P16409 (MYL3_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Myosin light chain 3

Alternative name(s):
Myosin light chain 1, slow-twitch muscle B/ventricular isoform
Short name=MLC1SB
Ventricular/slow twitch myosin alkali light chain

Gene names

Name:	Myl3
Synonyms:	Mlc1v

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	200 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Regulatory light chain of myosin. Does not bind calcium.
Subunit structure	Myosin is a hexamer of 2 heavy chains and 4 light chains.
Post-translational modification	N-terminus is methylated by METTL11A/NTM1 By similarity.
Sequence similarities	Contains 3 EF-hand domains.

Ontologies

Keywords
Domain	Repeat
Molecular function	Motor protein Muscle protein Myosin
PTM	Methylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cardiac muscle contraction Inferred from direct assay PubMed 14659803PubMed 2044768. Source: RGD regulation of striated muscle contraction Inferred from electronic annotation. Source: Compara regulation of the force of heart contraction Inferred from electronic annotation. Source: Compara skeletal muscle tissue development Inferred from expression pattern Ref.1. Source: RGD ventricular cardiac muscle tissue morphogenesis Inferred from electronic annotation. Source: Compara
Cellular_component	A band Inferred from electronic annotation. Source: Compara I band Inferred from electronic annotation. Source: Compara myosin complex Inferred from direct assay PubMed 14659803PubMed 2044768. Source: RGD
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro motor activity Inferred from direct assay PubMed 14659803. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 200	199	Myosin light chain 3		PRO_0000198698
Regions
	Domain	54 – 91	38	EF-hand 1
	Domain	133 – 168	36	EF-hand 2
	Domain	168 – 200	33	EF-hand 3
Amino acid modifications
	Modified residue	2	1	N,N,N-trimethylalanine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P16409 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 2B8C832A9D364253	FASTA	200	22,156
	10 20 30 40 50 60 MAPKKPEPKK DDAKTAAPKA APAPAAAPAA APEPERPKEA EFDASKIKIE FTPEQIEEFK 70 80 90 100 110 120 EAFQLFDRTP KGEMKITYGQ CGDVLRALGQ NPTQAEVLRV LGKPKQEELN SKMMDFETFL 130 140 150 160 170 180 PMLQHISKNK DTGTYEDFVE GLRVFDKEGN GTVMGAELRH VLATLGERLT EDEVEKLMAG 190 200 QEDSNGCINY EAFVKHIMAS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Ventricular myosin light chain 1 is developmentally regulated and does not change in hypertension." McNally E., Buttrick P., Leinwand L. Nucleic Acids Res. 17:2753-2767(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Heart ventricle.
[2]	"Characterization of a rat myosin alkali light chain gene expressed in ventricular and slow twitch skeletal muscles." Periasamy M., Wodgaonkar R., Kumar C., Martin B.J., Siddiqui M.A.Q. Nucleic Acids Res. 17:7723-7734(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Wistar. Tissue: Heart ventricle.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Heart.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X14812 mRNA. Translation: CAA32917.1. X16325 , X16326, X16327, X16329, X16330 Genomic DNA. Translation: CAA34388.1. BC081832 mRNA. Translation: AAH81832.1.
IPI	IPI00231788.
PIR	MORT3V. S09573.
RefSeq	NP_036738.1. NM_012606.2.
UniGene	Rn.1955.
3D structure databases
ProteinModelPortal	P16409.
SMR	P16409. Positions 50-199.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000028458.
PTM databases
PhosphoSite	P16409.
Proteomic databases
PaxDb	P16409.
PRIDE	P16409.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000028458; ENSRNOP00000028458; ENSRNOG00000020955.
GeneID	24585.
KEGG	rno:24585.
Organism-specific databases
CTD	4634.
RGD	3142. Myl3.
Phylogenomic databases
eggNOG	COG5126.
GeneTree	ENSGT00590000082921.
HOGENOM	HOG000233018.
HOVERGEN	HBG012180.
InParanoid	P16409.
KO	K12749.
OMA	KMMDFDT.
OrthoDB	EOG483D5S.
Gene expression databases
Genevestigator	P16409.
GermOnline	ENSRNOG00000020955. Rattus norvegicus.
Family and domain databases
Gene3D	1.10.238.10. 2 hits.
InterPro	IPR011992. EF-hand-like_dom. IPR002048. EF_hand_dom. [Graphical view]
Pfam	PF13405. EF_hand_4. 1 hit. [Graphical view]
SMART	SM00054. EFh. 2 hits. [Graphical view]
PROSITE	PS50222. EF_HAND_2. 3 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	603758.

Entry information

Entry name

MYL3_RAT

Accession

Primary (citable) accession number: P16409

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 99 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program