDomiPD001581. NADH_UbQ_OxRdtase_30kDa_su. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF56762. SSF56762. 1 hit.
PROSITEiPS00535. COMPLEX1_49K. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence and expression of an operon in Escherichia coli coding for formate hydrogenlyase components."
    Boehm R., Sauter M., Boeck A.
    Mol. Microbiol. 4:231-243(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Maturation of the large subunit (HYCE) of Escherichia coli hydrogenase 3 requires nickel incorporation followed by C-terminal processing at Arg537."
    Rossmann R., Sauter M., Lottspeich F., Boeck A.
    Eur. J. Biochem. 220:377-384(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEOLYTIC PROCESSING.

Entry informationi

Entry nameiHYCE_ECOLI
AccessioniPrimary (citable) accession number: P16431
Secondary accession number(s): Q2MAA8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: March 16, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.