P16393 (HMDH_STRPU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-hydroxy-3-methylglutaryl-coenzyme A reductase
Short name=HMG-CoA reductase
EC=1.1.1.34

Gene names

Name:

HMGCR

Organism

Strongylocentrotus purpuratus (Purple sea urchin)

Taxonomic identifier

7668 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Echinodermata › Eleutherozoa › Echinozoa › Echinoidea › Euechinoidea › Echinacea › Echinoida › Strongylocentrotidae › Strongylocentrotus

Protein attributes

Sequence length	932 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This transmembrane glycoprotein is involved in the control of cholesterol biosynthesis. It is the rate-limiting enzyme of sterol biosynthesis.
Catalytic activity	(R)-mevalonate + CoA + 2 NADP+ = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH.
Pathway	Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the HMG-CoA reductase family.

Ontologies

Keywords
Biological process	Cholesterol biosynthesis Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	cholesterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW coenzyme A metabolic process Inferred from electronic annotation. Source: InterPro isoprenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro hydroxymethylglutaryl-CoA reductase (NADPH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 932	932	3-hydroxy-3-methylglutaryl-coenzyme A reductase		PRO_0000114425
Regions
	Transmembrane	20 – 40	21	Helical; Potential
	Transmembrane	59 – 79	21	Helical; Potential
	Transmembrane	92 – 112	21	Helical; Potential
	Transmembrane	113 – 133	21	Helical; Potential
	Transmembrane	162 – 182	21	Helical; Potential
	Transmembrane	193 – 213	21	Helical; Potential
	Transmembrane	322 – 342	21	Helical; Potential
	Region	343 – 467	125	Linker
	Region	468 – 932	465	Catalytic
Sites
	Active site	575	1	Charge relay system By similarity
	Active site	707	1	Charge relay system By similarity
	Active site	783	1	Charge relay system By similarity
	Active site	882	1	Proton donor By similarity
Amino acid modifications
	Modified residue	888	1	Phosphoserine; by AMPK By similarity
	Glycosylation	279	1	N-linked (GlcNAc...) Potential
	Glycosylation	850	1	N-linked (GlcNAc...) Potential
	Glycosylation	886	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P16393 [UniParc]. Last modified November 1, 1990. Version 2. Checksum: A645677DE1142A7B	FASTA	932	100,966
	10 20 30 40 50 60 MLSRLFLAQG RFCSSHPWEV IVCTLTLTIC MLSMNYFTGL PRICGWNYEC APQVKESSLS 70 80 90 100 110 120 SDVLVMCIMR TLAVAYLYLQ FTKLRTTGSK YILGIAGLFT IFSSFLFSSA VIHLFGLELT 130 140 150 160 170 180 GLNEALPFFL LLIDLTKASA LTKFALSSTT QNEVVDNIAR GMAILGPTIT LDTVVTTLVI 190 200 210 220 230 240 SIGTMSSIRK MEVFCCFGIL SLIANYFVFM TFFPACLSLV LELSNSNKYG RPVWHLGRFA 250 260 270 280 290 300 EVLEEEEDRK PNPVVQRVKM IMRTGLVLVH AHSYWLASND TELMSRDMLY DGNLLTDKKI 310 320 330 340 350 360 DPTMPLWEFY ATRLWPPTLD YILTAILATV LASHYIFFSD LATYPEKRVS IMEGHEVVNP 370 380 390 400 410 420 GSDHEDASEV ETIGTLSSSP STSDVRVIES MTSRTQACQT DPVTASPRNS RSSSPVSSHS 430 440 450 460 470 480 VKPARFTIGS SGSGSEDEEE EVIKEEEVEW VLETELKAPR PMPELLEILN VGKGPNALTD 490 500 510 520 530 540 DEVQLLVGAK HIPAYKLENI LDNPERGVAV RRQIISKLLP ITDALEKLPY ASYDYSFVSG 550 560 570 580 590 600 ACCENVIGYM PVPVGVAGPL LLDGQEFQVP MATTEGCLVA STNRGCRALR SAGGIHSVLI 610 620 630 640 650 660 GDGMTRGPLV RLPSAQEAGA IKQWLEVPEN FAAIKERFES TSRFAKLKSI QTALAGRYMF 670 680 690 700 710 720 LRFKALTGDA MGMNMISKGT EQALHALQTM FPNIEIMSLS GNYCTDKKVA AINWIEGRGK 730 740 750 760 770 780 SVVCEATVPA HIVQQVLKTS ASALVDLNIH KNLVGSAMAG SIGGFNAHAA NIVTAIYIAT 790 800 810 820 830 840 GQDAAQNIAS SNCMTLMETR GPKGGDLYLS CTMPSIELGT VGGGTVLPPQ SACLQMMDVK 850 860 870 880 890 900 GSNIHGSGLN ASQLARIVCA TVMAGELSLM SALAAGHLVK SHMKHNRSAL NIASPLPSID 910 920 930 EVATHRRSKS VDFSALKESS AAAPGTCTAN AS

References

[1]	"3-hydroxy-3-methylglutaryl-coenzyme A reductase of the sea urchin embryo. Deduced structure and regulatory properties." Woodward H.D., Allen J.M.C., Lennarz W.J. J. Biol. Chem. 263:18411-18418(1988) [PubMed: 3192541] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"3-hydroxy-3-methylglutaryl coenzyme A reductase in the sea urchin embryo is developmentally regulated." Woodward H.D., Allen J.M.C., Lennarz W.J. J. Biol. Chem. 263:2513-2517(1988) [PubMed: 3276692] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 689-735.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J04200 mRNA. Translation: AAA30060.1. J03523 Genomic DNA. No translation available.
PIR	A31898.
RefSeq	NP_999724.1. NM_214559.1.
UniGene	Spu.2238.
3D structure databases
ProteinModelPortal	P16393.
SMR	P16393. Positions 459-881.
ModBase	Search...
Protein-protein interaction databases
STRING	P16393.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	373355.
KEGG	spu:373355.
Organism-specific databases
CTD	3156.
Phylogenomic databases
InParanoid	P16393.
PhylomeDB	P16393.
Family and domain databases
InterPro	IPR002202. HMG_CoA_Rdtase. IPR023074. HMG_CoA_Rdtase_cat. IPR023076. HMG_CoA_Rdtase_CS. IPR004554. HMG_CoA_Rdtase_eu_arc. IPR004816. HMG_CoA_Rdtase_metazoan. IPR023282. HMG_CoA_Rdtase_N. IPR009023. HMG_CoA_Rdtase_NAD(P)-bd. IPR009029. HMG_CoA_Rdtase_sub-bd. IPR000731. SSD. [Graphical view]
Gene3D	G3DSA:3.30.70.420. G3DSA:3.30.70.420. 1 hit. G3DSA:3.90.770.10. HMG-CoA_red. 2 hits. G3DSA:1.10.3270.10. HMG_CoA_Rdtase_N. 1 hit.
KO	K00021.
PANTHER	PTHR10572. HMG-CoA_red. 1 hit.
Pfam	PF00368. HMG-CoA_red. 1 hit. [Graphical view]
PRINTS	PR00071. HMGCOARDTASE.
SUPFAM	SSF55035. HMG_CoA_NAD_bind. 1 hit. SSF56542. HMG_CoA_sub_bind. 1 hit.
TIGRFAMs	TIGR00920. 2A060605. 1 hit. TIGR00533. HMG_CoA_R_NADP. 1 hit.
PROSITE	PS00066. HMG_COA_REDUCTASE_1. 1 hit. PS00318. HMG_COA_REDUCTASE_2. 1 hit. PS01192. HMG_COA_REDUCTASE_3. 1 hit. PS50065. HMG_COA_REDUCTASE_4. 1 hit. PS50156. SSD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HMDH_STRPU

Accession

Primary (citable) accession number: P16393

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	November 1, 1990
Last modified:	December 14, 2011
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)