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Protein

Formate hydrogenlyase subunit 4

Gene

hycD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Enzyme and pathway databases

BioCyciEcoCyc:HYCD-MONOMER.
ECOL316407:JW2692-MONOMER.
MetaCyc:HYCD-MONOMER.

Protein family/group databases

TCDBi3.D.1.9.2. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate hydrogenlyase subunit 4
Short name:
FHL subunit 4
Alternative name(s):
Hydrogenase 3 component D
Gene namesi
Name:hycD
Synonyms:hevD
Ordered Locus Names:b2722, JW2692
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10477. hycD.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 22PeriplasmicSequence analysis
Transmembranei3 – 2321HelicalSequence analysisAdd
BLAST
Topological domaini24 – 6744CytoplasmicSequence analysisAdd
BLAST
Transmembranei68 – 8821HelicalSequence analysisAdd
BLAST
Topological domaini89 – 935PeriplasmicSequence analysis
Transmembranei94 – 11421HelicalSequence analysisAdd
BLAST
Topological domaini115 – 13117CytoplasmicSequence analysisAdd
BLAST
Transmembranei132 – 15221HelicalSequence analysisAdd
BLAST
Topological domaini153 – 16715PeriplasmicSequence analysisAdd
BLAST
Transmembranei168 – 18821HelicalSequence analysisAdd
BLAST
Topological domaini189 – 22133CytoplasmicSequence analysisAdd
BLAST
Transmembranei222 – 24221HelicalSequence analysisAdd
BLAST
Topological domaini243 – 25311PeriplasmicSequence analysisAdd
BLAST
Transmembranei254 – 27421HelicalSequence analysisAdd
BLAST
Topological domaini275 – 28410CytoplasmicSequence analysis
Transmembranei285 – 30521HelicalSequence analysisAdd
BLAST
Topological domaini306 – 3072PeriplasmicSequence analysis

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 307307Formate hydrogenlyase subunit 4PRO_0000117534Add
BLAST

Proteomic databases

PaxDbiP16430.

Interactioni

Subunit structurei

FHL comprises of a formate dehydrogenase, unidentified electron carriers and a hydrogenase (isoenzyme 3). In this non-energy conserving pathway molecular hydrogen and carbodioxide from formate are released.

Protein-protein interaction databases

BioGridi4261429. 6 interactions.
IntActiP16430. 1 interaction.
STRINGi511145.b2722.

Structurei

3D structure databases

ProteinModelPortaliP16430.
SMRiP16430. Positions 1-307.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I subunit 1 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4107TDF. Bacteria.
COG0650. LUCA.
HOGENOMiHOG000228277.
InParanoidiP16430.
KOiK15829.
OMAiVYHWPVA.
OrthoDBiEOG69PQ1G.
PhylomeDBiP16430.

Family and domain databases

InterProiIPR001694. NADH_UbQ_OxRdtase_su1/FPO.
IPR018086. NADH_UbQ_OxRdtase_su1_CS.
[Graphical view]
PANTHERiPTHR11432. PTHR11432. 1 hit.
PfamiPF00146. NADHdh. 1 hit.
[Graphical view]
PROSITEiPS00667. COMPLEX1_ND1_1. 1 hit.
PS00668. COMPLEX1_ND1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P16430-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVLYPLIQA LVLFAVAPLL SGITRVARAR LHNRRGPGVL QEYRDIIKLL
60 70 80 90 100
GRQSVGPDAS GWVFRLTPYV MVGVMLTIAT ALPVVTVGSP LPQLGDLITL
110 120 130 140 150
LYLFAIARFF FAISGLDTGS PFTAIGASRE AMLGVLVEPM LLLGLWVAAQ
160 170 180 190 200
VAGSTNISNI TDTVYHWPLS QSIPLVLALC ACAFATFIEM GKLPFDLAEA
210 220 230 240 250
EQELQEGPLS EYSGSGFGVM KWGISLKQLV VLQMFVGVFI PWGQMETFTA
260 270 280 290 300
GGLLLALVIA IVKLVVGVLV IALFENSMAR LRLDITPRIT WAGFGFAFLA

FVSLLAA
Length:307
Mass (Da):33,029
Last modified:August 1, 1990 - v1
Checksum:i2A3F410620E82AC9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17506 Genomic DNA. Translation: CAA35549.1.
U29579 Genomic DNA. Translation: AAA69232.1.
U00096 Genomic DNA. Translation: AAC75764.1.
AP009048 Genomic DNA. Translation: BAE76799.1.
PIRiS08622.
RefSeqiNP_417202.1. NC_000913.3.
WP_000115224.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75764; AAC75764; b2722.
BAE76799; BAE76799; BAE76799.
GeneIDi948994.
KEGGiecj:JW2692.
eco:b2722.
PATRICi32120844. VBIEscCol129921_2814.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17506 Genomic DNA. Translation: CAA35549.1.
U29579 Genomic DNA. Translation: AAA69232.1.
U00096 Genomic DNA. Translation: AAC75764.1.
AP009048 Genomic DNA. Translation: BAE76799.1.
PIRiS08622.
RefSeqiNP_417202.1. NC_000913.3.
WP_000115224.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP16430.
SMRiP16430. Positions 1-307.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261429. 6 interactions.
IntActiP16430. 1 interaction.
STRINGi511145.b2722.

Protein family/group databases

TCDBi3.D.1.9.2. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PaxDbiP16430.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75764; AAC75764; b2722.
BAE76799; BAE76799; BAE76799.
GeneIDi948994.
KEGGiecj:JW2692.
eco:b2722.
PATRICi32120844. VBIEscCol129921_2814.

Organism-specific databases

EchoBASEiEB0472.
EcoGeneiEG10477. hycD.

Phylogenomic databases

eggNOGiENOG4107TDF. Bacteria.
COG0650. LUCA.
HOGENOMiHOG000228277.
InParanoidiP16430.
KOiK15829.
OMAiVYHWPVA.
OrthoDBiEOG69PQ1G.
PhylomeDBiP16430.

Enzyme and pathway databases

BioCyciEcoCyc:HYCD-MONOMER.
ECOL316407:JW2692-MONOMER.
MetaCyc:HYCD-MONOMER.

Miscellaneous databases

PROiP16430.

Family and domain databases

InterProiIPR001694. NADH_UbQ_OxRdtase_su1/FPO.
IPR018086. NADH_UbQ_OxRdtase_su1_CS.
[Graphical view]
PANTHERiPTHR11432. PTHR11432. 1 hit.
PfamiPF00146. NADHdh. 1 hit.
[Graphical view]
PROSITEiPS00667. COMPLEX1_ND1_1. 1 hit.
PS00668. COMPLEX1_ND1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence and expression of an operon in Escherichia coli coding for formate hydrogenlyase components."
    Boehm R., Sauter M., Boeck A.
    Mol. Microbiol. 4:231-243(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiHYCD_ECOLI
AccessioniPrimary (citable) accession number: P16430
Secondary accession number(s): Q2MAA7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: March 16, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.