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Reviewed, UniProtKB/Swiss-Prot P16430 (HYCD_ECOLI)

Last modified June 16, 2009. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Formate hydrogenlyase subunit 4
      Short name=FHL subunit 4
Alternative name(s):
    Hydrogenase 3 component D
Gene names
Name: hycD
Synonyms: hevD
Ordered Locus Names: b2722, JW2692
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length307 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Subunit structure

FHL comprises of a formate dehydrogenase, unidentified electron carriers and a hydrogenase (isoenzyme 3). In this non-energy conserving pathway molecular hydrogen and carbodioxide from formate are released.

Subcellular location

Cell inner membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the complex I subunit 1 family.

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Ontologies

Keywords
   Cellular componentCell inner membrane
Cell membrane
Membrane
   DomainTransmembrane
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processoxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionoxidoreductase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 307307Formate hydrogenlyase subunit 4
PRO_0000117534

Regions

Topological domain1 – 22Periplasmic Potential
Transmembrane3 – 2321 Potential
Topological domain24 – 6744Cytoplasmic Potential
Transmembrane68 – 8821 Potential
Topological domain89 – 935Periplasmic Potential
Transmembrane94 – 11421 Potential
Topological domain115 – 13117Cytoplasmic Potential
Transmembrane132 – 15221 Potential
Topological domain153 – 16715Periplasmic Potential
Transmembrane168 – 18821 Potential
Topological domain189 – 22133Cytoplasmic Potential
Transmembrane222 – 24221 Potential
Topological domain243 – 25311Periplasmic Potential
Transmembrane254 – 27421 Potential
Topological domain275 – 28410Cytoplasmic Potential
Transmembrane285 – 30521 Potential
Topological domain306 – 3072Periplasmic Potential

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Sequences

Sequence LengthMass (Da)Tools
P16430-1 [UniParc].

Last modified August 1, 1990. Version 1.
Checksum: 2A3F410620E82AC9

FASTA30733,029
        10         20         30         40         50         60 
MSVLYPLIQA LVLFAVAPLL SGITRVARAR LHNRRGPGVL QEYRDIIKLL GRQSVGPDAS 

        70         80         90        100        110        120 
GWVFRLTPYV MVGVMLTIAT ALPVVTVGSP LPQLGDLITL LYLFAIARFF FAISGLDTGS 

       130        140        150        160        170        180 
PFTAIGASRE AMLGVLVEPM LLLGLWVAAQ VAGSTNISNI TDTVYHWPLS QSIPLVLALC 

       190        200        210        220        230        240 
ACAFATFIEM GKLPFDLAEA EQELQEGPLS EYSGSGFGVM KWGISLKQLV VLQMFVGVFI 

       250        260        270        280        290        300 
PWGQMETFTA GGLLLALVIA IVKLVVGVLV IALFENSMAR LRLDITPRIT WAGFGFAFLA 


FVSLLAA

« Hide

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References

« Hide 'large scale' references
[1]"Nucleotide sequence and expression of an operon in Escherichia coli coding for formate hydrogenlyase components."
Boehm R., Sauter M., Boeck A.
Mol. Microbiol. 4:231-243(1990) [PubMed: 2187144] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"Global topology analysis of the Escherichia coli inner membrane proteome."
Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: K12 / MG1655 / ATCC 47076.

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Cross-references

Sequence databases

X17506 Genomic DNA. Translation: CAA35549.1.
U29579 Genomic DNA. Translation: AAA69232.1.
U00096 Genomic DNA. Translation: AAC75764.1.
AP009048 Genomic DNA. Translation: BAE76799.1.
PIRS08622.
RefSeqAP_003289.1.
NP_417202.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID948994.
GenomeReviewsGene locus JW2692 in contig AP009048_GR.
Gene locus b2722 in contig U00096_GR.
KEGGecj:JW2692.
eco:b2722.

Organism-specific databases

EchoBASEEB0472.
EcoGeneEG10477. hycD.
CMRSearch...

Phylogenomic databases

HOGENOMP16430.
OMAP16430. FFPWGQM.

Enzyme and pathway databases

BioCycEcoCyc:HYCD-MON.
MetaCyc:HYCD-MON.

Family and domain databases

InterProIPR001694. NADH_UbQ_OxRdtase_su1.
IPR018086. NADH_UbQ_OxRdtase_su1_CS.
[Graphical view]
PANTHERPTHR11432. Resp_NADH_DH_1. 1 hit.
PfamPF00146. NADHdh. 1 hit.
[Graphical view]
PROSITEPS00667. COMPLEX1_ND1_1. 1 hit.
PS00668. COMPLEX1_ND1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameHYCD_ECOLI
AccessionPrimary (citable) accession number: P16430
Secondary accession number(s): Q2MAA7
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: June 16, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents