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Protein

Myosin light chain 3

Gene

Myl3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Regulatory light chain of myosin. Does not bind calcium.

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • motor activity Source: RGD

GO - Biological processi

  • cardiac muscle contraction Source: RGD
  • metabolic process Source: GOC
  • muscle contraction Source: RGD
  • regulation of striated muscle contraction Source: Ensembl
  • regulation of the force of heart contraction Source: Ensembl
  • skeletal muscle tissue development Source: RGD
  • ventricular cardiac muscle tissue morphogenesis Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Motor protein, Muscle protein, Myosin

Enzyme and pathway databases

ReactomeiREACT_283017. Striated Muscle Contraction.

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin light chain 3
Alternative name(s):
Myosin light chain 1, slow-twitch muscle B/ventricular isoform
Short name:
MLC1SB
Ventricular/slow twitch myosin alkali light chain
Gene namesi
Name:Myl3
Synonyms:Mlc1v
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi3142. Myl3.

Subcellular locationi

GO - Cellular componenti

  • A band Source: Ensembl
  • I band Source: Ensembl
  • myosin complex Source: RGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 200199Myosin light chain 3PRO_0000198698Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N,N,N-trimethylalanineBy similarity

Post-translational modificationi

N-terminus is methylated by METTL11A/NTM1.By similarity

Keywords - PTMi

Methylation

Proteomic databases

PaxDbiP16409.
PRIDEiP16409.

PTM databases

PhosphoSiteiP16409.

Expressioni

Gene expression databases

GenevisibleiP16409. RN.

Interactioni

Subunit structurei

Myosin is a hexamer of 2 heavy chains and 4 light chains.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028458.

Structurei

3D structure databases

ProteinModelPortaliP16409.
SMRiP16409. Positions 50-199.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini54 – 9138EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini133 – 16836EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini168 – 20033EF-hand 3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 3 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5126.
GeneTreeiENSGT00590000082921.
HOGENOMiHOG000233018.
HOVERGENiHBG012180.
InParanoidiP16409.
KOiK12749.
OMAiKEVEFNP.
OrthoDBiEOG7HQN9N.
PhylomeDBiP16409.
TreeFamiTF351553.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13405. EF-hand_6. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 2 hits.
[Graphical view]
PROSITEiPS50222. EF_HAND_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P16409-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPKKPEPKK DDAKTAAPKA APAPAAAPAA APEPERPKEA EFDASKIKIE
60 70 80 90 100
FTPEQIEEFK EAFQLFDRTP KGEMKITYGQ CGDVLRALGQ NPTQAEVLRV
110 120 130 140 150
LGKPKQEELN SKMMDFETFL PMLQHISKNK DTGTYEDFVE GLRVFDKEGN
160 170 180 190 200
GTVMGAELRH VLATLGERLT EDEVEKLMAG QEDSNGCINY EAFVKHIMAS
Length:200
Mass (Da):22,156
Last modified:January 23, 2007 - v2
Checksum:i2B8C832A9D364253
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14812 mRNA. Translation: CAA32917.1.
X16325
, X16326, X16327, X16329, X16330 Genomic DNA. Translation: CAA34388.1.
BC081832 mRNA. Translation: AAH81832.1.
PIRiS09573. MORT3V.
RefSeqiNP_036738.1. NM_012606.2.
UniGeneiRn.1955.

Genome annotation databases

EnsembliENSRNOT00000028458; ENSRNOP00000028458; ENSRNOG00000020955.
GeneIDi24585.
KEGGirno:24585.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14812 mRNA. Translation: CAA32917.1.
X16325
, X16326, X16327, X16329, X16330 Genomic DNA. Translation: CAA34388.1.
BC081832 mRNA. Translation: AAH81832.1.
PIRiS09573. MORT3V.
RefSeqiNP_036738.1. NM_012606.2.
UniGeneiRn.1955.

3D structure databases

ProteinModelPortaliP16409.
SMRiP16409. Positions 50-199.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028458.

PTM databases

PhosphoSiteiP16409.

Proteomic databases

PaxDbiP16409.
PRIDEiP16409.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000028458; ENSRNOP00000028458; ENSRNOG00000020955.
GeneIDi24585.
KEGGirno:24585.

Organism-specific databases

CTDi4634.
RGDi3142. Myl3.

Phylogenomic databases

eggNOGiCOG5126.
GeneTreeiENSGT00590000082921.
HOGENOMiHOG000233018.
HOVERGENiHBG012180.
InParanoidiP16409.
KOiK12749.
OMAiKEVEFNP.
OrthoDBiEOG7HQN9N.
PhylomeDBiP16409.
TreeFamiTF351553.

Enzyme and pathway databases

ReactomeiREACT_283017. Striated Muscle Contraction.

Miscellaneous databases

NextBioi603758.
PROiP16409.

Gene expression databases

GenevisibleiP16409. RN.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13405. EF-hand_6. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 2 hits.
[Graphical view]
PROSITEiPS50222. EF_HAND_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Ventricular myosin light chain 1 is developmentally regulated and does not change in hypertension."
    McNally E., Buttrick P., Leinwand L.
    Nucleic Acids Res. 17:2753-2767(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Heart ventricle.
  2. "Characterization of a rat myosin alkali light chain gene expressed in ventricular and slow twitch skeletal muscles."
    Periasamy M., Wodgaonkar R., Kumar C., Martin B.J., Siddiqui M.A.Q.
    Nucleic Acids Res. 17:7723-7734(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Wistar.
    Tissue: Heart ventricle.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart.

Entry informationi

Entry nameiMYL3_RAT
AccessioniPrimary (citable) accession number: P16409
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: January 23, 2007
Last modified: July 22, 2015
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.