##gff-version 3 ##sequence-region P16422 1 314 P16422 UniProtKB Signal peptide 1 23 . . . status=Potential P16422 UniProtKB Chain 24 314 . . . ID=PRO_0000022467;Note=Epithelial cell adhesion molecule P16422 UniProtKB Topological domain 24 265 . . . Note=Extracellular;status=Potential P16422 UniProtKB Transmembrane 266 288 . . . Note=Helical%3B;status=Potential P16422 UniProtKB Topological domain 289 314 . . . Note=Cytoplasmic;status=Potential P16422 UniProtKB Domain 63 135 . . . Note=Thyroglobulin type-1 P16422 UniProtKB Modified residue 24 24 . . . Note=Pyrrolidone carboxylic acid;status=Probable P16422 UniProtKB Glycosylation 74 74 . . . Note=N-linked (GlcNAc...)%3B partial P16422 UniProtKB Glycosylation 111 111 . . . Note=N-linked (GlcNAc...) P16422 UniProtKB Glycosylation 198 198 . . . Note=N-linked (GlcNAc...) P16422 UniProtKB Disulfide bond 27 46 . . . . P16422 UniProtKB Disulfide bond 29 59 . . . . P16422 UniProtKB Disulfide bond 38 48 . . . . P16422 UniProtKB Disulfide bond 66 99 . . . . P16422 UniProtKB Disulfide bond 110 116 . . . . P16422 UniProtKB Disulfide bond 118 135 . . . . P16422 UniProtKB Natural variant 66 66 . . . ID=VAR_063829;Note=In DIAR5. P16422 UniProtKB Natural variant 115 115 . . . ID=VAR_018329 P16422 UniProtKB Mutagenesis 74 74 . . . Note=Changed glycosylation pattern. Complete loss of glycosylation and substantial decrease in protein expression%3B when associated with A-111 and A-198. P16422 UniProtKB Mutagenesis 111 111 . . . Note=Changed glycosylation pattern. Complete loss of glycosylation and substantial decrease in protein expression%3B when associated with A-74 and A-198. P16422 UniProtKB Mutagenesis 198 198 . . . Note=Decreased glycosyation%2C reduced protein stability and significant decrease in protein expression. Complete loss of glycosylation and substantial decrease in protein expression%3B when associated with A-74 and A-111. P16422 UniProtKB Sequence conflict 277 277 . . . . P16422 UniProtKB Sequence conflict 277 277 . . . . P16422 UniProtKB Sequence conflict 277 277 . . . . P16422 UniProtKB Sequence conflict 303 303 . . . .