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P16429 (HYCC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Formate hydrogenlyase subunit 3
Short name=FHL subunit 3
Alternative name(s):
Hydrogenase-3 component C
Gene names
Name:hycC
Synonyms:hevC
Ordered Locus Names:b2723, JW2693
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length608 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Subunit structure

FHL comprises of a formate dehydrogenase, unidentified electron carriers and a hydrogenase (isoenzyme 3). In this non-energy conserving pathway molecular hydrogen and carbodioxide from formate are released.

Subcellular location

Cell inner membrane; Multi-pass membrane protein Ref.4.

Sequence similarities

Belongs to the complex I subunit 4 family.

Ontologies

Keywords
   Cellular componentCell inner membrane
Cell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processATP synthesis coupled electron transport

Inferred from electronic annotation. Source: InterPro

   Cellular_componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionNADH dehydrogenase (ubiquinone) activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 608608Formate hydrogenlyase subunit 3
PRO_0000118048

Regions

Transmembrane10 – 2617Helical; Potential
Transmembrane44 – 6724Helical; Potential
Transmembrane76 – 9318Helical; Potential
Transmembrane116 – 14025Helical; Potential
Transmembrane153 – 17321Helical; Potential
Transmembrane197 – 21822Helical; Potential
Transmembrane229 – 25123Helical; Potential
Transmembrane258 – 28023Helical; Potential
Transmembrane296 – 31217Helical; Potential
Transmembrane416 – 44025Helical; Potential
Transmembrane453 – 47624Helical; Potential
Transmembrane502 – 52120Helical; Potential

Experimental info

Sequence conflict2271A → T in CAA35548. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P16429 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: 7E4F4C63626289B8

FASTA60864,077
        10         20         30         40         50         60 
MSAISLINSG VAWFVAAAVL AFLFSFQKAL SGWIAGIGGA VGSLYTAAAG FTVLTGAVGV 

        70         80         90        100        110        120 
SGALSLVSYD VQISPLNAIW LITLGLCGLF VSLYNIDWHR HAQVKCNGLQ INMLMAAAVC 

       130        140        150        160        170        180 
AVIASNLGMF VVMAEIMALC AVFLTSNSKE GKLWFALGRL GTLLLAIACW LLWQRYGTLD 

       190        200        210        220        230        240 
LRLLDMRMQQ LPLGSDIWLL GVIGFGLLAG IIPLHGWVPQ AHANASAPAA ALFSTVVMKI 

       250        260        270        280        290        300 
GLLGILTLSL LGGNAPLWWG IALLVLGMIT AFVGGLYALV EHNIQRLLAY HTLENIGIIL 

       310        320        330        340        350        360 
LGLGAGVTGI ALEQPALIAL GLVGGLYHLL NHSLFKSVLF LGAGSVWFRT GHRDIEKLGG 

       370        380        390        400        410        420 
IGKKMPVISI AMLVGLMAMA ALPPLNGFAG EWVIYQSFFK LSNSGAFVAR LLGPLLAVGL 

       430        440        450        460        470        480 
AITGALAVMC MAKVYGVTFL GAPRTKEAEN ATCAPLLMSV SVVALAICCV IGGVAAPWLL 

       490        500        510        520        530        540 
PMLSAAVPLP LEPANTTVSQ PMITLLLIAC PLLPFIIMAI CKGDRLPSRS RGAAWVCGYD 

       550        560        570        580        590        600 
HEKSMVITAH GFAMPVKQAF APVLKLRKWL NPVSLVPGWQ CEGSALLFRR MALVELAVLV 


VIIVSRGA

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence and expression of an operon in Escherichia coli coding for formate hydrogenlyase components."
Boehm R., Sauter M., Boeck A.
Mol. Microbiol. 4:231-243(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"Global topology analysis of the Escherichia coli inner membrane proteome."
Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
Strain: K12 / MG1655 / ATCC 47076.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X17506 Genomic DNA. Translation: CAA35548.1.
U29579 Genomic DNA. Translation: AAA69233.1.
U00096 Genomic DNA. Translation: AAC75765.1.
AP009048 Genomic DNA. Translation: BAE76800.1.
PIRG65052.
RefSeqNP_417203.1. NC_000913.2.
YP_490932.1. NC_007779.1.

3D structure databases

ProteinModelPortalP16429.
SMRP16429. Positions 203-401.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-9973N.
IntActP16429. 1 interaction.
MINTMINT-1240211.
STRING511145.b2723.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC75765; AAC75765; b2723.
BAE76800; BAE76800; BAE76800.
GeneID12933110.
945327.
KEGGecj:Y75_p2661.
eco:b2723.
PATRIC32120846. VBIEscCol129921_2815.

Organism-specific databases

EchoBASEEB0471.
EcoGeneEG10476. hycC.

Phylogenomic databases

eggNOGCOG0651.
HOGENOMHOG000278686.
KOK15828.
OMAMEYSSAG.
ProtClustDBPRK08042.

Enzyme and pathway databases

BioCycEcoCyc:HYCC-MONOMER.
ECOL316407:JW2693-MONOMER.
MetaCyc:HYCC-MONOMER.

Gene expression databases

GenevestigatorP16429.

Family and domain databases

InterProIPR001750. NADH_UbQ/plastoQ_OxRdtase.
IPR003918. NADH_UbQ_OxRdtase.
[Graphical view]
PfamPF00361. Oxidored_q1. 1 hit.
[Graphical view]
PRINTSPR01437. NUOXDRDTASE4.
ProtoNetSearch...

Entry information

Entry nameHYCC_ECOLI
AccessionPrimary (citable) accession number: P16429
Secondary accession number(s): Q2MAA6, Q46882
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: November 1, 1997
Last modified: May 1, 2013
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

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