P16414 (RNAS1_SPAEH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease pancreatic
EC=3.1.27.5

Alternative name(s):
RNase 1
RNase A

Gene names

Name:	RNASE1
Synonyms:	RNS1

Organism

Spalax ehrenbergi (Middle East blind mole rat) (Nannospalax ehrenbergi)

Taxonomic identifier

30637 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Spalacidae › Spalacinae › Nannospalax

Protein attributes

Sequence length	125 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA By similarity.
Catalytic activity	Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.
Subunit structure	Monomer. Interacts with and forms tight 1:1 complexes with RNH1. Dimerization of two such complexes may occur. Interaction with RNH1 inhibits this protein By similarity.
Subcellular location	Secreted.
Tissue specificity	Pancreas.
Sequence similarities	Belongs to the pancreatic ribonuclease family.

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro pancreatic ribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 125	125	Ribonuclease pancreatic		PRO_0000057215
Regions
	Region	42 – 46	5	Substrate binding By similarity
Sites
	Active site	12	1	Proton acceptor By similarity
	Active site	120	1	Proton donor By similarity
	Binding site	7	1	Substrate By similarity
	Binding site	10	1	Substrate By similarity
	Binding site	67	1	Substrate By similarity
	Binding site	86	1	Substrate By similarity
Amino acid modifications
	Glycosylation	35	1	N-linked (GlcNAc...) Ref.1
	Disulfide bond	27 ↔ 85		By similarity
	Disulfide bond	41 ↔ 96		By similarity
	Disulfide bond	59 ↔ 111		By similarity
	Disulfide bond	66 ↔ 73		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P16414 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: 9D07C4F74261E7E4	FASTA	125	14,031
	10 20 30 40 50 60 KESSAEKFLR QHVDSIDSPG NLSPTYCNQM MLRRNMTKGS CKPVNTFVHE PLKDIKAVCF 70 80 90 100 110 120 QENVTCKNGN SNCYKSHASL HITDCRTISS SKYPDCAYKT SQEQKHIIVA CEGDPFVPVH YDASV

References

[1]	"The amino-acid sequence of pancreatic ribonuclease from the mole rat Spalax ehrenbergi, chromosomal species 2n = 60." Schueller C., Neuteboom B., Wuebbels G., Beintema J.J., Nevo E. Biol. Chem. Hoppe-Seyler 370:583-589(1989) [PubMed: 2673297] [Abstract] Cited for: PROTEIN SEQUENCE, GLYCOSYLATION AT ASN-35. Tissue: Pancreas.
+	Additional computationally mapped references.

Cross-references

Sequence databases
PIR	S04503.
3D structure databases
ProteinModelPortal	P16414.
SMR	P16414. Positions 1-125.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG008396.
Family and domain databases
InterPro	IPR001427. RNaseA. IPR023411. RNaseA_AS. IPR023412. RNaseA_domain. [Graphical view]
Gene3D	G3DSA:3.10.130.10. RNaseA. 1 hit.
PANTHER	PTHR11437. RNaseA. 1 hit.
Pfam	PF00074. RnaseA. 1 hit. [Graphical view]
PRINTS	PR00794. RIBONUCLEASE.
ProDom	PD000535. RNaseA. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00092. RNAse_Pc. 1 hit. [Graphical view]
SUPFAM	SSF54076. RNaseA. 1 hit.
PROSITE	PS00127. RNASE_PANCREATIC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNAS1_SPAEH

Accession

Primary (citable) accession number: P16414

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	June 28, 2011
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program