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Reviewed, UniProtKB/Swiss-Prot P16406 (AMPE_MOUSE)

Last modified June 16, 2009. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Glutamyl aminopeptidase
    EC=3.4.11.7
Alternative name(s):
    EAP
    Aminopeptidase A
      Short name=APA
    BP-1/6C3 antigen
    CD_antigen=CD249
Gene names
Name: Enpep
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length945 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Appears to have a role in the catabolic pathway of the renin-angiotensin system. Probably plays a role in regulating growth and differentiation of early B-lineage cells.

Catalytic activity

Release of N-terminal glutamate (and to a lesser extent aspartate) from a peptide.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Subunit structure

Homodimer; disulfide-linked.

Subcellular location

Membrane; Single-pass type II membrane protein.

Tissue specificity

Early B-lineage cells and certain stromal cell of hemopoietic tissues. Also expressed by capillary endothelial cells, placenta, and epithelial cells of the intestine and proximal renal tubules.

Sequence similarities

Belongs to the peptidase M1 family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 945945Glutamyl aminopeptidase
PRO_0000095096

Regions

Topological domain1 – 1818Cytoplasmic Potential
Transmembrane19 – 3921Signal-anchor for type II membrane protein Potential
Topological domain40 – 945906Extracellular Potential

Sites

Active site3861 By similarity
Active site4711Proton donor Potential
Metal binding3851Zinc; catalytic By similarity
Metal binding3891Zinc; catalytic By similarity
Metal binding4081Zinc; catalytic By similarity

Amino acid modifications

Modified residue121Phosphotyrosine By similarity
Glycosylation1161N-linked (GlcNAc...) Potential
Glycosylation1891N-linked (GlcNAc...) Potential
Glycosylation3161N-linked (GlcNAc...) Potential
Glycosylation5461N-linked (GlcNAc...) Potential
Glycosylation6011N-linked (GlcNAc...) Potential
Glycosylation6371N-linked (GlcNAc...) Potential
Glycosylation6691N-linked (GlcNAc...) Potential
Glycosylation7541N-linked (GlcNAc...) Potential
Glycosylation7921N-linked (GlcNAc...) Potential

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Sequences

Sequence LengthMass (Da)Tools
P16406-1 [UniParc].

Last modified August 1, 1990. Version 1.
Checksum: A93A00CB8635F574

FASTA945107,956
        10         20         30         40         50         60 
MNFAEEEPSK KYCIKGKHVA IICGVVVAVG LIVGLSVGLT RSCEQDTTPA PSQPPPEAST 

        70         80         90        100        110        120 
ALPPQDQNVC PDSEDESGEW KNFRLPDFIN PVHYDLEVKA LMEEDRYTGI VTISVNLSKP 

       130        140        150        160        170        180 
TRDLWLHIRE TKITKLPELR RPSGEQVPIR RCFEYKKQEY VVIQAAEDLA ATSGDSVYRL 

       190        200        210        220        230        240 
TMEFKGWLNG SLVGFYKTTY MEDGQIRSIA ATDHEPTDAR KSFPCFDEPN KKSTYSISII 

       250        260        270        280        290        300 
HPKEYSALSN MPEEKSEMVD DNWKKTTFVK SVPMSTYLVC FAVHRFTAIE RKSRSGKPLK 

       310        320        330        340        350        360 
VYVQPNQKET AEYAANITQA VFDYFEDYFA MEYALPKLDK IAIPDFGTGA MENWGLVTYR 

       370        380        390        400        410        420 
ETNLLYDPLL SASSNQQRVA SVVAHELVHQ WFGNTVTMDW WDDLWLNEGF ASFFEFLGVN 

       430        440        450        460        470        480 
HAEKDWQMLS QVLLEDVFPV QEDDSLMSSH PVVVTVSTPA EITSVFDGIS YSKGASILRM 

       490        500        510        520        530        540 
LQDWITPEKF QKGCQIYLKK FQFANAKTSD FWDSLQEASN LPVKEVMDTW TSQMGYPVVT 

       550        560        570        580        590        600 
VSGRQNITQK RFLLDSKADP SQPPSELGYT WNIPVRWADN DNSRITVYNR LDKGGITLNA 

       610        620        630        640        650        660 
NLSGDAFLKI NPDHIGFYRV NYEGGTWDWI AEALSSNHTR FSAADRSSFI DDAFALARAQ 

       670        680        690        700        710        720 
LLNYKIALNL TMYLKSEEDF LPWERVISSV SYIISMFEDD RELYPMIETY FQGQVKPVAD 

       730        740        750        760        770        780 
LLGWQDTGSH ITKLLRASIL GFACKMGDRE ALGNASQLFD SWLKGSASIP VNLRLLVYRY 

       790        800        810        820        830        840 
GMQNSGNEAA WNYTLEQYQK TSLAQEKEKL LYGLASVKDV KLLARYLEML KDPNIIKTQD 

       850        860        870        880        890        900 
VFTVIRYISY NSYGKTMAWN WIQLNWDYLV SRFTINDRYL GRIVTIAEPF NTELQLWQMQ 

       910        920        930        940 
SFFAKYPNAG AGAKPREQVL ETVKNNIEWL NVNRQSIREW FASLP

« Hide

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References

[1]"Molecular cloning of the murine BP-1/6C3 antigen: a member of the zinc-dependent metallopeptidase family."
Wu Q., Lahti J.M., Air G.M., Burrows P.D., Cooper M.D.
Proc. Natl. Acad. Sci. U.S.A. 87:993-997(1990) [PubMed: 1689065] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 77-96; 98-102; 624-632; 709-720 AND 930-939.
+Additional computationally mapped references.

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Cross-references

Sequence databases

M29961 mRNA. Translation: AAB47732.1.
IPIIPI00134585.
PIRS30398.
RefSeqNP_031960.1.
UniGeneMm.1193

3D structure databases

ModBaseSearch...

Protein family/group databases

MEROPSM01.003.

PTM databases

PhosphoSiteP16406.

Proteomic databases

PRIDEP16406.

Genome annotation databases

EnsemblENSMUSG00000028024. Mus musculus. [Contig view]
GeneID13809.
KEGGmmu:13809.

Organism-specific databases

MGIMGI:106645. Enpep.

Phylogenomic databases

HOGENOMP16406.
HOVERGENP16406.
OMAP16406. WITPEKF.

Enzyme and pathway databases

BRENDA3.4.11.7. 244.

Gene expression databases

ArrayExpressP16406.
BgeeP16406.
CleanExMM_ENPEP.
GermOnlineENSMUSG00000028024. Mus musculus.

Family and domain databases

InterProIPR006025. Pept_M_Zn_BS.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
[Graphical view]
PANTHERPTHR11533. Peptidase_M1. 1 hit.
PfamPF01433. Peptidase_M1. 1 hit.
[Graphical view]
PRINTSPR00756. ALADIPTASE.
PROSITEPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio284598.
SOURCESearch...

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Entry information

Entry nameAMPE_MOUSE
AccessionPrimary (citable) accession number: P16406
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: June 16, 2009
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents