Reviewed, UniProtKB/Swiss-Prot P16422 (EPCAM_HUMAN)

Names and origin

Protein names

Recommended name:
    Epithelial cell adhesion molecule
      Short name=Ep-CAM
Alternative name(s):
    Tumor-associated calcium signal transducer 1
    Major gastrointestinal tumor-associated protein GA733-2
    Epithelial cell surface antigen
    Epithelial glycoprotein
      Short name=EGP
    Epithelial glycoprotein 314
      Short name=EGP314
      Short name=hEGP314
    Adenocarcinoma-associated antigen
    KSA
    KS 1/4 antigen
    Cell surface glycoprotein Trop-1
    CD_antigen=CD326

Gene names

Name:	EPCAM
Synonyms:	GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection By similarity.
Subunit structure	Monomer. Ref.11
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	This protein is expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinomas.
Sequence similarities	Belongs to the EPCAM family. Contains 1 thyroglobulin type-1 domain.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Repeat Signal Transmembrane
Molecular function	Tumor antigen
PTM	Disulfide bond Glycoprotein Pyrrolidone carboxylic acid
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Cellular component	apical plasma membrane Inferred from direct assay. Source: MGI basolateral plasma membrane Inferred from direct assay. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 314	291	Epithelial cell adhesion molecule		PRO_0000022467
Regions
	Topological domain	24 – 265	242	Extracellular Potential
	Transmembrane	266 – 288	23	Potential
	Topological domain	289 – 314	26	Cytoplasmic Potential
	Domain	63 – 135	73	Thyroglobulin type-1
Amino acid modifications
	Modified residue	24	1	Pyrrolidone carboxylic acid Probable
	Glycosylation	74	1	N-linked (GlcNAc...); partial Ref.12
	Glycosylation	111	1	N-linked (GlcNAc...) Ref.12
	Glycosylation	198	1	N-linked (GlcNAc...) Ref.13
	Disulfide bond	27 ↔ 46		Ref.12
	Disulfide bond	29 ↔ 59		Ref.12
	Disulfide bond	38 ↔ 48		Ref.12
	Disulfide bond	66 ↔ 99		Ref.12
	Disulfide bond	110 ↔ 116		Ref.12
	Disulfide bond	118 ↔ 135		Ref.12
Natural variations
	Natural variant	115	1	M → T: dbSNP rs1126497. Ref.1 Ref.2 Ref.3 Ref.9		VAR_018329
Experimental info
	Sequence conflict	277	1	I → M in AAA36151. Ref.1
	Sequence conflict	277	1	I → M in CAA32870. Ref.1
	Sequence conflict	277	1	I → M in AAA59543. Ref.2
	Sequence conflict	303	1	K → R in CAG47055. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P16422-1 [UniParc]. Last modified November 13, 2007. Version 2. Checksum: 023FCE418B2F1079	FASTA	314	34,932
	10 20 30 40 50 60 MAPPQVLAFG LLLAAATATF AAAQEECVCE NYKLAVNCFV NNNRQCQCTS VGAQNTVICS 70 80 90 100 110 120 KLAAKCLVMK AEMNGSKLGR RAKPEGALQN NDGLYDPDCD ESGLFKAKQC NGTSMCWCVN 130 140 150 160 170 180 TAGVRRTDKD TEITCSERVR TYWIIIELKH KAREKPYDSK SLRTALQKEI TTRYQLDPKF 190 200 210 220 230 240 ITSILYENNV ITIDLVQNSS QKTQNDVDIA DVAYYFEKDV KGESLFHSKK MDLTVNGEQL 250 260 270 280 290 300 DLDPGQTLIY YVDEKAPEFS MQGLKAGVIA VIVVVVIAVV AGIVVLVISR KKRMAKYEKA 310 EIKEMGEMHR ELNA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of a human adenocarcinoma/epithelial cell surface antigen complementary DNA." Strnad J., Hamilton A.E., Beavers L.S., Gamboa G.C., Apelgren L.D., Taber L.D., Sportsman J.R., Bumol T.F., Sharp J.D., Gadski R.A. Cancer Res. 49:314-317(1989) [PubMed: 2463074] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT THR-115. Tissue: Lung adenocarcinoma.
[2]	"Isolation and characterization of a cDNA encoding the KS1/4 epithelial carcinoma marker." Perez M.S., Walker L.E. J. Immunol. 142:3662-3667(1989) [PubMed: 2469722] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT THR-115.
[3]	"Epithelial glycoprotein is a member of a family of epithelial cell surface antigens homologous to nidogen, a matrix adhesion protein." Simon B., Podolsky D.K., Moldenhauer G., Isselbacher K.J., Gattoni-Celli S., Brand S.J. Proc. Natl. Acad. Sci. U.S.A. 87:2755-2759(1990) [PubMed: 2108441] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT THR-115.
[4]	"Molecular cloning of cDNA for the carcinoma-associated antigen GA733-2." Szala S., Froehlich M., Scollon M., Kasai Y., Steplewski Z., Koprowski H., Linnenbach A.J. Proc. Natl. Acad. Sci. U.S.A. 87:3542-3546(1990) [PubMed: 2333300] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Colon carcinoma.
[5]	"Retroposition in a family of carcinoma-associated antigen genes." Linnenbach A.J., Seng B.A., Wu S., Robbins S., Scollon M., Pyrc J.J., Druck T., Huebner K. Mol. Cell. Biol. 13:1507-1515(1993) [PubMed: 8382772] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Lymphoma.
[6]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[9]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT THR-115. Tissue: Ovary.
[10]	"Sequence investigation of the major gastrointestinal tumor-associated antigen gene family, GA733." Linnenbach A.J., Wojcierowski J., Wu S., Pyrc J.J., Ross A.H., Dietzschold B., Speicher D., Koprowski H. Proc. Natl. Acad. Sci. U.S.A. 86:27-31(1989) [PubMed: 2911574] [Abstract] Cited for: PRELIMINARY PARTIAL NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 81-126. Tissue: Placenta.
[11]	"Isolation, partial characterization, and molecular cloning of a human colon adenocarcinoma cell-surface glycoprotein recognized by the C215 mouse monoclonal antibody." Bjoerk P., Joensson U., Svedberg H., Larsson K., Lind P., Dillner J., Hedlund G., Dohlsten M., Kalland T. J. Biol. Chem. 268:24232-24241(1993) [PubMed: 7693697] [Abstract] Cited for: PROTEIN SEQUENCE OF 82-100, SUBUNIT.
[12]	"Determination of disulfide bond assignments and N-glycosylation sites of the human gastrointestinal carcinoma antigen GA733-2 (CO17-1A, EGP, KS1-4, KSA, and Ep-CAM)." Chong J.M., Speicher D.W. J. Biol. Chem. 276:5804-5813(2001) [PubMed: 11080501] [Abstract] Cited for: DISULFIDE BONDS, GLYCOSYLATION AT ASN-74 AND ASN-111.
[13]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed: 19159218] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-198, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases
EMBL GenBank DDBJ	M32325 mRNA. Translation: AAA36151.1. X14758 mRNA. Translation: CAA32870.1. M26481 mRNA. Translation: AAA59543.1. M32306 mRNA. Translation: AAA35723.1. M33011 mRNA. Translation: AAA35861.1. M93036 , M93029, M93030, M93031, M93032, M93033, M93034, M93035 Genomic DNA. Translation: AAB00775.1. CR542259 mRNA. Translation: CAG47055.1. CR542283 mRNA. Translation: CAG47078.1. AC079775 Genomic DNA. Translation: AAY15095.1. CH471053 Genomic DNA. Translation: EAX00218.1. BC014785 mRNA. Translation: AAH14785.1.
IPI	IPI00296215.
PIR	B48149.
RefSeq	NP_002345.2.
UniGene	Hs.542050
3D structure databases
SMR	P16422. Positions 91-137.
ModBase	Search...
Protein-protein interaction databases
STRING	P16422.
Proteomic databases
PRIDE	P16422.
Genome annotation databases
Ensembl	ENST00000263735; ENSP00000263735; ENSG00000119888; Homo sapiens. [Genome view]
GeneID	4072.
KEGG	hsa:4072.
Organism-specific databases
CTD	4072.
GeneCards	GC02P047425.
H-InvDB	HIX0002040.
HGNC	HGNC:11529. EPCAM.
HPA	CAB003809. HPA026761.
MIM	185535. gene.
PharmGKB	PA35493.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG14941.
HOVERGEN	P16422.
PhylomeDB	P16422.
Gene expression databases
ArrayExpress	P16422.
Bgee	P16422.
CleanEx	HS_EPCAM.
Genevestigator	P16422.
GermOnline	ENSG00000119888. Homo sapiens.
Family and domain databases
InterPro	IPR000716. Thyroglobulin_1. [Graphical view]
Gene3D	G3DSA:4.10.800.10. Thyroglobulin_1. 1 hit.
Pfam	PF00086. Thyroglobulin_1. 1 hit. [Graphical view]
SMART	SM00211. TY. 1 hit. [Graphical view]
PROSITE	PS00484. THYROGLOBULIN_1_1. 1 hit. PS51162. THYROGLOBULIN_1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

EPCAM_HUMAN

Accession

Primary (citable) accession number: P16422
Secondary accession number(s): P18180 , Q6FG26, Q6FG49, Q96C47, Q9UCD0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	November 13, 2007
Last modified:	January 19, 2010
This is version 102 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.