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Protein

Glutamyl aminopeptidase

Gene

Enpep

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Appears to have a role in the catabolic pathway of the renin-angiotensin system. Probably plays a role in regulating growth and differentiation of early B-lineage cells.

Catalytic activityi

Release of N-terminal glutamate (and to a lesser extent aspartate) from a peptide.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei215 – 2151SubstrateBy similarity
Metal bindingi385 – 3851Zinc; catalyticPROSITE-ProRule annotation
Active sitei386 – 3861Proton acceptorPROSITE-ProRule annotation
Metal bindingi389 – 3891Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi408 – 4081Zinc; catalyticPROSITE-ProRule annotation
Sitei471 – 4711Transition state stabilizerBy similarity

GO - Molecular functioni

GO - Biological processi

  • angiogenesis Source: MGI
  • cell migration Source: UniProtKB
  • cell proliferation Source: UniProtKB
  • glomerulus development Source: UniProtKB
  • peptide catabolic process Source: GO_Central
  • regulation of blood pressure Source: GO_Central
  • regulation of systemic arterial blood pressure by renin-angiotensin Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.4.11.7. 3474.
ReactomeiR-MMU-2022377. Metabolism of Angiotensinogen to Angiotensins.

Protein family/group databases

MEROPSiM01.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl aminopeptidase (EC:3.4.11.7)
Short name:
EAP
Alternative name(s):
Aminopeptidase A
Short name:
AP-A
BP-1/6C3 antigen
CD_antigen: CD249
Gene namesi
Name:Enpep
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:106645. Enpep.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1818CytoplasmicSequence analysisAdd
BLAST
Transmembranei19 – 3921Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini40 – 945906ExtracellularSequence analysisAdd
BLAST

GO - Cellular componenti

  • apical part of cell Source: MGI
  • apical plasma membrane Source: MGI
  • brush border Source: MGI
  • cytoplasmic vesicle Source: MGI
  • external side of plasma membrane Source: MGI
  • extracellular exosome Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • lysosomal membrane Source: MGI
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry

GuidetoPHARMACOLOGYi1568.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 945945Glutamyl aminopeptidasePRO_0000095096Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi116 – 1161N-linked (GlcNAc...)Sequence analysis
Glycosylationi189 – 1891N-linked (GlcNAc...)Sequence analysis
Glycosylationi316 – 3161N-linked (GlcNAc...)Sequence analysis
Glycosylationi546 – 5461N-linked (GlcNAc...)Sequence analysis
Glycosylationi601 – 6011N-linked (GlcNAc...)Sequence analysis
Glycosylationi637 – 6371N-linked (GlcNAc...)Sequence analysis
Glycosylationi669 – 6691N-linked (GlcNAc...)Sequence analysis
Glycosylationi754 – 7541N-linked (GlcNAc...)Sequence analysis
Glycosylationi792 – 7921N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP16406.
PeptideAtlasiP16406.
PRIDEiP16406.

PTM databases

iPTMnetiP16406.
PhosphoSiteiP16406.

Expressioni

Tissue specificityi

Early B-lineage cells and certain stromal cell of hemopoietic tissues. Also expressed by capillary endothelial cells, placenta, and epithelial cells of the intestine and proximal renal tubules.

Gene expression databases

BgeeiENSMUSG00000028024.
CleanExiMM_ENPEP.
ExpressionAtlasiP16406. baseline and differential.
GenevisibleiP16406. MM.

Interactioni

Subunit structurei

Homodimer; disulfide-linked.

Protein-protein interaction databases

IntActiP16406. 2 interactions.
MINTiMINT-4087658.
STRINGi10090.ENSMUSP00000029658.

Structurei

3D structure databases

ProteinModelPortaliP16406.
SMRiP16406. Positions 68-944.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni349 – 3535Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the peptidase M1 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1046. Eukaryota.
COG0308. LUCA.
HOGENOMiHOG000106482.
HOVERGENiHBG006616.
InParanoidiP16406.
KOiK11141.
OMAiQVQVRRC.
OrthoDBiEOG091G01GH.
PhylomeDBiP16406.
TreeFamiTF300395.

Family and domain databases

InterProiIPR033508. Aminopeptidase_A.
IPR024571. ERAP1-like_C_dom.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
[Graphical view]
PANTHERiPTHR11533. PTHR11533. 1 hit.
PTHR11533:SF188. PTHR11533:SF188. 1 hit.
PfamiPF11838. ERAP1_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view]
PRINTSiPR00756. ALADIPTASE.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P16406-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNFAEEEPSK KYCIKGKHVA IICGVVVAVG LIVGLSVGLT RSCEQDTTPA
60 70 80 90 100
PSQPPPEAST ALPPQDQNVC PDSEDESGEW KNFRLPDFIN PVHYDLEVKA
110 120 130 140 150
LMEEDRYTGI VTISVNLSKP TRDLWLHIRE TKITKLPELR RPSGEQVPIR
160 170 180 190 200
RCFEYKKQEY VVIQAAEDLA ATSGDSVYRL TMEFKGWLNG SLVGFYKTTY
210 220 230 240 250
MEDGQIRSIA ATDHEPTDAR KSFPCFDEPN KKSTYSISII HPKEYSALSN
260 270 280 290 300
MPEEKSEMVD DNWKKTTFVK SVPMSTYLVC FAVHRFTAIE RKSRSGKPLK
310 320 330 340 350
VYVQPNQKET AEYAANITQA VFDYFEDYFA MEYALPKLDK IAIPDFGTGA
360 370 380 390 400
MENWGLVTYR ETNLLYDPLL SASSNQQRVA SVVAHELVHQ WFGNTVTMDW
410 420 430 440 450
WDDLWLNEGF ASFFEFLGVN HAEKDWQMLS QVLLEDVFPV QEDDSLMSSH
460 470 480 490 500
PVVVTVSTPA EITSVFDGIS YSKGASILRM LQDWITPEKF QKGCQIYLKK
510 520 530 540 550
FQFANAKTSD FWDSLQEASN LPVKEVMDTW TSQMGYPVVT VSGRQNITQK
560 570 580 590 600
RFLLDSKADP SQPPSELGYT WNIPVRWADN DNSRITVYNR LDKGGITLNA
610 620 630 640 650
NLSGDAFLKI NPDHIGFYRV NYEGGTWDWI AEALSSNHTR FSAADRSSFI
660 670 680 690 700
DDAFALARAQ LLNYKIALNL TMYLKSEEDF LPWERVISSV SYIISMFEDD
710 720 730 740 750
RELYPMIETY FQGQVKPVAD LLGWQDTGSH ITKLLRASIL GFACKMGDRE
760 770 780 790 800
ALGNASQLFD SWLKGSASIP VNLRLLVYRY GMQNSGNEAA WNYTLEQYQK
810 820 830 840 850
TSLAQEKEKL LYGLASVKDV KLLARYLEML KDPNIIKTQD VFTVIRYISY
860 870 880 890 900
NSYGKTMAWN WIQLNWDYLV SRFTINDRYL GRIVTIAEPF NTELQLWQMQ
910 920 930 940
SFFAKYPNAG AGAKPREQVL ETVKNNIEWL NVNRQSIREW FASLP
Length:945
Mass (Da):107,956
Last modified:August 1, 1990 - v1
Checksum:iA93A00CB8635F574
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M29961 mRNA. Translation: AAB47732.1.
CCDSiCCDS17831.1.
PIRiS30398.
RefSeqiNP_031960.1. NM_007934.3.
UniGeneiMm.1193.

Genome annotation databases

EnsembliENSMUST00000029658; ENSMUSP00000029658; ENSMUSG00000028024.
GeneIDi13809.
KEGGimmu:13809.
UCSCiuc008rhy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M29961 mRNA. Translation: AAB47732.1.
CCDSiCCDS17831.1.
PIRiS30398.
RefSeqiNP_031960.1. NM_007934.3.
UniGeneiMm.1193.

3D structure databases

ProteinModelPortaliP16406.
SMRiP16406. Positions 68-944.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP16406. 2 interactions.
MINTiMINT-4087658.
STRINGi10090.ENSMUSP00000029658.

Chemistry

GuidetoPHARMACOLOGYi1568.

Protein family/group databases

MEROPSiM01.003.

PTM databases

iPTMnetiP16406.
PhosphoSiteiP16406.

Proteomic databases

PaxDbiP16406.
PeptideAtlasiP16406.
PRIDEiP16406.

Protocols and materials databases

DNASUi13809.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029658; ENSMUSP00000029658; ENSMUSG00000028024.
GeneIDi13809.
KEGGimmu:13809.
UCSCiuc008rhy.1. mouse.

Organism-specific databases

CTDi2028.
MGIiMGI:106645. Enpep.

Phylogenomic databases

eggNOGiKOG1046. Eukaryota.
COG0308. LUCA.
HOGENOMiHOG000106482.
HOVERGENiHBG006616.
InParanoidiP16406.
KOiK11141.
OMAiQVQVRRC.
OrthoDBiEOG091G01GH.
PhylomeDBiP16406.
TreeFamiTF300395.

Enzyme and pathway databases

BRENDAi3.4.11.7. 3474.
ReactomeiR-MMU-2022377. Metabolism of Angiotensinogen to Angiotensins.

Miscellaneous databases

PROiP16406.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028024.
CleanExiMM_ENPEP.
ExpressionAtlasiP16406. baseline and differential.
GenevisibleiP16406. MM.

Family and domain databases

InterProiIPR033508. Aminopeptidase_A.
IPR024571. ERAP1-like_C_dom.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
[Graphical view]
PANTHERiPTHR11533. PTHR11533. 1 hit.
PTHR11533:SF188. PTHR11533:SF188. 1 hit.
PfamiPF11838. ERAP1_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view]
PRINTSiPR00756. ALADIPTASE.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAMPE_MOUSE
AccessioniPrimary (citable) accession number: P16406
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: September 7, 2016
This is version 159 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.