P16430 (HYCD_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formate hydrogenlyase subunit 4
Short name=FHL subunit 4

Alternative name(s):
Hydrogenase 3 component D

Gene names

Name:	hycD
Synonyms:	hevD
Ordered Locus Names:	b2722, JW2692

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	307 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	FHL comprises of a formate dehydrogenase, unidentified electron carriers and a hydrogenase (isoenzyme 3). In this non-energy conserving pathway molecular hydrogen and carbodioxide from formate are released.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the complex I subunit 1 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 307	307	Formate hydrogenlyase subunit 4		PRO_0000117534
Regions
	Topological domain	1 – 2	2	Periplasmic Potential
	Transmembrane	3 – 23	21	Helical; Potential
	Topological domain	24 – 67	44	Cytoplasmic Potential
	Transmembrane	68 – 88	21	Helical; Potential
	Topological domain	89 – 93	5	Periplasmic Potential
	Transmembrane	94 – 114	21	Helical; Potential
	Topological domain	115 – 131	17	Cytoplasmic Potential
	Transmembrane	132 – 152	21	Helical; Potential
	Topological domain	153 – 167	15	Periplasmic Potential
	Transmembrane	168 – 188	21	Helical; Potential
	Topological domain	189 – 221	33	Cytoplasmic Potential
	Transmembrane	222 – 242	21	Helical; Potential
	Topological domain	243 – 253	11	Periplasmic Potential
	Transmembrane	254 – 274	21	Helical; Potential
	Topological domain	275 – 284	10	Cytoplasmic Potential
	Transmembrane	285 – 305	21	Helical; Potential
	Topological domain	306 – 307	2	Periplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	P16430 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: 2A3F410620E82AC9	FASTA	307	33,029
	10 20 30 40 50 60 MSVLYPLIQA LVLFAVAPLL SGITRVARAR LHNRRGPGVL QEYRDIIKLL GRQSVGPDAS 70 80 90 100 110 120 GWVFRLTPYV MVGVMLTIAT ALPVVTVGSP LPQLGDLITL LYLFAIARFF FAISGLDTGS 130 140 150 160 170 180 PFTAIGASRE AMLGVLVEPM LLLGLWVAAQ VAGSTNISNI TDTVYHWPLS QSIPLVLALC 190 200 210 220 230 240 ACAFATFIEM GKLPFDLAEA EQELQEGPLS EYSGSGFGVM KWGISLKQLV VLQMFVGVFI 250 260 270 280 290 300 PWGQMETFTA GGLLLALVIA IVKLVVGVLV IALFENSMAR LRLDITPRIT WAGFGFAFLA FVSLLAA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence and expression of an operon in Escherichia coli coding for formate hydrogenlyase components." Boehm R., Sauter M., Boeck A. Mol. Microbiol. 4:231-243(1990) [PubMed: 2187144] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X17506 Genomic DNA. Translation: CAA35549.1. U29579 Genomic DNA. Translation: AAA69232.1. U00096 Genomic DNA. Translation: AAC75764.1. AP009048 Genomic DNA. Translation: BAE76799.1.
PIR	S08622.
RefSeq	NP_417202.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P16430.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000000443; EBESCP00000000443; EBESCG00000000366. EBESCT00000014953; EBESCP00000014244; EBESCG00000014013.
GeneID	948994.
GenomeReviews	Gene locus JW2692 in contig AP009048_GR. Gene locus b2722 in contig U00096_GR.
KEGG	ecj:JW2692. eco:b2722.
PATRIC	32120844. VBIEscCol129921_2814.
Organism-specific databases
EchoBASE	EB0472.
EcoGene	EG10477. hycD.
Phylogenomic databases
eggNOG	COG0650.
GeneTree	EBGT00050000009143.
HOGENOM	HBG727670.
OMA	FFPWGQM.
PhylomeDB	P16430.
ProtClustDB	CLSK880406.
Enzyme and pathway databases
BioCyc	EcoCyc:HYCD-MONOMER. MetaCyc:HYCD-MONOMER.
Gene expression databases
Genevestigator	P16430.
Family and domain databases
InterPro	IPR001694. NADH_UbQ_OxRdtase_su1/FPO. IPR018086. NADH_UbQ_OxRdtase_su1_CS. [Graphical view]
PANTHER	PTHR11432. Resp_NADH_DH_1. 1 hit.
Pfam	PF00146. NADHdh. 1 hit. [Graphical view]
PROSITE	PS00667. COMPLEX1_ND1_1. 1 hit. PS00668. COMPLEX1_ND1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HYCD_ECOLI

Accession

Primary (citable) accession number: P16430
Secondary accession number(s): Q2MAA7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	January 25, 2012
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program