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Protein

Formate hydrogenlyase subunit 5

Gene

hycE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cofactori

Protein has several cofactor binding sites:

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Nickel

Enzyme and pathway databases

BioCyciEcoCyc:HYCELARGE-MONOMER
MetaCyc:HYCELARGE-MONOMER

Protein family/group databases

TCDBi3.D.1.9.2 the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family

Names & Taxonomyi

Protein namesi
Recommended name:
Formate hydrogenlyase subunit 5
Short name:
FHL subunit 5
Alternative name(s):
Hydrogenase-3 component E
Gene namesi
Name:hycE
Synonyms:hevE
Ordered Locus Names:b2721, JW2691
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10478 hycE

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000199851 – 537Formate hydrogenlyase subunit 5Add BLAST537
PropeptideiPRO_0000019986538 – 569Add BLAST32

Proteomic databases

PaxDbiP16431
PRIDEiP16431

Interactioni

Subunit structurei

FHL comprises of a formate dehydrogenase, unidentified electron carriers and a hydrogenase (isoenzyme 3). In this non-energy conserving pathway molecular hydrogen and carbodioxide from formate are released.

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi4261447, 11 interactors
DIPiDIP-9975N
IntActiP16431, 18 interactors
MINTiP16431
STRINGi316385.ECDH10B_2889

Structurei

3D structure databases

ProteinModelPortaliP16431
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 49 kDa subunit family.Curated

Phylogenomic databases

eggNOGiENOG4105CQV Bacteria
COG3261 LUCA
COG3262 LUCA
HOGENOMiHOG000228262
InParanoidiP16431
KOiK15830
OMAiCDPCFTC
PhylomeDBiP16431

Family and domain databases

Gene3Di1.10.645.20, 1 hit
3.30.460.80, 1 hit
InterProiView protein in InterPro
IPR001135 NADH_Q_OxRdtase_suD
IPR038290 NADH_Q_OxRdtase_suD_sf
IPR037232 NADH_quin_OxRdtase_su_C/D-like
IPR001268 NADH_UbQ_OxRdtase_30kDa_su
IPR014029 NADH_UbQ_OxRdtase_49kDa_CS
IPR001501 Ni-dep_hyd_lsu
IPR029014 NiFe-Hase_large
PfamiView protein in Pfam
PF00329 Complex1_30kDa, 1 hit
PF00346 Complex1_49kDa, 2 hits
PF00374 NiFeSe_Hases, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD001581 NADH_UbQ_OxRdtase_30kDa_su, 1 hit
SUPFAMiSSF143243 SSF143243, 1 hit
SSF56762 SSF56762, 1 hit
PROSITEiView protein in PROSITE
PS00535 COMPLEX1_49K, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P16431-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEEKLGQHY LAALNEAFPG VVLDHAWQTK DQLTVTVKVN YLPEVVEFLY
60 70 80 90 100
YKQGGWLSVL FGNDERKLNG HYAVYYVLSM EKGTKCWITV RVEVDANKPE
110 120 130 140 150
YPSVTPRVPA AVWGEREVRD MYGLIPVGLP DERRLVLPDD WPDELYPLRK
160 170 180 190 200
DSMDYRQRPA PTTDAETYEF INELGDKKNN VVPIGPLHVT SDEPGHFRLF
210 220 230 240 250
VDGENIIDAD YRLFYVHRGM EKLAETRMGY NEVTFLSDRV CGICGFAHST
260 270 280 290 300
AYTTSVENAM GIQVPERAQM IRAILLEVER LHSHLLNLGL ACHFTGFDSG
310 320 330 340 350
FMQFFRVRET SMKMAEILTG ARKTYGLNLI GGIRRDLLKD DMIQTRQLAQ
360 370 380 390 400
QMRREVQELV DVLLSTPNME QRTVGIGRLD PEIARDFSNV GPMVRASGHA
410 420 430 440 450
RDTRADHPFV GYGLLPMEVH SEQGCDVISR LKVRINEVYT ALNMIDYGLD
460 470 480 490 500
NLPGGPLMVE GFTYIPHRFA LGFAEAPRGD DIHWSMTGDN QKLYRWRCRA
510 520 530 540 550
ATYANWPTLR YMLRGNTVSD APLIIGSLDP CYSCTDRMTV VDVRKKKSKV
560
VPYKELERYS IERKNSPLK
Length:569
Mass (Da):64,980
Last modified:August 1, 1990 - v1
Checksum:i38A2BCCB01B27463
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17506 Genomic DNA Translation: CAA35550.1
U29579 Genomic DNA Translation: AAA69231.1
U00096 Genomic DNA Translation: AAC75763.1
AP009048 Genomic DNA Translation: BAE76798.1
PIRiS08623
RefSeqiNP_417201.1, NC_000913.3
WP_001288122.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75763; AAC75763; b2721
BAE76798; BAE76798; BAE76798
GeneIDi947396
KEGGiecj:JW2691
eco:b2721
PATRICifig|1411691.4.peg.4020

Similar proteinsi

Entry informationi

Entry nameiHYCE_ECOLI
AccessioniPrimary (citable) accession number: P16431
Secondary accession number(s): Q2MAA8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: March 28, 2018
This is version 148 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families
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